##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527603_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	323151
Sequences flagged as poor quality	0
Sequence length	50
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.996042097966587	31.0	31.0	33.0	28.0	34.0
2	30.22560041590464	31.0	30.0	33.0	26.0	33.0
3	31.424114423288184	31.0	31.0	34.0	30.0	34.0
4	35.471593775046344	37.0	35.0	37.0	33.0	37.0
5	35.58151142964125	37.0	35.0	37.0	35.0	37.0
6	35.991344603606365	37.0	35.0	37.0	35.0	37.0
7	36.470844899133844	37.0	37.0	37.0	35.0	37.0
8	36.54221091687787	37.0	37.0	37.0	35.0	37.0
9	38.15943320614821	39.0	39.0	39.0	37.0	39.0
10	36.20498776113953	38.0	35.0	39.0	32.0	39.0
11	36.411417572589905	37.0	35.0	39.0	33.0	39.0
12	36.774560499580694	39.0	37.0	39.0	33.0	39.0
13	36.91152742835393	39.0	37.0	39.0	33.0	39.0
14	37.73902602807975	40.0	37.0	40.0	33.0	40.0
15	37.82540669841653	40.0	37.0	40.0	34.0	40.0
16	37.95044112504681	40.0	38.0	40.0	34.0	40.0
17	37.86474434552268	40.0	37.0	40.0	34.0	40.0
18	37.74263424838543	40.0	37.0	40.0	34.0	40.0
19	37.67975652249258	39.0	37.0	40.0	34.0	40.0
20	37.53652936243428	39.0	37.0	40.0	33.0	40.0
21	37.48667960179606	39.0	36.0	40.0	33.0	40.0
22	37.34066427150156	39.0	36.0	40.0	33.0	40.0
23	37.1067859916881	38.0	36.0	40.0	33.0	40.0
24	36.907931586162505	38.0	36.0	40.0	33.0	40.0
25	36.60154850209345	38.0	35.0	40.0	32.0	40.0
26	36.10399163239476	38.0	35.0	39.0	31.0	40.0
27	35.83326370644064	38.0	34.0	39.0	31.0	40.0
28	35.66173398813558	37.0	34.0	39.0	31.0	40.0
29	35.35061627536353	37.0	34.0	38.0	30.0	40.0
30	35.02708640852109	36.0	34.0	38.0	30.0	40.0
31	34.82676829098471	36.0	34.0	38.0	30.0	40.0
32	34.74872118607091	36.0	34.0	38.0	30.0	40.0
33	34.32990150115581	36.0	33.0	38.0	29.0	39.0
34	33.945950964100376	35.0	33.0	38.0	27.0	39.0
35	33.66368044660236	35.0	32.0	38.0	27.0	39.0
36	33.32653774860669	35.0	32.0	38.0	26.0	39.0
37	32.940018752843095	35.0	31.0	38.0	24.0	39.0
38	32.4772258170329	34.0	31.0	37.0	24.0	38.0
39	31.713505451012065	34.0	30.0	37.0	22.0	38.0
40	31.37523015556195	34.0	30.0	36.0	21.0	38.0
41	31.00542161404421	33.0	29.0	36.0	20.0	38.0
42	30.702232083453247	33.0	29.0	36.0	19.0	38.0
43	30.286540966916395	33.0	28.0	35.0	18.0	38.0
44	30.047448406472515	32.0	27.0	35.0	18.0	38.0
45	29.64835015209608	32.0	27.0	35.0	18.0	37.0
46	29.500379079749095	32.0	27.0	35.0	18.0	37.0
47	28.954213355366377	31.0	26.0	35.0	17.0	37.0
48	28.47701538909055	31.0	25.0	34.0	15.0	37.0
49	27.935311974897186	30.0	24.0	34.0	15.0	36.0
50	31.016339110818162	33.0	29.0	36.0	20.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	1.0
12	6.0
13	23.0
14	45.0
15	55.0
16	84.0
17	147.0
18	229.0
19	344.0
20	488.0
21	763.0
22	1065.0
23	1432.0
24	2030.0
25	2743.0
26	3857.0
27	5470.0
28	6995.0
29	8457.0
30	9484.0
31	11697.0
32	17904.0
33	24390.0
34	32864.0
35	71631.0
36	98710.0
37	21656.0
38	578.0
39	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	100.0	0.0	0.0	0.0
2	0.0	0.0	100.0	0.0
3	0.0	100.0	0.0	0.0
4	0.0	100.0	0.0	0.0
5	0.0	0.0	100.0	0.0
6	100.0	0.0	0.0	0.0
7	99.46897889840972	0.28871951502548343	0.17143688244814345	0.07086470411665136
8	98.0922231402657	1.4544284251015778	0.21228465949354944	0.2410637751391764
9	90.04799613802835	4.833963069896117	3.3383774148927277	1.7796633771828032
10	35.14022856187974	49.96920944078774	7.4355332336895135	7.455028763643003
