FastQCFastQC Report
Fri 17 Jun 2016
SRR1527619_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527619_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences118780
Sequences flagged as poor quality0
Sequence length52
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9570.8056911938036707No Hit
TTTAGGGGGGGTCTCTCGGGTGGAGTCTTCTGACTGCTGGTGGAGCAGGTCT5840.49166526351237577No Hit
TTTAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5640.47482741202222595No Hit
TTTAGGGGGCGGCGACGGTGGTGAGGGCCTTGAGCCCGGACAGGCTCCTGCC5390.4537800976595387No Hit
TTTAGGGGGAGTGGGAGGGATGGAACCAACTTTTTCTGTATTTTGTATTGTA5080.42768142784980634No Hit
TTTAGGGGGGAGTGCAGTGCTTTTAGGGGGAAAAAAAAAAAAAAAAAAAAAA4980.4192625021047314No Hit
TTTAGGGGGACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCCCGAGCCGCCT4280.36033002188920693No Hit
TTTAGGGGGGTTTTTGTTTTTTACGGTAGCCAAAACAAGCCCTGTGGAAACT4130.34770163327159453No Hit
TTTAGGGGGTCCAGTCCCGTGTCCCTGTGGCTGCGCAGGGCGGAGCGCCAGG3970.3342313520794747No Hit
TTTAGGGGGGGCATTTCCAGGGTTCCCTCTCCCGGCTTCTGTGCTCCGCTCA3780.3182353931638323No Hit
TTTAGGGGGGGACAGAATTCTCAGCAACGAATATGAAGACAAGTATGACCTC3750.3157097154403098No Hit
TTTAGGGGGGAGTGCAGTGCTTTTAGGGGAAAAAAAAAAAAAAAAAAAAAAA3690.31065835999326485No Hit
TTTAGGGGGGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA3630.30560700454621986No Hit
TTTAGGGGGGAGCGAGGCGTCGCCACATCGAGGGCCCTTGTGGGGCTCAGCC3310.27866644216198017No Hit
TTTAGGGGGACTTGGACAACAACAAACTCCTCAGGGTGCCTGCTGGGCTGGC3220.2710894089914127No Hit
TTTAGGGGGGGGCTGGAGAGATGGCTCAGCAGTTAAGAGCACTGATTGCTCT3130.26351237582084525No Hit
TTTAGGGGGGTCCTTCTGCTCAGAACCCTAGAGGACTCTAGGTGAAATTTTA3130.26351237582084525No Hit
TTTAGGGGGGAGCCTTGTTTTGGACAGTATACCAGTTGCCTTTTATCCCAAA3100.2609866980973228No Hit
TTTAGGGGGGGTTTTTCAGGACGCTTTGCAAGGGTAGCTGGGGTGAATTTGC3070.2584610203738003No Hit
TTTAGGGGGATGGGCGGGGCCCTGCGGTGTCCGCCCCTCAATAAAAGCAATT2910.2449907391816804No Hit
TTTAGGGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAAC2890.24330695403266545No Hit
TTTAGGGGGGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2880.2424650614581579No Hit
TTTAGGGGGATTTCAGTGGCTGCTACTCGGCGCTTCAGTCGCGGTCGCTTCA2810.2365718134366055No Hit
TTTAGGGGGGCTCTTTCCTTTTGCGGCCATCGGTGGATCGCAGCCGCCAAAA2810.2365718134366055No Hit
TTTAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2750.23152045798956056No Hit
TTTAGGGGGCAGTTAACAGAACCCCCACAACCCTTCACCCCTTCACAGGCAT2710.22815288769153058No Hit
