FastQCFastQC Report
Fri 17 Jun 2016
SRR1527617_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527617_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2565
Sequences flagged as poor quality0
Sequence length52
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTTTCGGGGTGGCTCATGCCTATAATCCCAGCACTTTGGGAGGCCAAGGAG2479.62962962962963No Hit
CCTTTCCTTTATCAGCGGCAGACTTGCCACCAAGTCCAACCAAATCAAGCAA1807.017543859649122No Hit
CCTTTCGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGG1676.510721247563353No Hit
CCTTTCTTTTTGGGGTAATTATACTCATCGCGAATATCCTTAAGAGGGCGTT1254.8732943469785575No Hit
CCTTTCGCCATCAACTAACGATTCTGTCAAAAACTGACGCGTTGGATGAGGA1154.483430799220272No Hit
CCTTTCCCATCTTGGCTTCCTTGCTGGTCAGATTGGTCGTCTTATTACCATT1094.249512670565302No Hit
CCTTTCGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACA1064.132553606237817No Hit
CCTTTCGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGA1034.015594541910331No Hit
CCTTTCGGAGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACA1013.9376218323586745No Hit
CCTTTCGGGGACAGGATCTGGAATCCATCTGTCGTTGATCTTTCACGCTGTC963.7426900584795324No Hit
CCTTTCGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCG773.0019493177387915No Hit
CCTTTCGGTTATTTGGCTTGTCTTTTCCTGCACCCTCAACTGAGTGGCTAAT501.949317738791423No Hit
CCTTTCTGGGGATGGCACTGTGGAACTCTTCTTGAGATAATATTTAATGCTT411.5984405458089668No Hit
CCTTTCGGGGGAATTCGTGGAGAAAGAAATGGTTCGTCTGGCAGCATTTGAT391.5204678362573099No Hit
CCTTTCGGGGGGCGCGACTGCGTGAACGCGACATTACCCTCACTTTTGCCAC371.442495126705653No Hit
CCTTTCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCCTTTCGGGAAG261.01364522417154No Hit
CCTTTCTGGAGTGTCTGAAGACAGCGACAGTGTACTTACATATAATAAATAA170.6627680311890838No Hit
CCTTTCGGGCAGTATAGCGACCAGTTCACGCGGAAGGGATATAGTCGAAAAA130.50682261208577No Hit
CCTTTCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCCTTTCGGGGAG120.46783625730994155No Hit
CCTTTCAATTTTCCTTAGCTTGTCTGAGATACTGACCTGTACATTTTGAACA120.46783625730994155No Hit
CCTTTCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA100.3898635477582846No Hit
CCTTTCGGGTTAGCGCCAATTCACGGGGAAAGGGATATAGTCAAAAAAAAAA80.31189083820662766No Hit
CCTTTCGGGGTGGTATCAACGCAGTGTGCTCCTTTCGGGAAAAAAAAAAAAA80.31189083820662766No Hit
CCTTTCTCCACACTGTATCCAAGTTGCCTCCCAGAGGAGCCACCAGTTCTTA80.31189083820662766No Hit
CCTTTCGGGTCACAAAACCACACAAACCAGAACCCAAAAAAAAAAAAAAAAA80.31189083820662766No Hit
CCTTTCGGGAAGCAGTGATATCACGCAGGGGAAAAAAAAAAAAAAAAAAAAA70.27290448343079926No Hit
CCTTTCGGGGACAGGATCTGGAATCCATCTGTCGTTGATCCTTCACGCTGTC70.27290448343079926No Hit
CCTTTCGGGCACGCTAGATCTTTTATTCTATACTTAAAAAAAAAAAAAAAAA70.27290448343079926No Hit
CCTTTCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCCTTTCGGGCAG60.23391812865497078No Hit
CCTTTCGGGGGGAATTCGTGGAGAAAGAAATGGCTCCTCTGGCCGCATTTTA60.23391812865497078No Hit
CCTTTCGGGAAAGCAACAAAGCGCACCAAAAAAAAAAAAAAAAAAAAAAAAA60.23391812865497078No Hit
CCTTTCGGGAAGCAGTGGTATCAACGCAGATCGGAAGAGCTCGTATGCCGTC50.1949317738791423Illumina Single End Adapter 2 (100% over 25bp)
CCTTTCGGGCGCTAGATCTTTTATTCTATACTTGAAAAAAAAAAAAAAAAAA50.1949317738791423No Hit
CCTTTCGGGGGGAATTCGTGGAGAAAGAAATGGCTCCTCTGGCAGCATTTTA50.1949317738791423No Hit
CCTTTCGGGCAGGCTCCTTTCGGGGTATCAACGCAGACTCGACTTTTTTTTT50.1949317738791423No Hit
CCTTTCGGGGGGAATTCGTGGAGAAAGAAATGGCTCCTCTGGCAGCATTTGA50.1949317738791423No Hit
CCTTTCGGGGGGGAATTCGTGGAGAAAGAAATGGTAATGTCGCGTTCACGCA50.1949317738791423No Hit
