FastQCFastQC Report
Fri 17 Jun 2016
SRR1527621_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527621_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences78632
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7500.9538101536270222No Hit
TTTAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4260.5417641672601485No Hit
TTTAGGGGGCGGCGACGGTGGTGAGGGCCTTGAGCCCGGACAGGCTCCTG3990.5074270017295758No Hit
TTTAGGGGGGGTCTCTCGGGTGGAGTCTTCTGACTGCTGGTGGAGCAGGT3980.5061552548580731No Hit
TTTAGGGGGGAGTGCAGTGCTTTTAGGGGGAAAAAAAAAAAAAAAAAAAA3910.4972530267575542No Hit
TTTAGGGGGAGTGGGAGGGATGGAACCAACTTTTTCTGTATTTTGTATTG3800.48326381117102457No Hit
TTTAGGGGGACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCCCGAGCCGC3060.389154542679825No Hit
TTTAGGGGGGTTTTTGTTTTTTACGGTAGCCAAAACAAGCCCTGTGGAAA2830.359904364635263No Hit
TTTAGGGGGTCCAGTCCCGTGTCCCTGTGGCTGCGCAGGGCGGAGCGCCA2830.359904364635263No Hit
TTTAGGGGGGAGTGCAGTGCTTTTAGGGGAAAAAAAAAAAAAAAAAAAAA2730.34718689592023605No Hit
TTTAGGGGGGGACAGAATTCTCAGCAACGAATATGAAGACAAGTATGACC2640.3357411740767118No Hit
TTTAGGGGGGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAG2540.3230237053616848No Hit
TTTAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2410.30649099603214974No Hit
TTTAGGGGGGGCATTTCCAGGGTTCCCTCTCCCGGCTTCTGTGCTCCGCT2350.2988605148031336No Hit
TTTAGGGGGGAGCGAGGCGTCGCCACATCGAGGGCCCTTGTGGGGCTCAG2330.2963170210601282No Hit
TTTAGGGGGACTTGGACAACAACAAACTCCTCAGGGTGCCTGCTGGGCTG2270.288686539831112No Hit
TTTAGGGGGATGGGCGGGGCCCTGCGGTGTCCGCCCCTCAATAAAAGCAA2250.28614304608810665No Hit
TTTAGGGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACA2030.25816461491504733No Hit
TTTAGGGGGGGTTTTTCAGGACGCTTTGCAAGGGTAGCTGGGGTGAATTT2020.2568928680435446No Hit
TTTAGGGGGGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2000.2543493743005392No Hit
TTTAGGGGGATTTCAGTGGCTGCTACTCGGCGCTTCAGTCGCGGTCGCTT1970.2505341336860311No Hit
TTTAGGGGGAGTGCAGTGCTTTTAGGGGGAAAAAAAAAAAAAAAAAAAAA1940.24671889307152306No Hit
TTTAGGGGGGTCCTTCTGCTCAGAACCCTAGAGGACTCTAGGTGAAATTT1930.24544714620002034No Hit
TTTAGGGGGGGGCTGGAGAGATGGCTCAGCAGTTAAGAGCACTGATTGCT1900.24163190558551229No Hit
TTTAGGGGGCAGTTAACAGAACCCCCACAACCCTTCACCCCTTCACAGGC1880.23908841184250687No Hit
TTTAGGGGGCAGAGTGCAGTGCTTTTAGGGGGAAAAAAAAAAAAAAAAAA1830.23272967748499337No Hit
TTTAGGTGTCTGTAAAACAGGTGCCGAAGAAGCTGGAGTAACAGAAGTGA1820.2314579306134907No Hit
TTTAGGGGGGAGCCTTGTTTTGGACAGTATACCAGTTGCCTTTTATCCCA1810.23018618374198802No Hit
TTTAGGGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA1810.23018618374198802Illumina Single End Adapter 2 (97% over 34bp)
