FastQCFastQC Report
Fri 17 Jun 2016
SRR1527614_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527614_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9280
Sequences flagged as poor quality0
Sequence length52
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACAAGGGCCAGAACAGCCTGATTAATGTTAAAATACTGTGTGTGTTTATG3203.4482758620689653No Hit
CAACAAGGGATGGCTAAGTGATGTGCCAGCATGCAGCGAGCTGGCCTGGCTG2692.898706896551724No Hit
CAACAAGGGATTTAAAAAAAATTTTAGGGCTGGTGAGATGGCTCAGCGAGTA2552.747844827586207No Hit
CAACAAGGGAGATTTCTACCACTCCAAAAAAAAAAAAAAAAAAAAAAAAAAA2462.6508620689655173No Hit
CAACAAGGGATAATTGATTTTTTTTTCCTCTTGTTTTTCAGTAAGCCCAATA2412.5969827586206895No Hit
CAACAAGGGAGATAAGCCTTTCCTACAGGGCTGGGGATGGATAGCCTTTCTT2362.543103448275862No Hit
CAACAAGGGATAAAATTTGTGACAAACTTGCTTATATAAAACATGTGTATTG2062.2198275862068964No Hit
CAACAAGGGATTGGTAATGGTCTGAGCTGTGTTTTGGGCAGACCAATCTTCT1751.8857758620689655No Hit
CAACAAGGGATTTTTACATGGGTTAAATGTTACCACTACTTAAAATTCCTGT1641.767241379310345No Hit
CAACAAGGGTGGCGGCGTGCTTAACACATGCAAGTCGAACGATGAAGCCCAG1621.7456896551724137No Hit
CAACAAGGGATGCTACCCTGAGCTGCAGCTCCTCACTTCCACACTGAGAATA1551.670258620689655No Hit
CAACAAGAGAATCTCTACCATGAACAAAATGTGACTCATATCTAAACCAGTC1411.519396551724138No Hit
CAACAAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1261.357758620689655No Hit
CAACAAGGGAGGGAAGAAAAGGAGGAGACGCCGTTGTTTGCAAATCCCACCT1241.3362068965517242No Hit
CAACAAGGGATTTAAGACATAGCCATGGAGCCCACATGTTTATGGAATAGTC1141.228448275862069No Hit
CAACAAGGGATAGGTTAAGAGTTGTGTTTGTAGAGTAGAACTTGCCCAGTGT1051.1314655172413792No Hit
CAACAAGGGAGTCACAGAAAACAACCTGTTAATTCTTTTTTCTTCTTTTTTT1021.0991379310344827No Hit
CAACAATTTTAATTGCAGGGGCTTCGGCCCCTTACTTGAGGATAAATTATGT981.0560344827586208No Hit
CAACAAGGGCTTTATATAAATAAAATTAAAAATAGTATGATGACACAAAAAC900.9698275862068966No Hit
CAACAACTGAACGGACTGGAAACACTGGTCATAATCATGGTGGCGAATAAGT870.9375No Hit
CAACAAGGGGGGCGTCCTCACTCTGAGTACCCCTCTGTCTACGCAGCACTGA860.9267241379310345No Hit
CAACAAGGGAGCCATGACCTCTCAGATTCGTCAGAATTATTCCACCGAGGTG710.7650862068965517No Hit
CAACAAGGGGATTTGATCAGATTGTTAGCTTTTTCTGTTTTATTTCTTTTGA660.7112068965517242No Hit
CAACAAGGGAAGCCACTAGGCAGCTTTGTAACCGCCCTGGAGCCTCTGTCAA650.7004310344827587No Hit
CAACAAGGGTGATGATTGCCATCTCCTTTCAGGTGATGAGGTCACAGGCCTG630.6788793103448275No Hit
CAACAAGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG620.6681034482758621No Hit
CAACAAGGGGCGGTCGGGCCGAGCTCGTCGCTAGGTTTGTTGAAGGACATCG590.6357758620689655No Hit
CAACAAGGGGTTAATCTTGCAGCTGGTGGGGTTCTGGATATCCACTACTTAG590.6357758620689655No Hit
CAACAAGGGAGTTTTCAAAATCAGTACTTTTTAATGGAAACAACTTGACCAA580.625No Hit
CAACAAGGGCTGGAAAGCCACTATCTGGATAAGGAGGTGAAACTCATCAAGA570.6142241379310345No Hit
CAACAAGGGGTGCAGTGCTCAACAAGGGGTTTTTTAAAATAAAATTGATGTT570.6142241379310345No Hit
CAACAAGGGCTATGGAGCCACCGGGCACCCAGGCATCATCCCACCACATGCC550.5926724137931034No Hit
CAACAAGGGACTAGGCAGCTTTGTAACCGCCCTGGAGCCTCTGTCAAGTCTT540.581896551724138No Hit
CAACAAGGGAGTTAAATTTTAAAAATGTTTTTTTTTTTGTAGCATGTATTGT540.581896551724138No Hit
CAACAAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA510.5495689655172413No Hit
CAACAAGGGATTTCTTCAAATAAAGAATTTTGGTACCCAAAAAAAAAAAAAA500.5387931034482759No Hit
CAACAAGGGATAGCCTATTTAAAAATATATTTACAGATTCCCTTGTTACCTT490.5280172413793104No Hit
CAACAAGGGAGTCTTGCTGTCTCTTGGCTGAGAATAAAACCCATTTCTGGAT470.5064655172413792No Hit
CAACAAGGGACTTACGCATTGCCAAAGTTTTTGTTAGTCTTGCATGCTTAAT430.46336206896551724No Hit
CAACAAGGGATTTCTTCAAATAAAGAATTTTGGTACCCAGAAAAAAAAAAAA430.46336206896551724No Hit
