FastQCFastQC Report
Fri 17 Jun 2016
SRR1527599_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527599_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7786
Sequences flagged as poor quality0
Sequence length52
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTAGAGGGCTCAAGCCAGATTGTGCTGCCAGAGAGCGAAAGGGTATGCGCG4335.561263806832777No Hit
CCTAGAGGGGTAATATCAGTATCGTATGCTTCAACAAATTTAGAATGACTTC2092.6843051631132804No Hit
CCTAGAGGGCAGAGTGCAGTGCTCCTAGGGGTCCTCCAAGCTTGAGACGTCT2052.6329309016182894No Hit
CCTAGAGGGCGGCACCCGCCGTGCACTGAATGCCAGCCCGTTGACGGTGCTG2022.594400205497046No Hit
CCTAGAGGGCATCTCTACCAAAAAAATAAAATAAAGATTTAACTGAGTTTGG1702.1834061135371177No Hit
CCTAGAGGGGAGACAGGGTCTCACTATATAGCCCTGGATGTCCCAGAAGTAG1411.8109427176984332No Hit
CCTAGAGGGTATATCATATTTGCATCAATAAATAGATCATCCAGACCAAAAA1361.7467248908296942No Hit
CCTAGAGGGGGAGGGGCTACGGCGGACATATCCCTCTTCCTGCAGCGTCTCC1311.6825070639609558No Hit
CCTAGAGGGAGATTCTCATTGCTTTGTATCTTTCACGTTGTTTTCGCTGCTA1021.3100436681222707No Hit
CCTAGAGGGGATCACCTCATTAGATGCTGAGAAAGCATTTGACAAACTACAA1011.297200102748523No Hit
CCTAGAGGGAGGACTTTAATAAGAATGAAAATAATGCTGGGTGCTGGTGGTG991.2715129720010274No Hit
CCTAGAGGGATTGTGCACATGGGAAAGTTAAAGGATAAATTGTGAAGCCTGG981.2586694066272797No Hit
CCTAGAGGGGAAAAAAATACATAGTAGAGATGCCCGGGAACCTGGAATAATA931.194451579758541No Hit
CCTAGAGGGAGCCTTGTGTATATTTGTATATTATACATTTGTACAGAATTTT931.194451579758541No Hit
CCTAGAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG931.194451579758541No Hit
CCTAGAGGGCGGGAGGCTGAGCACAAGAATCGCGGGAACCCAGGAGGTAGAG871.1173901875160546No Hit
CCTAGAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC861.1045466221423068No Hit
CCTAGACCTTTAGCAGCAAGGTCCATATCTGACTTTTTGTTAACGTATTTAG811.040328795273568No Hit
CCTAGACAAATTAGAGCCAATACCATCAGCTTTACCGTCTTTCCAGAAATTG791.0146416645260725No Hit
CCTAGAGGGCGATGGATAAATAAATCCCTGCACACCTCAAATGTGGGAGCGG791.0146416645260725No Hit
CCTAGAGGGAGCCGCTGACCGTGCAGGCCGCCGCCATCTGCCAGGCACGCGG770.9889545337785769No Hit
CCTAGAGGGGCTCTTCCGGGGACGGTGCTCAGAGGCACTCAGGATGGTCCAG760.9761109684048292No Hit
CCTAGAGGGATGGCTGTCAGCCAAAAGGAAGGCTCTGGAGCTGCCAAGAAAG660.8476753146673517No Hit
CCTAGACGGGGGCGCTCGGGTTGCCTTGCTGGTGTTGGGGGGATAGTGTGTG610.7834574877986129No Hit
CCTAGAGGGGGAGAAAGGGCAGACCCGCAGCGCGCTCCATCCTTTGCCCTTC610.7834574877986129No Hit
CCTAGACACCCACCTGGCCCAAGGCTAGCTCCTATCAGAGTTATAGTCAGCG580.7449267916773696No Hit
CCTAGAGGGGCCCCACTGGCTGCTCCGAAAAGCCATCTTTGCATTGTTCCTG570.7320832263036219No Hit
CCTAGAGGGATCCGCTGCAAGGGATGGACCCTGAGAGAACTGATACAGAAAG570.7320832263036219No Hit
CCTAGAGGGATTTCAGTGGCTGCTACTCGGCGCTTCAGTCGCGGTCGCTTCA570.7320832263036219No Hit
