FastQCFastQC Report
Fri 17 Jun 2016
SRR1527600_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527600_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7539
Sequences flagged as poor quality0
Sequence length50
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTAGAGGGCTCAAGCCAGATTGTGCTGCCAGAGAGCGAAAGGGTATGCG4125.464915771322457No Hit
CCTAGAGGGCAGAGTGCAGTGCTCCTAGGGGTCCTCCAAGCTTGAGACGT2503.316089667064597No Hit
CCTAGAGGGGTAATATCAGTATCGTATGCTTCAACAAATTTAGAATGACT1982.626343016315161No Hit
CCTAGAGGGCGGCACCCGCCGTGCACTGAATGCCAGCCCGTTGACGGTGC1862.4671707122960607No Hit
CCTAGAGGGTATATCATATTTGCATCAATAAATAGATCATCCAGACCAAA1471.9498607242339834No Hit
CCTAGAGGGCATCTCTACCAAAAAAATAAAATAAAGATTTAACTGAGTTT1371.8172171375513992No Hit
CCTAGAGGGGAGACAGGGTCTCACTATATAGCCCTGGATGTCCCAGAAGT1211.6049873988592651No Hit
CCTAGAGGGGAAAAAAATACATAGTAGAGATGCCCGGGAACCTGGAATAA1111.4723438121766812No Hit
CCTAGAGGGAGATTCTCATTGCTTTGTATCTTTCACGTTGTTTTCGCTGC1041.3794933014988726No Hit
CCTAGAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT951.2601140734845468No Hit
CCTAGAGGGGGAGGGGCTACGGCGGACATATCCCTCTTCCTGCAGCGTCT931.2335853561480303No Hit
CCTAGAGGGAGGACTTTAATAAGAATGAAAATAATGCTGGGTGCTGGTGG901.193792280143255No Hit
CCTAGAGGGGATCACCTCATTAGATGCTGAGAAAGCATTTGACAAACTAC821.087677410797188No Hit
CCTAGAGGGAGCCGCTGACCGTGCAGGCCGCCGCCATCTGCCAGGCACGC821.087677410797188No Hit
CCTAGAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT811.0744130521289297No Hit
CCTAGAGGGAGCCTTGTGTATATTTGTATATTATACATTTGTACAGAATT791.047884334792413No Hit
CCTAGACCTTTAGCAGCAAGGTCCATATCTGACTTTTTGTTAACGTATTT781.0346199761241544No Hit
CCTAGACAAATTAGAGCCAATACCATCAGCTTTACCGTCTTTCCAGAAAT771.021355617455896No Hit
CCTAGAGGGCGGGAGGCTGAGCACAAGAATCGCGGGAACCCAGGAGGTAG761.0080912587876376No Hit
CCTAGAGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCCTAGAGGGA690.9152407481098289No Hit
CCTAGAGGGATTGTGCACATGGGAAAGTTAAAGGATAAATTGTGAAGCCT690.9152407481098289No Hit
CCTAGAGGGGAGTCCCTTGATGGGTACAGTGGCTCACACCTGTAATCCCA630.8356545961002786No Hit
CCTAGAGGGCGATGGATAAATAAATCCCTGCACACCTCAAATGTGGGAGC620.8223902374320201No Hit
CCTAGAGGGGTCTGACTCGGGGGCGGACCGGCACATCGACATCCGGGAGT610.8091258787637617No Hit
CCTAGAGGGGGAGAAAGGGCAGACCCGCAGCGCGCTCCATCCTTTGCCCT600.7958615200955035No Hit
CCTAGAGGGATGGCTGTCAGCCAAAAGGAAGGCTCTGGAGCTGCCAAGAA570.7560684440907282No Hit
CCTAGAGGGATTTCAGTGGCTGCTACTCGGCGCTTCAGTCGCGGTCGCTT530.7030110094176947No Hit
CCTAGACGGGGGCGCTCGGGTTGCCTTGCTGGTGTTGGGGGGATAGTGTG530.7030110094176947No Hit
CCTAGAGGGGCTCTTCCGGGGACGGTGCTCAGAGGCACTCAGGATGGTCC510.6764822920811778No Hit