TTTAGGGGGAACCTTTCCTCTTTGTTCTGAGGGACACGGACACGGGGGCC1800.2289144368704853No Hit
TTTAGGGGGGCTCTTTCCTTTTGCGGCCATCGGTGGATCGCAGCCGCCAA1740.2212839556414691No Hit
TTTAGGGGGCAAGACCCACCTTCACGCCCCACCCCATTGTCCCTGTCACC1650.20983823379794483No Hit
TTTAGGGGGGAGGTTCCGGGAGGAACCGTGGGAGCCCAGCGTGTCTGTGC1650.20983823379794483No Hit
TTTAGGGGGCTCTTCGCCATCTTTACCCCGGCAGGCCGACATCTATCACC1640.2085664869264422No Hit
TTTAGGGGGACAGACTCTCCATGTACTGAGAGGGGAAGGAGGCGTGTTTT1620.20602299318343678No Hit
TTTAGGGGGGATTTCAGTGGCTGCTACTCGGCGCTTCAGTCGCGGTCGCT1610.20475124631193406No Hit
TTTAGGGGGGCGCCTCTTTTTTTCACCCCCAACCTGTTGCAAGTCTTTAA1560.1983925119544206No Hit
TTTAGGGGGGCTTCCTGTTCTCCACCATGGCGCAAGATCAAGGGGAAAAG1530.1945772713399125No Hit
TTTAGGGGGGCTTTCTGCGCTCTCGCTGGACAAGTGAGCAGGGACGGCCT1530.1945772713399125No Hit
TTTAGGGGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAA1510.19203377759690712Illumina Single End Adapter 2 (97% over 34bp)
TTTAGGGGGGAGTGCAGTGCAAAGGAGAAAGAGCTACATCCCAAGACACA1500.1907620307254044No Hit
TTTAGGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCC1490.18949028385390174No Hit
TTTAGGGGGACCCAGGTTTGGTTCCTCTAGGGGGGGGCCCCCACTGTGGA1480.18821853698239902No Hit
TTTAGGGGGAGTTTTCTGGTTGTTTGTGTCGGCAGCGGGCCAACTGTGGT1480.18821853698239902No Hit
TTTAGGGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT1430.18185980262488555No Hit
TTTAGGGGGTCTGTCCTCTGGCTTACTGCCTCATAGTTGTAAAATTGTTG1420.18058805575338285No Hit
TTTAGGGGGGGCTTCATCCCAAATTTATGCCATCCGACAGTCCATCTCAA1420.18058805575338285No Hit
TTTAGGGGAGGAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTG1410.17931630888188013No Hit
TTTAGGGGGGTCTGATGTCTTGTAATGGAACACATTTGTAAATATGCTCA1390.17677281513887474No Hit
TTTAGGGGGGTCTCTCGGGTGGAGTCTTCTGACTGCTGGTGGAGCAGGTC1380.17550106826737208No Hit
TTTAGGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTG1370.17422932139586936No Hit
TTTAGGGGGCAAAAAGTAAGTTTAGATTATAGCCAAAAGAGGGACAGCTC1370.17422932139586936No Hit
TTTAGGGGGGGCAGTTTGGCCAGCTGGATCAGCACCCTATTGATGGGTAC1360.17295757452436666No Hit
TTTAGGGGGGAGTGCAGTGCTTTTAGGGGGTATGAAATTCGGAAGTACTT1350.17168582765286397No Hit
TTTAGGGGTCTCCCTAGGAGATGGAGGCTCTCTGAGGACATGGGTGGGGT1340.17041408078136128No Hit
TTTAGGGGGAGTGCAGTGCTTTTAGGGGAAAAAAAAAAAAAAAAAAAAAA1340.17041408078136128No Hit
TTTAGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCG1340.17041408078136128No Hit
TTTAGGGGGGACTCCTGTCCCAGCCACCGTCAGCCACAGTCGCAGCCGGA1340.17041408078136128No Hit
TTTAGGGGGATGTACTGTTGCGTTGGAATCTACCTGCATCGTCCACCGAC1330.16914233390985858No Hit
TTTAGGGTCGGCATCAAAAGCAATATCAGCACCAACAGAAACAACCTGAT1330.16914233390985858No Hit
