##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1527601_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 312423 Sequences flagged as poor quality 0 Sequence length 52 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.46405994437029 31.0 31.0 33.0 28.0 33.0 2 30.090719953396516 31.0 30.0 31.0 26.0 33.0 3 31.21469290033064 31.0 31.0 33.0 30.0 34.0 4 35.502571833699825 37.0 35.0 37.0 33.0 37.0 5 35.57351091308899 37.0 35.0 37.0 35.0 37.0 6 35.88485802901835 37.0 35.0 37.0 35.0 37.0 7 36.41437090099 37.0 37.0 37.0 35.0 37.0 8 36.4849066810062 37.0 37.0 37.0 35.0 37.0 9 38.15585600291912 39.0 39.0 39.0 37.0 39.0 10 35.897814821572034 37.0 35.0 39.0 31.0 39.0 11 36.267758775762346 37.0 35.0 39.0 32.0 39.0 12 36.74200042890568 39.0 37.0 39.0 33.0 39.0 13 36.8148407767674 39.0 37.0 39.0 33.0 39.0 14 37.58414713385378 40.0 37.0 40.0 33.0 40.0 15 37.762949590779165 40.0 37.0 40.0 33.0 40.0 16 37.82559222592447 40.0 37.0 40.0 33.0 40.0 17 37.850411141305216 40.0 37.0 40.0 34.0 40.0 18 37.79777417155587 40.0 37.0 40.0 33.0 40.0 19 37.691005463746265 40.0 37.0 40.0 33.0 40.0 20 37.57725263504928 40.0 37.0 40.0 33.0 40.0 21 37.64536861882768 40.0 37.0 40.0 33.0 40.0 22 37.68975715616328 40.0 37.0 40.0 34.0 40.0 23 37.6036079289937 39.0 37.0 40.0 33.0 40.0 24 37.49758500494522 39.0 36.0 40.0 33.0 40.0 25 37.41943454867279 39.0 36.0 40.0 33.0 40.0 26 37.23479385320543 39.0 35.0 40.0 33.0 40.0 27 37.205234569798 39.0 35.0 40.0 33.0 40.0 28 37.00882137358645 39.0 35.0 40.0 33.0 40.0 29 36.958850660802824 39.0 35.0 40.0 33.0 40.0 30 36.87986159789772 39.0 35.0 40.0 33.0 40.0 31 36.77157251546781 39.0 35.0 40.0 32.0 40.0 32 36.65748360396002 39.0 35.0 40.0 32.0 40.0 33 36.508518899056725 38.0 35.0 40.0 31.0 40.0 34 36.36300784513304 38.0 35.0 40.0 31.0 40.0 35 36.22098565086437 38.0 35.0 40.0 31.0 40.0 36 36.08556988441952 38.0 35.0 40.0 31.0 40.0 37 35.917688518450944 38.0 35.0 40.0 30.0 40.0 38 35.703981461032 38.0 35.0 40.0 30.0 40.0 39 35.49085054557443 38.0 35.0 40.0 29.0 40.0 40 35.716813422827386 39.0 35.0 40.0 30.0 40.0 41 35.71399032721663 39.0 35.0 40.0 29.0 40.0 42 35.71947327821575 39.0 35.0 40.0 30.0 40.0 43 35.69740384030625 39.0 35.0 40.0 29.0 40.0 44 35.62012079776457 39.0 35.0 40.0 29.0 40.0 45 35.59789452121002 39.0 35.0 40.0 29.0 40.0 46 35.35191711237649 38.0 35.0 40.0 28.0 40.0 47 35.34747121690785 38.0 35.0 40.0 28.0 40.0 48 35.31847207151842 38.0 35.0 40.0 28.0 40.0 49 35.16181587143072 38.0 35.0 40.0 27.0 40.0 50 34.95083268517363 38.0 34.0 40.0 27.0 40.0 51 34.81132631080298 38.0 34.0 40.0 26.0 40.0 52 34.436497953095646 37.0 34.0 40.0 24.0 40.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 1.0 9 0.0 10 0.0 11 3.0 12 2.0 13 9.0 14 12.0 15 25.0 16 35.0 17 60.0 18 92.0 19 184.0 20 303.0 21 459.0 22 812.0 23 1224.0 24 1698.0 25 2342.0 26 3447.0 27 4562.0 28 5055.0 29 4897.0 30 5224.0 31 6422.0 32 8453.0 33 12562.0 34 21954.0 35 19927.0 36 32549.0 37 55137.0 38 112046.0 39 12927.