CCTAGAGGGGATAGGGGGAAAACCCAGCAGGCACTGACTGACCTGGCATA480.6366892160764027No Hit
CCTAGAGGGATGAGTGTTCTTTCTGCATGTATACCTGCATGTCAGAAGAG470.6234248574081444No Hit
CCTAGAGGGGCTAGGTGTGGTACTCACACTTACTGAGGATTTGTGTTATT450.5968961400716275No Hit
CCTAGAGGGGCTCCTAGAGGGAGCTTTCCATTACACAGCAGGCTGCTGTG450.5968961400716275No Hit
CCTAGAGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCC450.5968961400716275No Hit
CCTAGAGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCAT440.5836317814033691No Hit
CCTAGAGGGCTGCGGACTAGCGGTGGCTTCGCGTGGGTGGAGGAGGCGCG420.5571030640668524No Hit
CCTAGAGGGGCTTCCGGCGCGAAGGTCACGGACAAAATGGTGCCCCCGGT420.5571030640668524No Hit
CCTAGAGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCG410.543838705398594No Hit
CCTAGAGGGACCCCAGCCCCAGGTCTACTCTCTGGTCACAGCTTCACAGC400.5305743467303355No Hit
CCTAGAGGGGGTTGCACGGAGTTGTGAAGAGTACTGCCTTGTCCTTTGGC400.5305743467303355No Hit
CCTAGAGGGGAGACCCGCAGCGCGCTCCATCCTTTGCCCTTCAGTGCTGC390.5173099880620772No Hit
CCTAGAGGGCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATT390.5173099880620772No Hit
CCTAGAGGGGAGTTTAGTCTTGACCTTCAGGCCTGTTCTCAGTCCCTGGA370.4907812707255604No Hit
CCTAGAGGGGAGAGCATGAGGAGGTTGACTGAGTTCTGGCCTGTTTGAAA360.47751691205730207No Hit
CCTAGAGGGGGTAGGCAGATAAACAGCGGTGACAGGATTGTTTTGGTGCG360.47751691205730207No Hit
CCTAGAGGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCG350.4642525533890436No Hit
CCTAGAGGGGGCCCTCTCCCGTGGCCTCCCTGGGGTGGATCCTGCCTTGC340.4509881947207852No Hit
CCTAGAGGGGAATGCGAATCGGGAAGAGGGGATGAGCTGCGCTGCCGTGC330.4377238360525269No Hit
CCTAGAGGGGAGGAAAATACCAATAGTAATTAGCATCTTAATTACCTAGG330.4377238360525269No Hit
CCTAGAGGGACTTCCGGTTGGCGTCCCACGCGTGGTGCGCGGTGTTGTCT320.4244594773842684No Hit
CCTAGAGGGAAGTGACATTTGGAGATGACAGTCACACAGAGTGGTGGAAT320.4244594773842684No Hit
CCTAGAGGGACTCGTACAGGAGCTTAGGCATGGGTCGTGGCACCTTCTTC320.4244594773842684No Hit
CCTAGAGGGTTATGGCCAGATCATGCGCCACCTGGATAAGGTCGAGACAA320.4244594773842684No Hit
CCTAGAGGGATCGAGGGTTTCCTCCAGGCCCTATCTCTGGCAGTGGACAA310.41119511871601006No Hit
CCTAGAGGGGGCCTTTGAGAGCCATAATAGACATACTAAGTACTAATAAT310.41119511871601006No Hit
CCTAGAGGGACCAAAATAAAGATATGGAATTACGATTTTAAAGATAACTG300.39793076004775174No Hit
CCTAGAGGGGAGATCAGAGGAATTCATGGAACCAAAGAAACAGACACTGC300.39793076004775174No Hit
CCTAGAGGGGATGGGTGGATGGGACCGACCTTGACAAAGGTTTCAAGAAT290.3846664013794933No Hit
CCTAGAGGGGCTTTTCGACGCGGCGGAAGAGAAAATTGGGAAAGGCCGCG290.3846664013794933No Hit
CCTAGAGGGTCTGGCAAGCCAGGAACTGGCGCCAGATCAGCTCGTAGAGG290.3846664013794933No Hit
CCTAGAGGGGATCCGAATTCAGTCTTCCTGAGGCTCTGGCTCACCTCCAG270.3581376840429765No Hit