TTTAGGGGGACAGACTCTCCATGTACTGAGAGGGGAAGGAGGCGTGTTTTGC2660.2239434248189931No Hit
TTTAGGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGG2640.22225963966997808No Hit
TTTAGGGGGCTCTTCGCCATCTTTACCCCGGCAGGCCGACATCTATCACCAT2590.21805017679744063No Hit
TTTAGGGGGACCCAGGTTTGGTTCCTCTAGGGGGGGGCCCCCACTGTGGAGA2540.21384071392490317No Hit
TTTAGGGGGCAGAGTGCAGTGCTTTTAGGGGGAAAAAAAAAAAAAAAAAAAA2470.20794746590335075No Hit
TTTAGGGGGAACCTTTCCTCTTTGTTCTGAGGGACACGGACACGGGGGCCCT2390.2012123253072908No Hit
TTTAGGGGGGATTTCAGTGGCTGCTACTCGGCGCTTCAGTCGCGGTCGCTTC2380.2003704327327833No Hit
TTTAGGGGGGCTTTCTGCGCTCTCGCTGGACAAGTGAGCAGGGACGGCCTCA2360.1986866475837683No Hit
TTTAGGGGGAGTGCAGTGCTTTTAGGGGGAAAAAAAAAAAAAAAAAAAAAAA2320.19531907728573836No Hit
TTTAGGGGGGCTTCCTGTTCTCCACCATGGCGCAAGATCAAGGGGAAAAGGA2310.19447718471123085No Hit
TTTAGGGGGGAGGTTCCGGGAGGAACCGTGGGAGCCCAGCGTGTCTGTGCCG2280.19195150698770835No Hit
TTTAGGGGGGCTCTTTCCAGCCAGCGCCGAGCGATGGGCATCTCTCGGGACA2190.18437447381714092No Hit
TTTAGGGGGAGTTTTCTGGTTGTTTGTGTCGGCAGCGGGCCAACTGTGGTTT2160.18184879609361845No Hit
TTTAGGGGGGGCAGTTTGGCCAGCTGGATCAGCACCCTATTGATGGGTACCT2150.18100690351911097No Hit
TTTAGGGGGGGGACATCCGTCAACCTGATACCAACCTGTGGATTCCAAGATG2140.18016501094460347No Hit
TTTAGGGGGCAAGACCCACCTTCACGCCCCACCCCATTGTCCCTGTCACCCT2130.17932311837009599No Hit
TTTAGGGGGGTCTCTCGGGTGGAGTCTTCTGACTGCTGGTGGAGCAGGTCTC2110.177639333221081No Hit
TTTAGGGGAGGAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAG2080.17511365549755853No Hit
TTTAGGGGGAATCAAAGGATGTAGTCCTGTGGTAGACCAGAGGAGTAACTAG2080.17511365549755853No Hit
TTTAGGGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG2070.17427176292305102No Hit
TTTAGGGGGGAATGACCCCAAAGTTTGACTCCAACAACGTGGTCCTCATTGA2050.17258797777403603No Hit
TTTAGGGGGATGTAATCACAACTTACTTTCTGCTTGTAGTGGCTTAAAATTA2040.17174608519952853No Hit
TTTAGGGGGAGTTTCTAATATGCTATACAATTTATGCACGCAGAAAGAAATA2000.16837851490149855No Hit
TTTAGGGGGGATCGTGGTGTGCCCAGCTCTTCCAAGGACTGCTGCGCTTCGG1980.1666947297524836No Hit
TTTAGGGGGCTTTCTGCGCTCTCGCTGGACAAGTGAGCAGGGACGGCCTCAG1980.1666947297524836No Hit
TTTAGGGGTCTCCCTAGGAGATGGAGGCTCTCTGAGGACATGGGTGGGGTTT1970.16585283717797608No Hit
TTTAGGGGGGACTCCTGTCCCAGCCACCGTCAGCCACAGTCGCAGCCGGACC1960.1650109446034686No Hit
TTTAGGGGGGCGCCTCTTTTTTTCACCCCCAACCTGTTGCAAGTCTTTAATC1950.1641690520289611No Hit
TTTAGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT1940.1633271594544536No Hit