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 100.0 0.0 0.0 0.0 2 0.0 0.0 100.0 0.0 3 0.0 100.0 0.0 0.0 4 0.0 100.0 0.0 0.0 5 0.0 0.0 100.0 0.0 6 100.0 0.0 0.0 0.0 7 99.52116201432032 0.277828456931788 0.14467564807968683 0.05633388066819665 8 98.17651069223456 1.4473966385317343 0.17572329822068158 0.2003693710130176 9 90.18254097809701 4.782938516050355 3.3144166722680466 1.7201038335845953 10 35.1552222467616 49.7444170243548 7.51929275373452 7.581067975149076 11 27.61416413004164 30.750937030884412 28.1307714220784 13.504127416995548 12 27.593359003658502 26.112994241781173 24.128505263696976 22.16514149086335 13 24.56381252340577 26.426671531865452 27.262077375865413 21.747438568863366 14 24.30486871965252 27.32865378029146 26.72178424763862 21.644693252417397 15 24.43674121303489 28.961696161934302 25.889579192312983 20.71198343271782 16 26.708020856339004 30.867125659762568 23.628221993899295 18.796631489999136 17 26.573267653149735 29.486305425656884 23.94477999379047 19.995646927402912 18 24.921980776063222 29.808624845161845 22.530351478604327 22.739042900170602 19 22.9294898262932 30.932421748718887 23.709522026227262 22.428566398760655 20 22.200350166280973 30.67827912797713 25.506444787995765 21.614925917746135 21 23.249568693726133 29.652106278987144 27.064908793526726 20.033416233759997 22 23.202837179080927 28.784372469376457 26.121636371201863 21.891153980340754 23 21.34125848609097 31.176962003437648 26.563345208259314 20.918434302212066 24 21.589959766086363 32.523853877595435 25.83452562711452 20.051660729203675 25 22.94133274438822 31.039328090441487 24.74305668916821 21.276282476002088 26 23.436174673439535 33.499134186663596 24.513240062351365 18.551451077545508 27 23.095610758490892 30.11045921715111 23.245087589582074 23.548842434775928 28 22.89460122974301 31.79439413871578 24.458826654887762 20.852177976653447 29 23.661510196112324 30.15366986425455 25.041050114748277 21.14376982488485 30 23.326707700777472 31.64619762309433 23.849076412428023 21.178018263700178 31 23.76713622236519 29.979546960371035 22.854911450181326 23.39840536708245 32 23.495069185047196 31.633394468397015 24.575975520368218 20.29556082618757 33 24.972233158250194 31.237456909382473 24.00911584614449 19.781194086222843 34 22.75856771108401 32.184570278116524 25.153397797217238 19.903464213582225 35 21.881551614317768 33.294283711506516 24.980235129936016 19.8439295442397 36 24.508758958207302 30.409092800466038 25.005521360463216 20.076626880863444 37 23.336950224535325 32.022930450062894 25.042970587952873 19.597148737448908 38 25.094503285609576 30.748056321077517 23.43425420023494 20.723186193077975 39 24.138427708587397 29.95842175512046 24.012956792553688 21.890193743738457 40 24.714249591099247 30.13350489560628 22.678868073093213 22.473377440201265 41 24.021598921974373 