FastQCFastQC Report
Fri 17 Jun 2016
SRR1527618_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527618_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3043
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTTTCGGGGTGGCTCATGCCTATAATCCCAGCACTTTGGGAGGCCAAGG2688.807098258297733No Hit
CCTTTCCTTTATCAGCGGCAGACTTGCCACCAAGTCCAACCAAATCAAGC2106.90108445612882No Hit
CCTTTCTTTTTGGGGTAATTATACTCATCGCGAATATCCTTAAGAGGGCG2096.868222149194874No Hit
CCTTTCGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCC1775.816628327308577No Hit
CCTTTCGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAA1364.4692737430167595No Hit
CCTTTCCCATCTTGGCTTCCTTGCTGGTCAGATTGGTCGTCTTATTACCA1364.4692737430167595No Hit
CCTTTCGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCG1274.173512980611239No Hit
CCTTTCGCCATCAACTAACGATTCTGTCAAAAACTGACGCGTTGGATGAG1254.107788366743345No Hit
CCTTTCGGGGACAGGATCTGGAATCCATCTGTCGTTGATCTTTCACGCTG1103.6148537627341435No Hit
CCTTTCGGAGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAA862.8261583963194217No Hit
CCTTTCGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGC752.464673020046007No Hit
CCTTTCTGGGGATGGCACTGTGGAACTCTTCTTGAGATAATATTTAATGC682.2346368715083798No Hit
CCTTTCGGGGGAATTCGTGGAGAAAGAAATGGTTCGTCTGGCAGCATTTG551.8074268813670717No Hit
CCTTTCGGGGGGCGCGACTGCGTGAACGCGACATTACCCTCACTTTTGCC491.6102530397633912No Hit
CCTTTCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCCTTTCGGGA230.7558330594807755No Hit
CCTTTCTGGAGTGTCTGAAGACAGCGACAGTGTACTTACATATAATAAAT190.6243838317449885No Hit
CCTTTCGGGCAGTATAGCGACCAGTTCACGCGGAAGGGATATAGTCGAAA120.3943476832073612No Hit
CCTTTCGGGGGGAATTCGTGGAGAAAGAAATGGCTCCTCTGGCAGCATTT120.3943476832073612No Hit
CCTTTCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA120.3943476832073612No Hit
CCTTTCGGGCAGGCTCCTTTCGGGGTATCAACGCAGACTCGACTTTTTTT120.3943476832073612No Hit
CCTTTCGGGGACAGGATCTGGAATCCATCTGTCGTTGATCCTTCACGCTG100.3286230693394676No Hit
CCTTTCGGGTTAGCGCCAATTCACGGGGAAAGGGATATAGTCAAAAAAAA100.3286230693394676No Hit
CCTTTCGGGTCACAAAACCACACAAACCAGAACCCAAAAAAAAAAAAAAA90.29576076240552085No Hit
CCTTTCGGGAAGCAGTGGTATCAACGCAGATCGGAAGAGCTCGTATGCCG90.29576076240552085Illumina Single End Adapter 2 (100% over 23bp)
CCTTTCGGGCACGCTAGATCTTTTATTCTATACTTAAAAAAAAAAAAAAA80.26289845547157414No Hit
CCTTTCGGGCGTGCGAAAAAACATGATGATTGCAGTAAAAAAAAAAAAAA80.26289845547157414No Hit
CCTTTCGGGGGGGAATTCGTGGAGAAAGAAATGGCTCCTCTGGCAGCAAT70.23003614853762733No Hit
CCTTTCTCCACACTGTATCCAAGTTGCCTCCCAGAGGAGCCACCAGTTCT70.23003614853762733No Hit
CCTTTCGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTT70.23003614853762733No Hit
CCTTTCAATTTTCCTTAGCTTGTCTGAGATACTGACCTGTACATTTTGAA60.1971738416036806No Hit
CCTTTCGGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCAAT50.1643115346697338No Hit
CCTTTCGGGGTGGCTCATGCCTATAATCCCAGCACTTTGGGAGGACAAGG50.1643115346697338No Hit
CCTTTCGGGGTGTTGATATTTAAACAGATAGAACAATACAGGAAAAAAAA50.1643115346697338No Hit
CCTTTCGGGGTGGTATCAACGCAGTGTGCTCCTTTCGGGAAAAAAAAAAA50.1643115346697338No Hit
CCTTTCGGGCGCTAGATCTTTTATTCTATACTTGAAAAAAAAAAAAAAAA50.1643115346697338No Hit
CCTTTCGGGAATCCGGCAGGCACGACGACCGTTTGCCCTTCGCTGAAGGC50.1643115346697338No Hit
CCTTTCGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA50.1643115346697338No Hit
CCTTTCTCTTTTTCTGGAGGGCTCCTAGAGCCGGCGGATCCCGCGTCGCT50.1643115346697338No Hit
CCTTTCCTCTCTGACCTCCTGTGCCCTAAGGTCTTGGTTTTGTTGATTAT50.1643115346697338No Hit
CCTTTCGGTTATTTGGCTTGTCTTTTCCTGCACCCTCAACTGAGTGGCTA40.13144922773578707No Hit
CCTTTCTTTTTGGGGTAATTATACTAATCGCGAATATCCTTAAGAGGGCG40.13144922773578707No Hit
CCTTTCGGGAGGCTAGATCTTTTATTCTATACTCAAAAAAAAAAAAAAAA40.13144922773578707No Hit
CCTTTCGGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATT40.13144922773578707No Hit
CCTTTCGGGGGAATTCGTGGAGAAAGAAATGGCTCCTCTGGCAGCATTTG40.13144922773578707No Hit
CCTTTCGGGAAGCAGTGATATCACGCAGGGGAAAAAAAAAAAAAAAAAAA40.13144922773578707No Hit
CCTTTCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.13144922773578707No Hit
CCTTTCGGGAAGCAGTGGTATCAACGCAGAGTCCAGTGCTCCTTTCGGGA40.13144922773578707No Hit
CCTTTCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCCTATCGGGA40.13144922773578707No Hit
CCTTTCTTTTTGGGGTAATTATACTCATCGCGAATATCCTTAAGAGAGCG40.13144922773578707No Hit
CCTTTCTTTTTGGGGTAATTATACTCATCGCGAATATCCTTAAGAGCGCG40.13144922773578707No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGAAT301.7835682E-644.00000429
CGGGGGG404.5401976E-944.0000046
CGGGGGA404.5401976E-944.0000046
AGAGGGC301.7835682E-644.00000443
AAGAGGG301.7835682E-644.00000442
GCGAATA301.7835682E-644.00000430
TCGCGAA301.7835682E-644.00000428
TAAGAGG301.7835682E-644.00000441
CCTTTCT404.5401976E-944.0000041
TCGTCTG206.665984E-444.00000434
CCTTTCC650.044.0000041
ATCGCGA301.7835682E-644.00000427
TCATCGC301.7835682E-644.00000425
GAGGGCG301.7835682E-644.00000444
GCATTTG206.665984E-444.00000444
CTCATCG301.7835682E-644.00000424
TTCGGGG1200.044.0000044
TTCGGGA301.7835682E-644.0000044
CGTCTGG206.665984E-444.00000435
TTTCGGG1600.044.0000043
Produced by FastQC (version 0.11.5)