FastQCFastQC Report
Fri 17 Jun 2016
SRR1527601_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527601_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences312423
Sequences flagged as poor quality0
Sequence length52
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATGGGGATGGTGGTGGTGGTAGGGGTACGCTTAGGTGGGCAGTCCTGTG14480.4634742000428906No Hit
GTAATGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13310.4260249725532371No Hit
GTAATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12750.4081005559769927No Hit
GTAATGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA11750.3760926692336992No Hit
GTAATGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7650.24486033358619563No Hit
GTAATGGAGAGAAAAGGCTCCTGCCAGTGTGAAGACAGACGGACTGCTGTGA7550.2416595449118663No Hit
GTAATGGGGGAAACACTGTGTCCTTTGTTTAAGGGAAATGATTCATCTTTCT6040.19332763592949304No Hit
GTAATGGGGAGAAAGTGGCCACATATTTACAGTTAATGATCAATTCACATCT5890.188526452917999No Hit
GTAATGGGGGGCAGGGGAGATCTGGAAGGGAATGTCCAAAGAGAAGAAGGAG5660.18116463896704146No Hit
GTAATGGGGATAGAAGAGGTGGCCTTCATGGTGGAGATTGATCCAGGAACTA5070.16227998578849828No Hit
GTAATGGGGCGAAGGATGCCGCCGTGCTGTCTGTGGCCGCCCTCCCATGTCC4810.15395793523524198No Hit
GTAATGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGCCTGCACT4730.15139730429577847No Hit
GTAATGGGGGTACCTTGCTCTCTACGGTCTCTTTGGTTTCTGTCTGTAAGTT4410.14115478053792455No Hit
GTAATGGGGGAGTAGAATTTCCCTTCTGTCCCTTGTCACAAGTTTAAAAACC4230.1353933609241317No Hit
GTAATGGGGGTATTGGCCACCAACTGTAGATGTATATACGGTGTCCTTCTGA4090.1309122567800706No Hit
GTAATGGGGAGGCCAGGGTCTGGACTGCTCCGGCCCACAGGGCTGCCCAGCC4050.12963194131033887No Hit
GTAATGGGGATTGATGTGACGATGTGGGCAGACTCTCTGCATTTAGATGCCC4020.12867170470804007No Hit
GTAATGGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGTAATGGGGAAG3990.12771146810574124No Hit
GTAATGGGGATCTGGATAGTATGGTTTAAAAATATGAAAATATGTTAAGAAC3950.12643115263600951No Hit
GTAATGGGGAAGGGGCTCCCCCCTCTGCTCTGCACCAGCCCGCCCTGGGACC3860.1235504428291131No Hit
GTAATGGGGGCACATGGCTTGTGACGAATGAATCTTGCATTTTTTAGTGATC3850.12323036396168015No Hit
GTAATGGGGCAACTTTCAGGAACCAAGGACTGCCAGAAACTCTCTGCCTCTA3840.12291028509424723No Hit
GTAATGGGGTTTCTGGTCGGGGAGATTCCACATGCCTCGGGGCAGCTAAGCC3830.1225902062268143No Hit
GTAATGGGGTGCTGGCTCTTGTACCTTGGGAGATTGTAACAGTGACCAAATT3760.12034965415478374No Hit
GTAATGGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCAT3690.1181091020827532No Hit
GTAATGGGGGTAACACGGCAATTATTGTGCCTATTTTAGCCCACCGTGGGCT3610.1155484711432897No Hit
GTAATGGGGAAAATAACAATGTACTAGTCAGAATGGCCGTTCTAAAAGTCTA3590.11490831340842382No Hit
GTAATGGGGACCCTGTTACACACTCACACAGTTACCTCCCAGGCAGCCAGAT3540.11330791907125914No Hit
GTAATGGGGATAGCTTTCTTGTCCTTGTAAGAGGTGGATAAAGATACCCAGG3500.11202760360152743No Hit
GTAATGGGGATCCCTCCAGTGTAGCTCTTTTCTCTCCACCATGTAATTTTCC3490.11170752473409447No Hit
GTAATGGGTACCGCTGAAGACCTGCGCTAGGGCTGCTCAGCCCTCAGCCCTC3490.11170752473409447No Hit
GTAATGGGGAAGGTTTAAAAAAAGGAAAAAAAGTCTAGAGAAAAAAAAATAC3470.1110673669992286No Hit
GTAATGGGAGTTGTCTATGATGGGAAGAAAGGTACCAGGACCCCTGAGCTCT3470.1110673669992286No Hit
GTAATGGGGAGATGGCCCATTATTGAATCGAGTCGAAAACAAAGCACAAACC3430.10978705152949687No Hit
GTAATGGGGGAGAGGAAAATTCAAGATGGTCAAAGATGTCCTGCAGGATGTA3420.10946697266206393No Hit
GTAATGGGGGGATCTGCCATGGAGGAATCTGGTACCTCCAGATGGGTGCACA3420.10946697266206393No Hit
GTAATGGGGGTTCTCCCAAAAAGAGACCTCCCATGCTCAGGAAAAGTTGGGC3410.109146893794631No Hit
GTAATGGGGGGGCTTCCCAGGTGGAGGAGCTCCCCCATCTGGTGGTGCTTCT3400.10882681492719808No Hit
GTAATGGGGGTGGACTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCT3330.10658626285516752No Hit
GTAATGGGGGACTCTGTGCTTGTCTGTTTAGTTCTGTGTGTAAATGAAATGT3300.1056260262528687No Hit
GTAATGGGGCTGGGCCATTCTCTGCGCCTCTGGCTGGTTTTCATGAATTGAG3260.10434571078313697No Hit
GTAATGGGGAGGTGTGACCCATAGCTTTCTTGTCCTTGTAAGAGGTGGATAA3260.10434571078313697No Hit
GTAATGGGGGGGCAGACTGTCCATCCCAAGCCAGTTCAATTAGATGTAAATT3240.1037055530482711No Hit
GTAATGGGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3230.10338547418083815No Hit
GTAATGGGGAGTTCTATGAGATGACTGAGTACAGGTTTGCCTACTGGAGACC3220.10306539531340522No Hit
GTAATGGGGACTGGTTTGTCACTGTCACTGCGCCCAGTCACTCCCCACGTAT3190.10210515871110643No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATGCG551.8189894E-1246.00000434
ACGTTGA551.8189894E-1246.00000415
TACGGTC551.8189894E-1246.00000423
CCCACGT551.8189894E-1246.00000444
TCTACGG551.8189894E-1246.00000421
CTACGGT551.8189894E-1246.00000422
CCACGTA551.8189894E-1246.00000445
TCGCCAG253.4114048E-546.036
CGGGTTC253.4114048E-546.015
CTTCGGC206.3043647E-446.038
GTTTGCG253.4114048E-546.023
AGCGTTC405.5879354E-946.015
AGCGTAC253.4114048E-546.011
AATCCTC253.4114048E-546.027
TAATACA253.4114048E-546.015
ACTATCA253.4114048E-546.022
TCGTTGC206.3043647E-446.021
GATACCG206.3043647E-446.09
CTCACGG253.4114048E-546.011
CCTTAAG405.5879354E-946.012
Produced by FastQC (version 0.11.5)