FastQCFastQC Report
Fri 17 Jun 2016
SRR1527620_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527620_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences118136
Sequences flagged as poor quality0
Sequence length52
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9990.8456355387011579No Hit
TTTAGGGGGGGTCTCTCGGGTGGAGTCTTCTGACTGCTGGTGGAGCAGGTCT6170.5222794067853999No Hit
TTTAGGGGGCGGCGACGGTGGTGAGGGCCTTGAGCCCGGACAGGCTCCTGCC5980.5061962483916841No Hit
TTTAGGGGGAGTGGGAGGGATGGAACCAACTTTTTCTGTATTTTGTATTGTA5960.5045032843502404No Hit
TTTAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5910.500270874246631No Hit
TTTAGGGGGGAGTGCAGTGCTTTTAGGGGGAAAAAAAAAAAAAAAAAAAAAA5700.48249475181147156No Hit
TTTAGGGGGGTTTTTGTTTTTTACGGTAGCCAAAACAAGCCCTGTGGAAACT4840.4096972980293899No Hit
TTTAGGGGGTCCAGTCCCGTGTCCCTGTGGCTGCGCAGGGCGGAGCGCCAGG4590.38853524751134283No Hit
TTTAGGGGGACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCCCGAGCCGCCT4550.3851493194284553No Hit
TTTAGGGGGGAGTGCAGTGCTTTTAGGGGAAAAAAAAAAAAAAAAAAAAAAA4050.34282521839236135No Hit
TTTAGGGGGGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA3880.3284350240400894No Hit
TTTAGGGGGGGACAGAATTCTCAGCAACGAATATGAAGACAAGTATGACCTC3830.32420261393648No Hit
TTTAGGGGGGGCATTTCCAGGGTTCCCTCTCCCGGCTTCTGTGCTCCGCTCA3660.309812419584208No Hit
TTTAGGGGGACTTGGACAACAACAAACTCCTCAGGGTGCCTGCTGGGCTGGC3430.2903433331076048No Hit
TTTAGGGGGGAGCGAGGCGTCGCCACATCGAGGGCCCTTGTGGGGCTCAGCC3400.28780388704543913No Hit
TTTAGGGGGGCTCTTTCCTTTTGCGGCCATCGGTGGATCGCAGCCGCCAAAA3330.281878512900386No Hit
TTTAGGGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAAC3270.2767996207760547No Hit
TTTAGGGGGGGGCTGGAGAGATGGCTCAGCAGTTAAGAGCACTGATTGCTCT3250.275106656734611No Hit
TTTAGGGGGGAGCCTTGTTTTGGACAGTATACCAGTTGCCTTTTATCCCAAA3210.2717207286517235No Hit
TTTAGGGGGATTTCAGTGGCTGCTACTCGGCGCTTCAGTCGCGGTCGCTTCA3200.27087424663100157No Hit
TTTAGGGGGGTCCTTCTGCTCAGAACCCTAGAGGACTCTAGGTGAAATTTTA3100.2624094264237828No Hit
TTTAGGGGGGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2980.2522516421751202No Hit
TTTAGGGGGATGGGCGGGGCCCTGCGGTGTCCGCCCCTCAATAAAAGCAATT2930.2480192320715108No Hit
TTTAGGGGGCAGAGTGCAGTGCTTTTAGGGGGAAAAAAAAAAAAAAAAAAAA2920.24717275005078895No Hit
TTTAGGGGGGGTTTTTCAGGACGCTTTGCAAGGGTAGCTGGGGTGAATTTGC2910.24632626803006702No Hit
TTTAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2800.23701496580212636No Hit
TTTAGGGGGCAGTTAACAGAACCCCCACAACCCTTCACCCCTTCACAGGCAT2760.23362903771923885No Hit
TTTAGGGGGACAGACTCTCCATGTACTGAGAGGGGAAGGAGGCGTGTTTTGC2720.23024310963635133No Hit
