FastQCFastQC Report
Fri 17 Jun 2016
SRR1527608_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527608_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences562137
Sequences flagged as poor quality0
Sequence length52
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGTTGGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG38480.6845306393281353No Hit
ACGTTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC32730.5822424071000486No Hit
ACGTTGGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT32300.5745930262551655No Hit
ACGTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC29950.5327882704749909No Hit
ACGTTGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC25320.45042400695915763No Hit
ACGTTGGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG21110.37553123171041936No Hit
ACGTTGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGA19050.3388853606860961No Hit
ACGTTGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA18510.3292791614855453No Hit
ACGTTGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGG15950.28373866157182326No Hit
ACGTTGGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAA15120.2689735776154212No Hit
ACGTTGGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC14860.26434837059293376No Hit
ACGTTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT11730.20866799374529696No Hit
ACGTTGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCT10720.1907008433887113No Hit
ACGTTGGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCG9980.177536792632401No Hit
ACGTTGGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG9900.17611365201009718No Hit
ACGTTGGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT8870.15779071649793555No Hit
ACGTTGGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAG8590.1528097243198722No Hit
ACGTTGGGGGGGGGCTGGTGAGATGGCTCAGCGGTTAAGAACACTGACTGCT8550.1520981540087203No Hit
ACGTTGGGGGTCCCTGTCTTACACCACAATATTTAAACTTGTCTTCCTTTTT8530.15174236885314435No Hit
ACGTTGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAG8390.14925187276411267No Hit
ACGTTGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC7890.14035724387471382No Hit
ACGTTGGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTG7610.13537625169665046No Hit
ACGTTGGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACA6960.12381323414043197No Hit
ACGTTGGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTC6630.1179427790734287No Hit
ACGTTGGGGGGGGCTGGAGAGATGGCTCAACGGTTAAGAGCACTGACTGCTC6440.11456282009545717No Hit
ACGTTGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCGA6400.11385124978430525No Hit
ACGTTGGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCTTGC6280.11171653885084952No Hit
ACGTTGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTTG6270.11153864627306154No Hit
ACGTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC6260.11136075369527357No Hit
ACGTTGGGGGATAGTAGAGGCCACAGGAAGACCGTCGTGGAGAAGCCCCGAT6180.10993761307296974No Hit
ACGTTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCT6060.10780290213951403No Hit
ACGTTGGGGGGGCTGCCCGTGACCTGCCTGGGCGCAGGGAACGGAAAGTCGG5950.10584608378384629No Hit
ACGTTGGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCA5940.10566819120605832No Hit
ACGTTGGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCG5870.10442294316154246No Hit
ACGTTGGGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCTTG5820.10353348027260258No Hit
ACGTTGGGGGGGGCTGGAGAGATGGCTCAGCGTTTAAGAGCACTGACTGCTC5820.10353348027260258No Hit
ACGTTGGGGAGGCTGCCTTGCCTTTGGCTGGGTGCAACTTCCATTTTAGGTG5810.10335558769481461No Hit
ACGTTGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCAT5800.10317769511702664No Hit
ACGTTGGGAAAAGATGGGGTTTAATGAGGTGACCTGTAGCTTTGGCACGTCC5780.10282190996145069No Hit
ACGTTGGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCAT5760.10246612480587472No Hit
ACGTTGGGGGCCTCATTGCGTTACTTACCTCGACTCTTAGTTGTCGGGAACG5690.1012208767613589No Hit
ACGTTGGGGGAGTTGCTTCAGTGTCCTGGTGCGGAGCGTCACGTCTCGTGCG5660.10068719902799494No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCATAT206.308688E-446.00000445
AACGTGG206.308688E-446.00000442
GATACGA206.308688E-446.0000049
ACACGTA206.308688E-446.00000419
AATTTAT206.308688E-446.00000436
ATGATAG206.308688E-446.00000442
CTACTAG206.308688E-446.00000417
ACGTTTG206.308688E-446.00000423
CCCTAAA206.308688E-446.00000428
TTTAATC405.6024874E-946.00000432
CTATAGC206.308688E-446.00000446
CTATACA206.308688E-446.00000441
GACGTAA206.308688E-446.0000049
GCTATAG206.308688E-446.00000445
GCTATAC206.308688E-446.00000440
GTAGGTC206.308688E-446.00000417
TGTATAC206.308688E-446.00000436
ATCACGA206.308688E-446.00000433
AACGGGC206.308688E-446.00000435
ACTAGGT206.308688E-446.00000444
Produced by FastQC (version 0.11.5)