##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1527606_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 566504 Sequences flagged as poor quality 0 Sequence length 50 %GC 39 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.528082767288492 31.0 31.0 33.0 30.0 34.0 2 31.772077514015788 31.0 31.0 34.0 30.0 34.0 3 30.844655995368083 31.0 31.0 31.0 30.0 34.0 4 35.57251846412382 37.0 35.0 37.0 35.0 37.0 5 35.93543734907432 37.0 35.0 37.0 35.0 37.0 6 36.28750900258427 37.0 36.0 37.0 35.0 37.0 7 36.44724485617048 37.0 37.0 37.0 35.0 37.0 8 36.62982609125443 37.0 37.0 37.0 35.0 37.0 9 38.366256901981274 39.0 39.0 39.0 37.0 39.0 10 36.46485461709008 39.0 35.0 39.0 31.0 39.0 11 36.97898690918334 39.0 37.0 39.0 34.0 39.0 12 36.98691624419245 39.0 37.0 39.0 34.0 39.0 13 37.12384908138336 39.0 37.0 39.0 34.0 39.0 14 37.970663931763944 40.0 38.0 40.0 34.0 40.0 15 38.01510139381187 40.0 38.0 40.0 34.0 40.0 16 38.127822574950926 40.0 38.0 40.0 35.0 40.0 17 38.04640390888679 40.0 38.0 40.0 34.0 40.0 18 37.90159822349004 40.0 38.0 40.0 34.0 40.0 19 37.83664899100447 40.0 37.0 40.0 34.0 40.0 20 37.70791556635081 39.0 37.0 40.0 33.0 40.0 21 37.63349420304181 39.0 37.0 40.0 33.0 40.0 22 37.50375813763009 39.0 36.0 40.0 33.0 40.0 23 37.212040868202166 38.0 36.0 40.0 33.0 40.0 24 37.092968452120374 38.0 35.0 40.0 33.0 40.0 25 36.844571265163175 38.0 35.0 40.0 33.0 40.0 26 36.180973126403345 38.0 35.0 40.0 31.0 40.0 27 35.88782956519283 37.0 35.0 39.0 31.0 40.0 28 35.56318755030856 37.0 34.0 39.0 31.0 40.0 29 35.134465069973025 36.0 34.0 38.0 30.0 40.0 30 34.669522192252835 35.0 34.0 38.0 30.0 40.0 31 34.63744121842035 35.0 33.0 38.0 30.0 40.0 32 34.359217234123676 35.0 33.0 38.0 29.0 39.0 33 33.979654159546975 35.0 33.0 38.0 28.0 39.0 34 33.5522555886631 35.0 33.0 37.0 27.0 39.0 35 33.49778642339683 35.0 33.0 37.0 27.0 39.0 36 33.25402291952043 35.0 33.0 36.0 26.0 38.0 37 32.95208153870052 35.0 32.0 36.0 26.0 38.0 38 32.60167977631226 35.0 31.0 36.0 24.0 38.0 39 31.819478768022822 34.0 31.0 35.0 23.0 38.0 40 31.62631331817604 34.0 30.0 35.0 22.0 38.0 41 31.192016296442745 34.0 30.0 35.0 21.0 37.0 42 30.996656687331424 33.0 29.0 35.0 21.0 37.0 43 30.64747115642608 33.0 29.0 35.0 21.0 37.0 44 30.252444819454055 33.0 28.0 35.0 20.0 36.0 45 29.961177679239686 33.0 27.0 35.0 20.0 36.0 46 29.846712468049653 33.0 27.0 35.0 20.0 36.0 47 29.5499378645164 32.0 26.0 35.0 20.0 35.0 48 29.083589877564854 32.0 26.0 35.0 18.0 35.0 49 28.311304068462004 31.0 24.0 34.0 17.0 35.0 50 30.07780880629263 33.0 27.0 35.0 20.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 2.0 12 3.0 13 6.0 14 19.0 15 36.0 16 71.0 17 131.0 18 190.0 19 280.0 20 475.0 21 736.0 22 1254.0 23 1891.0 24 2805.0 25 3995.0 26 5755.0 27 7632.0 28 10108.0 29 12918.0 30 16611.0 31 21288.0 32 30860.0 33 46887.0 34 66277.0 35 133185.0 36 173648.0 37 29033.0 38 408.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 0.0 100.0 0.0 2 0.0 0.0 0.0 100.0 3 0.0 0.0 100.0 0.0 4 0.0 100.0 0.0 0.0 5 0.0 0.0 0.0 100.0 6 0.0 0.0 0.0 100.0 7 99.16734921553953 0.38711112366373407 0.06919633400646774 0.37634332679027865 8 98.43054947537881 1.2021097821021565 0.16734215468911076 0.19999858782991822 9 91.9993504017624 5.485398161354554 1.2605383192351687 1.2547131176478896 10 40.88603081355118 46.279284877070594 6.581948229844803 6.2527360795334195 11 31.126523378475703 26.48118989451089 28.64304576843235 13.749240958581051 12 28.789205371894994 24.251373335404516 25.556924575995932 21.40249671670456 13 22.84255715758406 26.268128733424657 28.525129566605003 22.364184542386283 14 20.678229986019517 33.13198141584172 25.842359453772612 20.347429144366146 15 22.638145538248626 36.00327623458969 23.495332777879767 17.863245449281912 16 24.397356417606936 39.08569048056148 21.586784912374846 14.930168189456738 17 23.73293039413667 40.48285625520738 