CCTAGAGGGACCCCAGCCCCAGGTCTACTCTCTGGTCACAGCTTCACAGCCA550.7063960955561264No Hit
CCTAGAGGGGAATGCGAATCGGGAAGAGGGGATGAGCTGCGCTGCCGTGCGT500.6421782686873876No Hit
CCTAGAGGGGAGTCCCTTGATGGGTACAGTGGCTCACACCTGTAATCCCAGC490.6293347033136398No Hit
CCTAGAGGGGAGACCCGCAGCGCGCTCCATCCTTTGCCCTTCAGTGCTGCCT470.6036475725661443No Hit
CCTAGAGGGGCTAGGTGTGGTACTCACACTTACTGAGGATTTGTGTTATTTC470.6036475725661443No Hit
CCTAGAGGGGAGTTTAGTCTTGACCTTCAGGCCTGTTCTCAGTCCCTGGACT470.6036475725661443No Hit
CCTAGAGGGATGAGTGTTCTTTCTGCATGTATACCTGCATGTCAGAAGAGAG460.5908040071923966No Hit
CCTAGAGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGG460.5908040071923966No Hit
CCTAGAGGGGGTTGCACGGAGTTGTGAAGAGTACTGCCTTGTCCTTTGGCGT460.5908040071923966No Hit
CCTAGAGGGGAGAGCATGAGGAGGTTGACTGAGTTCTGGCCTGTTTGAAAGA460.5908040071923966No Hit
CCTAGAGGGGCTTCCGGCGCGAAGGTCACGGACAAAATGGTGCCCCCGGTTC450.5779604418186489No Hit
CCTAGAGGGGATAGGGGGAAAACCCAGCAGGCACTGACTGACCTGGCATATC440.565116876444901No Hit
CCTAGAGGGATTTGGGGGAAGTGACTGCACCGGCAATTTCTGTTTGTTCAAG420.5394297456974056No Hit
CCTAGAGGGGGCCTTTGAGAGCCATAATAGACATACTAAGTACTAATAATAA420.5394297456974056No Hit
CCTAGAGGGCTGCGGACTAGCGGTGGCTTCGCGTGGGTGGAGGAGGCGCGGC410.5265861803236578No Hit
CCTAGAGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCCTAGAGGGAAG410.5265861803236578No Hit
CCTAGAGGGGCTCCTAGAGGGAGCTTTCCATTACACAGCAGGCTGCTGTGTA390.5008990495761623No Hit
CCTAGAGGGCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTTT380.4880554842024146No Hit
CCTAGAGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT370.4752119188286668No Hit
CCTAGAGGGACTTCCGGTTGGCGTCCCACGCGTGGTGCGCGGTGTTGTCTCT370.4752119188286668No Hit
CCTAGAGGGGATGGGTGGATGGGACCGACCTTGACAAAGGTTTCAAGAATTG370.4752119188286668No Hit
CCTAGAGGGGGTAGGCAGATAAACAGCGGTGACAGGATTGTTTTGGTGCGCA370.4752119188286668No Hit
CCTAGAGGGGCATTCAGCCTTTGGTGATCCTCCAGGACCTAAGGTTTCGGTA360.46236835345491906No Hit
CCTAGAGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCA350.44952478808117136No Hit
CCTAGAGGGGTCTGACTCGGGGGCGGACCGGCACATCGACATCCGGGAGTCT350.44952478808117136No Hit
CCTAGAGGGAGGTTGACAGTAAACAGCTTGAGATTTATTGCAGAACAAAATC340.43668122270742354No Hit
CCTAGAGGGGGCCCTCTCCCGTGGCCTCCCTGGGGTGGATCCTGCCTTGCGA340.43668122270742354No Hit
CCTAGAGGGGCTGAGGACAGCAGCAGGCTCTGGGCTCTGGGAATCCACTGCT330.42383765733367584No Hit
CCTAGAGGGGAGGAAAATACCAATAGTAATTAGCATCTTAATTACCTAGGAT330.42383765733367584No Hit
CCTAGAGGGGCCCACTCCACCCAGGCTCGGTCGTCGGAAACGCTTTCTGAGT330.42383765733367584No Hit
CCTAGAGGGACACGAAGGGTGCCCTTCCCTTGGACACAGTCACTTTCTACAA320.4109940919599281No Hit
