##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527607_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	748527
Sequences flagged as poor quality	0
Sequence length	52
%GC	58
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.86925388128952	31.0	30.0	31.0	26.0	33.0
2	31.273555930514195	31.0	31.0	33.0	30.0	34.0
3	31.645348798373337	31.0	31.0	34.0	30.0	34.0
4	34.67102322294319	35.0	35.0	37.0	32.0	37.0
5	35.322606933350436	35.0	35.0	37.0	35.0	37.0
6	35.598176151294474	37.0	35.0	37.0	33.0	37.0
7	36.36030497229893	37.0	35.0	37.0	35.0	37.0
8	36.49925253197279	37.0	37.0	37.0	35.0	37.0
9	38.47516789641523	39.0	39.0	39.0	37.0	39.0
10	36.925185063464646	39.0	37.0	39.0	32.0	39.0
11	35.862163956677584	37.0	35.0	39.0	31.0	39.0
12	35.63033531188588	37.0	35.0	39.0	30.0	39.0
13	35.79444161666847	37.0	35.0	39.0	31.0	39.0
14	36.68629054128976	39.0	35.0	40.0	32.0	40.0
15	36.91260969878174	39.0	35.0	40.0	33.0	40.0
16	37.02963286561474	39.0	35.0	40.0	33.0	40.0
17	37.004860212123276	39.0	35.0	40.0	33.0	40.0
18	36.9063787946193	39.0	35.0	40.0	32.0	40.0
19	36.75009852684005	39.0	35.0	40.0	32.0	40.0
20	36.56065045081874	38.0	35.0	40.0	31.0	40.0
21	36.614339896890826	38.0	35.0	40.0	31.0	40.0
22	36.555378763892286	38.0	35.0	40.0	31.0	40.0
23	36.47234769086486	38.0	35.0	40.0	31.0	40.0
24	36.348503126807714	38.0	35.0	40.0	31.0	40.0
25	36.31369476318156	38.0	35.0	40.0	31.0	40.0
26	36.17763287095856	38.0	35.0	40.0	31.0	40.0
27	36.07127331412227	38.0	35.0	40.0	31.0	40.0
28	35.873202970634324	38.0	35.0	40.0	31.0	40.0
29	35.81276293306721	38.0	34.0	40.0	30.0	40.0
30	35.66015387554491	38.0	34.0	40.0	30.0	40.0
31	35.540142172560245	37.0	34.0	40.0	30.0	40.0
32	35.40631800856883	37.0	34.0	40.0	30.0	40.0
33	35.143258693407184	37.0	34.0	40.0	29.0	40.0
34	34.77114519583128	36.0	33.0	40.0	27.0	40.0
35	34.58085813871777	36.0	33.0	39.0	27.0	40.0
36	34.43566765126709	36.0	33.0	39.0	26.0	40.0
37	34.249591531100414	35.0	33.0	39.0	26.0	40.0
38	34.072065536714106	35.0	33.0	39.0	25.0	40.0
39	33.91625686180993	35.0	33.0	39.0	25.0	40.0
40	34.10743767425891	35.0	33.0	39.0	25.0	40.0
41	34.22306476586683	35.0	33.0	39.0	26.0	40.0
42	34.25110116268351	35.0	33.0	39.0	26.0	40.0
43	34.23719652063319	35.0	33.0	39.0	27.0	40.0
44	34.13097323142652	35.0	33.0	39.0	27.0	40.0
45	34.032107058262426	35.0	33.0	39.0	27.0	40.0
46	33.75357602330978	35.0	33.0	39.0	26.0	40.0
47	33.66714627528466	35.0	33.0	38.0	25.0	40.0
48	33.473480582530755	35.0	33.0	38.0	25.0	40.0
49	33.322186106847184	35.0	33.0	37.0	25.0	40.0
50	33.20147169039995	35.0	33.0	37.0	25.0	40.0
51	33.03241833627912	35.0	33.0	37.0	24.0	40.0
52	32.509480619937555	35.0	32.0	36.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	3.0
13	9.0
14	18.0
15	28.0
16	83.0
17	171.0
18	317.0
19	598.0
20	936.0
21	1623.0
22	2321.0
23	3586.0
24	4882.0
25	7191.0
26	9434.0
27	12440.0
28	14273.0
29	15542.0
30	18350.0
31	24453.0
32	34719.0
33	55402.0
34	94598.0
35	68054.0
36	99673.0
37	133839.0
38	137249.0
39	8735.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	100.0	0.0	0.0
2	0.0	0.0	0.0	100.0
3	100.0	0.0	0.0	0.0
4	0.0	0.0	100.0	0.0
5	0.0	0.0	100.0	0.0
6	100.0	0.0	0.0	0.0
7	99.2704337986472	0.5628387486356538	0.10647578510861999	0.06025166760851646
8	99.33683086916037	0.4993807838595001	0.094852957875935	0.06893538910420065
9	95.76742054728821	2.412872214362341	0.9747143389617208	0.8449928993877309
10	60.79152789411738	27.54396301001834	4.898286902142474	6.766222193721803
11	35.82208791399642	28.169591744853562	17.303317048015636	18.705003293134382
