##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527608_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	562137
Sequences flagged as poor quality	0
Sequence length	52
%GC	58
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.56605062466979	31.0	31.0	33.0	30.0	34.0
2	32.4300339596931	33.0	31.0	34.0	31.0	34.0
3	32.68653726760559	34.0	31.0	34.0	31.0	34.0
4	35.346134483230955	35.0	35.0	37.0	35.0	37.0
5	35.88442852898849	37.0	35.0	37.0	35.0	37.0
6	36.088658102917975	37.0	35.0	37.0	35.0	37.0
7	36.57619939623259	37.0	37.0	37.0	35.0	37.0
8	36.66989897480507	37.0	37.0	37.0	35.0	37.0
9	38.5881893559755	39.0	39.0	39.0	39.0	39.0
10	37.48788818384131	39.0	37.0	39.0	34.0	39.0
11	36.60836415322243	37.0	35.0	39.0	34.0	39.0
12	36.4387595906336	39.0	35.0	39.0	33.0	39.0
13	36.66693706338491	39.0	35.0	39.0	33.0	39.0
14	37.47248446553064	40.0	35.0	40.0	33.0	40.0
15	37.661605622828596	40.0	35.0	40.0	34.0	40.0
16	37.804351964023006	40.0	35.0	40.0	34.0	40.0
17	37.74099374351804	40.0	35.0	40.0	34.0	40.0
18	37.599585154508596	40.0	35.0	40.0	34.0	40.0
19	37.489761392685416	40.0	35.0	40.0	33.0	40.0
20	37.34876551445644	39.0	35.0	40.0	33.0	40.0
21	37.34236493950763	39.0	35.0	40.0	33.0	40.0
22	37.28891711451123	39.0	35.0	40.0	33.0	40.0
23	37.14228382049216	39.0	35.0	40.0	33.0	40.0
24	36.965170412194894	39.0	35.0	40.0	33.0	40.0
25	36.94369344127855	38.0	35.0	40.0	33.0	40.0
26	36.83397819392781	38.0	35.0	40.0	33.0	40.0
27	36.755518672494425	38.0	35.0	40.0	33.0	40.0
28	36.63066476677393	38.0	35.0	40.0	33.0	40.0
29	36.54807280075853	38.0	35.0	40.0	32.0	40.0
30	36.35885736039435	38.0	35.0	40.0	32.0	40.0
31	36.248615551013366	38.0	35.0	40.0	31.0	40.0
32	36.08030248853927	38.0	35.0	40.0	31.0	40.0
33	35.772914787676314	38.0	35.0	40.0	31.0	40.0
34	35.28847594091832	37.0	34.0	40.0	29.0	40.0
35	35.10239318884898	37.0	34.0	40.0	28.0	40.0
36	34.87186931299665	36.0	34.0	40.0	27.0	40.0
37	34.6541341345615	36.0	33.0	40.0	27.0	40.0
38	34.71491291268855	36.0	34.0	40.0	27.0	40.0
39	34.653006295618326	36.0	34.0	40.0	27.0	40.0
40	34.338364135433174	35.0	33.0	40.0	26.0	40.0
41	34.18982205405444	35.0	33.0	39.0	25.0	40.0
42	34.03117745318312	35.0	33.0	39.0	25.0	40.0
43	34.0211265225381	35.0	33.0	39.0	26.0	40.0
44	33.89519814564777	35.0	33.0	39.0	25.0	40.0
45	33.74551577284541	35.0	33.0	39.0	24.0	40.0
46	33.5583372024969	35.0	33.0	39.0	24.0	40.0
47	33.43338545585863	35.0	33.0	38.0	24.0	40.0
48	33.13419326605436	35.0	33.0	38.0	23.0	40.0
49	32.9919983918511	35.0	32.0	37.0	24.0	40.0
50	33.43472676589515	35.0	33.0	38.0	26.0	40.0
51	33.50247003844259	35.0	33.0	37.0	26.0	40.0
52	33.128600323408705	35.0	33.0	37.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	4.0
13	7.0
14	11.0
15	21.0
16	47.0
17	89.0
18	173.0
19	318.0
20	529.0
21	878.0
22	1430.0
23	2084.0
24	3070.0
25	4286.0
26	6146.0
27	8092.0
28	9136.0
29	9133.0
30	9879.0
31	12995.0
32	19725.0
33	35041.0
34	71255.0
35	49567.0
36	68066.0
37	102219.0
38	131138.0
39	16798.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	100.0	0.0	0.0
2	0.0	0.0	0.0	100.0
3	100.0	0.0	0.0	0.0
4	0.0	0.0	100.0	0.0
5	0.0	0.0	100.0	0.0
6	100.0	0.0	0.0	0.0
7	99.18845406013125	0.5601837274543394	0.16330538640936285	0.08805682600504859
8	99.21798422804406	0.5329661630527789	0.15138658369756838	0.09766302520559936
9	95.75156945726754	2.421117983694366	1.004025709035342	0.8232868500027574
10	60.59145724262947	27.732563414256667	5.027600033443805	6.648379309670063
11	35.92682922490425	28.269087428865202	17.163787475295166	18.64029587093538
12	29.37237719630624	21.86477673592025	25.037668753346605	23.725177314426908