CCTAGAGGGGACAATGGTGTCTTTGAAGTGGTGGCCACTAATGGAGATACTC320.4109940919599281No Hit
CCTAGAGGGAAGTGACATTTGGAGATGACAGTCACACAGAGTGGTGGAATCG320.4109940919599281No Hit
CCTAGAGGGAGGAGAGAGGAAGACCCTAGGCAGATGGAGGACTTCGGCCACA320.4109940919599281No Hit
CCTAGAGGGGATGGAGGTGGGGGGGCCTGTGTGATGTGTGCTCCTTCCCTGA310.3981505265861803No Hit
CCTAGAGGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAG290.3724633958386848No Hit
CCTAGAGGGCGGTGATGTAAGACAGTTGTGAAATCCCCGGGCTCAACCTGGG290.3724633958386848No Hit
CCTAGAGGGATCGAGGGTTTCCTCCAGGCCCTATCTCTGGCAGTGGACAAAC280.35961983046493706No Hit
CCTAGAGGGAGGGCTCTTGAGTCCGTGCAGGCACAATTCTAGTGCACGCGAC280.35961983046493706No Hit
CCTAGAGGGTCTGGCAAGCCAGGAACTGGCGCCAGATCAGCTCGTAGAGGCG270.3467762650911893No Hit
CCTAGAGGGGATCCGAATTCAGTCTTCCTGAGGCTCTGGCTCACCTCCAGGC260.33393269971744155No Hit
CCTAGAGGGGTAATATCAGTATCGTATGCCGTCTTCTGCTTGAAAAAAAAAA260.33393269971744155TruSeq Adapter, Index 15 (96% over 27bp)
CCTAGAGGGTTATGGCCAGATCATGCGCCACCTGGATAAGGTCGAGACAAAA250.3210891343436938No Hit
CCTAGAGGGGAGATCAGAGGAATTCATGGAACCAAAGAAACAGACACTGCAA250.3210891343436938No Hit
CCTAGAGGGACCAAAATAAAGATATGGAATTACGATTTTAAAGATAACTGAC250.3210891343436938No Hit
CCTAGAGGGGCAGTTCTGTCAGCGCAGCGCGATCACCAGCTCCCAGCGCCGC250.3210891343436938No Hit
CCTAGACGGGGTGCAGTGCTCCAGACGGGGAAGAGAAATGTGGAACACTGGA240.3082455689699461No Hit
CCTAGAGGGATCTTAAATAACAATCAAGATAAATACCTCACAGACATGCTCT240.3082455689699461No Hit
CCTAGAGGGGTACCATGTTGAATTGAGACAGCCTGACTGGGGGACTCAAGTG240.3082455689699461No Hit
CCTAGAGGGGTGCTCCTAGAGGGAGAAACATCCAAGGCCAGACTGGCACCCA240.3082455689699461No Hit
CCTAGAGGGATTCATGCTGATTATAATACAGAGGACTTATTTCTAAATGGAG240.3082455689699461No Hit
CCTAGAGGGGAGTTCCGTTGTGGTCCTCTTCTTCACCACATGCCAACAAAGT230.2954020035961983No Hit
CCTAGAGGGGGTGGTGCTCCTGATGTCCCTCACCCACCCCTGAAGATCCCAG230.2954020035961983No Hit
CCTAGAGGGGCCGATGTGGGTGAAGAACTGCTCGATACGTTCCAGAGTCCGG230.2954020035961983No Hit
CCTAGAGGGACTCGTACAGGAGCTTAGGCATGGGTCGTGGCACCTTCTTCCC230.2954020035961983No Hit
CCTAGAGGGGATCTTGTTAAATTTTTTATATTTATTGATAAGATAATATATT220.2825584382224505No Hit
CCTAGAGGGAGCTCCTCTGACTTCAGTACCTCTGCTTTATAGTCAACCACAG210.2697148728487028No Hit
CCTAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA210.2697148728487028No Hit
CCTAGAGGGAGACGTTCTCGCCCAGAGTCGCCGCGGTTTCCTGCTTCAACAG210.2697148728487028No Hit
CCTAGAGGGGGGGTGTTAGATCCCATTACAGATGGTTGTGAGCCACCATGTG210.2697148728487028No Hit
CCTAGAGGGGGTCTCAACCCTGTTATTTGGCTGTGTTGGGGCTTGTCCTGCT200.25687130747495507No Hit
CCTAGAGGGATCCCACCACCGTATCCCACCCCACCACACCTGTGTAGTTGAG200.25687130747495507No Hit
CCTAGAGGGGCTTTTCGACGCGGCGGAAGAGAAAATTGGGAAAGGCCGCGAA200.25687130747495507No Hit
