FastQCFastQC Report
Fri 17 Jun 2016
SRR1527628_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527628_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1453597
Sequences flagged as poor quality0
Sequence length52
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCAACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC89280.6142004971116478No Hit
ATCAACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT53260.3664014166237272No Hit
ATCAACGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC47590.32739473182732215No Hit
ATCAACGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCGCC31320.2154654969706184No Hit
ATCAACGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGG30030.20659096021799717No Hit
ATCAACGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT28350.19503342398202528No Hit
ATCAACGGGGGGGCTGGAGAGATGGCTCAGAGGTTAAGAGCACTGACTGCTC26810.18443901576571772No Hit
ATCAACGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC25190.17329424868103058No Hit
ATCAACGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT22650.15582035460997787No Hit
ATCAACGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTCT22440.15437566258048138No Hit
ATCAACGGGGGGGTTGGATAGATGGCTCAGCAGTTAAGAGCACCGACTGCTC20970.14426281837400598No Hit
ATCAACGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA19970.13738333251926083No Hit
ATCAACGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTC15780.10855828678787863No Hit
ATCAACGGGACGTGGCTGACGATGACTGGTCTTCCTGGCCATATTACTTTCT14540.10002772432799463No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAG405.6134013E-946.019
TAACGGC206.312009E-446.012
GTAATCG206.312009E-446.022
CGTTACG206.312009E-446.030
CTAACGG301.8617447E-646.011
ACGTCGT301.8617447E-646.015
GATTGCG206.312009E-446.09
ATAACGG206.312009E-446.036
TTCGATA206.312009E-446.039
GCGTATT206.312009E-446.013
TACGTTG206.312009E-446.034
TACGTTA206.312009E-446.044
GACCGTA351.0198164E-745.99999623
CGGGTAT7750.045.4064526
CGGGCTA3200.044.56256
CAACGGG1446950.044.4851573
TCAACGG1480000.044.48482
AACGGGG776900.044.4279864
ACGGGGG316300.044.392985
AACGGGA426200.044.3648534
Produced by FastQC (version 0.11.5)