FastQCFastQC Report
Fri 17 Jun 2016
SRR1527607_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527607_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences748527
Sequences flagged as poor quality0
Sequence length52
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGTTGGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG55100.7361123914033829No Hit
ACGTTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC44410.5932985717282075No Hit
ACGTTGGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT44150.5898250831299339No Hit
ACGTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC39530.528103862652917No Hit
ACGTTGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC33390.44607609344753096No Hit
ACGTTGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGA27390.36591866425659997No Hit
ACGTTGGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG24910.3327869268576818No Hit
ACGTTGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA22450.29992238088940015No Hit
ACGTTGGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC21240.2837572993358957No Hit
ACGTTGGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAA20480.2736040249717111No Hit
ACGTTGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGG19420.25944287914798No Hit
ACGTTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT15760.21054684734151205No Hit
ACGTTGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCT13580.18142298140214047No Hit
ACGTTGGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG13320.1779494928038668No Hit
ACGTTGGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT13100.17501038706686597No Hit
ACGTTGGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCG12490.16686104843245467No Hit
ACGTTGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC11520.1539022640465875No Hit
ACGTTGGGGGTCCCTGTCTTACACCACAATATTTAAACTTGTCTTCCTTTTT11450.15296709403935996No Hit
ACGTTGGGGGGGGGCTGGTGAGATGGCTCAGCGGTTAAGAACACTGACTGCT11420.1525663068934053No Hit
ACGTTGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAG11260.15042877544831382No Hit
ACGTTGGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACA10710.1430810111058118No Hit
ACGTTGGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAG10670.14254662824453893No Hit
ACGTTGGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTG9800.13092380101185397No Hit
ACGTTGGGGGATAGTAGAGGCCACAGGAAGACCGTCGTGGAGAAGCCCCGAT9170.1225072709468062No Hit
ACGTTGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCGA8740.11676265518812282No Hit
ACGTTGGGAAAAGATGGGGTTTAATGAGGTGACCTGTAGCTTTGGCACGTCC8720.11649546375748637No Hit
ACGTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC8720.11649546375748637No Hit
ACGTTGGGGGGGGCTGGAGAGATGGCTCAACGGTTAAGAGCACTGACTGCTC8480.11328916658984914No Hit
ACGTTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCT8270.11048365656816654No Hit
ACGTTGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTTG8080.1079453379771204No Hit
ACGTTGGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCG8080.1079453379771204No Hit
ACGTTGGGGGGGCTGCCCGTGACCTGCCTGGGCGCAGGGAACGGAAAGTCGG8000.10687657225457464No Hit
ACGTTGGGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCTTG7900.10554061510139247No Hit
ACGTTGGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTC7900.10554061510139247No Hit
ACGTTGGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCA7850.10487263652480137No Hit
ACGTTGGGGGAGTTGCTTCAGTGTCCTGGTGCGGAGCGTCACGTCTCGTGCG7700.10286870079502809No Hit
ACGTTGGGGTGGTTTTACCAGTGTCCATGCCAAATGTTAACTGCCAAGCTTG7580.10126555221120948No Hit
ACGTTGGGGGGGTCCCTTCGCGTCGTGCAGGGACGGATTCGGGGTGGCGGTG7530.1005975736346184No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTAT351.0189251E-746.00000431
ACACGTA351.0189251E-746.00000419
ACACCTA351.0189251E-746.00000425
TAGCGTA351.0189251E-746.00000430
TAAACGA351.0189251E-746.00000430
AAGGTAC351.0189251E-746.00000438
CTTGGTA351.0189251E-746.00000429
CGTTACC351.0189251E-746.00000438
ATATACC351.0189251E-746.00000410
GATATTC351.0189251E-746.0000049
TCCACGT351.0189251E-746.00000420
GATATAC351.0189251E-746.0000049
ATAAGGC351.0189251E-746.00000429
GTAGTAT351.0189251E-746.00000437
AACGTTA301.860526E-646.021
AATCCCG253.416006E-546.026
CAAACCG301.860526E-646.037
CGAACAA206.310037E-446.019
CATATGA206.310037E-446.021
ATTAGAT301.860526E-646.013
Produced by FastQC (version 0.11.5)