CCTAGAGGGCCCTCCCGGTCAAACGGGAGGGTAGCCCTGAAGGGCTATGAAA200.25687130747495507No Hit
CCTAGAGGGGCCTTTCCGGCGGTGACGACCTCCCTACGAGAACATGCCTCTC190.2440277421012073No Hit
CCTAGAGGGATAGTTCATCCTTCTCAGATCTCTGATGACATTGACGGCTGTC190.2440277421012073No Hit
CCTAGAGGGAATTGACTTCTAGTGTAGCATTATTAGTTTGCTAAATATGTAC190.2440277421012073No Hit
CCTAGAGGGGCGATCTCCGAGGCCGCATACATATTACCCACAATTCCCTTTC190.2440277421012073No Hit
CCTAGAGGGGATTAGACGGTTGCGGGCGCAGAGGGTTGGTAGTTGTCGCTGT190.2440277421012073No Hit
CCTAGAGGGGCATTATTTTTATTTACTTTTTTGTAAAGTGATTTCCAGTCTT190.2440277421012073No Hit
CCTAGAGGGGTGATTTCCTGGATTCTCAGGACTCGGGCTCGCTGTGCTTCTG180.23118417672745953No Hit
CCTAGAGGGGATTTTTCAGTGGCTGCTACTCGGCGCTTCAGTCGCGGTCGCT180.23118417672745953No Hit
CCTAGAGGGATTCGCTCCTTGTTGCGTGCACCCAAGCAAGGTTTTATTTTCT180.23118417672745953No Hit
CCTAGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC170.21834061135371177No Hit
CCTAGAGGGCTTTCTCTGTGATAACCCCAGCTGTGTCAAGTTGACACAAAAC160.20549704597996404No Hit
CCTAGAGGGGCACTCGGAGTAAACGCGGCTGGCGGTAGTACGTGTGTCTTTT160.20549704597996404No Hit
CCTAGAGGGGGTGGAGGTTCCAGTGAGCCGAGATCACGCCATTGCACTCCAG160.20549704597996404No Hit
CCTAGAGGGGGAGCCACACGAAACGCCAACACCGCCACCGGACAACAACAAG160.20549704597996404No Hit
CCTAGAGGGCCTAAGTCTCGAGACGGAGTTCCACCGCTCTGCCCACTCCTGC150.1926534806062163No Hit
CCTAGAGGGTCTCTGGTTTTTAACTCTTTGCTGTGGAGTTTAAAGTTGGTTA150.1926534806062163No Hit
CCTAGAGGGCATTTCCCTTCTTCAGAGGTGTAGTGTCCTGGCTCTTGGCAAG150.1926534806062163No Hit
CCTAGAGGGAGGGGTGAAGCTTCCTGGGGGTTTGGAACCCAAGGGGGAGCTG150.1926534806062163No Hit
CCTAGAGGGGAACGGCTGCAGGAACAGAAAGACAGGAAGCAGCAGGAGTATG150.1926534806062163No Hit
CCTAGAGGGGGTCCGGCAGCCGAGGAGGCCCCGCGCAGTGCTGCCAACGCCC140.17980991523246853No Hit
CCTAGAGGGATCCAGTGCATGCATGAGGTGCACACGTTCGTGTCCACGTGCC140.17980991523246853No Hit
CCTAGAGGGAAGGTGACATTCCGTTGTTTCAGCACCAATCCAACTTAAAAGA140.17980991523246853No Hit
CCTAGAGGGGGCTGGCCTGCACTTAAGAGCCCTAGACCCATTTCCTCAAGGT140.17980991523246853No Hit
CCTAGAGGGGGGGGACAGAGCCAATTTCAAGTCATGGCCAAAATTTTGTAAG140.17980991523246853No Hit
CCTAGAGGGAGGCGCGCGCGCGCGCGCACACACACACACACACACACACACA140.17980991523246853No Hit
CCTAGAGGGAAGGACCAATTTGAAAGGTAAAATAAAAAAAAAAAAAAAAAAA140.17980991523246853No Hit
CCTAGAGGGGATTCAGCCTTTGGTGATCCTCCAGGACCTAAGGTTTCGGTAT140.17980991523246853No Hit
CCTAGAGGGGGGCTCCACCCAAGAGGAAAAGAAAGAGAGTTAAACAGAGAAT140.17980991523246853No Hit
CCTAGAGGGGAGTCGTCGTCGCCGCTGCCACTGCCCGTGTGTGCCGCGTCAG130.16696634985872078No Hit
CCTAGAGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAAC130.16696634985872078No Hit
CCTAGAGGGGATAACTGAGGCGGAAGCATAGCTTGTGGAGCTGGGCCGCGTT120.15412278448497305No Hit