TTTAGGGGGCTTCTGGCAGGCTCGCCGCTGGAGGATGAGGCCACCCTAGG1320.1678705870383559No Hit
TTTAGGGGGGGGACATCCGTCAACCTGATACCAACCTGTGGATTCCAAGA1310.1665988401668532No Hit
TTTAGGGGGGTGAATACCGACAGCTACCATTACATTTATTATTTTACTTC1300.1653270932953505No Hit
TTTAGGAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA1300.1653270932953505Illumina Single End Adapter 2 (100% over 34bp)
TTTAGGGGGGAATGACCCCAAAGTTTGACTCCAACAACGTGGTCCTCATT1300.1653270932953505No Hit
TTTAGGGGGAATCAAAGGATGTAGTCCTGTGGTAGACCAGAGGAGTAACT1290.1640553464238478No Hit
TTTAGGGGGCGTCACGAAATCCTTCGCTTAATTGCCGCACTTTGATGGTC1290.1640553464238478No Hit
TTTAGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG1270.1615118526808424No Hit
TTTAGGGGGAAGCCCTGATGGCACGCAGGATCGGCTACTGCTGCCTCACG1250.158968358937837No Hit
TTTAGGGGGCGTGTGCTCTGCAGCAAGAGAAGCCACCGCAATGAGAAGCT1250.158968358937837No Hit
TTTAGGGGGGATCGTGGTGTGCCCAGCTCTTCCAAGGACTGCTGCGCTTC1240.1576966120663343No Hit
TTTAGGGGGGTGCAGTGCTTTTAGGGGGAAAAAAAAAAAAAAAAAAAAAA1220.15515311832332893No Hit
TTTAGGGGGGCTTCCACAGGAGGCCTACACGCCGCCGTCTGTGCCGCCGC1190.15133787770882084No Hit
TTTAGGGGGGCTCTTTCCAGCCAGCGCCGAGCGATGGGCATCTCTCGGGA1180.15006613083731812No Hit
TTTAGGGGGGTTGGTGGAGTATCTGCCCGTTCTGCTCGCCGATTGCGGCT1180.15006613083731812No Hit
TTTAGGGGGGTGCAGTGCTTTTAGGGGGATCATAGATGACATCAACAGTG1180.15006613083731812No Hit
TTTAGGGAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA1150.14625089022281004Illumina Single End Adapter 2 (100% over 34bp)
TTTAGGGGGCTTGGGAAAGCCAGGACACGAAAATCACGGTGACAATGACA1130.14370739647980466No Hit
TTTAGGGGGGATGGTTGCGCCGGCGTTGTCAGGATCGGCGCCTCCGGAGT1120.14243564960830196No Hit
TTTAGGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1120.14243564960830196No Hit
TTTAGGGGGGCTCTTTCTAAGCCGGCGATCCGTCAGGGTGAAGCCATGTT1110.14116390273679927No Hit
TTTAGGGGGGCTTCTTCCTCGGCGCTGCCTACGAGGTGGCTGCCATCTGT1100.13989215586529657No Hit
TTTAGGGGGAGTTTCTAATATGCTATACAATTTATGCACGCAGAAAGAAA1090.13862040899379385No Hit
TTTAGGGGGGCTCTTTCGAGTCTTGGCTCCAAGATGACCAAAAAAAGAAG1080.1373486621222912No Hit
TTTAGGGGGACCCAAGTGTGTGTACGCTTGCAGGATCTAGAACAATACAT1080.1373486621222912No Hit
TTTAGGGGGGTGTGTGCTCGCATCCAACTGTGATGAGCCCATGTATGTCA1070.13607691525078847No Hit
TTTAGGGGGATGTAATCACAACTTACTTTCTGCTTGTAGTGGCTTAAAAT1070.13607691525078847No Hit
TTTAGGGGGCTTTCTGCGCTCTCGCTGGACAAGTGAGCAGGGACGGCCTC1060.1348051683792858No Hit
TTTAGGGGGGAGGAAATGTGATCAGATCAGAGACACTTGATCGTTGGGAG1060.1348051683792858No Hit
TTTAGGGGGTAGCCGTGGGGTCCCAGCCCAACGCGACGAACCCCAGCGGC1050.13353342150778308No Hit
TTTAGGGGGGGCAATGCATAGCATTCCTTACCTTCAAAGTTTTCTCCATT1040.1322616746362804No Hit