TTTAGGGGGAGTGCAGTGCTTTTAGGGGGAAAAAAAAAAAAAAAAAAAAAAA2620.22177828942913252No Hit
TTTAGGGGGCTCTTCGCCATCTTTACCCCGGCAGGCCGACATCTATCACCAT2580.218392361346245No Hit
TTTAGGGGGACCCAGGTTTGGTTCCTCTAGGGGGGGGCCCCCACTGTGGAGA2540.2150064332633575No Hit
TTTAGGGGGGATTTCAGTGGCTGCTACTCGGCGCTTCAGTCGCGGTCGCTTC2520.21331346922191372No Hit
TTTAGGGGGGCTTCCTGTTCTCCACCATGGCGCAAGATCAAGGGGAAAAGGA2510.21246698720119184No Hit
TTTAGGGGGGAGGTTCCGGGAGGAACCGTGGGAGCCCAGCGTGTCTGTGCCG2440.2065416130561387No Hit
TTTAGGGGGAATCAAAGGATGTAGTCCTGTGGTAGACCAGAGGAGTAACTAG2370.20061623891108551No Hit
TTTAGGGGGAACCTTTCCTCTTTGTTCTGAGGGACACGGACACGGGGGCCCT2350.1989232748696418No Hit
TTTAGGGGGGTGAATACCGACAGCTACCATTACATTTATTATTTTACTTCTA2270.19215141870386673No Hit
TTTAGGGGGCAAGACCCACCTTCACGCCCCACCCCATTGTCCCTGTCACCCT2260.19130493668314486No Hit
TTTAGGGGGGCTTTCTGCGCTCTCGCTGGACAAGTGAGCAGGGACGGCCTCA2250.19045845466242298No Hit
TTTAGGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGG2250.19045845466242298No Hit
TTTAGGGGAGGAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAG2220.18791900860025734No Hit
TTTAGGGGGGAATGACCCCAAAGTTTGACTCCAACAACGTGGTCCTCATTGA2210.18707252657953544No Hit
TTTAGGGGGGCTCTTTCGAGTCTTGGCTCCAAGATGACCAAAAAAAGAAGAA2170.18368659849664792No Hit
TTTAGGGGGGGCTTCATCCCAAATTTATGCCATCCGACAGTCCATCTCAAAG2160.18284011647592607No Hit
TTTAGGGGGGTCTCTCGGGTGGAGTCTTCTGACTGCTGGTGGAGCAGGTCTC2160.18284011647592607No Hit
TTTAGGGGGAGTTTTCTGGTTGTTTGTGTCGGCAGCGGGCCAACTGTGGTTT2140.1811471524344823No Hit
TTTAGGGGGGGGACATCCGTCAACCTGATACCAACCTGTGGATTCCAAGATG2130.1803006704137604No Hit
TTTAGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT2110.17860770637231665No Hit
TTTAGGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCA2070.17522177828942914No Hit
TTTAGGGGGGGCAGTTTGGCCAGCTGGATCAGCACCCTATTGATGGGTACCT2020.17098936818581975No Hit
TTTAGGGGGGCGCCTCTTTTTTTCACCCCCAACCTGTTGCAAGTCTTTAATC2020.17098936818581975No Hit
TTTAGGGGGGAGTGCAGTGCAAAGGAGAAAGAGCTACATCCCAAGACACAAA2010.17014288616509785No Hit
TTTAGGGGGGCTCTTTCCAGCCAGCGCCGAGCGATGGGCATCTCTCGGGACA2000.16929640414437597No Hit
TTTAGGGGGTAGCCGTGGGGTCCCAGCCCAACGCGACGAACCCCAGCGGCAA1930.16337102999932282No Hit
TTTAGGGGGCTTTCTGCGCTCTCGCTGGACAAGTGAGCAGGGACGGCCTCAG1930.16337102999932282No Hit
TTTAGGGGGACGCAGAGTGCAGTGCCTTTAGGGGGCAACGCAGAGTGCAGTG1900.16083158393715719No Hit
TTTAGGGGGAGTTTCTAATATGCTATACAATTTATGCACGCAGAAAGAAATA1900.16083158393715719No Hit
TTTAGGGGGACGCAGAGTGCAGTGCTTTTAGGGGGGGCTCAGGTGGTCTCCT1890.1599851019164353No Hit
TTTAGGGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG1880.15913861989571343No Hit