31.083178895279794 23.39328410520352 21.501938077542306 42 24.281182883462485 30.318510480982514 23.995672533712305 21.404634101842692 43 23.0354359314135 30.718929144141114 24.914298883244832 21.33133604120055 44 23.399045524817318 30.26921833539784 24.30358840418279 22.02814773560205 45 22.650701132759114 30.057006046289807 25.211652151090032 22.080640669861054 46 23.070324527963688 31.856169360130338 23.383361660313103 21.69014445159287 47 22.378634095441114 31.4291841509748 23.770016932172087 22.422164821411997 48 23.499550289191255 29.95714143965073 24.430659714553666 22.11264855660435 49 22.44200971119284 31.419261706084377 24.63294955877128 21.5057790239515 50 22.241000182444953 31.373490428041467 25.131952513099225 21.253556876414347 51 20.952362662159956 31.339562068093578 24.579816466777412 23.12825880296905 52 21.69942673874843 30.4894325961917 25.434106963955923 22.37703370110395 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 1.0 4 2.0 5 3.0 6 4.0 7 137.5 8 271.0 9 1246.5 10 2222.0 11 2617.5 12 3013.0 13 2911.0 14 2623.0 15 2437.0 16 2136.5 17 1836.0 18 1781.5 19 1727.0 20 1901.0 21 2075.0 22 2364.0 23 2653.0 24 2840.5 25 3028.0 26 3968.5 27 4909.0 28 6101.0 29 7293.0 30 7612.5 31 7932.0 32 8359.5 33 8787.0 34 9288.0 35 9789.0 36 10379.5 37 10970.0 38 11084.5 39 11996.0 40 12793.0 41 13807.5 42 14822.0 43 15947.0 44 17072.0 45 17960.0 46 18848.0 47 18116.0 48 17384.0 49 18767.0 50 20150.0 51 19431.0 52 18712.0 53 19490.0 54 20268.0 55 20645.5 56 21023.0 57 19732.5 58 18442.0 59 17248.5 60 16055.0 61 13395.0 62 10735.0 63 9534.5 64 7128.5 65 5923.0 66 4877.0 67 3831.0 68 2954.5 69 2078.0 70 2140.5 71 2203.0 72 1311.5 73 420.0 74 350.0 75 280.0 76 168.0 77 56.0 78 35.5 79 15.0 80 18.5 81 22.0 82 11.0 83 0.0 84 0.5 85 1.0 86 0.5 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 52 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 52 312423.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 15.08339654890965 #Duplication Level Percentage of deduplicated Percentage of total 1 77.24089635854342 11.650550695691418 2 7.512095747389864 2.2661583814251833 3 2.6695526695526697 1.2079776456918985 4 1.1862320685850098 0.7156963475800437 5 0.6833036244800951 0.5153269765670261 6 0.49231813937696295 0.4455497834666462 7 0.36923860453272217 0.3898560605333154 8 0.29708853238265004 0.35848833152488774 9 0.29072234954587894 0.3946572435448094 >10 6.022408963585434 23.696398792662514 >50 1.9331975214328156 20.162087938468037 >100 1.2817248111365758 35.31174081293631 >500 0.012732365673542143 1.2118185921010938 >1k 0.00848824378236143 1.6736923978068197 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTAATGGGGATGGTGGTGGTGGTAGGGGTACGCTTAGGTGGGCAGTCCTGTG 1448 0.4634742000428906 No Hit GTAATGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1331 0.4260249725532371 No Hit GTAATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1275 