11	27.628879378371103	31.099083710092184	28.138238780013058	13.133798131523653
12	27.853851604977244	26.029008110759367	24.18652580372643	21.93061448053696
13	24.58541053563195	26.495353565361086	27.136849336687803	21.782386562319164
14	24.145678026681026	27.74182967095878	26.56993170375459	21.542560598605608
15	24.80728823367404	29.08733069060594	25.627647756002613	20.47773331971741
16	26.557244136642005	31.086705595836	23.605373339398607	18.750676928123386
17	26.61511182078966	29.869008605883934	24.015707826991097	19.500171746335305
18	25.15232816856516	30.08345943537232	22.243471318362005	22.520741077700517
19	22.65751924023135	31.480948534895454	23.78918833610294	22.072343888770266
20	22.25925341403864	31.067210065882513	25.096007748699524	21.577528771379324
21	22.84504767121253	30.175985839437292	27.188218510850966	19.790747978499216
22	23.194419946093312	29.0576232163911	26.15402706474682	21.593929772768767
23	21.359983413326898	31.742745651413735	26.31587090864642	20.581400026612943
24	21.29190378491789	33.13497405237799	25.804964242722445	19.76815791998168
25	22.86392429545321	31.367998242307777	24.615736915559598	21.152340546679415
26	23.68026093064852	33.522099575740135	24.459772675931685	18.33786681767966
27	22.91250839390873	30.237566957861805	23.45497925118598	23.394945397043486
28	22.876611862565795	32.31956577575189	24.308140776293435	20.495681585388876
29	23.746793294775507	30.420453595996914	24.918072356266883	20.91468075296069
30	23.35873941284415	32.12863336335026	23.63755643646469	20.875070787340903
31	24.009518769863007	30.25953811066653	22.89084669396041	22.840096425510055
32	23.263427933071537	31.81732378980724	24.819666347930223	20.099581929190997
33	25.094460484417503	31.538506766186707	23.739056973365393	19.6279757760304
34	22.62935903029853	32.420447406939786	24.9932694003732	19.956924162388482
35	21.5490591085901	33.94728780044004	24.88403254206238	19.619620548907477
36	24.330111929098162	31.091037935825668	24.795529025130666	19.783321109945508
37	22.88620490111434	32.51142654672274	25.123858505775935	19.478510046386983
38	24.999767910357697	31.146739449978494	23.299943370127277	20.553549269536532
39	24.006733694155365	29.928423554313614	24.021587431262784	22.043255320268234
40	24.639564785502753	30.338758041906104	22.904153166785807	22.117524005805336
41	23.912969478664774	31.450003249254994	23.28385182159424	21.353175450485995
42	24.003329712734914	30.962305547561357	23.999306825601654	21.035057914102076
43	22.76954117424981	31.38068580942036	24.632447369805448	21.21732564652438
44	23.052999990716412	30.7967482693849	24.28152783064264	21.868723909256044
45	22.26482356545392	30.726193018124654	25.214837645558884	21.79414577086254
46	22.631215747436954	32.60550021506973	23.13067265767397	21.63261137981934
47	22.004883166074066	32.076335830617886	23.714919650565836	22.203861352742216
48	23.32284288150122	30.617265612670234	24.252129809284202	21.80776169654434
49	22.26946535829999	31.90489894816974	24.261103942119938	21.564531751410332
50	22.218405636993232	31.767811332782507	25.03659280026984	20.977190229954417
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.5
4	1.0
5	3.5
6	6.0
7	143.0
8	280.0
9	1340.0
10	2400.0
11	2858.0
12	3316.0
13	3255.5
14	3195.0
15	3024.5
16	2854.0
17	2565.0
18	2276.0
19	2327.0
20	2378.0
21	2575.0