TTTAGGGGGATGGATCTGAATAGAGAAATACTTTCAGATGATCATTAGCCCA1880.15913861989571343No Hit
TTTAGGGGGCTTCTGGCAGGCTCGCCGCTGGAGGATGAGGCCACCCTAGGCC1870.15829213787499152No Hit
TTTAGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGA1860.15744565585426967No Hit
TTTAGGGGGGCTCTTTCTAAGCCGGCGATCCGTCAGGGTGAAGCCATGTTCA1850.15659917383354777No Hit
TTTAGGGGGAAGCCCTGATGGCACGCAGGATCGGCTACTGCTGCCTCACGCT1850.15659917383354777No Hit
TTTAGGGGGCAAAAAGTAAGTTTAGATTATAGCCAAAAGAGGGACAGCTCTT1840.1557526918128259No Hit
TTTAGGGGGCGTGTGCTCTGCAGCAAGAGAAGCCACCGCAATGAGAAGCTTG1820.15405972777138213No Hit
TTTAGGGGGGTGTGTGCTCGCATCCAACTGTGATGAGCCCATGTATGTCAAG1820.15405972777138213No Hit
TTTAGGGGGGTTGGTGGAGTATCTGCCCGTTCTGCTCGCCGATTGCGGCTCT1820.15405972777138213No Hit
TTTAGGGGGGCACGCGTCCTCCTCGCTCTCCTCGTCCTCTTCCTCCTCCTCG1790.1515202817092165No Hit
TTTAGGGGGGATCGTGGTGTGCCCAGCTCTTCCAAGGACTGCTGCGCTTCGG1790.1515202817092165No Hit
TTTAGGGGGGCGTAATGGCTGGCCTGTTGAACAAGTCTGGAAAGAAATGCAT1790.1515202817092165No Hit
TTTAGGGGGCAGTGCTTTTAGGGGGGCCTTTTGTGACTGGCGGTGAACGAGT1760.14898083564705086No Hit
TTTAGGGGGTCTGTCCTCTGGCTTACTGCCTCATAGTTGTAAAATTGTTGCC1750.14813435362632899No Hit
TTTAGGGGGGCTTCCACAGGAGGCCTACACGCCGCCGTCTGTGCCGCCGCCA1740.1472878716056071No Hit
TTTAGGGGGATGTAATCACAACTTACTTTCTGCTTGTAGTGGCTTAAAATTA1720.14559490756416332No Hit
TTTAGGGGTCTCCCTAGGAGATGGAGGCTCTCTGAGGACATGGGTGGGGTTT1720.14559490756416332No Hit
TTTAGGGGGCGTCACGAAATCCTTCGCTTAATTGCCGCACTTTGATGGTCAG1710.14474842554344144No Hit
TTTAGGGGGACCCAAGTGTGTGTACGCTTGCAGGATCTAGAACAATACATCG1710.14474842554344144No Hit
TTTAGGGGGCTTGGGAAAGCCAGGACACGAAAATCACGGTGACAATGACATA1680.1422089794812758No Hit
TTTAGGGGGGACTCCTGTCCCAGCCACCGTCAGCCACAGTCGCAGCCGGACC1680.1422089794812758No Hit
TTTAGGGGGGTGCAGTGCTTTTAGGGGGATCATAGATGACATCAACAGTGGT1650.13966953341911018No Hit
TTTAGGGGGACCTACCCCAGCGGCTGCCAGCTGCGCGCTGCCAGCCTGCGCG1640.1388230513983883No Hit
TTTAGGGGGGAGTGCAGTGCTTTTAGGGGGTATGAAATTCGGAAGTACTTTG1630.13797656937766642No Hit
TTTAGGGGGGCCTCCATCCCAGGACCAGCCCCTGGTGGAGGCCAACCACCTG1630.13797656937766642No Hit
TTTAGGGGTAGAGGCGGCATGACAAGAAACCGTGGCTCTGGAGGTTTTGGTG1620.13713008735694454No Hit
TTTAGGGATCTTTGCAGTTGCTTTGAGTTTTATTTTTTATTATTTAAAACTG1610.13628360533622266No Hit
TTTAGGGGGGAGACCCGCAGCGCGCTCCATCCTTTGCCCTTCAGTGCTGCCT1610.13628360533622266No Hit
TTTAGGGGGCAACGCAGAGTGCAGTGCTTTTAGGGGGAAACCGGAATTTTAT1600.13543712331550078No Hit
TTTAGGGGGGTCTGATGTCTTGTAATGGAACACATTTGTAAATATGCTCACT1580.13374415927405703No Hit
TTTAGGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACAG1570.13289767725333515No Hit
TTTAGGGGGGAGTCCCGGAGCCGGTCCGCAGAGGGAAGGAGGCAAGATGGTG1560.13205119523261324No Hit