13	23.879588071946873	19.773649484022577	28.196685149705498	28.150077294325047
14	24.511462508249767	20.23777121947141	28.626473617641253	26.62429265463757
15	25.494319000528343	20.77571837470225	26.076027729895024	27.653934894874382
16	29.55400551822776	20.68801733385278	23.02801630207583	26.72996084584363
17	31.666657772037777	21.538877533412673	20.617749765626527	26.176714928923023
18	30.74232793785145	21.140931836900968	21.420934754339243	26.695805470908336
19	30.399351047876227	21.795220738005145	19.870956724072602	27.934471490046022
20	29.97988034945218	23.262122934444807	20.438611939794036	26.31938477630898
21	31.89845180089551	21.560580427902806	21.438901904695832	25.102065866505853
22	31.734434844175	21.350667186112993	21.848054833608177	25.066843136103834
23	31.902365437606843	21.305304578777058	20.065926989328226	26.726402994287866
24	28.852041406276406	22.075579440599	22.08091621793264	26.991462935191958
25	29.17722903847283	21.897508970233233	21.820125698895467	27.105136292398473
26	28.642128164486593	21.183448162992295	21.733847798668297	28.440575873852815
27	28.95557488654901	21.050562407384675	20.939201653689405	29.05466105237691
28	31.50762180749533	22.06152592695375	19.265410389282327	27.1654418762686
29	31.286501333304873	22.724353671791754	19.343861016086823	26.645283978816554
30	29.269199501189213	22.21896085829611	19.475145738494355	29.03669390202033
31	32.05748776543796	22.88285595860084	18.827794647923906	26.231861628037294
32	34.44871979606395	21.734915154135024	19.101571325139602	24.714793724661426
33	32.87579362326266	22.818103060286017	20.02269909292575	24.283404223525583
34	30.980881884665123	22.892106372645816	23.548707877261236	22.578303865427824
35	30.344382241339744	24.298347200059773	22.065617456242872	23.29165310235761
36	31.631435041635758	24.46556622318047	18.985407471844052	24.91759126333972
37	31.779263773777565	24.403481713532464	18.652926243958323	25.164328268731644
38	33.00174156833655	24.13859966520617	19.564625705121706	23.295033061335584
39	32.46272705763897	22.77469727130575	19.40523395542368	25.3573417156316
40	31.47150961420437	21.35120086384636	21.023344842983114	26.153944678966162
41	29.092552171445753	21.856237892186424	21.14520125876788	27.906008677599946
42	28.35572111424795	19.412527551112984	22.993149356829385	29.23860197780968
43	29.112476140158005	19.10317235833969	23.667540119223606	28.116811382278694
44	27.614976420338817	18.76499856796475	24.709812732483364	28.910212279213077
45	29.427701787998302	18.715900216495267	24.548819949585244	27.30757804592119
46	29.52785530929293	20.75223655443424	22.3788862857275	27.341021850545328
47	29.782063802951946	19.93001705989821	22.944762575671053	27.343156561478786
48	30.13215639603869	20.411572267970264	23.050430766877113	26.405840569113938
49	29.194840403673837	19.418753791335565	24.699139177816086	26.687266627174516
50	27.168288157513203	20.582349142646724	24.049831268889967	28.19953143095011
51	27.75693469741362	21.271327096419558	23.22761177435394	27.744126431812887
52	30.04498903292258	20.508345830286924	22.61174767005196	26.834917466738535
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	1.5
6	3.0
7	4.5
8	6.0
9	37.5
10	69.0
11	279.0
12	489.0
13	359.0
14	321.0
15	413.0
16	451.5
17	490.0
18	600.5
19	711.0
20	728.0
21	745.0
22	844.0
23	943.0
24	1205.0
25	1467.0
26	1805.5
27	2144.0
28	2496.5
29	2849.0
30	3889.0
31	4929.0
32	5397.0
33	5865.0
34	6306.5
35	6748.0
36	6755.0
37	6762.0
38	7045.5
39	7757.5
40	8186.0
41	7678.0
42	7170.0
43	9320.5
44	11471.0
45	11552.5
46	11634.0
47	12688.5
48	13743.0
49	14843.5
50	15944.0
