##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527609_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	700983
Sequences flagged as poor quality	0
Sequence length	50
%GC	58
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.16260594051496	31.0	30.0	33.0	26.0	33.0
2	31.42102732876546	31.0	31.0	33.0	30.0	34.0
3	32.02079080377128	31.0	31.0	34.0	31.0	34.0
4	34.99930240818964	35.0	35.0	37.0	33.0	37.0
5	35.47435529820267	35.0	35.0	37.0	35.0	37.0
6	35.896566678507185	37.0	35.0	37.0	35.0	37.0
7	36.52123375317233	37.0	37.0	37.0	35.0	37.0
8	36.642295176915844	37.0	37.0	37.0	35.0	37.0
9	38.496566963820804	39.0	39.0	39.0	37.0	39.0
10	37.12702305191424	39.0	37.0	39.0	34.0	39.0
11	36.05237930163784	37.0	35.0	39.0	32.0	39.0
12	35.94557642624714	37.0	35.0	39.0	32.0	39.0
13	36.149478660680785	38.0	35.0	39.0	32.0	39.0
14	37.017280019629574	39.0	35.0	40.0	33.0	40.0
15	37.176299567892514	39.0	35.0	40.0	33.0	40.0
16	37.331286208082076	39.0	35.0	40.0	33.0	40.0
17	37.25590920179234	39.0	35.0	40.0	33.0	40.0
18	37.079603927627346	39.0	35.0	40.0	33.0	40.0
19	36.998756032599935	39.0	35.0	40.0	33.0	40.0
20	36.773408199628236	38.0	35.0	40.0	33.0	40.0
21	36.70532951583704	38.0	35.0	40.0	33.0	40.0
22	36.517517543221444	38.0	35.0	40.0	32.0	40.0
23	36.257213085053415	38.0	35.0	40.0	32.0	40.0
24	36.06880623353205	38.0	35.0	40.0	31.0	40.0
25	35.80893973177666	37.0	34.0	39.0	31.0	40.0
26	35.17058473600644	36.0	34.0	39.0	30.0	40.0
27	35.01367222885576	36.0	34.0	38.0	30.0	40.0
28	34.849130435402856	36.0	33.0	38.0	30.0	40.0
29	34.53016406959941	36.0	33.0	38.0	29.0	40.0
30	34.1909176114114	35.0	33.0	38.0	28.0	39.0
31	34.004119928728656	35.0	33.0	38.0	27.0	39.0
32	33.944242585055555	35.0	33.0	38.0	27.0	39.0
33	33.3030900892033	35.0	32.0	38.0	25.0	39.0
34	32.8134933372136	34.0	31.0	37.0	24.0	39.0
35	32.59447518698742	34.0	31.0	37.0	23.0	38.0
36	32.211654205594144	34.0	31.0	37.0	23.0	38.0
37	31.88542518149513	34.0	30.0	36.0	23.0	38.0
38	31.35560919451684	33.0	30.0	36.0	21.0	38.0
39	30.538157130772074	33.0	29.0	35.0	20.0	38.0
40	30.252864905425668	33.0	28.0	35.0	19.0	38.0
41	29.9129351211085	32.0	28.0	35.0	18.0	38.0
42	29.52902994794453	32.0	27.0	34.0	18.0	37.0
43	29.09903235884465	31.0	26.0	34.0	18.0	37.0
44	28.6620003623483	31.0	26.0	34.0	18.0	36.0
45	28.300470910136195	30.0	25.0	34.0	18.0	36.0
46	28.06612713860393	30.0	24.0	33.0	18.0	35.0
47	27.53521840044623	30.0	23.0	33.0	17.0	35.0
48	27.020494077602454	29.0	23.0	33.0	15.0	35.0
49	26.298713378213165	29.0	23.0	32.0	13.0	34.0
50	29.583240963047604	32.0	27.0	34.0	20.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	3.0
13	8.0
14	28.0
15	54.0
16	140.0
17	227.0
18	409.0
19	762.0
20	1303.0
21	1866.0
22	2685.0
23	4000.0
24	5245.0
25	7221.0
26	10035.0
27	13568.0
28	18000.0
29	22689.0
30	29152.0
31	43332.0
32	68547.0
33	81600.0
34	88311.0
35	144566.0
36	136300.0
37	20536.0
38	395.0
39	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	100.0	0.0	0.0
2	0.0	0.0	0.0	100.0
3	100.0	0.0	0.0	0.0
4	0.0	0.0	100.0	0.0
5	0.0	0.0	100.0	0.0
6	100.0	0.0	0.0	0.0
7	99.23336229266616	0.5774747747092298	0.10385415908802353	0.08530877353659076
8	99.29499003542168	0.5178442273207766	0.09329755500490026	0.09386818225263666
9	95.77322131920461	2.38650580684553	0.9980270562909513	0.842245817658916
10	60.62786116068435	27.704808818473488	4.970305984595917	6.697024036246242