CCTAGAGGGATCCGCTGCAAGGGATGGACCCTGAGAGAACTGATACAGAA270.3581376840429765No Hit
CCTAGAGGGGCATTCAGCCTTTGGTGATCCTCCAGGACCTAAGGTTTCGG270.3581376840429765No Hit
CCTAGAGGGAGGGCTCTTGAGTCCGTGCAGGCACAATTCTAGTGCACGCG270.3581376840429765No Hit
CCTAGAGGGGCTGAGGACAGCAGCAGGCTCTGGGCTCTGGGAATCCACTG260.34487332537471815No Hit
CCTAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA250.33160896670645973No Hit
CCTAGAGGGAGCTCCTCTGACTTCAGTACCTCTGCTTTATAGTCAACCAC250.33160896670645973No Hit
CCTAGAGGGAGGTTGACAGTAAACAGCTTGAGATTTATTGCAGAACAAAA250.33160896670645973No Hit
CCTAGAGGGAGGAGAGAGGAAGACCCTAGGCAGATGGAGGACTTCGGCCA250.33160896670645973No Hit
CCTAGAGGGACACGAAGGGTGCCCTTCCCTTGGACACAGTCACTTTCTAC250.33160896670645973No Hit
CCTAGAGGGGGGCTCCACCCAAGAGGAAAAGAAAGAGAGTTAAACAGAGA250.33160896670645973No Hit
CCTAGAGGGATTTGGGGGAAGTGACTGCACCGGCAATTTCTGTTTGTTCA240.31834460803820136No Hit
CCTAGAGGGCGGTGATGTAAGACAGTTGTGAAATCCCCGGGCTCAACCTG240.31834460803820136No Hit
CCTAGAGGGGTACCATGTTGAATTGAGACAGCCTGACTGGGGGACTCAAG230.30508024936994294No Hit
CCTAGAGGGGAGTTCCGTTGTGGTCCTCTTCTTCACCACATGCCAACAAA220.29181589070168457No Hit
CCTAGAGGGGGTCCGGCAGCCGAGGAGGCCCCGCGCAGTGCTGCCAACGC220.29181589070168457No Hit
CCTAGAGGGGCAGTTCTGTCAGCGCAGCGCGATCACCAGCTCCCAGCGCC220.29181589070168457No Hit
CCTAGAGGGCTTTCTCTGTGATAACCCCAGCTGTGTCAAGTTGACACAAA220.29181589070168457No Hit
CCTAGAGGGGGTGGAGGTTCCAGTGAGCCGAGATCACGCCATTGCACTCC210.2785515320334262No Hit
CCTAGAGGGAGGCGCGCGCGCGCGCGCACACACACACACACACACACACA210.2785515320334262No Hit
CCTAGAGGGGCCCACTCCACCCAGGCTCGGTCGTCGGAAACGCTTTCTGA210.2785515320334262No Hit
CCTAGAGGGGATGGAGGTGGGGGGGCCTGTGTGATGTGTGCTCCTTCCCT200.26528717336516777No Hit
CCTAGAGGGGCGATCTCCGAGGCCGCATACATATTACCCACAATTCCCTT200.26528717336516777No Hit
CCTAGAGGGAAGGTGACATTCCGTTGTTTCAGCACCAATCCAACTTAAAA200.26528717336516777No Hit
CCTAGAGGGGCCCCACTGGCTGCTCCGAAAAGCCATCTTTGCATTGTTCC200.26528717336516777No Hit
CCTAGAGGGCCTAAGTCTCGAGACGGAGTTCCACCGCTCTGCCCACTCCT200.26528717336516777No Hit
CCTAGAGGGGACAATGGTGTCTTTGAAGTGGTGGCCACTAATGGAGATAC190.2520228146969094No Hit
CCTAGAGGGGTGATTTCCTGGATTCTCAGGACTCGGGCTCGCTGTGCTTC190.2520228146969094No Hit
CCTAGAGGGGGCTGGCCTGCACTTAAGAGCCCTAGACCCATTTCCTCAAG190.2520228146969094No Hit
CCTAGAGGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA190.2520228146969094No Hit
CCTAGAGGGATAGTTCATCCTTCTCAGATCTCTGATGACATTGACGGCTG190.2520228146969094No Hit
CCTAGAGGGGAGCAGCAGGCTCTGGGCTCTGGGAATCCACTGCTGTCCGG180.23875845602865103No Hit
CCTAGAGGGGGTCTCAACCCTGTTATTTGGCTGTGTTGGGGCTTGTCCTG180.23875845602865103No Hit
CCTAGAGGGGCTGTATCCTTGGTTATCAATAAAATATTTCCTGGCAAAAA170.2254940973603926No Hit