0.4081005559769927 No Hit GTAATGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA 1175 0.3760926692336992 No Hit GTAATGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 765 0.24486033358619563 No Hit GTAATGGAGAGAAAAGGCTCCTGCCAGTGTGAAGACAGACGGACTGCTGTGA 755 0.2416595449118663 No Hit GTAATGGGGGAAACACTGTGTCCTTTGTTTAAGGGAAATGATTCATCTTTCT 604 0.19332763592949304 No Hit GTAATGGGGAGAAAGTGGCCACATATTTACAGTTAATGATCAATTCACATCT 589 0.188526452917999 No Hit GTAATGGGGGGCAGGGGAGATCTGGAAGGGAATGTCCAAAGAGAAGAAGGAG 566 0.18116463896704146 No Hit GTAATGGGGATAGAAGAGGTGGCCTTCATGGTGGAGATTGATCCAGGAACTA 507 0.16227998578849828 No Hit GTAATGGGGCGAAGGATGCCGCCGTGCTGTCTGTGGCCGCCCTCCCATGTCC 481 0.15395793523524198 No Hit GTAATGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGCCTGCACT 473 0.15139730429577847 No Hit GTAATGGGGGTACCTTGCTCTCTACGGTCTCTTTGGTTTCTGTCTGTAAGTT 441 0.14115478053792455 No Hit GTAATGGGGGAGTAGAATTTCCCTTCTGTCCCTTGTCACAAGTTTAAAAACC 423 0.1353933609241317 No Hit GTAATGGGGGTATTGGCCACCAACTGTAGATGTATATACGGTGTCCTTCTGA 409 0.1309122567800706 No Hit GTAATGGGGAGGCCAGGGTCTGGACTGCTCCGGCCCACAGGGCTGCCCAGCC 405 0.12963194131033887 No Hit GTAATGGGGATTGATGTGACGATGTGGGCAGACTCTCTGCATTTAGATGCCC 402 0.12867170470804007 No Hit GTAATGGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGTAATGGGGAAG 399 0.12771146810574124 No Hit GTAATGGGGATCTGGATAGTATGGTTTAAAAATATGAAAATATGTTAAGAAC 395 0.12643115263600951 No Hit GTAATGGGGAAGGGGCTCCCCCCTCTGCTCTGCACCAGCCCGCCCTGGGACC 386 0.1235504428291131 No Hit GTAATGGGGGCACATGGCTTGTGACGAATGAATCTTGCATTTTTTAGTGATC 385 0.12323036396168015 No Hit GTAATGGGGCAACTTTCAGGAACCAAGGACTGCCAGAAACTCTCTGCCTCTA 384 0.12291028509424723 No Hit GTAATGGGGTTTCTGGTCGGGGAGATTCCACATGCCTCGGGGCAGCTAAGCC 383 0.1225902062268143 No Hit GTAATGGGGTGCTGGCTCTTGTACCTTGGGAGATTGTAACAGTGACCAAATT 376 0.12034965415478374 No Hit GTAATGGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCAT 369 0.1181091020827532 No Hit GTAATGGGGGTAACACGGCAATTATTGTGCCTATTTTAGCCCACCGTGGGCT 361 0.1155484711432897 No Hit GTAATGGGGAAAATAACAATGTACTAGTCAGAATGGCCGTTCTAAAAGTCTA 359 0.11490831340842382 No Hit GTAATGGGGACCCTGTTACACACTCACACAGTTACCTCCCAGGCAGCCAGAT 354 0.11330791907125914 No Hit GTAATGGGGATAGCTTTCTTGTCCTTGTAAGAGGTGGATAAAGATACCCAGG 350 0.11202760360152743 No Hit GTAATGGGGATCCCTCCAGTGTAGCTCTTTTCTCTCCACCATGTAATTTTCC 349 0.11170752473409447 No Hit GTAATGGGTACCGCTGAAGACCTGCGCTAGGGCTGCTCAGCCCTCAGCCCTC 349 0.11170752473409447 No Hit GTAATGGGGAAGGTTTAAAAAAAGGAAAAAAAGTCTAGAGAAAAAAAAATAC 347 0.1110673669992286 No Hit GTAATGGGAGTTGTCTATGATGGGAAGAAAGGTACCAGGACCCCTGAGCTCT 347 0.1110673669992286 No Hit GTAATGGGGAGATGGCCCATTATTGAATCGAGTCGAAAACAAAGCACAAACC 343 0.10978705152949687 No Hit GTAATGGGGGAGAGGAAAATTCAAGATGGTCAAAGATGTCCTGCAGGATGTA 342 0.10946697266206393 No Hit GTAATGGGGGGATCTGCCATGGAGGAATCTGGTACCTCCAGATGGGTGCACA 342 0.10946697266206393 No Hit GTAATGGGGGTTCTCCCAAAAAGAGACCTCCCATGCTCAGGAAAAGTTGGGC 