TTTAGGGGGAATGAATTGATCAAATATTTCTTTCCAAGGAATGCAGTTATTT1560.13205119523261324No Hit
TTTAGGGGGGCTTCTTCCTCGGCGCTGCCTACGAGGTGGCTGCCATCTGTTT1560.13205119523261324No Hit
TTTAGGGGGCTCTCTTCCTGTTCTCCACCATGGCGCAAGATCAAGGGGAAAA1550.1312047132118914No Hit
TTTAGGGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA1550.1312047132118914Illumina Single End Adapter 2 (97% over 34bp)
TTTAGGGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT1500.12697230310828198No Hit
TTTAGGTGTCTGTAAAACAGGTGCCGAAGAAGCTGGAGTAACAGAAGTGAGA1500.12697230310828198No Hit
TTTAGGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGG1490.1261258210875601No Hit
TTTAGGGGGGGATTTGAACTTTGCATCTGTGATTCCCGGCTGCTGGTTTGTC1480.12527933906683822No Hit
TTTAGGGTCGGCATCAAAAGCAATATCAGCACCAACAGAAACAACCTGATTA1470.12443285704611634No Hit
TTTAGGGGGAGTGCAGTGCTTTTAGGGGAAAAAAAAAAAAAAAAAAAAAAAA1450.12273989300467258No Hit
TTTAGGGGGTGCAGATGACGTTGTGTGCACCAGGGTCTACGTCCGAGAGTGA1440.12189341098395069No Hit
TTTAGGGGGTGAGTCTGACTCTGTTGCCAATGCTGGTCTTCAGCTGTTTCAG1430.12104692896322883No Hit
TTTAGGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1430.12104692896322883No Hit
TTTAGGGGAGGAACCAGGCTGCTTCAGCCGTGAACAACGCCGAAATGGCCAA1420.12020044694250694No Hit
TTTAGGGGGGGCAATGCATAGCATTCCTTACCTTCAAAGTTTTCTCCATTGG1410.11935396492178507No Hit
TTTAGGGGGTACTTGGGCCCCGCCTTGGGGGCGCAGGACCCTAGGGCTCCTC1390.11766100088034132No Hit
TTTAGGGGGATGTACTGTTGCGTTGGAATCTACCTGCATCGTCCACCGACCG1390.11766100088034132No Hit
TTTAGGGGGATTCTTGTTTTGTTTTTTGGCTTTATTTATTTATTTATTTTCT1370.11596803683889753No Hit
TTTAGGGGGCAGAGTGCAGTGCTTTTAGGGGAAAAAAAAAAAAAAAAAAAAA1370.11596803683889753No Hit
TTTAGGGGGGAGGAAATGTGATCAGATCAGAGACACTTGATCGTTGGGAGTC1360.11512155481817567No Hit
TTTAGGGGGGATGGTTGCGCCGGCGTTGTCAGGATCGGCGCCTCCGGAGTAC1360.11512155481817567No Hit
TTTAGGGGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA1350.11427507279745377Illumina Single End Adapter 2 (97% over 34bp)
TTTAGGGGGGTTTCAGAAGAAAGAGAGGGATCAGATAGGCCCAGGAACTAGA1350.11427507279745377No Hit
TTTAGGGGGATTGCCTGCAGCCATCTGCACACTGCACCTGCTGGCAGCTCCT1350.11427507279745377No Hit
TTTAGGGGGGAGCCGAGCCCATTGCGGGAGTAGCAGCAGCGGCCAGGCAGCA1340.11342859077673191No Hit
TTTAGGGGGGATCCCTCCAGCTACTTTGCAGCACTCTGTCGCCATGAGCACC1340.11342859077673191No Hit
TTTAGGGGGGTAGGCATCACAGTAGCGTGTACCTTTAATAATCCCAGCACTT1310.11088914471456626No Hit
TTTAGGGGGCTGTTTGCAGGCTTAACTCCGGCCGGGGTTCCTCGCTCGAGCT1310.11088914471456626No Hit
TTTAGGGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTAC1300.1100426626938444No Hit
TTTAGGGGGTTCCGGCGGTGACGACCTCCCTACGAGAACATGCCTCTCGCAA1290.1091961806731225No Hit
