FastQCFastQC Report
Fri 17 Jun 2016
SRR1527604_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527604_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences592675
Sequences flagged as poor quality0
Sequence length52
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTACCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA56950.9608976251739991No Hit
TCTACCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA39550.6673134517231197No Hit
TCTACCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC38110.6430168304720125No Hit
TCTACCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA29270.4938625722360484No Hit
TCTACCGGGGGGTGACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGA26260.44307588475977555No Hit
TCTACCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT20980.3539882735057156No Hit
TCTACCGGGACCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA19820.33441599527565696No Hit
TCTACCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTCTACCGGGAAG17610.297127430716666No Hit
TCTACCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC15800.2665879276163159No Hit
TCTACCGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTC14430.24347239212047073No Hit
TCTACCGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCGCC13690.23098662842198506No Hit
TCTACCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT13520.2281182773020627No Hit
TCTACCGGGGTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13330.22491247310920826No Hit
TCTACCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA12120.2044965621968195No Hit
TCTACCGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACA11550.1948791496182562No Hit
TCTACCGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11030.18610536972202302No Hit
TCTACCGGGCTGATCATAGCAAACACCCTCTTCCAACAACACAAGAGAAGGC10640.17952503479984816No Hit
TCTACCGGGATACCAGCCACGTCCAGGAAGAGCAGACAGAGGTCGAGGAGAC9980.16838908339309064No Hit
TCTACCGGGCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCAT9900.16703927110136246No Hit
TCTACCGGATCACAGACAAGGAAAAGGCAGAGAAGCTGAAGCAGTCCCTACC9900.16703927110136246No Hit
TCTACCGGGATTTCTGGTGGTCTCAGGCAATCTTGGAGAATTTGCATAGGGG9860.1663643649554984No Hit
TCTACCGGGGGTGACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAG9830.1658581853461003No Hit
TCTACCGGGAGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9590.16180874847091575No Hit
TCTACCGGGAGATTAATTTGTTTTATAATGTTTATATGCTATTTAGAATGTT9300.1569156789134011No Hit
TCTACCGGGGAGTTCCCTGGGTCCTGGAGGGTGCAGGGTTTGTGGAACAGTA9020.15219133589235248No Hit
TCTACCGGGATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8990.1516851562829544No Hit
TCTACCGAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8690.14662336018897373No Hit
TCTACCGGGATTTCTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8510.14358628253258532No Hit
TCTACCGGGGATGGTAGTCGCCGTGCCTACCATGGTGACCACGGGTGACGGG8300.14004302526679885No Hit
TCTACCGGGGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTA8250.13919939258446873No Hit
TCTACCGGGCACTCTGGAAGAAATTTGGACCAAAAAACTGTTTGTGACACCA8130.13717467414687645No Hit
TCTACCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTTCTC7910.13346269034462394No Hit
TCTACCGGGGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7600.12823216771417725No Hit
TCTACCGGGATGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7550.12738853503184713No Hit
TCTACCGGGGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGAAGGCA7490.126376175813051No Hit
TCTACCGGGATTTCCGCCACTGGCATATTCAGTCTTTTGTATTTTTGATTAT7420.12519509005778884No Hit
TCTACCGGGGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7350.12401400430252668No Hit
TCTACCGGGAGAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA7250.12232673893786646No Hit
TCTACCGGGGCTCTGGCTGGGCACAGTGGCTACAGCAGTCATTCCTTCAGTT7240.12215801240140042No Hit
TCTACCGGGGTACCAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7210.12165183279200235No Hit
TCTACCGGGGCTACTCAACAGGAATTGATTCTGTTGTCAGGGAATTGGCCGT7130.12030202050027418No Hit
TCTACCGGGTGAGGTTTGGAATGACAAAGACAGGAGAGGAGATGCTGGAGCG7090.11962711435441009No Hit
TCTACCGGGAGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6910.11659003669802168No Hit
TCTACCGGGATTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6900.11642131016155566No Hit
TCTACCGGGAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6880.11608385708862361No Hit
TCTACCGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAAC6830.1152402244062935No Hit
TCTACCGGGAAACATTCTTCCCTCAACATTTCCTGGGCCTTTCAGGAATACC6760.11405913865103134No Hit
TCTACCGGGAGGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTA6720.11338423250516726No Hit
TCTACCGGGATGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTCCGGAG6660.11237187328637112No Hit
TCTACCGAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6570.1108533344581769No Hit
TCTACCGGGACTCTCTTTGTGGAGAAGGAACGAGATAAGGAAGTCAGTGATG6470.10916606909351668No Hit
TCTACCGGGGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGG6360.10731007719239044No Hit
TCTACCGGGAGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6250.10545408529126418No Hit
TCTACCGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6220.10494790568186611No Hit
TCTACCGGGATTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6150.10376681992660394No Hit
TCTACCGGGAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTT6140.10359809339013794No Hit
TCTACCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC6040.1019108280254777No Hit
TCTACCGGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5970.10072974227021556No Hit
TCTACCGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGG5940.10022356266081749No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGAC351.0184522E-746.00000420
CTTCGCG351.0184522E-746.00000427
AGCGTGA351.0184522E-746.00000441
AGGTAAG351.0184522E-746.00000418
GCCCATC351.0184522E-746.00000416
TTGGCCG700.046.00000445
GAAGCCG351.0184522E-746.00000424
ACCGGAC4250.046.0000044
CATATTC700.046.00000424
TCCGCCA700.046.00000413
GTATTAG351.0184522E-746.00000446
ATAGGTA351.0184522E-746.00000435
GTCGAAC351.0184522E-746.00000438
GGTCGAA351.0184522E-746.00000437
CTTCCGT551.8189894E-1246.00000440
TAGACGT351.0184522E-746.00000432
GGGTCGC351.0184522E-746.0000048
CCCTACG351.0184522E-746.00000419
GCGCGCC351.0184522E-746.00000433
CTATATG351.0184522E-746.00000424
Produced by FastQC (version 0.11.5)