11	35.81927093809693	28.418948819015583	17.237650556432897	18.524129686454593
12	29.60271504444473	21.934055462115342	24.807734281715817	23.655495211724105
13	23.75621092094958	19.72458675888003	28.265878059810294	28.253324260360095
14	24.269632787100402	20.306769208383084	28.6587549198768	26.76484308463971
15	25.52586867299207	20.582810139475566	26.194358493715253	27.696962693817113
16	29.502997932902797	20.72118724705164	23.13337128004531	26.642443540000254
17	31.6914960847838	21.48882355206902	20.675394410420793	26.14428595272639
18	30.7414017173027	21.05628809828484	21.590537858978035	26.611772325434423
19	30.36906743815471	21.68540463891421	20.096207753968358	27.849320168962727
20	29.835102991085378	23.341364911845222	20.4030625564386	26.420469540630798
21	31.815892824790332	21.610937783084612	21.579125314023308	24.99404407810175
22	31.844566843989085	21.31392630063782	21.879988530392318	24.961518324980776
23	31.872242265504298	21.378549836443966	20.02958702279513	26.719620875256606
24	28.800270477315426	22.24048229414979	22.144759573342007	26.81448765519278
25	29.09770993019802	22.011803424619426	21.735334523091147	27.15515212209141
26	28.441631252113105	21.274410363732073	21.656302649279656	28.62765573487517
27	28.834936082615414	21.067415329615695	21.06013983220706	29.03750875556183
28	31.420590798920944	22.23263616949341	19.270224812869927	27.07654821871572
29	31.251114506343235	22.88243794785323	19.230566219152248	26.63588132665129
30	29.339085255990515	22.385136301450963	19.320582667482665	28.955195775075858
31	32.08779670833672	22.85248001734707	18.76693158036643	26.29279169394978
32	34.3285072533856	21.733479984536004	19.1968992115358	24.741113550542597
33	32.70792586981425	22.961897792100523	20.081514102339142	24.24866223574609
34	30.80017632381955	22.9576180877425	23.642085471402304	22.60012011703565
35	29.989885632033875	24.53625836860523	22.156885402356405	23.31697059700449
36	31.386781134492566	24.5490974816793	19.087338779970413	24.976782603857725
37	31.36252947646377	24.71800314700927	18.731552691006772	25.18791468552019
38	32.79366261378664	24.247948951686418	19.675084845138898	23.283303589388048
39	32.20834171442103	23.037220588801727	19.295475068582263	25.458962628194975
40	31.021009068693534	21.640325086343033	21.132894806293447	26.205771038669983
41	28.759898599538076	22.14105049623172	21.11520536161362	27.98384554261658
42	27.885127028758188	19.639420642155372	22.965464212398874	29.509988116687563
43	28.635787173155414	19.59063201247391	23.668191667986243	28.105389146384436
44	27.0982320541297	19.14639870011113	24.5616512811295	29.193717964629666
45	29.036510157878293	19.10602682233378	24.52755630307725	27.329906716710678
46	29.320539870439084	20.929323535663492	22.434638215192095	27.315498378705332
47	29.334520238008626	20.455845576854216	22.828798986566007	27.380835198571152
48	29.795587054179627	21.044019612458506	22.858614260260236	26.301779073101628
49	28.943783230121127	20.16468302369672	24.36735270327526	26.524181042906893
50	27.088674047730116	20.60691914069243	24.053079746584437	28.251327064993013
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.5
6	1.0
7	4.0
8	7.0
9	50.0
10	93.0
11	341.5
12	590.0
13	463.5
14	337.0
15	428.5
16	520.0
17	626.5
18	733.0
19	1006.0
20	1279.0
21	1322.5
22	1366.0
23	1870.5
24	2375.0
25	3025.5
26	3676.0
27	4238.5
28	4801.0
29	5366.0
30	5931.0
31	6145.5