CAACAAGGGATACTGGGTGTTTTTGTCTTCAAATAAAAACATCACCCCTGCC430.46336206896551724No Hit
CAACAAGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCG430.46336206896551724No Hit
CAACAAGGGGGAGACGTTCTCGCCCAGAGTCGCCGCGGTTTCCTGCTTCAAC420.45258620689655177No Hit
CAACAAGGGATTAAGTTTTTTAAAAATTAAATTGGATATTAAACTGTAAAAA420.45258620689655177No Hit
CAACAAGGGGTAAAGCATAAATAAAAAATCCTTTGTGGACTGTGAAAAAAAA410.44181034482758624No Hit
CAACAAGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGG410.44181034482758624No Hit
CAACAAGGGATTTTCTAATGTGCTTGTCTGGATGTGTCAGTATAAGTCCTAG400.43103448275862066No Hit
CAACAAGGGATCCCTCCCTTGCCCAAGCACCTTGTAGGTTTTGTTTTTGTTT390.42025862068965514No Hit
CAACAAGGGAGTTGTTTTGAGGTCCTAACATGTACCTGAGATGTGTTCTGCA380.40948275862068967No Hit
CAACAAGGGGAAGCGTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCCC380.40948275862068967No Hit
CAACAAGGGATTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA370.39870689655172414No Hit
CAACAAGGGAGATTTCTACCACTCCAAAAAAAAAAAAAAAAAAAAAAAAAAG370.39870689655172414No Hit
CAACAAGGGATTTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA360.3879310344827586No Hit
CAACAAGGGAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA350.3771551724137931No Hit
CAACAAGGGATTTCCTGTGTCTTTATTACTAAATAAACTACACATTTTTCTC350.3771551724137931No Hit
CAACAAGGGAAAGATTTGGGACTTCTTTGTGGCATTGTGAAATAAATAAGCC350.3771551724137931No Hit
CAACAAGGGACTTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA340.36637931034482757No Hit
CAACAAGGGGGGGGAGAATCCGCGTTGAAGAACAGAGCATTTAGCTCCTCTG320.3448275862068966No Hit
CAACAAGGGATTTGGAGAATTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA320.3448275862068966No Hit
CAACAAGGGGCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT310.33405172413793105No Hit
CAACAAGGGATGTCACAATGTGTGTTGTGTGAATGAAAAATGAAAGTCGGAA300.3232758620689655No Hit
CAACAAGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTGGTAGCGCCGG290.3125No Hit
CAACAAGGGGGTCGTCTCTCCCCATGGCCAACGCAGCAAAAGGAAACGCGCC290.3125No Hit
CAACAAGGGACCTCTTAAAAATAAATTTTAGCTCAGTGTGTGTGTAAAAAAA280.3017241379310345No Hit
CAACAAGGGGACCAGAGCATGATGTTGCTGGCCAGATGACCCCTTTTGCTGT270.290948275862069No Hit
CAACAAGGGAGCCTCAAACTTCTACTGTTTTTATCCTAATTAAACTTATGAA270.290948275862069No Hit
CAACAAGGGAAGTAAAAATAAAGCTTTTTGAGACAGCAAAAAAAAAAAAAAA270.290948275862069No Hit
CAACAAGGGATGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA270.290948275862069No Hit
CAACAAGGGGAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA260.2801724137931034No Hit
CAACAAGGGAAATGCGGGTGTGTTGTACCTAACATGCACTTGTAATAAAGCC260.2801724137931034No Hit
CAACAAGGGATGTTTTATGGAATTGTTCACATGATTTGAAATAATAAAATGT260.2801724137931034No Hit
CAACAAGGGGACCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA250.26939655172413796No Hit
CAACAAGGGATATAATAAAGATAATCCATGTTAAAAAAAAAAAAAAAAAAAA240.25862068965517243No Hit
CAACAAGGGATTGTGAATTCCATTTAGATGTATTGTACTAAGTGAAACTTGT240.25862068965517243No Hit
CAACAAGGGACTTCTTATGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA240.25862068965517243No Hit
CAACAAGGGGTACAATAAATCATCTTTGCCACGAAAAAAAAAAAAAAAAAAA240.25862068965517243No Hit
CAACAAGGGACTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA240.25862068965517243No Hit
CAACAAGGGACTGAATAAAAACTTGAGACTGTCAAAAAAAAAAAAAAAAAAA240.25862068965517243No Hit
CAACAAGGGAATTTTAAGTTAACCTGCATGCTGTAAATGTGTTTAAATAAAG230.2478448275862069No Hit
CAACAAGGGATGCTAATAAAGCCACTGTGTCCAGAAAAAAAAAAAAAAAAAA230.2478448275862069No Hit
CAACAAGGGACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA230.2478448275862069No Hit
CAACAAGGGATTAAAACAAGTTTAATGAGAAAAAAAAAAAAAAAAAAAAAAA220.23706896551724138No Hit
CAACAAGGGGTATCAACGCAGAGTGCAGTGCTCAACAAGGGGTATCAACGCA210.22629310344827588No Hit
CAACAAGGGATCTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA210.22629310344827588No Hit
CAACAAGGGATGAATGGTACCTACTAAAAAAAAAAAAAAAAAAAAAAAAAAA210.22629310344827588No Hit
CAACAAGGGATTGATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA210.22629310344827588No Hit
CAACAAGGGGTATCAACGCAGAGTGCAAGCTCAACAAGGGTAAAAAAAAAAA210.22629310344827588No Hit
CAACAAGGGACTCCTGATAATAAAAAATTGAAGAAATAAAAATACCCAAAAC210.22629310344827588No Hit
CAACAAGGGAGCTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA200.21551724137931033No Hit
CAACAAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA200.21551724137931033Illumina Single End Adapter 2 (100% over 34bp)
CAACAAGGGAGATTTCTACCACTCCAAAAAAAAAAAAAAAAAAAAAAAAAGG200.21551724137931033No Hit
CAACAAGGGAGAACTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA190.20474137931034483No Hit
CAACAAGGGAACCAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA190.20474137931034483No Hit
CAACAAGGGATTCCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA180.1939655172413793No Hit
CAACAAGGGATTTAAGTTGAATGCGAAAAAAAAAAAAAAAAAAAAAAAAAAA180.1939655172413793No Hit
CAACAAGGGCTGCTTCTCTTCTGACTGGGAAATAAACACCCCTGAAGGAAAA180.1939655172413793No Hit
CAACAAAGGATAATGTGGCATTAATAAATGTATCTGTGAAAAAAAAAAAAAA180.1939655172413793No Hit
CAACAAGGGGAGTTTGCTGATTTTAAATAAAAAGATCTCATTTTGTTTTGCA180.1939655172413793No Hit
CAACAAGGGGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA170.18318965517241378No Hit
CAACAAGGGGTGCTCAACAAGGGAGTTAAAAAAAAAAAAAAAAAAAAAAAAA170.18318965517241378No Hit
CAACAAGGGGGGTCATTGAGAGAAATAAAGATCGCTTGGAAAAGAAAAAAAA170.18318965517241378No Hit
CAACAAGGGGAAAGAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA170.18318965517241378No Hit
CAACAAGGGAGTTTCATGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA170.18318965517241378No Hit
CAACAAGGGATTGAAACAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA170.18318965517241378No Hit
CAACAAGGGAAGTTTTATTATTGCAATAAAAGTGCTTTATGCTGGCTTTTCT160.1724137931034483No Hit
CAACAAGGGAGGTTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA160.1724137931034483No Hit
CAACAAGAGAGTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA160.1724137931034483No Hit
CAACAAGGGATTGCCCTTGCTTTTGAAGGAAGAAGAATGAAACTGTGTACTT160.1724137931034483No Hit
CAACAAGGGACGCAGAGTGCAGTGCTCAACAAGGGGCTCAACAAGGGGGAGG160.1724137931034483No Hit
CAACAAGGGATTTATTGAATACTTTGATTTTATGAAATAAAAACTAAATATC160.1724137931034483No Hit
CAACAAGGGATATCAAATGTTGATAAAAAAAAAAAAAAAAAAAAAAAAAAAA150.16163793103448276No Hit
CAACAAGGGAAGTAGTCTGAGGCCTTATAGGAGGCTCTGACAATAAATAAAT150.16163793103448276No Hit
CAACAAGGGATTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA150.16163793103448276No Hit
CAACAAGGGATAATTGATTTTTTTTCCTCTTGTTTTTCAGTAAGCCCAATAA140.15086206896551724No Hit
CAACAAGGGAACCATGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA140.15086206896551724No Hit
CAACAAGGGGTTGTGTGTGTGACTGAGAAAAAAAAAAAAAAAAAAAAAAAAA140.15086206896551724No Hit
CAACAAGGGATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA140.15086206896551724No Hit
CAACAAGACCGGGGAGGAGGCTGAAAACATCGTGGTCACCACCGGGGTGGTG140.15086206896551724No Hit
CAACAAGGGAAGCGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA140.15086206896551724No Hit
CAACAAGGGAAGTTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA140.15086206896551724No Hit
CAACAAGGGATTGCTGTTTTAATTAAAAAAAAAAAAAAAAAAAAAAAAAAAA140.15086206896551724No Hit
CAACAAGGGGAATCGGGCGCGCCATGAGTTAGCCAACAACGGTAAGTTACCA140.15086206896551724No Hit
CAACAAGGGATGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA140.15086206896551724No Hit
CAACAAGGGAGTGTGTTTTGGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA130.1400862068965517No Hit
CAACAAGGGATGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA130.1400862068965517No Hit
CAACAAGGGACCATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA130.1400862068965517No Hit
CAACAAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA130.1400862068965517No Hit
CAACAAGGGATGATATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA130.1400862068965517No Hit
CAACAAGGGATTACTTAAAGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA130.1400862068965517No Hit
CAACAAGGGAGTTAAATTTTAAAAATGTTTTTTTTTTGTAGCATGTATTGTA130.1400862068965517No Hit
CAACAAGGGATTGGGGATCGATCACACTGGACCTGTTGTGCAGATGGTTTTC130.1400862068965517No Hit
CAACAAGGGATTCTTTTTTGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA130.1400862068965517No Hit
CAACAAGGGCAGTGCTCAACAAGGGAAGCAACTGAAAAAAAAAAAAAAAAAA120.12931034482758622No Hit
CAACAAGGGATTCCTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA120.12931034482758622No Hit
CAACAAGGGATTCTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA120.12931034482758622No Hit
CAACAAGGGAATTACAGTTTGTTAATTTGAAAAAAAAAAAAAAAAAAAAAAA120.12931034482758622No Hit
CAACAAGGGATTCTTTGATCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA120.12931034482758622No Hit
CAACAAGGGTACCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA120.12931034482758622No Hit
CAACAAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA120.12931034482758622No Hit
CAACAAGGGAGAGTTTCTATCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA110.11853448275862069No Hit
CAACAAGGGATCTTGTTTTTGTTTTGGCTTTTTTTTTTTTTTTTTTCAGTTT110.11853448275862069No Hit
CAACAAGGGATGGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA110.11853448275862069No Hit
CAACAAGGGATTATTGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA100.10775862068965517No Hit
CAACAAGGGATCCCCGTCTGTGTCTGTGAAAAAAAAAAAAAAAAAAAAAAAA100.10775862068965517No Hit
CAACAAGGGATCACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA100.10775862068965517No Hit
CAACAAGGGATTGTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA100.10775862068965517No Hit
CAACAAGGGATAATTGATTTTTTTTTTCCTCTTGTTTTTCAGTAAGCCCAAT100.10775862068965517No Hit
CAACAAGGGATCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA100.10775862068965517No Hit
CAACAAGGGCATGCCACTGCACTCTCCATCTCAAAAAAAAAAAAAAAAAAAA100.10775862068965517No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTCTG358.806455E-846.00000418
AATCTTC358.806455E-846.00000445
ATCTTCT358.806455E-846.00000446
TGGTAAT358.806455E-846.00000412
GGTCTGA358.806455E-846.00000419
ATTGGTA358.806455E-846.00000410
ATGGTCT358.806455E-846.00000417
ACCAATC358.806455E-846.00000442
GGATTGG358.806455E-846.0000048
GAGCTGT358.806455E-846.00000424
TAATGGT358.806455E-846.00000415
GTCTGAG358.806455E-846.00000420
AGGGATG750.046.0000046
GATTGGT358.806455E-846.0000049
AGGGACT358.806455E-846.0000046
TTGGGCA358.806455E-846.00000434
GCTGTGT358.806455E-846.00000426
CTGTGTT358.806455E-846.00000427
AATGGTC358.806455E-846.00000416
TCTGAGC358.806455E-846.00000421
Produced by FastQC (version 0.11.5)