TTTAGGGGGACGCAGAGTGCAGTGCCTTTAGGGGGCAACGCAGAGTGCAG1040.1322616746362804No Hit
TTTAGGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCAC1030.1309899277647777No Hit
TTTAGGGGGGGCTTTCTCTTGGGCCCGTGGCGCCGGCAAGATGGGCAAGT1030.1309899277647777No Hit
TTTAGGGGGTGAGTCTGACTCTGTTGCCAATGCTGGTCTTCAGCTGTTTC1020.129718180893275No Hit
TTTAGGGGGTAAAGAAGTCTCTGAAGCTGATGGGATCAAATGAAGGCGAA1020.129718180893275No Hit
TTTAGGGGGGAGACCCGCAGCGCGCTCCATCCTTTGCCCTTCAGTGCTGC1010.1284464340217723No Hit
TTTAGGGGGATGGATCTGAATAGAGAAATACTTTCAGATGATCATTAGCC1000.1271746871502696No Hit
TTTAGGGGGGAGTCCCGGAGCCGGTCCGCAGAGGGAAGGAGGCAAGATGG1000.1271746871502696No Hit
TTTAGGGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCG1000.1271746871502696No Hit
TTTAGGGGGGGATTTGAACTTTGCATCTGTGATTCCCGGCTGCTGGTTTG980.12463119340726421No Hit
TTTAGGGGGACCTACCCCAGCGGCTGCCAGCTGCGCGCTGCCAGCCTGCG970.12335944653576153No Hit
TTTAGGGGGGCACGCGTCCTCCTCGCTCTCCTCGTCCTCTTCCTCCTCCT970.12335944653576153No Hit
TTTAGGGGGCAGAGTGCAGTGCTTTTAGGGGAAAAAAAAAAAAAAAAAAA960.12208769966425882No Hit
TTTAGGGGGAGATCTGGGTAAAGGTGGCGCGTGTAAAGAAACTTGATTAC960.12208769966425882No Hit
TTTAGGGGTAGAGGCGGCATGACAAGAAACCGTGGCTCTGGAGGTTTTGG940.11954420592125344No Hit
TTTAGGGGGACGCAGAGTGCAGTGCTTTTAGGGGGGGCTCAGGTGGTCTC930.11827245904975074No Hit
TTTAGGGGAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAA930.11827245904975074Illumina Single End Adapter 2 (100% over 34bp)
TTTAGGGGAGGAACCAGGCTGCTTCAGCCGTGAACAACGCCGAAATGGCC930.11827245904975074No Hit
TTTAGGGGGGGGAGCCGAGCTGGAGCTGGAGCACCTCTCACACCCTCCTC920.11700071217824803No Hit
TTTAGGGGGGAGTGCTGCCTTTGCTCCGCACCATGAACCACACTTCTCAA910.11572896530674535No Hit
TTTAGGGGGATTCTTGTTTTGTTTTTTGGCTTTATTTATTTATTTATTTT910.11572896530674535No Hit
TTTAGGGGCTTTTTCCGGTGGAGGAGTCTGGAGACGACGATCAGAAATGG900.11445721843524265No Hit
TTTAGGGGGAATGAATTGATCAAATATTTCTTTCCAAGGAATGCAGTTAT900.11445721843524265No Hit
TTTAGGGGGGTTTGCCTTTCTTAGATCACCGTCTAAGCTCAGGTCCAGTC900.11445721843524265No Hit
TTTAGGGGGTTCCGGCGGTGACGACCTCCCTACGAGAACATGCCTCTCGC890.11318547156373994No Hit
TTTAGGGGGACCCTGTGTGCTGCTCTGTGGTGGCGGAAGAAGGGGCTGGC890.11318547156373994No Hit
TTTAGGGGGGTAGGCATCACAGTAGCGTGTACCTTTAATAATCCCAGCAC890.11318547156373994No Hit
TTTAGGGGGCTGTTTGCAGGCTTAACTCCGGCCGGGGTTCCTCGCTCGAG880.11191372469223726No Hit
TTTAGGGGGGAAGCGCTAGGGCGCCCTCCCGGAAGCGCGAGCCAGGCCGC880.11191372469223726No Hit
TTTAGGGGGGGGAGGGGAACCAGGGACAGAGAGAACTGGCCACTCTGAGT880.11191372469223726No Hit
TTTAGGGGGGAGCCGAGCCCATTGCGGGAGTAGCAGCAGCGGCCAGGCAG880.11191372469223726No Hit
TTTAGGGGGACTCTGCTACACTTAGTCTTTTTACTTTTGAGTTTTTTGTT880.11191372469223726No Hit