TTTAGGGGGGTTTGCCTTTCTTAGATCACCGTCTAAGCTCAGGTCCAGTCCC1290.1091961806731225No Hit
TTTAGGGGGGACTTCCAACGTTTGAGAGGAGGGCGGGGTGTCGCTCCCTTTC1280.10834969865240061No Hit
TTTAGGGGGGACTCGAAGTCGGGCGGCGGCTGAGGCTCAGCGCACAACGCAG1280.10834969865240061No Hit
TTTAGGGGGAGATCTGGGTAAAGGTGGCGCGTGTAAAGAAACTTGATTACAC1280.10834969865240061No Hit
TTTAGGGGGGAGTGCTGCCTTTGCTCCGCACCATGAACCACACTTCTCAAGC1270.10750321663167875No Hit
TTTAGGGGGCTTTCCGGACCTGGCCGAGCAGGAGGCGCCATCATGGGTGTTG1270.10750321663167875No Hit
TTTAGGGGGCTCTTTCTCTTGGGCCCGTGGCGCCGGCAAGATGGGCAAGTGT1270.10750321663167875No Hit
TTTAGGGGGGCTCTTTGCTGCCAGACCTCCGCCATCATGGGTCGCATGCACG1270.10750321663167875No Hit
TTTAGGGGGGGCGGCGGGTGTGCGAGGCGCGGGCTGTTGTGCTCCCGGCTCT1270.10750321663167875No Hit
TTTAGGGGGGACATTCCTGCAGCCCTTCAGACCGCCAGAACTCTTCTGCCGC1250.105810252590235No Hit
TTTAGGGGGACTCTGCTACACTTAGTCTTTTTACTTTTGAGTTTTTTGTTTT1240.1049637705695131No Hit
TTTAGGGGGGAGCACTTCCCCCCTACACAATGCTGCCTGCTGCCCTAACCTC1240.1049637705695131No Hit
TTTAGGGGGGGAGGATTGTTGATGATTGCAGGCGCATTATTAAGCGGATGGC1240.1049637705695131No Hit
TTTAGGGGGCTTTTTGGCTCTCCGGTCGGCACCATGGCGGTCGGCAAGAACA1220.10327080652806934No Hit
TTTAGGGGGGGCCGGTTGCAGCCAGCTGTGAGAGCTGCACTCCCGTCTCTGC1220.10327080652806934No Hit
TTTAGGGGGGCTTTCTCTTCGCCATCTTTACCCCGGCAGGCCGACATCTATC1220.10327080652806934No Hit
TTTAGGGGGGGCTTTCTCTTGGGCCCGTGGCGCCGGCAAGATGGGCAAGTGT1210.10242432450734748No Hit
TTTAGGGGGCCGTTTTAACATGGCAGCCTCCGCCATAAAGGGGCTAAGCGCA1210.10242432450734748No Hit
TTTAGGGGGGAAGCGCTAGGGCGCCCTCCCGGAAGCGCGAGCCAGGCCGCCA1210.10242432450734748No Hit
TTTAGGGGGTAAAGAAGTCTCTGAAGCTGATGGGATCAAATGAAGGCGAATT1200.10157784248662559No Hit
TTTAGGGGGGTGCAGTGCTTTTAGGGGGAAAAAAAAAAAAAAAAAAAAAAAA1190.1007313604659037No Hit
TTTAGGAGGAAGAAACAAGACCTTGATGAGAACGACATTCTGAGAGAGCTGG1190.1007313604659037No Hit
TTTAGGGGGACCCTGTGTGCTGCTCTGTGGTGGCGGAAGAAGGGGCTGGCCT1190.1007313604659037No Hit
TTTAGGGGGAGAACTGAGGAACCACGTAGTCACTCACTCCAGTGACCGGCCT1190.1007313604659037No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTTGTA551.8189894E-1246.041
CGACAGT253.3984386E-546.035
CGACAGC253.3984386E-546.018
CGTTCCC301.8471528E-646.020
TTATGCC253.3984386E-546.025
TGTGAGC206.288365E-446.032
CTACAAC206.288365E-446.038
CTCTGAT206.288365E-446.026
ACAGTAT405.5388227E-946.024
AACGTTC301.8471528E-646.018
TAGCGGG206.288365E-446.033
GCGGGCC253.3984386E-546.035
AAACCTT206.288365E-446.036
GAGGCAT650.046.09
CGTCAGG253.3984386E-546.031
TGTTGCA206.288365E-446.035
CTTCACG453.0559022E-1046.020
GTAGACC206.288365E-446.032
GGAGACT253.3984386E-546.027
TTTTGAC253.3984386E-546.017
Produced by FastQC (version 0.11.5)