FastQCFastQC Report
Fri 17 Jun 2016
SRR1527615_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527615_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11544
Sequences flagged as poor quality0
Sequence length50
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACAAGGGCCAGAACAGCCTGATTAATGTTAAAATACTGTGTGTGTTTA4563.9501039501039505No Hit
CAACAAGGGAGATTTCTACCACTCCAAAAAAAAAAAAAAAAAAAAAAAAA3623.135828135828136No Hit
CAACAAGGGATGGCTAAGTGATGTGCCAGCATGCAGCGAGCTGGCCTGGC3392.936590436590437No Hit
CAACAAGGGATTTAAAAAAAATTTTAGGGCTGGTGAGATGGCTCAGCGAG3182.754677754677755No Hit
CAACAAGGGAGATAAGCCTTTCCTACAGGGCTGGGGATGGATAGCCTTTC3072.6593901593901594No Hit
CAACAAGGGATAAAATTTGTGACAAACTTGCTTATATAAAACATGTGTAT2772.3995148995148994No Hit
CAACAAGGGATAATTGATTTTTTTTTCCTCTTGTTTTTCAGTAAGCCCAA2712.3475398475398475No Hit
CAACAAGGGATTGGTAATGGTCTGAGCTGTGTTTTGGGCAGACCAATCTT2512.1742896742896742No Hit
CAACAAGGGATTTTTACATGGGTTAAATGTTACCACTACTTAAAATTCCT2342.027027027027027No Hit
CAACAAGGGTGGCGGCGTGCTTAACACATGCAAGTCGAACGATGAAGCCC1941.6805266805266803No Hit
CAACAAGGGATGCTACCCTGAGCTGCAGCTCCTCACTTCCACACTGAGAA1801.5592515592515594No Hit
CAACAAGAGAATCTCTACCATGAACAAAATGTGACTCATATCTAAACCAG1601.386001386001386No Hit
CAACAAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1371.1867636867636868No Hit
CAACAAGGGAGGGAAGAAAAGGAGGAGACGCCGTTGTTTGCAAATCCCAC1321.1434511434511436No Hit
CAACAAGGGATAGGTTAAGAGTTGTGTTTGTAGAGTAGAACTTGCCCAGT1291.1174636174636174No Hit
CAACAAGGGATTTAAGACATAGCCATGGAGCCCACATGTTTATGGAATAG1221.0568260568260568No Hit
CAACAAGGGAGTCACAGAAAACAACCTGTTAATTCTTTTTTCTTCTTTTT1181.022176022176022No Hit
CAACAAGGGGGGCGTCCTCACTCTGAGTACCCCTCTGTCTACGCAGCACT970.8402633402633402No Hit
CAACAAGGGTGATGATTGCCATCTCCTTTCAGGTGATGAGGTCACAGGCC930.8056133056133057No Hit
CAACAACTGAACGGACTGGAAACACTGGTCATAATCATGGTGGCGAATAA910.7882882882882882No Hit
CAACAAGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTG910.7882882882882882No Hit
CAACAATTTTAATTGCAGGGGCTTCGGCCCCTTACTTGAGGATAAATTAT910.7882882882882882No Hit
CAACAAGGGACTAGGCAGCTTTGTAACCGCCCTGGAGCCTCTGTCAAGTC850.7363132363132363No Hit
CAACAAGGGAGCCATGACCTCTCAGATTCGTCAGAATTATTCCACCGAGG850.7363132363132363No Hit
CAACAAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA800.693000693000693No Hit
CAACAAGGGCTGGAAAGCCACTATCTGGATAAGGAGGTGAAACTCATCAA760.6583506583506583No Hit
CAACAAGGGCTATGGAGCCACCGGGCACCCAGGCATCATCCCACCACATG740.641025641025641No Hit
CAACAAGGGAAGCCACTAGGCAGCTTTGTAACCGCCCTGGAGCCTCTGTC730.6323631323631324No Hit
CAACAAGGGGTATCAACGCAGAGTGCAGTGCTCAACAAGGGGTATCAACG730.6323631323631324No Hit
CAACAAGGGGCGGTCGGGCCGAGCTCGTCGCTAGGTTTGTTGAAGGACAT720.6237006237006237No Hit
CAACAAGGGGTGCAGTGCTCAACAAGGGGTTTTTTAAAATAAAATTGATG640.5544005544005544No Hit
CAACAAGGGGTTAATCTTGCAGCTGGTGGGGTTCTGGATATCCACTACTT590.511088011088011No Hit
CAACAAGGGGATTTGATCAGATTGTTAGCTTTTTCTGTTTTATTTCTTTT590.511088011088011No Hit
CAACAAGGGATTTTCTAATGTGCTTGTCTGGATGTGTCAGTATAAGTCCT570.4937629937629938No Hit
CAACAAGGGCTTTATATAAATAAAATTAAAAATAGTATGATGACACAAAA570.4937629937629938No Hit
CAACAAGGGAAAGATTTGGGACTTCTTTGTGGCATTGTGAAATAAATAAG570.4937629937629938No Hit
CAACAAGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCG560.4851004851004851No Hit
CAACAAGGGATTTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA550.4764379764379764No Hit
CAACAAGGGATAGCCTATTTAAAAATATATTTACAGATTCCCTTGTTACC540.4677754677754678No Hit