12	29.39559962432885	21.79066352983927	24.96863840582905	23.845098440002833
13	23.718716893311797	19.555340021134842	28.29036227150123	28.43558081405213
14	24.25390132887658	20.05632395357816	28.740446236408307	26.949328481136952
15	25.479642016921233	20.394989091909842	26.36805352378738	27.757315367381537
16	29.527725786778568	20.45470637665709	23.149732741771505	26.86783509479284
17	31.55624312817039	21.331495056290557	20.689033261325243	26.423228554213807
18	30.55561122043694	20.918417104526625	21.615786738487724	26.910184936548713
19	30.421080335111494	21.394685829636074	20.022657833317968	28.161576001934467
20	29.917023701215857	22.958557273151136	20.45764548239409	26.666773543238918
21	31.871662612036705	21.261758092894446	21.592674679737673	25.273904615331176
22	31.845611447549654	21.080335111492303	21.878168723372703	25.195884717585336
23	31.782955057065408	21.085278152959077	20.186312584582787	26.94545420539273
24	28.833428854269787	21.913037205070758	22.248496046234806	27.00503789442465
25	29.105028943511723	21.757131005294397	21.85585823891456	27.281981812279316
26	28.49088944019387	21.089152428703308	21.648116901594733	28.771841229508087
27	28.860281593048747	20.96437403059609	21.07873196290849	29.096612413446678
28	31.634663813062186	21.956188621118542	19.133578347875225	27.27556921794404
29	31.353845619463293	22.782611716077042	19.32448662506496	26.539056039394705
30	29.442358124690227	22.10581582227495	19.453673681777676	28.998152371257145
31	32.16022935712406	22.740261874321167	18.677883362924785	26.42162540562999
32	34.40824445878371	21.47804955599464	19.274121040389993	24.83958494483165
33	32.75139039740718	22.758430891604444	20.139554084221412	24.35062462676697
34	30.91778920466463	22.761369997341447	23.58806028373058	22.732780514263347
35	30.05676481943871	24.299991850661364	22.19946641871302	23.4437769111869
36	31.45978702171064	24.427976545936218	19.077735338872213	25.034501093480934
37	31.407818288451857	24.56036989981657	18.71502297178325	25.316788839948323
38	33.0808374313819	24.046560778702705	19.525147389472924	23.34745440044247
39	32.17839837440734	22.86984971817984	19.365099722521702	25.58665218489113
40	31.101349717511862	21.435833309954084	21.167840305025738	26.29497666750832
41	28.732296897773896	22.043159431790706	21.31452840044514	27.91001526999026
42	28.010880035055514	19.4699723590465	23.04379133952416	29.475356266373826
43	28.862686315924474	19.296297929132816	23.71617857472075	28.124837180221956
44	27.194476618745885	18.839667774175147	24.617415270257453	29.34844033682152
45	29.198813135665112	18.81682290685573	24.46391379335682	27.520450164122334
46	29.411497514451717	20.715752404388887	22.496048906719462	27.376701174439933
47	29.390389391431437	20.238815700702848	22.827900663569917	27.542894244295795
48	29.92009640266817	20.747815376065258	22.908993262768078	26.42309495849849
49	29.035692767261565	19.660880636236236	24.60485727301754	26.698569323484655
50	27.00570587300124	20.671933009764512	23.997664746896238	28.32469637033801
51	27.632002586413044	21.305310296088184	23.011861963563103	28.050825153935666
52	30.210399892054664	20.652160843897416	22.36272038283188	26.774718881216042
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	1.0
6	2.0
7	3.5
8	5.0
9	57.5
10	110.0
11	352.5
12	595.0
13	480.0
14	488.0
15	611.0
16	641.5
17	672.0
18	803.0
19	934.0
20	1016.0
21	1098.0
22	1325.0
23	1552.0
24	1913.5
25	2275.0
26	2740.0
27	3205.0
28	3686.5
29	4168.0
30	5181.0
31	6194.0
32	6761.5
33	7329.0
34	7854.5
