FastQCFastQC Report
Fri 17 Jun 2016
SRR1527609_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527609_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences700983
Sequences flagged as poor quality0
Sequence length50
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGTTGGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT49120.7007302602202906No Hit
ACGTTGGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC39940.5697713068647884No Hit
ACGTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC37400.5335364766335275No Hit
ACGTTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTC36900.5264036360368225No Hit
ACGTTGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCC30020.428255749426163No Hit
ACGTTGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAA24550.35022247329821127No Hit
ACGTTGGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTG23610.336812732976406No Hit
ACGTTGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC19580.27932203776696435No Hit
ACGTTGGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCA18820.2684801200599729No Hit
ACGTTGGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAG18090.25806617278878374No Hit
ACGTTGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGA17990.25663960466944274No Hit
ACGTTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG17860.25478506611429946No Hit
ACGTTGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTT12810.18274337608757987No Hit
ACGTTGGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCG12070.17218677200445662No Hit
ACGTTGGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCG12050.1719014583805884No Hit
ACGTTGGGGGGGGGCTGGTGAGATGGCTCAGCGGTTAAGAACACTGACTG11650.16619518590322446No Hit
ACGTTGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGA10880.1552106113842989No Hit
ACGTTGGGGGTCCCTGTCTTACACCACAATATTTAAACTTGTCTTCCTTT10710.15278544558141924No Hit
ACGTTGGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATC10470.1493616820950009No Hit
ACGTTGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAG10330.14736448672792352No Hit
ACGTTGGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTA10210.14565260498471433No Hit
ACGTTGGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCA10010.14279946874603236No Hit
ACGTTGGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGC8550.12197157420365402No Hit
ACGTTGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGC8280.11811984028143335No Hit
ACGTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGT8150.11626530172629007No Hit
ACGTTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTG7810.11141497012053074No Hit
ACGTTGGGGGAGTTGCTTCAGTGTCCTGGTGCGGAGCGTCACGTCTCGTG7700.10984574518925565No Hit
ACGTTGGGGGGGGCTGGAGAGATGGCTCAACGGTTAAGAGCACTGACTGC7620.10870449069378288No Hit
ACGTTGGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGT7580.10813386344604649No Hit
ACGTTGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCT7500.10699260895057369No Hit
ACGTTGGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCG7470.10656463851477141No Hit
ACGTTGGGGGATAGTAGAGGCCACAGGAAGACCGTCGTGGAGAAGCCCCG7440.10613666807896911No Hit
ACGTTGGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCTT7420.10585135445510092No Hit
ACGTTGGGGGGGCTGCCCGTGACCTGCCTGGGCGCAGGGAACGGAAAGTC7260.10356884546415535No Hit
ACGTTGGGAAAAGATGGGGTTTAATGAGGTGACCTGTAGCTTTGGCACGT7250.10342618865222122No Hit
ACGTTGGGGGGGGCTGGAGAGATGGCTCAGCGTTTAAGAGCACTGACTGC7060.10071570922547336No Hit
ACGTTGGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTT7060.10071570922547336No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAATA302.526298E-644.019
TGATAGA302.526298E-644.026
ACACGTA254.4410575E-544.019
CATATGC600.044.033
GTATTAG207.8543776E-444.010
CACGGTT207.8543776E-444.040
GTACACC254.4410575E-544.041
CGAAATT207.8543776E-444.041
CGAAAGT254.4410575E-544.016
CGAAACT750.044.042
CCCTATT207.8543776E-444.010
GCGAAAC207.8543776E-444.043
TATGGTT254.4410575E-544.014
TATGGTA207.8543776E-444.011
GTCCTAT254.4410575E-544.017
GACGTAA207.8543776E-444.09
CGCATAT207.8543776E-444.041
GCTATAT254.4410575E-544.020
TTCACGA207.8543776E-444.037
TCTTAAC207.8543776E-444.037
Produced by FastQC (version 0.11.5)