CAACAAGGGATGTTTTATGGAATTGTTCACATGATTTGAAATAATAAAAT540.4677754677754678No Hit
CAACAAGGGATTAAGTTTTTTAAAAATTAAATTGGATATTAAACTGTAAA520.45045045045045046No Hit
CAACAAGGGAGTTAAATTTTAAAAATGTTTTTTTTTTTGTAGCATGTATT520.45045045045045046No Hit
CAACAAGGGATTTCTTCAAATAAAGAATTTTGGTACCCAGAAAAAAAAAA510.4417879417879418No Hit
CAACAAGGGAGTCTTGCTGTCTCTTGGCTGAGAATAAAACCCATTTCTGG500.4331254331254331No Hit
CAACAAGGGACTTACGCATTGCCAAAGTTTTTGTTAGTCTTGCATGCTTA480.4158004158004158No Hit
CAACAAGGGGGAGACGTTCTCGCCCAGAGTCGCCGCGGTTTCCTGCTTCA470.4071379071379071No Hit
CAACAAGGGGTAAAGCATAAATAAAAAATCCTTTGTGGACTGTGAAAAAA450.38981288981288986No Hit
CAACAAGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCC430.37248787248787246No Hit
CAACAAGGGACCTCTTAAAAATAAATTTTAGCTCAGTGTGTGTGTAAAAA420.36382536382536385No Hit
CAACAAGGGAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA410.3551628551628552No Hit
CAACAAGGGGAAGCGTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGC400.3465003465003465No Hit
CAACAAGGGAGTTTTCAAAATCAGTACTTTTTAATGGAAACAACTTGACC390.33783783783783783No Hit
CAACAAGGGATACTGGGTGTTTTTGTCTTCAAATAAAAACATCACCCCTG370.3205128205128205No Hit
CAACAAGGGGGTCGTCTCTCCCCATGGCCAACGCAGCAAAAGGAAACGCG370.3205128205128205No Hit
CAACAAGGGGGGGGAGAATCCGCGTTGAAGAACAGAGCATTTAGCTCCTC360.31185031185031187No Hit
CAACAAGGGATCCCTCCCTTGCCCAAGCACCTTGTAGGTTTTGTTTTTGT360.31185031185031187No Hit
CAACAAGGGATTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA350.3031878031878032No Hit
CAACAAGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTGGTAGCGCC350.3031878031878032No Hit
CAACAAGGGATGAATGGTACCTACTAAAAAAAAAAAAAAAAAAAAAAAAA350.3031878031878032No Hit
CAACAAGGGGAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA350.3031878031878032No Hit
CAACAAGGGGACCAGAGCATGATGTTGCTGGCCAGATGACCCCTTTTGCT340.2945252945252945No Hit
CAACAAGGGATTTCTTCAAATAAAGAATTTTGGTACCCAAAAAAAAAAAA330.2858627858627859No Hit
CAACAAGGGAATTTTAAGTTAACCTGCATGCTGTAAATGTGTTTAAATAA330.2858627858627859No Hit
CAACAAGGGAGATTTCTACCACTCCAAAAAAAAAAAAAAAAAAAAAAAAG320.2772002772002772No Hit
CAACAAGGGGAGTTTGCTGATTTTAAATAAAAAGATCTCATTTTGTTTTG320.2772002772002772No Hit
CAACAAAGGATAATGTGGCATTAATAAATGTATCTGTGAAAAAAAAAAAA310.2685377685377685No Hit
CAACAAGGGACTTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA310.2685377685377685No Hit
CAACAAGGGATGCTAATAAAGCCACTGTGTCCAGAAAAAAAAAAAAAAAA300.2598752598752599No Hit
CAACAAGGGGTACAATAAATCATCTTTGCCACGAAAAAAAAAAAAAAAAA300.2598752598752599No Hit
CAACAAGGGACTCCTGATAATAAAAAATTGAAGAAATAAAAATACCCAAA300.2598752598752599No Hit
CAACAAGGGATTTGGAGAATTTCAAAAAAAAAAAAAAAAAAAAAAAAAAA290.2512127512127512No Hit
CAACAAGGGACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA290.2512127512127512No Hit
CAACAAGGGAGAGTTTCTATCTAAAAAAAAAAAAAAAAAAAAAAAAAAAA290.2512127512127512No Hit
CAACAAGGGGTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA290.2512127512127512No Hit
CAACAAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA290.2512127512127512No Hit
CAACAAGGGGACCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA280.24255024255024255No Hit
CAACAAGGGAGCTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA280.24255024255024255No Hit
CAACAAGGGCAGTGCTCAACAAGGGAAGCAACTGAAAAAAAAAAAAAAAA270.2338877338877339No Hit
CAACAAGGGATTAAAACAAGTTTAATGAGAAAAAAAAAAAAAAAAAAAAA270.2338877338877339No Hit
CAACAAGGGATTGTGAATTCCATTTAGATGTATTGTACTAAGTGAAACTT270.2338877338877339No Hit
CAACAAGGGACTTCTTATGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA270.2338877338877339No Hit
CAACAAGGGATGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA260.22522522522522523No Hit
CAACAAGGGATTGATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA260.22522522522522523No Hit
CAACAAGGGGCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT260.22522522522522523No Hit
CAACAAGGGAGCCTCAAACTTCTACTGTTTTTATCCTAATTAAACTTATG260.22522522522522523No Hit
CAACAAGGGAGTTGTTTTGAGGTCCTAACATGTACCTGAGATGTGTTCTG260.22522522522522523No Hit
CAACAAGGGATATAATAAAGATAATCCATGTTAAAAAAAAAAAAAAAAAA250.21656271656271656No Hit
CAACAAGGGATCACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA250.21656271656271656No Hit
CAACAAGGGAAGTAGTCTGAGGCCTTATAGGAGGCTCTGACAATAAATAA250.21656271656271656No Hit
CAACAAGGGATGTCACAATGTGTGTTGTGTGAATGAAAAATGAAAGTCGG240.2079002079002079No Hit
CAACAAGGGATGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA240.2079002079002079No Hit
CAACAAGGGACTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA230.19923769923769924No Hit
CAACAAGGGATTTCCTGTGTCTTTATTACTAAATAAACTACACATTTTTC230.19923769923769924No Hit
CAACAAGAGAATCTCTACCATGAACAAAATGTGACTCATATCTACACCAG220.19057519057519057No Hit
CAACAAGGGGTGCTCAACAAGGGAGTTAAAAAAAAAAAAAAAAAAAAAAA220.19057519057519057No Hit
CAACAAGGGATTTAAGTTGAATGCGAAAAAAAAAAAAAAAAAAAAAAAAA210.18191268191268192No Hit
CAACAAGGGATTGCTGTTTTAATTAAAAAAAAAAAAAAAAAAAAAAAAAA210.18191268191268192No Hit
CAACAAGGGATATCAAATGTTGATAAAAAAAAAAAAAAAAAAAAAAAAAA210.18191268191268192No Hit
CAACAAGGGAGGTTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA210.18191268191268192No Hit
CAACAAGGGAAATGCGGGTGTGTTGTACCTAACATGCACTTGTAATAAAG210.18191268191268192No Hit
CAACAAGGGAGTCTCAAGTGTTCTACACAAAAAAAAAAAAAAAAAAAAAA210.18191268191268192No Hit
CAACAAGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTT210.18191268191268192No Hit
CAACAAGGGGGGTCATTGAGAGAAATAAAGATCGCTTGGAAAAGAAAAAA210.18191268191268192No Hit
CAACAAGGGAAGTAAAAATAAAGCTTTTTGAGACAGCAAAAAAAAAAAAA210.18191268191268192No Hit
CAACAAGGGACTGAATAAAAACTTGAGACTGTCAAAAAAAAAAAAAAAAA210.18191268191268192No Hit
CAACAAGGGAGAACTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA200.17325017325017325No Hit
CAACAAGGGAACCAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA200.17325017325017325No Hit
CAACAAGGGATTACTTAAAGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAA190.16458766458766458No Hit
CAACAAGGGACGTGCTTCTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA190.16458766458766458No Hit
CAACAAGGGATTGGGGATCGATCACACTGGACCTGTTGTGCAGATGGTTT190.16458766458766458No Hit
CAACAAGGGGTATCAACGCAGAGTGCAAGCTCAACAAGGGTAAAAAAAAA190.16458766458766458No Hit
CAACAAGGGATTTATTGAATACTTTGATTTTATGAAATAAAAACTAAATA190.16458766458766458No Hit
CAACAAGGGAGTGTGTTTTGGTCAAAAAAAAAAAAAAAAAAAAAAAAAAA180.15592515592515593No Hit
CAACAAGGGACTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA180.15592515592515593No Hit
CAACAAGGGATTCTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA170.14726264726264726No Hit
CAACAAGGGATTCCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA170.14726264726264726No Hit
CAACAAGGGATAATTGATTTTTTTTCCTCTTGTTTTTCAGTAAGCCCAAT170.14726264726264726No Hit
CAACAAGGGATTATTGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA170.14726264726264726No Hit
CAACAAGGGATTCCTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA170.14726264726264726No Hit
CAACAAGGGAGTTTCATGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA170.14726264726264726No Hit
CAACAAGGGATTCTTTGATCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA170.14726264726264726No Hit
CAACAAGGGAAGTTTTATTATTGCAATAAAAGTGCTTTATGCTGGCTTTT170.14726264726264726No Hit
CAACAAGGGATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA170.14726264726264726No Hit
CAACAAGGGATTGTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA170.14726264726264726No Hit
CAACAAGGGAATTACAGTTTGTTAATTTGAAAAAAAAAAAAAAAAAAAAA160.1386001386001386No Hit
CAACAAGGGATCCCCGTCTGTGTCTGTGAAAAAAAAAAAAAAAAAAAAAA160.1386001386001386No Hit
CAACAAGACCGGGGAGGAGGCTGAAAACATCGTGGTCACCACCGGGGTGG160.1386001386001386No Hit
CAACAAGGGACGCAGAGTGCAGTGCTCAACAAGGGGCTCAACAAGGGGGA150.12993762993762994No Hit
CAACAAGGGATTGCCCTTGCTTTTGAAGGAAGAAGAATGAAACTGTGTAC150.12993762993762994No Hit
CAACAAGGGAAGTTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA150.12993762993762994No Hit
CAACAAGGGTACCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA150.12993762993762994No Hit
CAACAAGGGATTTTAAAGATAAGGAGAGAGAAACTAGCATACAGAATTTT150.12993762993762994No Hit
CAACAAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA150.12993762993762994No Hit
CAACAAGGGGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA150.12993762993762994No Hit
CAACAAGGGACTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA140.12127512127512127No Hit
CAACAAGGGATCTTGTTTTTGTTTTGGCTTTTTTTTTTTTTTGTTTCAGT140.12127512127512127No Hit
CAACAAGGGGTTGTGTGTGTGACTGAGAAAAAAAAAAAAAAAAAAAAAAA140.12127512127512127No Hit
CAACAAGGGATGGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA140.12127512127512127No Hit
CAACAAGGGAACCATGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA140.12127512127512127No Hit
CAACAAGGGCTGCTTCTCTTCTGACTGGGAAATAAACACCCCTGAAGGAA130.11261261261261261No Hit
CAACAAGGGATTCTCTCTATACTTTCTTCCTTTTCTCTTGGGAAAATATA130.11261261261261261No Hit
CAACAAGGGGAATCGGGCGCGCCATGAGTTAGCCAACAACGGTAAGTTAC130.11261261261261261No Hit
CAACAAGGGATCTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA120.10395010395010396No Hit
CAACAAGGGATCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA120.10395010395010396No Hit
CAACAAGGGATCTTGTTTTTGTTTTGGCTTTTTTTTTTTTTGTTTCAGTT120.10395010395010396No Hit
CAACAAGGGATTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA120.10395010395010396No Hit
CAACAAGGGATGATATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA120.10395010395010396No Hit
CAACAAGGGATTGAAACAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA120.10395010395010396No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGCTCC207.535489E-444.00000426
AGGGGTG207.535489E-444.0000046
CTACAGG551.8189894E-1244.00000423
CTTTCCT551.8189894E-1244.00000418
TGGTCTG407.1504473E-944.00000418
GATGGAT551.8189894E-1244.00000436
TTTTGGT207.535489E-444.00000428
TTTTGGG407.1504473E-944.00000432
AGGGGGT207.535489E-444.0000046
GTTTGTA207.535489E-444.00000426
AGGGGCG207.535489E-444.0000046
AGGGGAG207.535489E-444.0000046
CAAGAGA207.535489E-444.0000044
ACAAAAT207.535489E-444.00000424
GTGTTTG207.535489E-444.00000424
AAACCAG207.535489E-444.00000444
AACAAGA207.535489E-444.0000042
ATCTCTA207.535489E-444.00000411
CCAATCT407.1504473E-944.00000443
CATGAAC207.535489E-444.00000419
Produced by FastQC (version 0.11.5)