CCTTTCGGGGGGGAATTCGTGGAGAAAGAAATGGCTCCTCTGGCAGCATTTT50.1949317738791423No Hit
CCTTTCGGTTATTTGGCTTGTCTTTTCCTGCACCCTCAACTGAGGGGCTAAT50.1949317738791423No Hit
CCTTTCGGGAAGCAGTGGTATCAACGCAGAGTGCAGATCGGAAGAGCTCGTA40.15594541910331383No Hit
CCTTTCTCTTTTTCTGGAGGGCTCCTAGAGCCGGCGGATCCCGCGTCGCTCA40.15594541910331383No Hit
CCTTTCGGGATGAACAAAAAAGACCTCCAAAAAAAAAAAAAAAAAAAAAAAA40.15594541910331383No Hit
CCTTTCGGGAAGCAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAA40.15594541910331383Illumina Single End Adapter 2 (100% over 34bp)
CCTTTCGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.15594541910331383No Hit
CCTTTCTCTTCTATGCCAGTCTTTTCTCGGCTGTCATCTGAATCATCTGCTG40.15594541910331383No Hit
CCTTTCGGGGGGGAATTCGTGGAGAAAGAAATGGCTCCTCTGGCCGCATTTT40.15594541910331383No Hit
CCTTTCGGTTATTTGGCTTGTCTTTTCCTGCACCCTCCACTGAGTGGCTAAT40.15594541910331383No Hit
CCTTTCGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTCGCGGCTG40.15594541910331383No Hit
CCTTTCGGGAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA30.11695906432748539Illumina Single End Adapter 2 (100% over 34bp)
CCTTTCGGGGGTGATATCAACGCAGAGTGCAGTTCTTGATCAGGGGGCGCCT30.11695906432748539No Hit
CCTTTCGGTTATTTGGCTTGTCTTTTTCCTGCACCCTCAACTGAGTGGCTAA30.11695906432748539No Hit
CCTTTCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCCCGAAAGGAGC30.11695906432748539No Hit
CCTTTCGGGGGGGAATTCGTGGAGAAAGAAATGGCTCCCCTGGCAGCATTTT30.11695906432748539No Hit
CCTTTCGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTCGTAGCGCCGG30.11695906432748539No Hit
CCTTTCGGGCGTGCGAAAAAACATGATGATTGCAGTAAAAAAAAAAAAAAAA30.11695906432748539No Hit
CCTTTCGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAA30.11695906432748539No Hit
CCTTTCCCTCCTGTGTGTCTCGTACCTTGCTCTCTACGGTCTCTTTGGTTTC30.11695906432748539No Hit
CCTTTCGGGGGTATCAACGCAGAGTGCAGTCAAGCAGTGGTATCAACGCAGA30.11695906432748539No Hit
CCTTTCGGGCTAGCTCTTTTATGCCATACTCCAAAAAAAAAAAAAAAAAAAA30.11695906432748539No Hit
CCTTTCGGGGGGGAATTCGTGGAGAAAGAAATGGCTCCCCTGGCCGCATTTT30.11695906432748539No Hit
CCTTTCGGGGGGAATTCGTGGAGAAAGAAATGGCTCCCCTGGCCGCCTTTTT30.11695906432748539No Hit
CCTTTCGGAACGCAGAGGCGCGGGAGGACGGAGAGCAGCGGGCCCGCGCCAG30.11695906432748539No Hit
CCTTTCGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCCTTTCGGAAGCA30.11695906432748539No Hit
CCTTTCGGGGGGGAATTCGTGGAGAAAGAAATGGCTCCTCTGGGAGCATTTT30.11695906432748539No Hit
CCTTTCCCATCTCAGCAGCCTCCTTCTCAAACTTTTCGATGGTTCGCTTGTC30.11695906432748539No Hit
CCTTTCGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA30.11695906432748539Illumina Single End Adapter 2 (97% over 34bp)
CCTTTCAGGGTTGTGGTACCTGTAGGTTAGCAGACCCTCCTCCTTTCCCACC30.11695906432748539No Hit
CCTTTCGGAAGCAGTGGTATCAACACAAAGTGCAGTTCCTTTCGGGGAAAAA30.11695906432748539No Hit
CCTTTCAACCAATACTCTGTCAAAGAGCGGAAGAAACTTCCTAAAAGCTTGT30.11695906432748539No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTCCT252.5472295E-546.02
ATCCCAG252.5472295E-546.026
TCCTTTA252.5472295E-546.05
GAGGCCA252.5472295E-546.041
TCCCAGC252.5472295E-546.027
CCAACCA252.5472295E-546.036
AATCCCA252.5472295E-546.025
CTTTATC252.5472295E-546.07
TCAAGCA252.5472295E-546.045
CCTATAA252.5472295E-546.020
CCCAGCA252.5472295E-546.028
CTATAAT252.5472295E-546.021
CAGCACT252.5472295E-546.030
AAATCAA252.5472295E-546.042
CGGGGTG252.5472295E-546.06
CCAAATC252.5472295E-546.040
ACTTGCC252.5472295E-546.022
CCAGCAC252.5472295E-546.029
GCCTATA252.5472295E-546.019
CGGGGGA205.195109E-446.06
Produced by FastQC (version 0.11.5)