22	2772.0
23	3168.5
24	3565.0
25	4504.0
26	5443.0
27	5826.0
28	6209.0
29	6600.5
30	6992.0
31	8784.0
32	10576.0
33	9896.5
34	9217.0
35	10210.5
36	11204.0
37	11831.0
38	12458.0
39	12394.0
40	12330.0
41	13490.5
42	14651.0
43	16642.5
44	18634.0
45	18934.5
46	19235.0
47	19868.0
48	20501.0
49	20075.0
50	19649.0
51	20560.0
52	21471.0
53	21499.0
54	21527.0
55	21867.5
56	22208.0
57	21237.5
58	20267.0
59	18020.0
60	15773.0
61	13763.5
62	11754.0
63	9842.5
64	7931.0
65	6200.0
66	4469.0
67	3752.5
68	3036.0
69	3216.0
70	3396.0
71	1932.0
72	468.0
73	536.0
74	604.0
75	313.0
76	22.0
77	17.5
78	13.0
79	31.5
80	50.0
81	33.5
82	17.0
83	9.5
84	2.0
85	1.5
86	1.0
87	0.5
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	323151.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.966681210950917
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.51645144816517	13.321636015361241
2	7.700445028087838	2.6130199194803665
3	2.495075508864084	1.2699945226844418
4	1.1198657620194061	0.7600162153296756
5	0.6493032756985482	0.550826084400172
6	0.47421025753264756	0.48274645599116206
7	0.34289049390822207	0.40723995902844184
8	0.31370832421390527	0.4258071304127173
9	0.27358284088421975	0.41776135614619797
>10	5.334865397242285	23.667882816392336
>50	1.6469687021230028	19.30923933393367
>100	1.1107463339899322	33.57470656132892
>500	0.014591084847158386	1.5379806963308176
>1k	0.007295542423579193	1.6611429331798446
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTAATGGGGATGGTGGTGGTGGTAGGGGTACGCTTAGGTGGGCAGTCCTG	1463	0.4527295289199167	No Hit
GTAATGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1415	0.4378757918124963	No Hit
GTAATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1301	0.40259816618237293	No Hit
GTAATGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC	1189	0.36793944626505876	No Hit
GTAATGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	809	0.25034736083131415	No Hit
GTAATGGGGGATTTGCTGTGTAGTCTTGGTGTGGAAATTTGAATTCTGCT	685	0.21197520663714486	No Hit
GTAATGGAGAGAAAAGGCTCCTGCCAGTGTGAAGACAGACGGACTGCTGT	655	0.20269162094500712	No Hit
GTAATGGGGAGAAAGTGGCCACATATTTACAGTTAATGATCAATTCACAT	627	0.19402694096567857	No Hit
GTAATGGGGGAAACACTGTGTCCTTTGTTTAAGGGAAATGATTCATCTTT	598	0.18505280812994546	No Hit
GTAATGGGGGGCAGGGGAGATCTGGAAGGGAATGTCCAAAGAGAAGAAGG	543	0.16803290102769294	No Hit
GTAATGGGGATAGAAGAGGTGGCCTTCATGGTGGAGATTGATCCAGGAAC	527	0.16308165532521948	No Hit
GTAATGGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGTAATGGGGA	526	0.1627722024688149	No Hit
GTAATGGGGCGAAGGATGCCGCCGTGCTGTCTGTGGCCGCCCTCCCATGT	456	0.1411105025204935	No Hit
GTAATGGGGGTACCTTGCTCTCTACGGTCTCTTTGGTTTCTGTCTGTAAG	437	0.1352308982488063	No Hit
GTAATGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGCCTGCA	427	0.1321363696847604	No Hit
GTAATGGGGGTATTGGCCACCAACTGTAGATGTATATACGGTGTCCTTCT	425	0.1315174639719512	No Hit
GTAATGGGGATCTGGATAGTATGGTTTAAAAATATGAAAATATGTTAAGA	409	0.12656621826947773	No Hit
GTAATGGGGAAAATAACAATGTACTAGTCAGAATGGCCGTTCTAAAAGTC	406	0.12563785970026398	No Hit
GTAATGGGGGCACATGGCTTGTGACGAATGAATCTTGCATTTTTTAGTGA	405	0.12532840684385937	No Hit
GTAATGGGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	384	0.11882989685936295	No Hit
GTAATGGGGGTGGACTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTG	379	0.11728263257734	No Hit
