FastQCFastQC Report
Fri 17 Jun 2016
SRR1527616_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527616_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2888
Sequences flagged as poor quality0
Sequence length52
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTTTCGGGGTGGCTCATGCCTATAATCCCAGCACTTTGGGAGGCCAAGGAG29610.249307479224377No Hit
CCTTTCGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGG2097.236842105263158No Hit
CCTTTCGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGA1485.124653739612189No Hit
CCTTTCGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACA1444.986149584487535No Hit
CCTTTCGGAGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACA1384.778393351800554No Hit
CCTTTCCTTTATCAGCGGCAGACTTGCCACCAAGTCCAACCAAATCAAGCAA1354.674515235457063No Hit
CCTTTCGGGGACAGGATCTGGAATCCATCTGTCGTTGATCTTTCACGCTGTC1244.293628808864266No Hit
CCTTTCCCATCTTGGCTTCCTTGCTGGTCAGATTGGTCGTCTTATTACCATT883.0470914127423825No Hit
CCTTTCGCCATCAACTAACGATTCTGTCAAAAACTGACGCGTTGGATGAGGA862.9778393351800556No Hit
CCTTTCTGGGGATGGCACTGTGGAACTCTTCTTGAGATAATATTTAATGCTT862.9778393351800556No Hit
CCTTTCTTTTTGGGGTAATTATACTCATCGCGAATATCCTTAAGAGGGCGTT852.943213296398892No Hit
CCTTTCGGGGGGCGCGACTGCGTGAACGCGACATTACCCTCACTTTTGCCAC702.4238227146814406No Hit
CCTTTCGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCG662.285318559556787No Hit
CCTTTCGGGGGAATTCGTGGAGAAAGAAATGGTTCGTCTGGCAGCATTTGAT511.7659279778393353No Hit
CCTTTCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCCTTTCGGGAAG291.0041551246537395No Hit
CCTTTCTGGAGTGTCTGAAGACAGCGACAGTGTACTTACATATAATAAATAA220.7617728531855956No Hit
CCTTTCGGGCAGTATAGCGACCAGTTCACGCGGAAGGGATATAGTCGAAAAA170.5886426592797784No Hit
CCTTTCGGGGGGAATTCGTGGAGAAAGAAATGGCTCCTCTGGCAGCATTTGA140.48476454293628807No Hit
CCTTTCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA130.4501385041551247No Hit
CCTTTCGGGCGTGCGAAAAAACATGATGATTGCAGTAAAAAAAAAAAAAAAA120.41551246537396125No Hit
CCTTTCGGGGGGAATTCGTGGAGAAAGAAATGGCTCCTCTGGCAGCATTTTA110.3808864265927978No Hit
CCTTTCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCCTTTCGGGCAG90.31163434903047094No Hit
CCTTTCGGGTTAGCGCCAATTCACGGGGAAAGGGATATAGTCAAAAAAAAAA90.31163434903047094No Hit
CCTTTCGGGGACAGGATCTGGAATCCATCTGTCGTTGATCCTTCACGCTGTC90.31163434903047094No Hit
CCTTTCGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCCTTTCGGAAGCA90.31163434903047094No Hit
CCTTTCTCCACACTGTATCCAAGTTGCCTCCCAGAGGAGCCACCAGTTCTTA80.2770083102493075No Hit
CCTTTCGGGAAGCAGTGGTATCAACGCAGATCGGAAGAGCTCGTATGCCGTC80.2770083102493075Illumina Single End Adapter 2 (100% over 25bp)
CCTTTCGGGGTGTTGATATTTAAACAGATAGAACAATACAGGAAAAAAAAAA80.2770083102493075No Hit
CCTTTCGGGTCACAAAACCACACAAACCAGAACCCAAAAAAAAAAAAAAAAA70.24238227146814403No Hit
CCTTTCGGTTATTTGGCTTGTCTTTTCCTGCACCCTCAACTGAGTGGCTAAT70.24238227146814403No Hit
CCTTTCAGAGAGCCAGGCCACCACCAGGGCCCCTGGCCCGCCCACTTACATC70.24238227146814403No Hit
CCTTTCAATTTTCCTTAGCTTGTCTGAGATACTGACCTGTACATTTTGAACA70.24238227146814403No Hit
CCTTTCGGGGGAATTCGTGGAGAAAGAAATGGCTCCTCTGGCAGCATTTTAT60.20775623268698062No Hit
CCTTTCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCCTTTCGGGGAG60.20775623268698062No Hit
CCTTTCGGGAAGCAGTGATATCACGCAGGGGAAAAAAAAAAAAAAAAAAAAA60.20775623268698062No Hit
CCTTTCGGGGTGGCTCATGCCTATAATCCCAGCACTTTGGGAGGCTAAGGAG60.20775623268698062No Hit
CCTTTCGGGGTGGTATCAACGCAGTGTGCTCCTTTCGGGAAAAAAAAAAAAA50.17313019390581716No Hit
CCTTTCGGGCAGGCTCCTTTCGGGGTATCAACGCAGACTCGACTTTTTTTTT50.17313019390581716No Hit
CCTTTCGGGGGGGAATTCGTGGAGAAAGAAATGGCTCCTCTGGCAGCATTTG50.17313019390581716No Hit