TTTAGGGGGATTGCCTGCAGCCATCTGCACACTGCACCTGCTGGCAGCTC870.11064197782073455No Hit
TTTAGGGGGGATCCCTCCAGCTACTTTGCAGCACTCTGTCGCCATGAGCA860.10937023094923187No Hit
TTTAGGGGGGCTTTCTCTTCGCCATCTTTACCCCGGCAGGCCGACATCTA850.10809848407772917No Hit
TTTAGGGGGTGCAGATGACGTTGTGTGCACCAGGGTCTACGTCCGAGAGT850.10809848407772917No Hit
TTTAGGGGGCTTTCCGGACCTGGCCGAGCAGGAGGCGCCATCATGGGTGT840.10682673720622649No Hit
TTTAGGGGGGTGCTTTTAGGGGGCAAGCCTTCATCACCGCTGCCAGTGGA830.10555499033472378No Hit
TTTAGGGGGCTCTCTTCCTGTTCTCCACCATGGCGCAAGATCAAGGGGAA830.10555499033472378No Hit
TTTAGGGGGGACTCGAAGTCGGGCGGCGGCTGAGGCTCAGCGCACAACGC830.10555499033472378No Hit
TTTAGGGGGCTCTTTCTCTTGGGCCCGTGGCGCCGGCAAGATGGGCAAGT830.10555499033472378No Hit
TTTAGGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGA820.1042832434632211No Hit
TTTAGGGGGGACATTCCTGCAGCCCTTCAGACCGCCAGAACTCTTCTGCC820.1042832434632211No Hit
TTTAGGGGTTGGCTCACACTGAATTCACCCCCACTGAGACTGATACATAC810.10301149659171839No Hit
TTTAGGGGCACCTATAAAGTTGTTTTGACACCATGCATGTGCTGTGGTGT800.10173974972021568No Hit
TTTAGGGGGGCTCTTTGCTGCCAGACCTCCGCCATCATGGGTCGCATGCA800.10173974972021568No Hit
TTTAGGGGGATGACTTTGGTTTTGAAGGAGACTTCTACAGGGCTGACCAG800.10173974972021568No Hit
TTTAGGGGGAAAGAGTGAAGCCCAGAAACAAAGCGATGAGCTTGCCCTGG800.10173974972021568No Hit
TTTAGGGGGAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAA790.10046800284871299Illumina Single End Adapter 2 (100% over 34bp)
TTTAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA790.10046800284871299No Hit
TTTAGGGGGCTTTTTGGCTCTCCGGTCGGCACCATGGCGGTCGGCAAGAA790.10046800284871299No Hit
TTTAGGGGGGCCTCCATCCCAGGACCAGCCCCTGGTGGAGGCCAACCACC790.10046800284871299No Hit
TTTAGGGGGAACCGCTCTCTGTTACTTGGTCACTGTTGTGACCCGCGTGG790.10046800284871299No Hit
TTTAGGGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTTTAGGGGGA790.10046800284871299No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTTGTA600.044.00000441
GGAGATT207.8117475E-444.00000444
TTGTCTC207.8117475E-444.00000444
ACAGCCA207.8117475E-444.00000419
ACAGTAT207.8117475E-444.00000424
CAAGAGG207.8117475E-444.00000424
TTGTCCC207.8117475E-444.00000437
GTATGAC302.4976307E-644.00000443
TTCACGC207.8117475E-444.00000421
ACGCTTT600.044.00000421
TTGCAGC302.4976307E-644.00000426
AACAACG207.8117475E-444.00000433
GTGTTCA408.143616E-944.00000427
ACGCCCC207.8117475E-444.00000424
CTGAGCG207.8117475E-444.00000415
TAAATAT408.143616E-944.00000439
TAGTGGC207.8117475E-444.00000437
CACATCG207.8117475E-444.00000424
TATACCA207.8117475E-444.00000428
CCAGCTA207.8117475E-444.00000417
Produced by FastQC (version 0.11.5)