GTAATGGGGTGCTGGCTCTTGTACCTTGGGAGATTGTAACAGTGACCAAA	376	0.11635427400812624	No Hit
GTAATGGGGATTGATGTGACGATGTGGGCAGACTCTCTGCATTTAGATGC	375	0.11604482115172164	No Hit
GTAATGGGGAGGCCAGGGTCTGGACTGCTCCGGCCCACAGGGCTGCCCAG	364	0.11264083973127113	No Hit
GTAATGGGGACCCTGTTACACACTCACACAGTTACCTCCCAGGCAGCCAG	361	0.11171248116205737	No Hit
GTAATGGGGCAACTTTCAGGAACCAAGGACTGCCAGAAACTCTCTGCCTC	357	0.11047466973643899	No Hit
GTAATGGGGAGTTCTATGAGATGACTGAGTACAGGTTTGCCTACTGGAGA	351	0.10861795259801145	No Hit
GTAATGGGGGTAACACGGCAATTATTGTGCCTATTTTAGCCCACCGTGGG	351	0.10861795259801145	No Hit
GTAATGGGGAAGGGGCTCCCCCCTCTGCTCTGCACCAGCCCGCCCTGGGA	349	0.10799904688520229	No Hit
GTAATGGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCC	344	0.10645178260317932	No Hit
GTAATGGGGATCCCTCCAGTGTAGCTCTTTTCTCTCCACCATGTAATTTT	344	0.10645178260317932	No Hit
GTAATGGGGAAGGTTTAAAAAAAGGAAAAAAAGTCTAGAGAAAAAAAAAT	343	0.10614232974677472	No Hit
GTAATGGGAGTTGTCTATGATGGGAAGAAAGGTACCAGGACCCCTGAGCT	339	0.10490451832115637	No Hit
GTAATGGGGGTTCTCCCAAAAAGAGACCTCCCATGCTCAGGAAAAGTTGG	337	0.10428561260834718	No Hit
GTAATGGGGTTTCTGGTCGGGGAGATTCCACATGCCTCGGGGCAGCTAAG	336	0.10397615975194258	No Hit
GTAATGGGGCTGGGCCATTCTCTGCGCCTCTGGCTGGTTTTCATGAATTG	335	0.103666706895538	No Hit
GTAATGGGGGAGTAGAATTTCCCTTCTGTCCCTTGTCACAAGTTTAAAAA	330	0.10211944261351504	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.007117415697305594	0.0	0.0	0.0	0.0
8	0.00897413283573314	0.0	0.0	0.0	0.0
9	0.022899511373939738	0.0	0.0	0.0	0.0
10	0.07241196839867431	0.0	0.0	0.0	0.0
11	0.12532840684385937	0.0	0.0	0.0	0.0
12	0.15379806963308174	0.0	0.0	0.0	0.0
13	0.1723652410173572	0.0	0.0	0.0	0.0
14	0.20269162094500714	0.0	0.0	0.0	0.0
15	0.2311612837342295	0.0	0.0	0.0	0.0
16	0.2590120408106427	0.0	0.0	0.0	0.0
17	0.2788170236205365	0.0	0.0	0.0	0.0
18	0.3066677806969497	0.0	0.0	0.0	0.0
19	0.34101704775785935	0.0	0.0	0.0	0.0
20	0.3747474091059598	0.0	0.0	0.0	0.0
21	0.4171424504333887	0.0	0.0	0.0	0.0
22	0.4564429631967718	0.0	0.0	0.0	0.0
23	0.4945056645345365	0.0	0.0	0.0	0.0
24	0.5446370272720802	0.0	0.0	0.0	0.0
25	0.5857942571738909	0.0	0.0	0.0	0.0
26	0.6315932799217703	0.0	0.0	0.0	0.0
27	0.6758450383876269	0.0	0.0	0.0	0.0
28	0.7464002896478736	0.0	0.0	0.0	0.0
29	0.7792022924267602	0.0	0.0	0.0	0.0
30	0.8126232009184561	0.0	0.0	0.0	0.0
31	0.8395455994256555	0.0	0.0	0.0	0.0
32	0.8658490922200457	0.0	0.0	0.0	0.0
33	0.9014361707065737	0.0	0.0	0.0	0.0
34	0.9295963806393915	0.0	0.0	0.0	0.0
35	0.972300874823225	0.0	0.0	0.0	0.0
36	0.9952003861971648	0.0	0.0	0.0	0.0
37	1.0137675575814402	0.0	0.0	0.0	0.0
38	1.0360481632425709	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCATAAG	20	7.8480726E-4	44.000004	15
TCACGAT	40	8.28004E-9	44.000004	39
TTAGGTA	20	7.8480726E-4	44.000004	28
CGTGGGA	40	8.28004E-9	44.000004	14
CGGGTAA	20	7.8480726E-4	44.000004	14
AGGTAGA	20	7.8480726E-4	44.000004	30
AGGTAAC	20	7.8480726E-4	44.000004	44
TCTGACG	20	7.8480726E-4	44.000004	33
TCGTTCA	20	7.8480726E-4	44.000004	19
TACCCGA	40	8.28004E-9	44.000004	17
ACACGGC	20	7.8480726E-4	44.000004	13
ACGCCGT	20	7.8480726E-4	44.000004	18
CTACTCG	20	7.8480726E-4	44.000004	42
GGTCGTC	20	7.8480726E-4	44.000004	40
AATCACC	20	7.8480726E-4	44.000004	19
GTTTCGA	20	7.8480726E-4	44.000004	14
CACGGAA	40	8.28004E-9	44.000004	28
TTAGCGC	80	0.0	44.000004	20
ACCGCTC	20	7.8480726E-4	44.000004	39
CTATATG	20	7.8480726E-4	44.000004	38
>>END_MODULE