CCTTTCTGGTGATTCGTCTAAGAAGTTTAAGATTGCTGAGGGTCAGTGGTAT40.13850415512465375No Hit
CCTTTCGGGGACAGGATCTGGAATCCATCTGTCGTTGATCTTTCACGCTGCC40.13850415512465375No Hit
CCTTTCGGGGGAATTCGTGGAGAAAGAAATGGCTCCTCTGGCCGCATTTGAT40.13850415512465375No Hit
CCTTTCGGGGTGCTCCTTTCGGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAA40.13850415512465375No Hit
CCTTTCGGGCACGCTAGATCTTTTATTCTATACTTAAAAAAAAAAAAAAAAA40.13850415512465375No Hit
CCTTTCGGGGGGAATTCGTGGAGAAAGAAATGGCTCCCCTGGCAGCATTTTA40.13850415512465375No Hit
CCTTTCGGGGGGACTTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGG40.13850415512465375No Hit
CCTTTCAAACTGTCTATCCAGCCTCAAACTCCACCATTTCATTTTCTGGTCT40.13850415512465375No Hit
CCTTTCTGGGGATGGCACTGTGGAACTCTTCTTGAGATAATATTTAATACTT30.10387811634349031No Hit
CCTTTCGGGGGAATTCGTGGAGAAAGAAGTGGTTCGTCTGGCAGCATTTGAT30.10387811634349031No Hit
CCTTTCAAAGTTGACATTGTAAATAAAACAACTTAAGAAAAGTATGCTGAAA30.10387811634349031No Hit
CCTTTCGGGAAGCAGTGGTATCAACGCAGAGTGCAGATCGGAAGAGCTCGTA30.10387811634349031No Hit
CCTTTCGGGTGCAGTGCTCCTTTCGAAAAAAAAAAAAAAAAAAAAAAAAAAA30.10387811634349031No Hit
CCTTTCGGGGGAATTCGTGGAGAAAGAAATGGCTCCTCTGGCAGCATTTGAT30.10387811634349031No Hit
CCTTTCGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.10387811634349031No Hit
CCTTTCGGGGGGGAATTCGTGGAGAAAGAAATGGTAATGTCGCGTTCACGCA30.10387811634349031No Hit
CCTTTCTCCACACTGTATCCAAGTTGCCCCCCAGAGGAGCCACCAGTTCTTA30.10387811634349031No Hit
CCTTTCGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTCGCGGCTG30.10387811634349031No Hit
CCTTTCGGGAAGCAGTGGTATCACAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.10387811634349031No Hit
CCTTTCGGGAGGCTAGATCTTTTATTCTATACTCAAAAAAAAAAAAAAAAAA30.10387811634349031No Hit
CCTTTCTGAGCCCCGGCGGTGCGCGCTACGGAGCTCGGCCAGCCTGCTCCGT30.10387811634349031No Hit
CCTTTCGGGGGGGATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACA30.10387811634349031No Hit
CCTTTCGGGGCAGTAGTATCAACGCAGAGTCGAAAAAAAAAAAAAAAAAAAA30.10387811634349031No Hit
CCTTTCTGGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAA30.10387811634349031No Hit
CCTTTCGGAGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGGAAAACA30.10387811634349031No Hit
CCTTTCGGGGACAGGATCTGGAATCCATCTGTCGTTGATCTTTCACACTGTC30.10387811634349031No Hit
CCTTTCGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGA30.10387811634349031No Hit
CCTTTCTCACCTGGACTGCAGGGTCTGGCTCTAAGTCACAGTGCTCCTTTCT30.10387811634349031No Hit
CCTTTCGGGAAGCAGTGGTATCAACGCGATCGGAAGAGCTCGTATGCCGTCT30.10387811634349031Illumina Single End Adapter 2 (96% over 26bp)
CCTTTCGGGGGGGAATTCGTGGAGAAAGAAATGGCTCCTCTGGCCGCATTTT30.10387811634349031No Hit
CCTTTCGGGAGACGCTTCTGGAAGGAACGCCGCGATGGCTGCGCAGGGAGAG30.10387811634349031No Hit
CCTTTCTTACCACCTCGGCCTTCACATTTGTCACAAATCACATTCTTTTGCA30.10387811634349031No Hit
CCTTTCGGGGGGAATTCCTTCGGCAACGTTAACTGGTTGATGCTGAAAAACA30.10387811634349031No Hit
CCTTTCGGGGGGGAATTCGTGGAGAAAGAAATGGCTCCTCTGGCAGCATTTT30.10387811634349031No Hit
CCTTTCAACCAATACTCTGTCAAAGAGCGGAAGAAACTTCCTAAAAGCTTGT30.10387811634349031No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGGTG550.046.0000046
TCGGGGT550.046.0000045
CTTTCCT205.3060206E-446.02
TGATGCT301.2876553E-646.038
GTTGATG301.2876553E-646.036
TGGTTGA301.2876553E-646.034
GGTTGAT301.2876553E-646.035
CGGAGGG205.3060206E-446.06
TTTCCTT205.3060206E-446.03
AACTGGT301.2876553E-646.031
CGGGGGG750.046.06
GGCAACA301.2876553E-646.022
GATGCTG301.2876553E-646.039
GAAGCAG205.3060206E-446.09
GAAAAAC301.2876553E-646.045
CAACATT301.2876553E-646.024
TCGGAGG205.3060206E-446.05
GCAACAT301.2876553E-646.023
ACATTAA301.2876553E-646.026
AGCAGTG205.3060206E-446.011