35	8380.0
36	8363.0
37	8346.0
38	8809.0
39	9697.0
40	10122.0
41	9754.0
42	9386.0
43	12022.0
44	14658.0
45	15092.0
46	15526.0
47	16941.0
48	18356.0
49	20236.5
50	22117.0
51	25204.0
52	28291.0
53	31246.0
54	34201.0
55	39615.5
56	45030.0
57	50878.0
58	56726.0
59	70162.5
60	83599.0
61	76622.0
62	69645.0
63	72313.0
64	64619.5
65	54258.0
66	49201.0
67	44144.0
68	39977.0
69	35810.0
70	30364.5
71	24919.0
72	22000.5
73	19082.0
74	19093.5
75	19105.0
76	13725.5
77	8346.0
78	5737.0
79	3128.0
80	2096.5
81	1065.0
82	909.0
83	753.0
84	447.0
85	141.0
86	76.5
87	12.0
88	9.0
89	6.0
90	6.0
91	3.0
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	748527.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	12.186601151327874
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.22253891690418	9.167070793705506
2	8.492655119491339	2.0699320131404746
3	3.147336110502083	1.1506598960358143
4	1.410874808156106	0.6877507424581879
5	0.7246217934663451	0.44153383912671157
6	0.46919535189651396	0.34307379693718465
7	0.30695023021267265	0.26184760202370794
8	0.2488489366367025	0.24260981901788448
9	0.22692392019294014	0.24888881763784074
>10	5.578820434115325	19.372046699718247
>50	2.718702039026529	22.957087720282633
>100	1.362639771979829	31.317774776327372
>500	0.065775049331287	5.2235924689423365
>1k	0.02302126726595045	5.780018623242715
>5k	0.0010962508221881166	0.7361123914033829
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ACGTTGGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG	5510	0.7361123914033829	No Hit
ACGTTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC	4441	0.5932985717282075	No Hit
ACGTTGGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT	4415	0.5898250831299339	No Hit
ACGTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	3953	0.528103862652917	No Hit
ACGTTGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC	3339	0.44607609344753096	No Hit
ACGTTGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGA	2739	0.36591866425659997	No Hit
ACGTTGGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG	2491	0.3327869268576818	No Hit
ACGTTGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA	2245	0.29992238088940015	No Hit
ACGTTGGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC	2124	0.2837572993358957	No Hit
ACGTTGGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAA	2048	0.2736040249717111	No Hit
ACGTTGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGG	1942	0.25944287914798	No Hit
ACGTTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	1576	0.21054684734151205	No Hit
ACGTTGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCT	1358	0.18142298140214047	No Hit
ACGTTGGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG	1332	0.1779494928038668	No Hit
ACGTTGGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT	1310	0.17501038706686597	No Hit
ACGTTGGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCG	1249	0.16686104843245467	No Hit
ACGTTGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC	1152	0.1539022640465875	No Hit
ACGTTGGGGGTCCCTGTCTTACACCACAATATTTAAACTTGTCTTCCTTTTT	1145	0.15296709403935996	No Hit
ACGTTGGGGGGGGGCTGGTGAGATGGCTCAGCGGTTAAGAACACTGACTGCT	1142	0.1525663068934053	No Hit
ACGTTGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAG	1126	0.15042877544831382	No Hit
ACGTTGGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACA	1071	0.1430810111058118	No Hit
ACGTTGGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAG	1067	0.14254662824453893	No Hit
ACGTTGGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTG	980	0.13092380101185397	No Hit
ACGTTGGGGGATAGTAGAGGCCACAGGAAGACCGTCGTGGAGAAGCCCCGAT	917	0.1225072709468062	No Hit
ACGTTGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCGA	874	0.11676265518812282	No Hit
ACGTTGGGAAAAGATGGGGTTTAATGAGGTGACCTGTAGCTTTGGCACGTCC	872	0.11649546375748637	No Hit
ACGTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC	872	0.11649546375748637	No Hit
ACGTTGGGGGGGGCTGGAGAGATGGCTCAACGGTTAAGAGCACTGACTGCTC	848	0.11328916658984914	No Hit
ACGTTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCT	827	0.11048365656816654	No Hit
ACGTTGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTTG	808	0.1079453379771204	No Hit
ACGTTGGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCG	808	0.1079453379771204	No Hit
ACGTTGGGGGGGCTGCCCGTGACCTGCCTGGGCGCAGGGAACGGAAAGTCGG	800	0.10687657225457464	No Hit
ACGTTGGGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCTTG	790	0.10554061510139247	No Hit
ACGTTGGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTC	790	0.10554061510139247	No Hit
ACGTTGGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCA	785	0.10487263652480137	No Hit
ACGTTGGGGGAGTTGCTTCAGTGTCCTGGTGCGGAGCGTCACGTCTCGTGCG	770	0.10286870079502809	No Hit
ACGTTGGGGTGGTTTTACCAGTGTCCATGCCAAATGTTAACTGCCAAGCTTG	758	0.10126555221120948	No Hit
ACGTTGGGGGGGTCCCTTCGCGTCGTGCAGGGACGGATTCGGGGTGGCGGTG	753	0.1005975736346184	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.002137531445091493	0.0	0.0	0.0	0.0
8	0.0026719143063643662	0.0	0.0	0.0	0.0
9	0.0077485514884566625	0.0	0.0	0.0	0.0
10	0.030593418807871992	0.0	0.0	0.0	0.0
11	0.060385263323834676	0.0	0.0	0.0	0.0
12	0.08162698205943139	0.0	0.0	0.0	0.0
13	0.09672329789039005	0.0	0.0	0.0	0.0
14	0.1189001866332143	0.0	0.0	0.0	0.0
15	0.14134426680667497	0.0	0.0	0.0	0.0
16	0.15871170979804336	0.0	0.0	0.0	0.0
17	0.17273925990645628	0.0	0.0	0.0	0.0
18	0.19170985148164327	0.0	0.0	0.0	0.0
19	0.21415393165510396	0.0	0.0	0.0	0.0
20	0.23379250180688205	0.0	0.0	0.0	0.0
21	0.25503422054247876	0.0	0.0	0.0	0.0
22	0.27480638640957505	0.0	0.0	0.0	0.0
23	0.2969832751523993	0.0	0.0	0.0	0.0
24	0.31849218531863244	0.0	0.0	0.0	0.0
25	0.3400010954848656	0.0	0.0	0.0	0.0
26	0.361643601366417	0.0	0.0	0.0	0.0
27	0.3798126186496947	0.0	0.0	0.0	0.0
28	0.39931759308615455	0.0	0.0	0.0	0.0
29	0.4118755903260671	0.0	0.0	0.0	0.0
30	0.4252351618578889	0.0	0.0	0.0	0.0
31	0.4377931590978014	0.0	0.0	0.0	0.0
32	0.4520879006368508	0.0	0.0	0.0	0.0
33	0.46504668502271795	0.0	0.0	0.0	0.0
34	0.4746655765256297	0.0	0.0	0.0	0.0
35	0.49029627521786123	0.0	0.0	0.0	0.0
36	0.5024534853118191	0.0	0.0	0.0	0.0
37	0.5115379939534579	0.0	0.0	0.0	0.0
38	0.518351375434687	0.0	0.0	0.0	0.0
39	0.5266343097844166	0.0	0.0	0.0	0.0
40	0.5358524141413736	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGCGTAT	35	1.0189251E-7	46.000004	31
ACACGTA	35	1.0189251E-7	46.000004	19
ACACCTA	35	1.0189251E-7	46.000004	25
TAGCGTA	35	1.0189251E-7	46.000004	30
TAAACGA	35	1.0189251E-7	46.000004	30
AAGGTAC	35	1.0189251E-7	46.000004	38
CTTGGTA	35	1.0189251E-7	46.000004	29
CGTTACC	35	1.0189251E-7	46.000004	38
ATATACC	35	1.0189251E-7	46.000004	10
GATATTC	35	1.0189251E-7	46.000004	9
TCCACGT	35	1.0189251E-7	46.000004	20
GATATAC	35	1.0189251E-7	46.000004	9
ATAAGGC	35	1.0189251E-7	46.000004	29
GTAGTAT	35	1.0189251E-7	46.000004	37
AACGTTA	30	1.860526E-6	46.0	21
AATCCCG	25	3.416006E-5	46.0	26
CAAACCG	30	1.860526E-6	46.0	37
CGAACAA	20	6.310037E-4	46.0	19
CATATGA	20	6.310037E-4	46.0	21
ATTAGAT	30	1.860526E-6	46.0	13
>>END_MODULE