TTTAGGGGGGCGTAATGGCTGGCCTGTTGAACAAGTCTGGAAAGAAATGCAT1940.1633271594544536No Hit
TTTAGGGGGACGCAGAGTGCAGTGCTTTTAGGGGGGGCTCAGGTGGTCTCCT1900.15995958915642364No Hit
TTTAGGGGGGAGTGCAGTGCAAAGGAGAAAGAGCTACATCCCAAGACACAAA1880.15827580400740865No Hit
TTTAGGGGGCAAAAAGTAAGTTTAGATTATAGCCAAAAGAGGGACAGCTCTT1880.15827580400740865No Hit
TTTAGGGGGGCTCTTTCGAGTCTTGGCTCCAAGATGACCAAAAAAAGAAGAA1860.15659201885839366No Hit
TTTAGGGGGTCTGTCCTCTGGCTTACTGCCTCATAGTTGTAAAATTGTTGCC1830.1540663411348712No Hit
TTTAGGGGGAAGCCCTGATGGCACGCAGGATCGGCTACTGCTGCCTCACGCT1830.1540663411348712No Hit
TTTAGGGGGGTGAATACCGACAGCTACCATTACATTTATTATTTTACTTCTA1790.15069877083684122No Hit
TTTAGGGGGCGTGTGCTCTGCAGCAAGAGAAGCCACCGCAATGAGAAGCTTG1780.14985687826233374No Hit
TTTAGGGGGGGCTTCATCCCAAATTTATGCCATCCGACAGTCCATCTCAAAG1770.14901498568782623No Hit
TTTAGGGGGGAGACCCGCAGCGCGCTCCATCCTTTGCCCTTCAGTGCTGCCT1770.14901498568782623No Hit
TTTAGGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCA1730.14564741538979625No Hit
TTTAGGGGGCGTCACGAAATCCTTCGCTTAATTGCCGCACTTTGATGGTCAG1710.14396363024078127No Hit
TTTAGGGGGGTGCAGTGCTTTTAGGGGGATCATAGATGACATCAACAGTGGT1700.14312173766627379No Hit
TTTAGGGGGGAGTCCCGGAGCCGGTCCGCAGAGGGAAGGAGGCAAGATGGTG1690.14227984509176628No Hit
TTTAGGGGGACCTACCCCAGCGGCTGCCAGCTGCGCGCTGCCAGCCTGCGCG1690.14227984509176628No Hit
TTTAGGGGGTAGCCGTGGGGTCCCAGCCCAACGCGACGAACCCCAGCGGCAA1670.1405960599427513No Hit
TTTAGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGA1670.1405960599427513No Hit
TTTAGGGGGAGTGCAGTGCTTTTAGGGGAAAAAAAAAAAAAAAAAAAAAAAA1660.1397541673682438No Hit
TTTAGGGGGGCTTCCACAGGAGGCCTACACGCCGCCGTCTGTGCCGCCGCCA1630.13722848964472134No Hit
TTTAGGGGGGCTCTTTCTAAGCCGGCGATCCGTCAGGGTGAAGCCATGTTCA1610.13554470449570635No Hit
TTTAGGGGGACGCAGAGTGCAGTGCCTTTAGGGGGCAACGCAGAGTGCAGTG1610.13554470449570635No Hit
TTTAGGGGGGCTTCTTCCTCGGCGCTGCCTACGAGGTGGCTGCCATCTGTTT1570.13217713419767638No Hit
TTTAGGGGGCAACGCAGAGTGCAGTGCTTTTAGGGGGAAACCGGAATTTTAT1570.13217713419767638No Hit
TTTAGGGGGGCACGCGTCCTCCTCGCTCTCCTCGTCCTCTTCCTCCTCCTCG1560.1313352416231689No Hit
TTTAGGGGGATTGCCTGCAGCCATCTGCACACTGCACCTGCTGGCAGCTCCT1560.1313352416231689No Hit
TTTAGGGGGATGGATCTGAATAGAGAAATACTTTCAGATGATCATTAGCCCA1540.1296514564741539No Hit
TTTAGGGGGTGCAGATGACGTTGTGTGCACCAGGGTCTACGTCCGAGAGTGA1530.1288095638996464No Hit
TTTAGGGGGGGCAATGCATAGCATTCCTTACCTTCAAAGTTTTCTCCATTGG1510.1271257787506314No Hit