20.105771539124174 15.678441811531782 18 22.46656687331422 42.365808537980314 20.27452586389505 14.893098724810416 19 20.241516388233798 45.41627243585217 19.57073559939559 14.771475576518437 20 20.25387287644924 46.94953610212814 18.113552596274694 14.683038425147924 21 17.89678448872382 47.28139607134283 21.725707144168442 13.096112295764902 22 16.747278042167398 46.5126459830822 19.75873074153051 16.981345233219887 23 16.66607826246593 49.68084956152119 19.937370256873738 13.715701919139143 24 16.238896812732126 50.8646011325604 17.65071385197633 15.245788202731136 25 16.226363803256465 51.59663479869515 16.373406013020208 15.803595385028174 26 18.26094784855888 50.1586926129383 16.66537217742505 14.91498736107777 27 14.950115091861663 49.89408724386765 17.303496533122452 17.852301131148234 28 16.11250759041419 51.91172524818889 17.223355880982304 14.752411280414613 29 19.779030686455876 49.539809074604946 17.18822815019841 13.492932088740767 30 17.84223941931566 49.34934263482694 17.994400745625803 14.814017200231596 31 19.838694872410436 48.73257735161623 16.844011692768277 14.584716083205059 32 19.28741897674156 49.39982771525002 17.875954980017795 13.436798327990623 33 19.230755652210753 50.225594170561905 17.964745173908746 12.5789050033186 34 14.9795941423185 51.35744849109627 19.5673816954514 14.095575671133831 35 14.865561408216005 53.87728948074506 18.757502153559376 12.499646957479559 36 16.818945673816955 53.9376597517405 16.052313840678973 13.191080733763574 37 17.5389758942567 53.02063180489458 16.5834663126827 12.856925988166015 38 19.693064832728453 53.226102551791335 14.63890811009278 12.44192450538743 39 18.0124059141683 52.38250744919718 14.449147755355654 15.15593888127886 40 16.44119017694491 52.28294945843277 16.876844647169307 14.39901571745301 41 14.176069365794417 53.64145707709036 16.36510951378984 15.817364043325377 42 15.238197788541651 51.826995043283006 16.47720051402991 16.457606654145426 43 15.756111166028836 52.420459520144604 15.962464519226696 15.86096479459986 44 15.950284552271476 53.34560744496068 15.778882408597292 14.925225594170563 45 17.48531343114965 52.01499018541793 15.062029570841512 15.437666812590908 46 15.576236001864064 52.85434877776679 16.014891333512207 15.554523886856932 47 17.256365356643556 53.7498411308658 15.636253230339062 13.35754028215158 48 16.588055865448435 53.70182734808581 15.341815768291134 14.368301018174629 49 17.07860844760143 53.0199257198537 15.345875757276206 14.555590075268665 50 17.51037945010097 51.47359948032141 16.00659483428184 15.009426235295779 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 13.5 6 27.0 7 57.0 8 87.0 9 362.5 10 638.0 11 5275.5 12 9913.0 13 19823.5 14 29734.0 15 36553.0 16 43372.0 17 36124.5 18 28877.0 19 26508.0 20 24139.0 21 21338.0 22 18537.0 23 16691.5 24 14846.0 25 13464.0 26 12082.0 27 11785.5 28 11489.0 29 11184.5 30 10880.0 31 11388.5 32 11897.0 33 13674.0 34 15451.0 35 15506.0 36 15561.0 37 14665.5 38 13770.0 39 14778.5 40 15787.0 41 15172.5 42 14558.0 43 15529.5 44 16501.0 45 17293.0 46 18085.0 47 20000.0 48 21915.0 49 21853.5 50 21792.0 51 21512.5 52 21233.0 53 22397.0 54 23561.0 55 24559.5 56 25558.0 57 25089.0 58 24620.0 59 26035.0 60 27450.0 61 25772.0 62 24094.0 63 25481.5 64 26869.0 65 18197.0 66 9525.0 67 7506.5 68 5488.0 69 4484.5 70 3481.0 71 2714.0 72 1947.0 73 1421.0 74 895.0 75 916.0 76 937.0 77 678.0 78 419.0 79 283.0 80 147.0 81 240.5 82 334.0 83 168.5 84 3.0 85 2.5 86 2.0 87 1.5 88 1.0 89 1.0 90 1.0 91 1.0 92 1.0 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 50 566504.