FastQCFastQC Report
Fri 17 Jun 2016
SRR1527602_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527602_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences292469
Sequences flagged as poor quality0
Sequence length52
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13420.4588520492770174No Hit
GTAATGGGGATGGTGGTGGTGGTAGGGGTACGCTTAGGTGGGCAGTCCTGTG12920.44175622031736694No Hit
GTAATGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA12670.4332083058375418No Hit
GTAATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12050.4120094779275752No Hit
GTAATGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7750.2649853488745816No Hit
GTAATGGGGGATTTGCTGTGTAGTCTTGGTGTGGAAATTTGAATTCTGCTTT6590.2253230256881926No Hit
GTAATGGAGAGAAAAGGCTCCTGCCAGTGTGAAGACAGACGGACTGCTGTGA6250.21369786199563032No Hit
GTAATGGGGGAAACACTGTGTCCTTTGTTTAAGGGAAATGATTCATCTTTCT5940.20309844804064703No Hit
GTAATGGGGAGAAAGTGGCCACATATTTACAGTTAATGATCAATTCACATCT5400.1846349527642246No Hit
GTAATGGGGGGCAGGGGAGATCTGGAAGGGAATGTCCAAAGAGAAGAAGGAG5360.18326728644745255No Hit
GTAATGGGGCGAAGGATGCCGCCGTGCTGTCTGTGGCCGCCCTCCCATGTCC4740.16206845853748603No Hit
GTAATGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGCCTGCACT4520.15454629379523982No Hit
GTAATGGGGGTACCTTGCTCTCTACGGTCTCTTTGGTTTCTGTCTGTAAGTT4430.15146904458250277No Hit
GTAATGGGGATAGAAGAGGTGGCCTTCATGGTGGAGATTGATCCAGGAACTA4420.15112712800330974No Hit
GTAATGGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGTAATGGGGAAG4420.15112712800330974No Hit
GTAATGGGGGCACATGGCTTGTGACGAATGAATCTTGCATTTTTTAGTGATC4180.14292113010267754No Hit
GTAATGGGGATCTGGATAGTATGGTTTAAAAATATGAAAATATGTTAAGAAC3780.1292444669349572No Hit
GTAATGGGGCAACTTTCAGGAACCAAGGACTGCCAGAAACTCTCTGCCTCTA3750.12821871719737818No Hit
GTAATGGGGGTATTGGCCACCAACTGTAGATGTATATACGGTGTCCTTCTGA3490.11932888613835997No Hit
GTAATGGGGTTTCTGGTCGGGGAGATTCCACATGCCTCGGGGCAGCTAAGCC3460.11830313640078094No Hit
GTAATGGGGAAGGTTTAAAAAAAGGAAAAAAAGTCTAGAGAAAAAAAAATAC3440.11761930324239492No Hit
GTAATGGGGAATTTCATCACTGGAGTGAGAGCAATAAACGAGTTCTGCCTTA3430.11727738666320192No Hit
GTAATGGGGGTAACACGGCAATTATTGTGCCTATTTTAGCCCACCGTGGGCT3410.11659355350481589No Hit
GTAATGGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCAT3390.11590972034642988No Hit
GTAATGGGGGTGGACTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCT3370.11522588718804387No Hit
GTAATGGGGAGGCCAGGGTCTGGACTGCTCCGGCCCACAGGGCTGCCCAGCC3320.11351630429207882No Hit
GTAATGGGGTGCTGGCTCTTGTACCTTGGGAGATTGTAACAGTGACCAAATT3310.11317438771288582No Hit
GTAATGGGGGGCCTGGCTGGACCTTGTTCCCTGGGGGAGGGGGCGCTTGCCC3300.1128324711336928No Hit
GTAATGGGGATTGATGTGACGATGTGGGCAGACTCTCTGCATTTAGATGCCC3290.11249055455449979No Hit
GTAATGGGGAAAATAACAATGTACTAGTCAGAATGGCCGTTCTAAAAGTCTA3270.11180672139611378No Hit
GTAATGGGGGAGTAGAATTTCCCTTCTGTCCCTTGTCACAAGTTTAAAAACC3250.11112288823772774No Hit
GTAATGGGGAGTTCTATGAGATGACTGAGTACAGGTTTGCCTACTGGAGACC3250.11112288823772774No Hit
GTAATGGGGATCCCTCCAGTGTAGCTCTTTTCTCTCCACCATGTAATTTTCC3230.11043905507934174No Hit
GTAATGGGGGAGTGCAGTGCTGTAATGGGGAAAAAAAAAAAAAAAAAAAAAA3150.10770372244579768No Hit
GTAATGGGGGAAGGTCTGCCTGAAAACCAGCCTGCTGGTGGGGCAGTCCTGA3120.10667797270821865No Hit
GTAATGGGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3080.1053103063914466No Hit
GTAATGGGGAAGGGGCTCCCCCCTCTGCTCTGCACCAGCCCGCCCTGGGACC3080.1053103063914466No Hit
GTAATGGGGAGATGGCCCATTATTGAATCGAGTCGAAAACAAAGCACAAACC3070.1049683898122536No Hit
GTAATGGGGGACTCTGTGCTTGTCTGTTTAGTTCTGTGTGTAAATGAAATGT3040.10394264007467459No Hit
GTAATGGGGGAGAGGAAAATTCAAGATGGTCAAAGATGTCCTGCAGGATGTA2990.10223305717870955No Hit
GTAATGGGGATGTGTGATCTGTGATGTTGAAAGCAGTTTAGCACAAAATAGG2980.10189114059951654No Hit
GTAATGGGGACCCTGTTACACACTCACACAGTTACCTCCCAGGCAGCCAGAT2980.10189114059951654No Hit
GTAATGGGGACCCTTTGCTGCCCTGGGGCCATGGCCTCACCCCTCTGGAGGG2970.10154922402032351No Hit
GTAATGGGGAACCTCAAACTCGACATTAATACTGTATAGCTGAGGCTGGCCT2960.1012073074411305No Hit
GTAATGGGTACCGCTGAAGACCTGCGCTAGGGCTGCTCAGCCCTCAGCCCTC2950.1008653908619375No Hit
GTAATGGGGGGATCTGCCATGGAGGAATCTGGTACCTCCAGATGGGTGCACA2940.1005234742827445No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTAAGC351.0160693E-746.00000413
CCTTAAG351.0160693E-746.00000412
CGGCCTA351.0160693E-746.00000420
TAGACGG351.0160693E-746.00000436
GTCTACT351.0160693E-746.00000422
GTAGGGC351.0160693E-746.0000049
TGCGGCA351.0160693E-746.00000414
ACGGGTT351.0160693E-746.00000414
AAACGTA351.0160693E-746.00000437
TCTACGG351.0160693E-746.00000421
ATCCCGG351.0160693E-746.00000427
ACGTGTG351.0160693E-746.00000418
GAAACGT351.0160693E-746.00000436
ACGTGGG351.0160693E-746.00000434
TCCACGC351.0160693E-746.00000412
CGCAGTA351.0160693E-746.00000416
ACTAACT351.0160693E-746.00000423
GCGGCCT351.0160693E-746.00000419
GATTATA351.0160693E-746.00000431
AGTCGAG351.0160693E-746.00000436
Produced by FastQC (version 0.11.5)