TTTAGGGGGGGATTTGAACTTTGCATCTGTGATTCCCGGCTGCTGGTTTGTC1510.1271257787506314No Hit
TTTAGGGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA1510.1271257787506314Illumina Single End Adapter 2 (97% over 34bp)
TTTAGGGGGGAGTGCAGTGCTTTTAGGGGGTATGAAATTCGGAAGTACTTTG1500.12628388617612393No Hit
TTTAGGGGGCTTGGGAAAGCCAGGACACGAAAATCACGGTGACAATGACATA1490.12544199360161645No Hit
TTTAGGGGGGTGTGTGCTCGCATCCAACTGTGATGAGCCCATGTATGTCAAG1490.12544199360161645No Hit
TTTAGGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGG1480.12460010102710893No Hit
TTTAGGGGGTTCCGGCGGTGACGACCTCCCTACGAGAACATGCCTCTCGCAA1440.12123253072907896No Hit
TTTAGGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACAG1420.119548745580064No Hit
TTTAGGGGGACCCAAGTGTGTGTACGCTTGCAGGATCTAGAACAATACATCG1420.119548745580064No Hit
TTTAGGGGGGTCTGATGTCTTGTAATGGAACACATTTGTAAATATGCTCACT1410.11870685300555649No Hit
TTTAGGGGGCAGTGCTTTTAGGGGGGCCTTTTGTGACTGGCGGTGAACGAGT1400.117864960431049No Hit
TTTAGGGGGACTCTGCTACACTTAGTCTTTTTACTTTTGAGTTTTTTGTTTT1400.117864960431049No Hit
TTTAGGGGTGCTCTCGGGTGGAGTCTTCTGACTGCTGGTGGAGCAGGTCTCA1390.1170230678565415No Hit
TTTAGGGGGTGAGTCTGACTCTGTTGCCAATGCTGGTCTTCAGCTGTTTCAG1390.1170230678565415No Hit
TTTAGGGGGCTTCTGGCAGGCTCGCCGCTGGAGGATGAGGCCACCCTAGGCC1390.1170230678565415No Hit
TTTAGGGGGTAAAGAAGTCTCTGAAGCTGATGGGATCAAATGAAGGCGAATT1390.1170230678565415No Hit
TTTAGGGGGGTTGGTGGAGTATCTGCCCGTTCTGCTCGCCGATTGCGGCTCT1390.1170230678565415No Hit
TTTAGGGGGAATGAATTGATCAAATATTTCTTTCCAAGGAATGCAGTTATTT1380.11618117528203402No Hit
TTTAGGGGGGGCTCAGTGGATGACCACTTTGCCAAAGCACTAGGTGACACAT1370.11533928270752651No Hit
TTTAGGGGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA1360.11449739013301903Illumina Single End Adapter 2 (97% over 34bp)
TTTAGGGGGATGTACTGTTGCGTTGGAATCTACCTGCATCGTCCACCGACCG1360.11449739013301903No Hit
TTTAGGGGGGGCTTTCTCTTGGGCCCGTGGCGCCGGCAAGATGGGCAAGTGT1350.11365549755851154No Hit
TTTAGGGGGGAGGAAATGTGATCAGATCAGAGACACTTGATCGTTGGGAGTC1350.11365549755851154No Hit
TTTAGGGGTTGGCTCACACTGAATTCACCCCCACTGAGACTGATACATACGC1340.11281360498400404No Hit
TTTAGGTGTCTGTAAAACAGGTGCCGAAGAAGCTGGAGTAACAGAAGTGAGA1330.11197171240949655No Hit
TTTAGGGGGGATGGTTGCGCCGGCGTTGTCAGGATCGGCGCCTCCGGAGTAC1330.11197171240949655No Hit
TTTAGGGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT1310.11028792726048156No Hit
TTTAGGGGAGGAACCAGGCTGCTTCAGCCGTGAACAACGCCGAAATGGCCAA1300.10944603468597405No Hit
TTTAGGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1300.10944603468597405No Hit
TTTAGGGGGAGATCTGGGTAAAGGTGGCGCGTGTAAAGAAACTTGATTACAC1300.10944603468597405No Hit
TTTAGGGGGGCCTCCATCCCAGGACCAGCCCCTGGTGGAGGCCAACCACCTG1300.10944603468597405No Hit
TTTAGGGGTAGAGGCGGCATGACAAGAAACCGTGGCTCTGGAGGTTTTGGTG1300.10944603468597405No Hit
TTTAGGGGGCTTTTTGGCTCTCCGGTCGGCACCATGGCGGTCGGCAAGAACA1290.10860414211146657No Hit
TTTAGGGGGGAGCCGAGCCCATTGCGGGAGTAGCAGCAGCGGCCAGGCAGCA1290.10860414211146657No Hit
TTTAGGGGGCTCTCTTCCTGTTCTCCACCATGGCGCAAGATCAAGGGGAAAA1280.10776224953695909No Hit
TTTAGGGGGGAGTGCTGCCTTTGCTCCGCACCATGAACCACACTTCTCAAGC1270.10692035696245158No Hit
TTTAGGGGGCTCTTTCTCTTGGGCCCGTGGCGCCGGCAAGATGGGCAAGTGT1270.10692035696245158No Hit
TTTAGGGATCTTTGCAGTTGCTTTGAGTTTTATTTTTTATTATTTAAAACTG1260.1060784643879441No Hit
TTTAGGGGCTTTTTCCGGTGGAGGAGTCTGGAGACGACGATCAGAAATGGCG1260.1060784643879441No Hit
TTTAGGGGGGTGCAGTGCTTTTAGGGGGAAAAAAAAAAAAAAAAAAAAAAAA1260.1060784643879441No Hit
TTTAGGGGGTGCTTTTCCCTTTCTGCCACCGCCATGCCATCCAGACTGAGGA1260.1060784643879441No Hit
TTTAGGGGGGCTCTGCTGCTACCAGGAGGACTCAACATGTTGGGAACACTGT1240.10439467923892912No Hit
TTTAGGGGGGGCCGGTTGCAGCCAGCTGTGAGAGCTGCACTCCCGTCTCTGC1230.10355278666442164No Hit
TTTAGGGGTGGTGCCTCTTTGAAATGGCATCATAGCACACATTGTCACAGAA1210.10186900151540665No Hit
TTTAGGGGGGCTCTTTGCTGCCAGACCTCCGCCATCATGGGTCGCATGCACG1210.10186900151540665No Hit
TTTAGGGGGGCTTTCTCTTCGCCATCTTTACCCCGGCAGGCCGACATCTATC1200.10102710894089914No Hit
TTTAGGGGGACCCTGTGTGCTGCTCTGTGGTGGCGGAAGAAGGGGCTGGCCT1200.10102710894089914No Hit
TTTAGGGGGGTGCTTTTAGGGGGCAAGCCTTCATCACCGCTGCCAGTGGAGG1190.10018521636639165No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAGTAT351.0092481E-746.00000424
TGTTGCA351.0092481E-746.00000435
ACACGGG351.0092481E-746.00000440
GAGAAAT351.0092481E-746.00000423
TCCTTAC351.0092481E-746.00000425
AACAAGT351.0092481E-746.00000430
CCGGAAT351.0092481E-746.00000441
ACTGATT750.046.00000441
TTACCTT351.0092481E-746.00000428
GACAGTA351.0092481E-746.00000423
TGCGCCG351.0092481E-746.00000416
GCGACGG700.046.00000412
CAGTATA351.0092481E-746.00000425
GGTCCCA351.0092481E-746.00000419
TCGGCAG351.0092481E-746.00000429
ATGGTTG351.0092481E-746.00000411
TTTCACC351.0092481E-746.00000421
GGCGACG700.046.00000411
TAATGGC351.0092481E-746.00000413
CTTAGCG351.0092481E-746.00000414
Produced by FastQC (version 0.11.5)