FastQCFastQC Report
Fri 17 Jun 2016
SRR1527606_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527606_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences566504
Sequences flagged as poor quality0
Sequence length50
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTACCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA54270.9579808792170929No Hit
TCTACCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA39540.6979650629121772No Hit
TCTACCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC36160.6383008769576207No Hit
TCTACCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA29420.5193255475689492No Hit
TCTACCGGGGGGTGACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCT24310.4291231835962323No Hit
TCTACCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG20950.3698120401621171No Hit
TCTACCGGGACCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA19620.34633471255277987No Hit
TCTACCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTCTACCGGGA16260.28702356911866467No Hit
TCTACCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT15060.26584101789219494No Hit
TCTACCGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGC13920.24571759422704872No Hit
TCTACCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT11790.208118565800065No Hit
TCTACCGGGGTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11590.20458814059565333No Hit
TCTACCGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCG11510.20317597051388872No Hit
TCTACCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC10520.18570036575205118No Hit
TCTACCGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCA10430.18411167441006593No Hit
TCTACCGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10210.18022820668521317No Hit
TCTACCGGGAGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9800.17299083501616935No Hit
TCTACCGGGATACCAGCCACGTCCAGGAAGAGCAGACAGAGGTCGAGGAG9490.16751867594933134No Hit
TCTACCGGGCTGATCATAGCAAACACCCTCTTCCAACAACACAAGAGAAG9150.16151695310183156No Hit
TCTACCGGGCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGC8950.15798652789741996No Hit
TCTACCGGGATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8520.150396113707935No Hit
TCTACCGGGATTTCTGGTGGTCTCAGGCAATCTTGGAGAATTTGCATAGG8460.1493369861466115No Hit
TCTACCGGGATTTCTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8330.14704220976374394No Hit
TCTACCGGGGGTGACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTG8300.1465126459830822No Hit
TCTACCGGGAGATTAATTTGTTTTATAATGTTTATATGCTATTTAGAATG8270.14598308220242046No Hit
TCTACCGAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8190.14457091212065581No Hit
TCTACCGGGGAGTTCCCTGGGTCCTGGAGGGTGCAGGGTTTGTGGAACAG8020.14157005069690592No Hit
TCTACCGGGGTACCAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7790.13751006171183258No Hit
TCTACCGGGGATGGTAGTCGCCGTGCCTACCATGGTGACCACGGGTGACG7730.13645093415050907No Hit
TCTACCGGATCACAGACAAGGAAAAGGCAGAGAAGCTGAAGCAGTCCCTA7680.1355683278494062No Hit
TCTACCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTTC7430.13115529634389167No Hit
TCTACCGGGAGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7390.13044921130300932No Hit
TCTACCGGGATGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7180.12674226483837714No Hit
TCTACCGGGGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7170.12656574357815656No Hit
TCTACCGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACA7160.12638922231793598No Hit
TCTACCGGGGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCC6990.1233883608941861No Hit
TCTACCGGGGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6960.12285879711352436No Hit
TCTACCGGGGCTCTGGCTGGGCACAGTGGCTACAGCAGTCATTCCTTCAG6850.12091706325109797No Hit
TCTACCGGGAGAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGA6750.11915185064889215No Hit
TCTACCGGGCACTCTGGAAGAAATTTGGACCAAAAAACTGTTTGTGACAC6730.118798808128451No Hit
TCTACCGGGATTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6690.11809272308756866No Hit
TCTACCGGGTGAGGTTTGGAATGACAAAGACAGGAGAGGAGATGCTGGAG6620.11685707426602461No Hit
TCTACCGGGGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGG6610.11668055300580402No Hit
TCTACCGGGATTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6600.11650403174558344No Hit
TCTACCGGGAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6530.11526838292403936No Hit
TCTACCGGGGCTACTCAACAGGAATTGATTCTGTTGTCAGGGAATTGGCC6410.1131501278013924No Hit
TCTACCGGGACTCTCTTTGTGGAGAAGGAACGAGATAAGGAAGTCAGTGA6400.11297360654117182No Hit
TCTACCGAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6340.11191447897984834No Hit
TCTACCGGGATTTCCGCCACTGGCATATTCAGTCTTTTGTATTTTTGATT6260.1105023088980837No Hit
TCTACCGGGCACTGCCCAGCTCCAAAGCTTCCTTCCTGGCTTTCCAGTCT6170.10891361755609845No Hit
TCTACCGGGAGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6070.10714840495389265No Hit
TCTACCGGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6020.10626579865278973No Hit
TCTACCGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5950.10503014983124569No Hit
TCTACCGGGAGGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGC5930.10467710731080451No Hit
TCTACCGGGAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCG5920.10450058605058392No Hit
TCTACCGGGATGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTCCGG5880.10379450100970161No Hit
TCTACCGGGAAACATTCTTCCCTCAACATTTCCTGGGCCTTTCAGGAATA5750.10149972462683404No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACAT650.044.00000411
CGGTGCC650.044.0000046
TCATAGC1300.044.00000414
GAACACC650.044.00000432
AGTCGTC650.044.00000416
TGCCGAG650.044.00000411
TGATCGA254.4399712E-544.010
CGGACAG351.4443503E-744.06
TCACGAC408.300049E-944.020
GCCCCGC207.853098E-444.011
CTTAACG600.044.027
ATCCTAG254.4399712E-544.034
CTATGTG351.4443503E-744.042
CTTCGAA254.4399712E-544.026
ACTATTC302.525434E-644.026
CGGGTAC1900.044.06
AAGTACG254.4399712E-544.09
TTAGGCA207.853098E-444.027
CAGAACG551.8189894E-1244.018
CTATGAG254.4399712E-544.024
Produced by FastQC (version 0.11.5)