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 15.060087836979086 #Duplication Level Percentage of deduplicated Percentage of total 1 77.8751933986591 11.7280725290554 2 7.204979136387078 2.1701523731518226 3 2.328988700829856 1.0522432321748831 4 1.1392939190773126 0.6863146597376188 5 0.6985793989404098 0.5260333554573312 6 0.4747057996155469 0.42894666233601175 7 0.37624830043602603 0.39664327171564545 8 0.3750761873505556 0.4518944261646873 9 0.31998687233344275 0.4337127363619674 >10 6.839279853720287 23.948815895386442 >50 1.1896947817525434 12.494880883453604 >100 1.0959257349149047 33.34574866196885 >500 0.06329410661540626 6.4131939050739275 >1k 0.01758169628205729 4.965366528744722 >5k 0.0011721130854704862 0.9579808792170929 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TCTACCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5427 0.9579808792170929 No Hit TCTACCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3954 0.6979650629121772 No Hit TCTACCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC 3616 0.6383008769576207 No Hit TCTACCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2942 0.5193255475689492 No Hit TCTACCGGGGGGTGACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCT 2431 0.4291231835962323 No Hit TCTACCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG 2095 0.3698120401621171 No Hit TCTACCGGGACCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1962 0.34633471255277987 No Hit TCTACCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTCTACCGGGA 1626 0.28702356911866467 No Hit TCTACCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT 1506 0.26584101789219494 No Hit TCTACCGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGC 1392 0.24571759422704872 No Hit TCTACCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT 1179 0.208118565800065 No Hit TCTACCGGGGTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1159 0.20458814059565333 No Hit TCTACCGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCG 1151 0.20317597051388872 No Hit TCTACCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC 1052 0.18570036575205118 No Hit TCTACCGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCA 1043 0.18411167441006593 No Hit TCTACCGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1021 0.18022820668521317 No Hit TCTACCGGGAGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 980 0.17299083501616935 No Hit TCTACCGGGATACCAGCCACGTCCAGGAAGAGCAGACAGAGGTCGAGGAG 949 0.16751867594933134 No Hit TCTACCGGGCTGATCATAGCAAACACCCTCTTCCAACAACACAAGAGAAG 915 0.16151695310183156 No Hit TCTACCGGGCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGC 895 0.15798652789741996 No Hit TCTACCGGGATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 852 0.150396113707935 No Hit TCTACCGGGATTTCTGGTGGTCTCAGGCAATCTTGGAGAATTTGCATAGG 846 0.1493369861466115 No Hit TCTACCGGGATTTCTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 833 0.14704220976374394 No Hit TCTACCGGGGGTGACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTG 830 0.1465126459830822 No Hit TCTACCGGGAGATTAATTTGTTTTATAATGTTTATATGCTATTTAGAATG 827 0.14598308220242046 No Hit TCTACCGAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 819 0.14457091212065581 No Hit TCTACCGGGGAGTTCCCTGGGTCCTGGAGGGTGCAGGGTTTGTGGAACAG 802 0.14157005069690592 No Hit TCTACCGGGGTACCAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 779 0.13751006171183258 No Hit TCTACCGGGGATGGTAGTCGCCGTGCCTACCATGGTGACCACGGGTGACG 773 0.13645093415050907 No Hit TCTACCGGATCACAGACAAGGAAAAGGCAGAGAAGCTGAAGCAGTCCCTA 768 0.1355683278494062 No Hit TCTACCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTTC 743 0.13115529634389167 No Hit TCTACCGGGAGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 739 0.13044921130300932 No Hit TCTACCGGGATGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 718 0.12674226483837714 No Hit TCTACCGGGGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 717 0.12656574357815656 No Hit TCTACCGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACA 716 0.12638922231793598 No Hit TCTACCGGGGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCC 699 