32	6360.0
33	6551.0
34	6742.0
35	7262.5
36	7783.0
37	8157.5
38	8532.0
39	8481.5
40	8431.0
41	9511.5
42	10592.0
43	11315.0
44	12038.0
45	13598.0
46	15158.0
47	17289.5
48	19421.0
49	19979.5
50	20538.0
51	24827.5
52	29117.0
53	31155.0
54	33193.0
55	39246.0
56	45299.0
57	54471.0
58	63643.0
59	67022.0
60	70401.0
61	71681.0
62	72961.0
63	69666.0
64	66371.0
65	59360.5
66	52350.0
67	46049.5
68	39749.0
69	35663.0
70	31577.0
71	25899.5
72	20222.0
73	16386.5
74	12551.0
75	15131.0
76	17711.0
77	11484.0
78	5257.0
79	3608.5
80	1960.0
81	1517.0
82	1074.0
83	648.5
84	223.0
85	117.5
86	12.0
87	8.5
88	5.0
89	4.0
90	3.0
91	1.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	700983.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	14.164109543312748
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.47852711304488	10.832502357403817
2	8.489444847312868	2.4049085355850286
3	2.986262186769801	1.268932342153804
4	1.245870598662477	0.7058659054499182
5	0.6858834904520184	0.48574644463560457
6	0.4612843445330755	0.392020919194902
7	0.3323664491177181	0.329537235567767
8	0.24071388284586256	0.27275982441799584
9	0.2094915800499557	0.26705355194063196
>10	5.526347594875514	22.165302154260516
>50	2.2117476432197245	21.545315649594926
>100	1.071630005640158	29.399143773814775
>500	0.03827250020143421	3.581684577229405
>1k	0.022157763274514544	6.349226728750912
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ACGTTGGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT	4912	0.7007302602202906	No Hit
ACGTTGGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC	3994	0.5697713068647884	No Hit
ACGTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	3740	0.5335364766335275	No Hit
ACGTTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTC	3690	0.5264036360368225	No Hit
ACGTTGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCC	3002	0.428255749426163	No Hit
ACGTTGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAA	2455	0.35022247329821127	No Hit
ACGTTGGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTG	2361	0.336812732976406	No Hit
ACGTTGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC	1958	0.27932203776696435	No Hit
ACGTTGGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCA	1882	0.2684801200599729	No Hit
ACGTTGGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAG	1809	0.25806617278878374	No Hit
ACGTTGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGA	1799	0.25663960466944274	No Hit
ACGTTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG	1786	0.25478506611429946	No Hit
ACGTTGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTT	1281	0.18274337608757987	No Hit
ACGTTGGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCG	1207	0.17218677200445662	No Hit
ACGTTGGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCG	1205	0.1719014583805884	No Hit
ACGTTGGGGGGGGGCTGGTGAGATGGCTCAGCGGTTAAGAACACTGACTG	1165	0.16619518590322446	No Hit
ACGTTGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGA	1088	0.1552106113842989	No Hit
ACGTTGGGGGTCCCTGTCTTACACCACAATATTTAAACTTGTCTTCCTTT	1071	0.15278544558141924	No Hit
ACGTTGGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATC	1047	0.1493616820950009	No Hit
ACGTTGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAG	1033	0.14736448672792352	No Hit
ACGTTGGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTA	1021	0.14565260498471433	No Hit
ACGTTGGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCA	1001	0.14279946874603236	No Hit
ACGTTGGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGC	855	0.12197157420365402	No Hit
ACGTTGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGC	828	0.11811984028143335	No Hit
ACGTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGT	815	0.11626530172629007	No Hit
ACGTTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTG	781	0.11141497012053074	No Hit
ACGTTGGGGGAGTTGCTTCAGTGTCCTGGTGCGGAGCGTCACGTCTCGTG	770	0.10984574518925565	No Hit
ACGTTGGGGGGGGCTGGAGAGATGGCTCAACGGTTAAGAGCACTGACTGC	762	0.10870449069378288	No Hit
ACGTTGGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGT	758	0.10813386344604649	No Hit
ACGTTGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCT	750	0.10699260895057369	No Hit
ACGTTGGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCG	747	0.10656463851477141	No Hit
ACGTTGGGGGATAGTAGAGGCCACAGGAAGACCGTCGTGGAGAAGCCCCG	744	0.10613666807896911	No Hit
ACGTTGGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCTT	742	0.10585135445510092	No Hit
ACGTTGGGGGGGCTGCCCGTGACCTGCCTGGGCGCAGGGAACGGAAAGTC	726	0.10356884546415535	No Hit
ACGTTGGGAAAAGATGGGGTTTAATGAGGTGACCTGTAGCTTTGGCACGT	725	0.10342618865222122	No Hit
ACGTTGGGGGGGGCTGGAGAGATGGCTCAGCGTTTAAGAGCACTGACTGC	706	0.10071570922547336	No Hit
ACGTTGGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTT	706	0.10071570922547336	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0019971953670773757	0.0	0.0	0.0	0.0
8	0.002282508990945572	0.0	0.0	0.0	0.0
9	0.006704870160902618	0.0	0.0	0.0	0.0
10	0.03509357573578817	0.0	0.0	0.0	0.0
11	0.06961652422383995	0.0	0.0	0.0	0.0
12	0.09215630050942747	0.0	0.0	0.0	0.0
13	0.10941777475345336	0.0	0.0	0.0	0.0
14	0.12967504204809532	0.0	0.0	0.0	0.0
15	0.15007496615467136	0.0	0.0	0.0	0.0
16	0.1724720856283248	0.0	0.0	0.0	0.0
17	0.1880216781291415	0.0	0.0	0.0	0.0
18	0.20799363179991526	0.0	0.0	0.0	0.0
19	0.23395717157192114	0.0	0.0	0.0	0.0
20	0.26091930902746574	0.0	0.0	0.0	0.0
21	0.2868828487994716	0.0	0.0	0.0	0.0
22	0.31455827031468664	0.0	0.0	0.0	0.0
23	0.3432322895134404	0.0	0.0	0.0	0.0
24	0.37518741538667844	0.0	0.0	0.0	0.0
25	0.40614394357637773	0.0	0.0	0.0	0.0
26	0.4412375193121659	0.0	0.0	0.0	0.0
27	0.4677716863319082	0.0	0.0	0.0	0.0
28	0.496588362342596	0.0	0.0	0.0	0.0
29	0.5206973635594586	0.0	0.0	0.0	0.0
30	0.5388147786750891	0.0	0.0	0.0	0.0
31	0.5602133004652039	0.0	0.0	0.0	0.0
32	0.5800425973240435	0.0	0.0	0.0	0.0
33	0.598160012439674	0.0	0.0	0.0	0.0
34	0.6122830368211497	0.0	0.0	0.0	0.0
35	0.6308284223725825	0.0	0.0	0.0	0.0
36	0.6506577192314221	0.0	0.0	0.0	0.0
37	0.6636394891174251	0.0	0.0	0.0	0.0
38	0.6776198566869668	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGAATA	30	2.526298E-6	44.0	19
TGATAGA	30	2.526298E-6	44.0	26
ACACGTA	25	4.4410575E-5	44.0	19
CATATGC	60	0.0	44.0	33
GTATTAG	20	7.8543776E-4	44.0	10
CACGGTT	20	7.8543776E-4	44.0	40
GTACACC	25	4.4410575E-5	44.0	41
CGAAATT	20	7.8543776E-4	44.0	41
CGAAAGT	25	4.4410575E-5	44.0	16
CGAAACT	75	0.0	44.0	42
CCCTATT	20	7.8543776E-4	44.0	10
GCGAAAC	20	7.8543776E-4	44.0	43
TATGGTT	25	4.4410575E-5	44.0	14
TATGGTA	20	7.8543776E-4	44.0	11
GTCCTAT	25	4.4410575E-5	44.0	17
GACGTAA	20	7.8543776E-4	44.0	9
CGCATAT	20	7.8543776E-4	44.0	41
GCTATAT	25	4.4410575E-5	44.0	20
TTCACGA	20	7.8543776E-4	44.0	37
TCTTAAC	20	7.8543776E-4	44.0	37
>>END_MODULE