CCTAGAGGGATCTTAAATAACAATCAAGATAAATACCTCACAGACATGCT170.2254940973603926No Hit
CCTAGAGGGGGGGTGTTAGATCCCATTACAGATGGTTGTGAGCCACCATG170.2254940973603926No Hit
CCTAGAGGGTCTCTGGTTTTTAACTCTTTGCTGTGGAGTTTAAAGTTGGT170.2254940973603926No Hit
CCTAGAGGGGAAGAACAGAGTGGAAGCCTCCATCACCAAGGCAAACTCAT160.2122297386921342No Hit
CCTAGAGGGGAACGGCTGCAGGAACAGAAAGACAGGAAGCAGCAGGAGTA160.2122297386921342No Hit
CCTAGAGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCAC160.2122297386921342No Hit
CCTAGACGGGGTGCAGTGCTCCAGACGGGGAAGAGAAATGTGGAACACTG160.2122297386921342No Hit
CCTAGAGGGGTGCTCCTAGAGGGAGAAACATCCAAGGCCAGACTGGCACC160.2122297386921342No Hit
CCTAGAGGGGCAGAGAAGGATGAATTGTAAATTATACTCTCGCTATGAAT160.2122297386921342No Hit
CCTAGAGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACA150.19896538002387587No Hit
CCTAGAGGGGTAATATCAGTATCGTATGCCGTCTTCTGCTTGAAAAAAAA150.19896538002387587TruSeq Adapter, Index 15 (96% over 27bp)
CCTAGAGGGAGACGTTCTCGCCCAGAGTCGCCGCGGTTTCCTGCTTCAAC150.19896538002387587No Hit
CCTAGAGGGGCCGATGTGGGTGAAGAACTGCTCGATACGTTCCAGAGTCC150.19896538002387587No Hit
CCTAGAGGGCATTTCCCTTCTTCAGAGGTGTAGTGTCCTGGCTCTTGGCA150.19896538002387587No Hit
CCTAGAGGGGGAAAAAAAATTACATCATTTGTTAAGTATAGCAGCTCACA150.19896538002387587No Hit
CCTAGAGGGATGTATGTTTTTCCATAATATTTCCAGTTATGTGTATTTAA140.18570102135561745No Hit
CCTAGAGGGATTCATGCTGATTATAATACAGAGGACTTATTTCTAAATGG140.18570102135561745No Hit
CCTAGAGGGAGGGGTGAAGCTTCCTGGGGGTTTGGAACCCAAGGGGGAGC140.18570102135561745No Hit
CCTAGAGGGGCATTATTTTTATTTACTTTTTTGTAAAGTGATTTCCAGTC140.18570102135561745No Hit
CCTAGACACCCACCTGGCCCAAGGCTAGCTCCTATCAGAGTTATAGTCAG140.18570102135561745No Hit
CCTAGAGGGGGAGCCACACGAAACGCCAACACCGCCACCGGACAACAACA140.18570102135561745No Hit
CCTAGAGGGGAAGGAATCAAATTTAGAACCAAAGCCTAATTTACCACCAG130.17243666268735908No Hit
CCTAGAGGGGATTCTGTAGTAACTCAATTAACCATGGGCGATGGTGACTC130.17243666268735908No Hit
CCTAGAGGGGCACTCGGAGTAAACGCGGCTGGCGGTAGTACGTGTGTCTT120.15917230401910068No Hit
CCTAGAGGGGCCTTTCCGGCGGTGACGACCTCCCTACGAGAACATGCCTC120.15917230401910068No Hit
CCTAGAGGGGACGCTCAGAGCTTGAGGTCCTTGTTGCTTGTGGGTCGGGT120.15917230401910068No Hit
CCTAGAGGGCCCTCCCGGTCAAACGGGAGGGTAGCCCTGAAGGGCTATGA120.15917230401910068No Hit
CCTAGAGGGGATTAGACGGTTGCGGGCGCAGAGGGTTGGTAGTTGTCGCT120.15917230401910068No Hit
CCTAGAGGGGATTTCAGTGGCTGCTACTCGGCGCTTCAGTCGCGGTCGCT120.15917230401910068No Hit
CCTAGAGGGGCAGAGTGCAGTACTCCTAGAGGGGTCGAGAGAAGAGGTCA110.14590794535084228No Hit
CCTAGAGGGGGGAGTGCAGTACTCCTAGAGGGGGGAGTGCAGTACTCCTA110.14590794535084228No Hit
CCTAGAGGGGGTGGTGCTCCTGATGTCCCTCACCCACCCCTGAAGATCCC110.14590794535084228No Hit