CCTAGAGGGGAGCAGCAGGCTCTGGGCTCTGGGAATCCACTGCTGTCCGGCC120.15412278448497305No Hit
CCTAGAGGGGCTGTATCCTTGGTTATCAATAAAATATTTCCTGGCAAAAAAA120.15412278448497305No Hit
CCTAGAGGGGAAGAACAGAGTGGAAGCCTCCATCACCAAGGCAAACTCATTC120.15412278448497305No Hit
CCTAGAGGGCAGTGCTCCTAGAGGGAGAGTGCAGTGCTTAAGGGAAAAAAAA120.15412278448497305No Hit
CCTAGAGGGGACGCTCAGAGCTTGAGGTCCTTGTTGCTTGTGGGTCGGGTCC110.14127921911122526No Hit
CCTAGAGGGGCCCCCTTCTCCTCTTTCCCTTTCCTCTGCTCATTGTTGACAG110.14127921911122526No Hit
CCTAGAGGGATGTATGTTTTTCCATAATATTTCCAGTTATGTGTATTTAAGA110.14127921911122526No Hit
CCTAGAGGGGGAAAAAAAATTACATCATTTGTTAAGTATAGCAGCTCACACC100.12843565373747753No Hit
CCTAGAGGGAGGCTGCAATGAGCCGAGATCATGCCACTGCACTCCAGCCTGG100.12843565373747753No Hit
CCTAGAGGGACTTTACAGAAAAAGGAAAAAGTTTAGGAAACAACCAAGATAT100.12843565373747753No Hit
CCTAGAGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACA100.12843565373747753No Hit
CCTAGAGGGGCGACGAAAAAATGCCTTGCAAAAAAACAAGATGATACGAAAA90.11559208836372976No Hit
CCTAGAGGGGGGAGTGCAGTACTCCTAGAGGGGGGAGTGCAGTACTCCTAGA90.11559208836372976No Hit
CCTAGAGGGAAGCAGTGGTATCAACGCAGATCGGAAGAGCTCGTATGCCGTC90.11559208836372976Illumina Single End Adapter 2 (100% over 25bp)
CCTAGAGGGACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA90.11559208836372976No Hit
CCTAGAGGGGAGACCCGCAGCGCGCTCCATCCTTTGCCCTTCAGTGCTACCT90.11559208836372976No Hit
CCTAGAGGGCCACGTGTCATTGCTTTCCTAGAGTGACTCACATGCAGAGCAG80.10274852298998202No Hit
CCTAGAGGGCGAGCGACTAAAGTGCGCCGAATCCGTAAACCCCCATTTATAC80.10274852298998202No Hit
CCTAGACACCCACCTGGCCCAAGGCTAACTCCTATCAGAGTTATAGTCAGCG80.10274852298998202No Hit
CCTAGAGGGAGAGTGCAGTGCTCCTAGAGGGGTGGTATCAATGCAGAGTCGA80.10274852298998202No Hit
CCTAGAGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACAG80.10274852298998202No Hit
CCTAGAGGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA80.10274852298998202No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGCCCG205.929571E-446.00000434
AGCCCGT205.929571E-446.00000435
TGCACTG205.929571E-446.00000422
AGGGGCC205.929571E-446.0000046
TTGCATC404.476533E-946.00000420
AGGGGAA404.476533E-946.0000046
GTTGACG205.929571E-446.00000440
TGCATCA404.476533E-946.00000421
TATATTT205.929571E-446.00000419
TATATTA205.929571E-446.00000427
GTGCACT205.929571E-446.00000421
TAAATAG404.476533E-946.00000429
CTTTAAT205.929571E-446.00000414
TTGTATA205.929571E-446.00000424
TATACAT205.929571E-446.00000432
TATATCA404.476533E-946.00000410
ACAGAAT205.929571E-446.00000443
CTGGGTG205.929571E-446.00000437
ATAAGAA205.929571E-446.00000419
ACTGAAT205.929571E-446.00000425
Produced by FastQC (version 0.11.5)