0.1233883608941861 No Hit TCTACCGGGGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 696 0.12285879711352436 No Hit TCTACCGGGGCTCTGGCTGGGCACAGTGGCTACAGCAGTCATTCCTTCAG 685 0.12091706325109797 No Hit TCTACCGGGAGAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGA 675 0.11915185064889215 No Hit TCTACCGGGCACTCTGGAAGAAATTTGGACCAAAAAACTGTTTGTGACAC 673 0.118798808128451 No Hit TCTACCGGGATTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 669 0.11809272308756866 No Hit TCTACCGGGTGAGGTTTGGAATGACAAAGACAGGAGAGGAGATGCTGGAG 662 0.11685707426602461 No Hit TCTACCGGGGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGG 661 0.11668055300580402 No Hit TCTACCGGGATTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 660 0.11650403174558344 No Hit TCTACCGGGAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 653 0.11526838292403936 No Hit TCTACCGGGGCTACTCAACAGGAATTGATTCTGTTGTCAGGGAATTGGCC 641 0.1131501278013924 No Hit TCTACCGGGACTCTCTTTGTGGAGAAGGAACGAGATAAGGAAGTCAGTGA 640 0.11297360654117182 No Hit TCTACCGAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 634 0.11191447897984834 No Hit TCTACCGGGATTTCCGCCACTGGCATATTCAGTCTTTTGTATTTTTGATT 626 0.1105023088980837 No Hit TCTACCGGGCACTGCCCAGCTCCAAAGCTTCCTTCCTGGCTTTCCAGTCT 617 0.10891361755609845 No Hit TCTACCGGGAGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 607 0.10714840495389265 No Hit TCTACCGGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 602 0.10626579865278973 No Hit TCTACCGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 595 0.10503014983124569 No Hit TCTACCGGGAGGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGC 593 0.10467710731080451 No Hit TCTACCGGGAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCG 592 0.10450058605058392 No Hit TCTACCGGGATGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTCCGG 588 0.10379450100970161 No Hit TCTACCGGGAAACATTCTTCCCTCAACATTTCCTGGGCCTTTCAGGAATA 575 0.10149972462683404 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0010591275613234857 0.0 0.0 0.0 0.0 8 0.0033539039441910384 0.0 0.0 0.0 0.0 9 0.004766074025955686 0.0 0.0 0.0 0.0 10 0.01676951972095519 0.0 0.0 0.0 0.0 11 0.031773826839704575 0.0 0.0 0.0 0.0 12 0.05454506940815952 0.0 0.0 0.0 0.0 13 0.07113806786889414 0.0 0.0 0.0 0.0 14 0.10467710731080451 0.0 0.0 0.0 0.0 15 0.14104048691624418 0.0 0.0 0.0 0.0 16 0.17881603660344852 0.0 0.0 0.0 0.0 17 0.20705943823874148 0.0 0.0 0.0 0.0 18 0.23124285088896107 0.0 0.0 0.0 0.0 19 0.26107494386623925 0.0 0.0 0.0 0.0 20 0.2872000903788852 0.0 0.0 0.0 0.0 21 0.31191306680976655 0.0 0.0 0.0 0.0 22 0.3345077881180009 0.0 0.0 0.0 0.0 23 0.3616920621919704 0.0 0.0 0.0 0.0 24 0.3895824213068222 0.0 0.0 0.0 0.0 25 0.4255927583918207 0.0 0.0 0.0 0.0 26 0.4656630844618926 0.0 0.0 0.0 0.0 27 0.5165012074054199 0.0 0.0 0.0 0.0 28 0.6014079335715193 0.0 0.0 0.0 0.0 29 0.6481860675299733 0.0 0.0 0.0 0.0 30 0.7046728708005592 0.0 0.0 0.0 0.0 31 0.7491562283761456 0.0 0.0 0.0 0.0 32 0.7996413087992318 0.0 0.0 0.0 0.0 33 0.8469490065383475 0.0 0.0 0.0 0.0 34 0.9020236397271687 0.0 0.0 0.0 0.0 35 0.9699843249120924 0.0 0.0 0.0 0.0 36 1.013938118707017 0.0 0.0 0.0 0.0 37 1.0527727959555448 0.0 0.0 0.0 0.0 38 1.0926666007653962 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGACAT 65 0.0 44.000004 11 CGGTGCC 65 0.0 44.000004 6 TCATAGC 130 0.0 44.000004 14 GAACACC 65 0.0 44.000004 32 AGTCGTC 65 0.0 44.000004 16 TGCCGAG 65 0.0 44.000004 11 TGATCGA 25 4.4399712E-5 44.0 10 CGGACAG 35 1.4443503E-7 44.0 6 TCACGAC 40 8.300049E-9 44.0 20 GCCCCGC 20 7.853098E-4 44.0 11 CTTAACG 60 0.0 44.0 27 ATCCTAG 25 4.4399712E-5 44.0 34 CTATGTG 35 1.4443503E-7 44.0 42 CTTCGAA 25 4.4399712E-5 44.0 26 ACTATTC 30 2.525434E-6 44.0 26 CGGGTAC 190 0.0 44.0 6 AAGTACG 25 4.4399712E-5 44.0 9 TTAGGCA 20 7.853098E-4 44.0 27 CAGAACG 55 1.8189894E-12 44.0 18 CTATGAG 25 4.4399712E-5 44.0 24 >>END_MODULE