CCTAGAGGGGATTTTTCAGTGGCTGCTACTCGGCGCTTCAGTCGCGGTCG110.14590794535084228No Hit
CCTAGACGGGAGCATTTCAATATACTCCTACCTCCCGGCATTTAAAGCGT110.14590794535084228No Hit
CCTAGAGGGGATCTTGTTAAATTTTTTATATTTATTGATAAGATAATATA110.14590794535084228No Hit
CCTAGAGGGCGAGCGACTAAAGTGCGCCGAATCCGTAAACCCCCATTTAT110.14590794535084228No Hit
CCTAGAGGGGGGGGACAGAGCCAATTTCAAGTCATGGCCAAAATTTTGTA110.14590794535084228No Hit
CCTAGAGGGATTCGCTCCTTGTTGCGTGCACCCAAGCAAGGTTTTATTTT110.14590794535084228No Hit
CCTAGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGT100.13264358668258389No Hit
CCTAGAGGGACTTTACAGAAAAAGGAAAAAGTTTAGGAAACAACCAAGAT100.13264358668258389No Hit
CCTAGAGGGGCGACGAAAAAATGCCTTGCAAAAAAACAAGATGATACGAA100.13264358668258389No Hit
CCTAGAGGGGATTCAGCCTTTGGTGATCCTCCAGGACCTAAGGTTTCGGT100.13264358668258389No Hit
CCTAGAGGGAAGGACCAATTTGAAAGGTAAAATAAAAAAAAAAAAAAAAA100.13264358668258389No Hit
CCTAGAGGGGAGACAGGGTCTCACTATATAGCCCTGGATGTCACAGAAGT90.11937922801432552No Hit
CCTAGAGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCA90.11937922801432552No Hit
CCTAGAGGGAGTAATGAAGTCCAACCCCAAGTTTTGATTTTTCTCGAAAA90.11937922801432552No Hit
CCTAGAGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGCTAGAGGGA90.11937922801432552No Hit
CCTAGAGGGCAGTGCTCCTAGAGGGAGAGTGCAGTGCTTAAGGGAAAAAA90.11937922801432552No Hit
CCTAGAGGGAAGCAGTGGTATCAACGCAGATCGGAAGAGCTCGTATGCCG80.1061148693460671Illumina Single End Adapter 2 (100% over 23bp)
CCTAGAGGGGCAGCAGGGCTGAGTACCAGCGCATCCCGGACAGTGCCATC80.1061148693460671No Hit
CCTAGAGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA80.1061148693460671No Hit
CCTAGAGGGATCCCACCACCGTATCCCACCCCACCACACCTGTGTAGTTG80.1061148693460671No Hit
CCTAGAGGGGCCCCCTTCTCCTCTTTCCCTTTCCTCTGCTCATTGTTGAC80.1061148693460671No Hit
CCTAGAGGGGAGACACACAGCAGCAGCAGCAGCAGCAGCAGCAGCAACAG80.1061148693460671No Hit
CCTAGAGGGCCACGTGTCATTGCTTTCCTAGAGTGACTCACATGCAGAGC80.1061148693460671No Hit
CCTAGAGGGGTCTGTTCTGCACCAACTCAGAGTCTTGTTGGAGCTTTATC80.1061148693460671No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGAGACG254.0330928E-544.043
TTAGAAT254.0330928E-544.040
GGAGATT207.366297E-444.08
AGGGGTA302.2070108E-644.06
CTTTCAC207.366297E-444.030
TTGTTTT207.366297E-444.038
AGGGGCA254.0330928E-544.06
CTTCAAC302.2070108E-644.029
AGGGGAG600.044.06
AGGGGAA453.583409E-1044.06
TTCACGT207.366297E-444.032
AACGCAG207.366297E-444.023
CTGAGTT207.366297E-444.043
ATCTCTA254.0330928E-544.011
ATCTTTC207.366297E-444.028
GGGCATC254.0330928E-544.07
GGGTCCT254.0330928E-544.029
AACAAAT254.0330928E-544.033
CATTGCT207.366297E-444.017
GGCATCT254.0330928E-544.08
Produced by FastQC (version 0.11.5)