FastQCFastQC Report
Fri 17 Jun 2016
SRR1527605_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527605_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences460592
Sequences flagged as poor quality0
Sequence length52
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTACCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA45620.9904644457567653No Hit
TCTACCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA32770.7114756660992809No Hit
TCTACCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC30670.6658821690346337No Hit
TCTACCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA23740.5154236287212978No Hit
TCTACCGGGGGGTGACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGA20070.4357435648035572No Hit
TCTACCGGGACCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA17050.3701757737867788No Hit
TCTACCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT17000.3690902143328586No Hit
TCTACCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC12880.2796401153298364No Hit
TCTACCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTCTACCGGGAAG12000.26053426894084136No Hit
TCTACCGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTC11390.24729044360301528No Hit
TCTACCGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCGCC10180.2210199048181471No Hit
TCTACCGGGGTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10050.21819745023795464No Hit
TCTACCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT9880.21450654809462605No Hit
TCTACCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA9500.20625629624483272No Hit
TCTACCGGGATACCAGCCACGTCCAGGAAGAGCAGACAGAGGTCGAGGAGAC8790.1908413519991663No Hit
TCTACCGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8470.18389377149407718No Hit
TCTACCGGGATTTCTGGTGGTCTCAGGCAATCTTGGAGAATTTGCATAGGGG8470.18389377149407718No Hit
TCTACCGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACA8250.17911730989682845No Hit
TCTACCGGGCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCAT7650.16609059644978635No Hit
TCTACCGGGAGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7570.1643537013235141No Hit
TCTACCGGGATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7410.16087991107096952No Hit
TCTACCGGGCTGATCATAGCAAACACCCTCTTCCAACAACACAAGAGAAGGC7250.15740612081842498No Hit
TCTACCGGGGGTGACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAG7170.15566922569215272No Hit
TCTACCGGGGAGTTCCCTGGGTCCTGGAGGGTGCAGGGTTTGTGGAACAGTA7020.1524125473303922No Hit
TCTACCGGATCACAGACAAGGAAAAGGCAGAGAAGCTGAAGCAGTCCCTACC7010.15219543543960815No Hit
TCTACCGGGGATGGTAGTCGCCGTGCCTACCATGGTGACCACGGGTGACGGG6970.151326987876472No Hit
TCTACCGAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6830.14828742140549553No Hit
TCTACCGGGGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTA6480.14068850522805432No Hit
TCTACCGGGAGATTAATTTGTTTTATAATGTTTATATGCTATTTAGAATGTT6350.1378660506478619No Hit
TCTACCGGGAGAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA6350.1378660506478619No Hit
TCTACCGGGGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6240.13547781984923749No Hit
TCTACCGGGATTTCTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6200.13460937228610137No Hit
TCTACCGGGGTACCAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6120.1328724771598291No Hit
TCTACCGGGGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5990.13005002257963663No Hit
TCTACCGGGAGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5970.1296157987980686No Hit
TCTACCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTTCTC5960.12939868690728454No Hit
TCTACCGGGTGAGGTTTGGAATGACAAAGACAGGAGAGGAGATGCTGGAGCG5950.1291815750165005No Hit
TCTACCGGGGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGGCA5870.12744467989022823No Hit
TCTACCGGGATGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5830.1265762323270921No Hit
TCTACCGGGGCTACTCAACAGGAATTGATTCTGTTGTCAGGGAATTGGCCGT5790.12570778476395594No Hit
TCTACCGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAAC5720.1241880015284677No Hit
TCTACCGGGATGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTCCGGAG5680.12331955396533158No Hit
TCTACCGGGAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5520.11984576371278702No Hit
TCTACCGGGCACTCTGGAAGAAATTTGGACCAAAAAACTGTTTGTGACACCA5500.11941153993121896No Hit
TCTACCGGGATTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5320.11550352589710633No Hit
TCTACCGGGAGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5300.11506930211553827No Hit
TCTACCGGGATTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5290.11485219022475424No Hit
TCTACCGGGGCTCTGGCTGGGCACAGTGGCTACAGCAGTCATTCCTTCAGTT5190.11268107131691388No Hit
TCTACCGGGAGGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTA5150.11181262375377775No Hit
TCTACCGGGGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGG5120.11116128808142565No Hit
TCTACCGGGATTTCCGCCACTGGCATATTCAGTCTTTTGTATTTTTGATTAT5020.1089901691735853No Hit
TCTACCGAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5010.10877305728280127No Hit
TCTACCGGGAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTT4950.10747038593809707No Hit
TCTACCGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4940.10725327404731302No Hit
TCTACCGGGACTCTCTTTGTGGAGAAGGAACGAGATAAGGAAGTCAGTGATG4830.10486504324868864No Hit
TCTACCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC4770.10356237190398444No Hit
TCTACCGGGGTCGCATAGGGGTGGCACTGCTAATGAGGGTTTGCATGACTGC4740.10291103623163234No Hit
TCTACCGGGAGCCACTGGCCCATTTGACCGAGAACACCTGCTCATGTACCTG4730.1026939243408483No Hit
TCTACCGGGGGGGTTGGGGATTTAGCTCAGTGGTAAAGTGCTTGCCTAGCAA4710.10225970055928024No Hit
TCTACCGGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4700.10204258866849619No Hit
TCTACCGGGCACTGCCCAGCTCCAAAGCTTCCTTCCTGGCTTTCCAGTCTGA4650.10095702921457603No Hit
TCTACCGGGGAGGCTACGAAAGCTGAGGAGGCCAAGGATGAGCCCCCCTCTG4620.10030569354222392No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTCA1100.046.0000046
CGGGTAC1450.046.0000046
CGGAACG551.8189894E-1246.0000046
ATCTAGC351.01777914E-746.00000415
TAGACGT351.01777914E-746.00000432
GGGTCGA351.01777914E-746.0000048
GCGCGAC850.046.0000049
GATCCGT351.01777914E-746.00000431
CCGGAGT1450.046.0000045
TCGGGGC551.8189894E-1246.00000412
CCTACGA351.01777914E-746.00000438
GCTGCCG351.01777914E-746.0000049
GCGCCCT351.01777914E-746.00000446
TTCGTGC351.01777914E-746.00000416
CTCCACG700.046.00000411
CCGCTTC351.01777914E-746.00000442
GCGCACC351.01777914E-746.00000416
GCGCAAG351.01777914E-746.00000433
GCGCAAC351.01777914E-746.00000435
ATCCGTA351.01777914E-746.00000432
Produced by FastQC (version 0.11.5)