51	18648.0
52	21352.0
53	23464.5
54	25577.0
55	29336.5
56	33096.0
57	37804.5
58	42513.0
59	52460.0
60	62407.0
61	57238.0
62	52069.0
63	54173.0
64	48655.0
65	41033.0
66	37146.5
67	33260.0
68	29958.0
69	26656.0
70	22647.5
71	18639.0
72	16451.0
73	14263.0
74	14226.0
75	14189.0
76	10225.0
77	6261.0
78	4433.0
79	2605.0
80	1722.0
81	839.0
82	733.0
83	627.0
84	370.0
85	113.0
86	63.0
87	13.0
88	9.0
89	4.5
90	4.0
91	2.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	562137.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	12.800082542156094
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.2787614309142	9.379741949026661
2	8.618005948244711	2.2062237497264903
3	2.830975345359535	1.0871015428623272
4	1.3508630513939461	0.6916463424396544
5	0.7699363482224755	0.49276244047269613
6	0.4641854518164383	0.35649672588710585
7	0.4016454957333852	0.35987668486507735
8	0.3404953164521778	0.3486694524644348
9	0.2932429051894266	0.3378180052193682
>10	8.15521027323012	28.556917619726153
>50	2.293131723045279	19.90262160291886
>100	1.1354476471078745	26.98665983559168
>500	0.05003196486644245	4.208760497885747
>1k	0.018067098423993105	5.084703550913745
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ACGTTGGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG	3848	0.6845306393281353	No Hit
ACGTTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC	3273	0.5822424071000486	No Hit
ACGTTGGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT	3230	0.5745930262551655	No Hit
ACGTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	2995	0.5327882704749909	No Hit
ACGTTGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC	2532	0.45042400695915763	No Hit
ACGTTGGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG	2111	0.37553123171041936	No Hit
ACGTTGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGA	1905	0.3388853606860961	No Hit
ACGTTGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA	1851	0.3292791614855453	No Hit
ACGTTGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGG	1595	0.28373866157182326	No Hit
ACGTTGGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAA	1512	0.2689735776154212	No Hit
ACGTTGGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC	1486	0.26434837059293376	No Hit
ACGTTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	1173	0.20866799374529696	No Hit
ACGTTGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCT	1072	0.1907008433887113	No Hit
ACGTTGGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCG	998	0.177536792632401	No Hit
ACGTTGGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG	990	0.17611365201009718	No Hit
ACGTTGGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT	887	0.15779071649793555	No Hit
ACGTTGGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAG	859	0.1528097243198722	No Hit
ACGTTGGGGGGGGGCTGGTGAGATGGCTCAGCGGTTAAGAACACTGACTGCT	855	0.1520981540087203	No Hit
ACGTTGGGGGTCCCTGTCTTACACCACAATATTTAAACTTGTCTTCCTTTTT	853	0.15174236885314435	No Hit
ACGTTGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAG	839	0.14925187276411267	No Hit
ACGTTGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC	789	0.14035724387471382	No Hit
ACGTTGGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTG	761	0.13537625169665046	No Hit
ACGTTGGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACA	696	0.12381323414043197	No Hit
ACGTTGGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTC	663	0.1179427790734287	No Hit
ACGTTGGGGGGGGCTGGAGAGATGGCTCAACGGTTAAGAGCACTGACTGCTC	644	0.11456282009545717	No Hit
ACGTTGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCGA	640	0.11385124978430525	No Hit
ACGTTGGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCTTGC	628	0.11171653885084952	No Hit
ACGTTGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTTG	627	0.11153864627306154	No Hit
ACGTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC	626	0.11136075369527357	No Hit
ACGTTGGGGGATAGTAGAGGCCACAGGAAGACCGTCGTGGAGAAGCCCCGAT	618	0.10993761307296974	No Hit
ACGTTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCT	606	0.10780290213951403	No Hit
ACGTTGGGGGGGCTGCCCGTGACCTGCCTGGGCGCAGGGAACGGAAAGTCGG	595	0.10584608378384629	No Hit
ACGTTGGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCA	594	0.10566819120605832	No Hit
ACGTTGGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCG	587	0.10442294316154246	No Hit
ACGTTGGGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCTTG	582	0.10353348027260258	No Hit
ACGTTGGGGGGGGCTGGAGAGATGGCTCAGCGTTTAAGAGCACTGACTGCTC	582	0.10353348027260258	No Hit
ACGTTGGGGAGGCTGCCTTGCCTTTGGCTGGGTGCAACTTCCATTTTAGGTG	581	0.10335558769481461	No Hit
ACGTTGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCAT	580	0.10317769511702664	No Hit
ACGTTGGGAAAAGATGGGGTTTAATGAGGTGACCTGTAGCTTTGGCACGTCC	578	0.10282190996145069	No Hit
ACGTTGGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCAT	576	0.10246612480587472	No Hit
ACGTTGGGGGCCTCATTGCGTTACTTACCTCGACTCTTAGTTGTCGGGAACG	569	0.1012208767613589	No Hit
ACGTTGGGGGAGTTGCTTCAGTGTCCTGGTGCGGAGCGTCACGTCTCGTGCG	566	0.10068719902799494	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0012452480445158387	0.0	0.0	0.0	0.0
8	0.0014231406223038157	0.0	0.0	0.0	0.0
9	0.009072521467186824	0.0	0.0	0.0	0.0
10	0.039314259691142904	0.0	0.0	0.0	0.0
11	0.07311384947085853	0.0	0.0	0.0	0.0
12	0.09855248809453923	0.0	0.0	0.0	0.0
13	0.1211448454736123	0.0	0.0	0.0	0.0
14	0.1428477399637455	0.0	0.0	0.0	0.0
15	0.16615166765397046	0.0	0.0	0.0	0.0
16	0.19052295081092332	0.0	0.0	0.0	0.0
17	0.21009113436760077	0.0	0.0	0.0	0.0
18	0.2323277065910979	0.0	0.0	0.0	0.0
19	0.2565210971702628	0.0	0.0	0.0	0.0
20	0.2773345287714561	0.0	0.0	0.0	0.0
21	0.3033068451285007	0.0	0.0	0.0	0.0
22	0.3310580872634251	0.0	0.0	0.0	0.0
23	0.35329465948692224	0.0	0.0	0.0	0.0
24	0.3742859836659035	0.0	0.0	0.0	0.0
25	0.39492152268930886	0.0	0.0	0.0	0.0
26	0.41324445820147043	0.0	0.0	0.0	0.0
27	0.42747586442450863	0.0	0.0	0.0	0.0
28	0.447399833136762	0.0	0.0	0.0	0.0
29	0.46020809873749635	0.0	0.0	0.0	0.0
30	0.47301636433823074	0.0	0.0	0.0	0.0
31	0.4858246299389651	0.0	0.0	0.0	0.0
32	0.4979213252285475	0.0	0.0	0.0	0.0
33	0.5066380615401583	0.0	0.0	0.0	0.0
34	0.5164221533184971	0.0	0.0	0.0	0.0
35	0.5279851708747156	0.0	0.0	0.0	0.0
36	0.5383029403864182	0.0	0.0	0.0	0.0
37	0.547909139586969	0.0	0.0	0.0	0.0
38	0.5578711239430957	0.0	0.0	0.0	0.0
39	0.5694341414993143	0.0	0.0	0.0	0.0
40	0.5808192664777447	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCATAT	20	6.308688E-4	46.000004	45
AACGTGG	20	6.308688E-4	46.000004	42
GATACGA	20	6.308688E-4	46.000004	9
ACACGTA	20	6.308688E-4	46.000004	19
AATTTAT	20	6.308688E-4	46.000004	36
ATGATAG	20	6.308688E-4	46.000004	42
CTACTAG	20	6.308688E-4	46.000004	17
ACGTTTG	20	6.308688E-4	46.000004	23
CCCTAAA	20	6.308688E-4	46.000004	28
TTTAATC	40	5.6024874E-9	46.000004	32
CTATAGC	20	6.308688E-4	46.000004	46
CTATACA	20	6.308688E-4	46.000004	41
GACGTAA	20	6.308688E-4	46.000004	9
GCTATAG	20	6.308688E-4	46.000004	45
GCTATAC	20	6.308688E-4	46.000004	40
GTAGGTC	20	6.308688E-4	46.000004	17
TGTATAC	20	6.308688E-4	46.000004	36
ATCACGA	20	6.308688E-4	46.000004	33
AACGGGC	20	6.308688E-4	46.000004	35
ACTAGGT	20	6.308688E-4	46.000004	44
>>END_MODULE