SRR1527603_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR1527603_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	323151
Sequences flagged as poor quality	0
Sequence length	50
%GC	46

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GTAATGGGGATGGTGGTGGTGGTAGGGGTACGCTTAGGTGGGCAGTCCTG	1463	0.4527295289199167	No Hit
GTAATGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1415	0.4378757918124963	No Hit
GTAATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1301	0.40259816618237293	No Hit
GTAATGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC	1189	0.36793944626505876	No Hit
GTAATGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	809	0.25034736083131415	No Hit
GTAATGGGGGATTTGCTGTGTAGTCTTGGTGTGGAAATTTGAATTCTGCT	685	0.21197520663714486	No Hit
GTAATGGAGAGAAAAGGCTCCTGCCAGTGTGAAGACAGACGGACTGCTGT	655	0.20269162094500712	No Hit
GTAATGGGGAGAAAGTGGCCACATATTTACAGTTAATGATCAATTCACAT	627	0.19402694096567857	No Hit
GTAATGGGGGAAACACTGTGTCCTTTGTTTAAGGGAAATGATTCATCTTT	598	0.18505280812994546	No Hit
GTAATGGGGGGCAGGGGAGATCTGGAAGGGAATGTCCAAAGAGAAGAAGG	543	0.16803290102769294	No Hit
GTAATGGGGATAGAAGAGGTGGCCTTCATGGTGGAGATTGATCCAGGAAC	527	0.16308165532521948	No Hit
GTAATGGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGTAATGGGGA	526	0.1627722024688149	No Hit
GTAATGGGGCGAAGGATGCCGCCGTGCTGTCTGTGGCCGCCCTCCCATGT	456	0.1411105025204935	No Hit
GTAATGGGGGTACCTTGCTCTCTACGGTCTCTTTGGTTTCTGTCTGTAAG	437	0.1352308982488063	No Hit
GTAATGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGCCTGCA	427	0.1321363696847604	No Hit
GTAATGGGGGTATTGGCCACCAACTGTAGATGTATATACGGTGTCCTTCT	425	0.1315174639719512	No Hit
GTAATGGGGATCTGGATAGTATGGTTTAAAAATATGAAAATATGTTAAGA	409	0.12656621826947773	No Hit
GTAATGGGGAAAATAACAATGTACTAGTCAGAATGGCCGTTCTAAAAGTC	406	0.12563785970026398	No Hit
GTAATGGGGGCACATGGCTTGTGACGAATGAATCTTGCATTTTTTAGTGA	405	0.12532840684385937	No Hit
GTAATGGGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	384	0.11882989685936295	No Hit
GTAATGGGGGTGGACTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTG	379	0.11728263257734	No Hit
GTAATGGGGTGCTGGCTCTTGTACCTTGGGAGATTGTAACAGTGACCAAA	376	0.11635427400812624	No Hit
GTAATGGGGATTGATGTGACGATGTGGGCAGACTCTCTGCATTTAGATGC	375	0.11604482115172164	No Hit
GTAATGGGGAGGCCAGGGTCTGGACTGCTCCGGCCCACAGGGCTGCCCAG	364	0.11264083973127113	No Hit
GTAATGGGGACCCTGTTACACACTCACACAGTTACCTCCCAGGCAGCCAG	361	0.11171248116205737	No Hit
GTAATGGGGCAACTTTCAGGAACCAAGGACTGCCAGAAACTCTCTGCCTC	357	0.11047466973643899	No Hit
GTAATGGGGAGTTCTATGAGATGACTGAGTACAGGTTTGCCTACTGGAGA	351	0.10861795259801145	No Hit
GTAATGGGGGTAACACGGCAATTATTGTGCCTATTTTAGCCCACCGTGGG	351	0.10861795259801145	No Hit
GTAATGGGGAAGGGGCTCCCCCCTCTGCTCTGCACCAGCCCGCCCTGGGA	349	0.10799904688520229	No Hit
GTAATGGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCC	344	0.10645178260317932	No Hit
GTAATGGGGATCCCTCCAGTGTAGCTCTTTTCTCTCCACCATGTAATTTT	344	0.10645178260317932	No Hit
GTAATGGGGAAGGTTTAAAAAAAGGAAAAAAAGTCTAGAGAAAAAAAAAT	343	0.10614232974677472	No Hit
GTAATGGGAGTTGTCTATGATGGGAAGAAAGGTACCAGGACCCCTGAGCT	339	0.10490451832115637	No Hit
GTAATGGGGGTTCTCCCAAAAAGAGACCTCCCATGCTCAGGAAAAGTTGG	337	0.10428561260834718	No Hit
GTAATGGGGTTTCTGGTCGGGGAGATTCCACATGCCTCGGGGCAGCTAAG	336	0.10397615975194258	No Hit
GTAATGGGGCTGGGCCATTCTCTGCGCCTCTGGCTGGTTTTCATGAATTG	335	0.103666706895538	No Hit
GTAATGGGGGAGTAGAATTTCCCTTCTGTCCCTTGTCACAAGTTTAAAAA	330	0.10211944261351504	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCATAAG	20	7.8480726E-4	44.000004	15
TCACGAT	40	8.28004E-9	44.000004	39
TTAGGTA	20	7.8480726E-4	44.000004	28
CGTGGGA	40	8.28004E-9	44.000004	14
CGGGTAA	20	7.8480726E-4	44.000004	14
AGGTAGA	20	7.8480726E-4	44.000004	30
AGGTAAC	20	7.8480726E-4	44.000004	44
TCTGACG	20	7.8480726E-4	44.000004	33
TCGTTCA	20	7.8480726E-4	44.000004	19
TACCCGA	40	8.28004E-9	44.000004	17
ACACGGC	20	7.8480726E-4	44.000004	13
ACGCCGT	20	7.8480726E-4	44.000004	18
CTACTCG	20	7.8480726E-4	44.000004	42
GGTCGTC	20	7.8480726E-4	44.000004	40
AATCACC	20	7.8480726E-4	44.000004	19
GTTTCGA	20	7.8480726E-4	44.000004	14
CACGGAA	40	8.28004E-9	44.000004	28
TTAGCGC	80	0.0	44.000004	20
ACCGCTC	20	7.8480726E-4	44.000004	39
CTATATG	20	7.8480726E-4	44.000004	38

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content