341 0.109146893794631 No Hit GTAATGGGGGGGCTTCCCAGGTGGAGGAGCTCCCCCATCTGGTGGTGCTTCT 340 0.10882681492719808 No Hit GTAATGGGGGTGGACTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCT 333 0.10658626285516752 No Hit GTAATGGGGGACTCTGTGCTTGTCTGTTTAGTTCTGTGTGTAAATGAAATGT 330 0.1056260262528687 No Hit GTAATGGGGCTGGGCCATTCTCTGCGCCTCTGGCTGGTTTTCATGAATTGAG 326 0.10434571078313697 No Hit GTAATGGGGAGGTGTGACCCATAGCTTTCTTGTCCTTGTAAGAGGTGGATAA 326 0.10434571078313697 No Hit GTAATGGGGGGGCAGACTGTCCATCCCAAGCCAGTTCAATTAGATGTAAATT 324 0.1037055530482711 No Hit GTAATGGGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 323 0.10338547418083815 No Hit GTAATGGGGAGTTCTATGAGATGACTGAGTACAGGTTTGCCTACTGGAGACC 322 0.10306539531340522 No Hit GTAATGGGGACTGGTTTGTCACTGTCACTGCGCCCAGTCACTCCCCACGTAT 319 0.10210515871110643 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.00640157734865871 0.0 0.0 0.0 0.0 8 0.009602366022988064 0.0 0.0 0.0 0.0 9 0.024325993924903096 0.0 0.0 0.0 0.0 10 0.06209530028198948 0.0 0.0 0.0 0.0 11 0.1110673669992286 0.0 0.0 0.0 0.0 12 0.14499572694711976 0.0 0.0 0.0 0.0 13 0.1696417997394558 0.0 0.0 0.0 0.0 14 0.19780874007355412 0.0 0.0 0.0 0.0 15 0.22149457626359134 0.0 0.0 0.0 0.0 16 0.2490213588628238 0.0 0.0 0.0 0.0 17 0.27302727392029397 0.0 0.0 0.0 0.0 18 0.2986335833149288 0.0 0.0 0.0 0.0 19 0.32455997157699656 0.0 0.0 0.0 0.0 20 0.35240683304366194 0.0 0.0 0.0 0.0 21 0.3834544831846567 0.0 0.0 0.0 0.0 22 0.41642260653024904 0.0 0.0 0.0 0.0 23 0.4519513608153049 0.0 0.0 0.0 0.0 24 0.4900407460398242 0.0 0.0 0.0 0.0 25 0.5178876075064895 0.0 0.0 0.0 0.0 26 0.5495754153823502 0.0 0.0 0.0 0.0 27 0.5796628289210461 0.0 0.0 0.0 0.0 28 0.6315156054451817 0.0 0.0 0.0 0.0 29 0.6532809684306213 0.0 0.0 0.0 0.0 30 0.679847514427555 0.0 0.0 0.0 0.0 31 0.6961715366666347 0.0 0.0 0.0 0.0 32 0.7134557955080132 0.0 0.0 0.0 0.0 33 0.7284995022773612 0.0 0.0 0.0 0.0 34 0.7419428147095445 0.0 0.0 0.0 0.0 35 0.7765113323923014 0.0 0.0 0.0 0.0 36 0.7883542504873201 0.0 0.0 0.0 0.0 37 0.798916853112607 0.0 0.0 0.0 0.0 38 0.8110798500750585 0.0 0.0 0.0 0.0 39 0.8264436357118394 0.0 0.0 0.0 0.0 40 0.8408471847463215 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTATGCG 55 1.8189894E-12 46.000004 34 ACGTTGA 55 1.8189894E-12 46.000004 15 TACGGTC 55 1.8189894E-12 46.000004 23 CCCACGT 55 1.8189894E-12 46.000004 44 TCTACGG 55 1.8189894E-12 46.000004 21 CTACGGT 55 1.8189894E-12 46.000004 22 CCACGTA 55 1.8189894E-12 46.000004 45 TCGCCAG 25 3.4114048E-5 46.0 36 CGGGTTC 25 3.4114048E-5 46.0 15 CTTCGGC 20 6.3043647E-4 46.0 38 GTTTGCG 25 3.4114048E-5 46.0 23 AGCGTTC 40 5.5879354E-9 46.0 15 AGCGTAC 25 3.4114048E-5 46.0 11 AATCCTC 25 3.4114048E-5 46.0 27 TAATACA 25 3.4114048E-5 46.0 15 ACTATCA 25 3.4114048E-5 46.0 22 TCGTTGC 20 6.3043647E-4 46.0 21 GATACCG 20 6.3043647E-4 46.0 9 CTCACGG 25 3.4114048E-5 46.0 11 CCTTAAG 40 5.5879354E-9 46.0 12 >>END_MODULE