FastQCFastQC Report
Fri 17 Jun 2016
SRR1527598_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527598_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7523
Sequences flagged as poor quality0
Sequence length52
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTAGAGGGCTCAAGCCAGATTGTGCTGCCAGAGAGCGAAAGGGTATGCGCG4165.529708892728964No Hit
CCTAGAGGGCAGAGTGCAGTGCTCCTAGGGGTCCTCCAAGCTTGAGACGTCT3164.200451947361425No Hit
CCTAGAGGGCGGCACCCGCCGTGCACTGAATGCCAGCCCGTTGACGGTGCTG2653.52253090522398No Hit
CCTAGAGGGGTAATATCAGTATCGTATGCTTCAACAAATTTAGAATGACTTC2433.230094377243121No Hit
CCTAGAGGGTATATCATATTTGCATCAATAAATAGATCATCCAGACCAAAAA1712.2730293765784926No Hit
CCTAGAGGGCATCTCTACCAAAAAAATAAAATAAAGATTTAACTGAGTTTGG1672.219859098763791No Hit
CCTAGAGGGGGAGGGGCTACGGCGGACATATCCCTCTTCCTGCAGCGTCTCC1441.9141300013292568No Hit
CCTAGAGGGGAGACAGGGTCTCACTATATAGCCCTGGATGTCCCAGAAGTAG1321.7546191678851524No Hit
CCTAGAGGGAGATTCTCATTGCTTTGTATCTTTCACGTTGTTTTCGCTGCTA1151.5286454871726705No Hit
CCTAGAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG1101.4621826399042934No Hit
CCTAGAGGGGAAAAAAATACATAGTAGAGATGCCCGGGAACCTGGAATAATA1071.4223049315432672No Hit
CCTAGAGGGGATCACCTCATTAGATGCTGAGAAAGCATTTGACAAACTACAA951.2627940980991625No Hit
CCTAGAGGGAGCCTTGTGTATATTTGTATATTATACATTTGTACAGAATTTT831.1032832646550579No Hit
CCTAGAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC761.01023527847933No Hit
CCTAGAGGGAGCCGCTGACCGTGCAGGCCGCCGCCATCTGCCAGGCACGCGG680.9038947228499269No Hit
CCTAGAGGGCGGGAGGCTGAGCACAAGAATCGCGGGAACCCAGGAGGTAGAG640.8507244450352253No Hit
CCTAGAGGGAGGACTTTAATAAGAATGAAAATAATGCTGGGTGCTGGTGGTG620.8241393061278746No Hit
CCTAGAGGGGAGTCCCTTGATGGGTACAGTGGCTCACACCTGTAATCCCAGC620.8241393061278746No Hit
CCTAGAGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCCTAGAGGGAAG620.8241393061278746No Hit
CCTAGAGGGATTGTGCACATGGGAAAGTTAAAGGATAAATTGTGAAGCCTGG610.8108467366741992No Hit
CCTAGAGGGATGGCTGTCAGCCAAAAGGAAGGCTCTGGAGCTGCCAAGAAAG590.7842615977668483No Hit
CCTAGAGGGCTGCGGACTAGCGGTGGCTTCGCGTGGGTGGAGGAGGCGCGGC570.7576764588594975No Hit
CCTAGAGGGACCCCAGCCCCAGGTCTACTCTCTGGTCACAGCTTCACAGCCA550.7310913199521467No Hit
CCTAGAGGGGAGACCCGCAGCGCGCTCCATCCTTTGCCCTTCAGTGCTGCCT540.7177987504984713No Hit
CCTAGACGGGGGCGCTCGGGTTGCCTTGCTGGTGTTGGGGGGATAGTGTGTG530.704506181044796No Hit
CCTAGAGGGGCTCTTCCGGGGACGGTGCTCAGAGGCACTCAGGATGGTCCAG530.704506181044796No Hit
CCTAGAGGGGGAGAAAGGGCAGACCCGCAGCGCGCTCCATCCTTTGCCCTTC530.704506181044796No Hit
CCTAGAGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGG510.6779210421374452No Hit
CCTAGAGGGGGTTGCACGGAGTTGTGAAGAGTACTGCCTTGTCCTTTGGCGT500.6646284726837698No Hit
CCTAGAGGGCCGTATGTCACTGGTTCGAGTCCAGTCAGAGGAGCCAAATTTT470.6247507643227436No Hit
CCTAGAGGGGAGGAAAATACCAATAGTAATTAGCATCTTAATTACCTAGGAT470.6247507643227436No Hit
CCTAGAGGGGCTCCTAGAGGGAGCTTTCCATTACACAGCAGGCTGCTGTGTA460.6114581948690682No Hit
CCTAGAGGGGCTAGGTGTGGTACTCACACTTACTGAGGATTTGTGTTATTTC460.6114581948690682No Hit
CCTAGAGGGATCCGCTGCAAGGGATGGACCCTGAGAGAACTGATACAGAAAG460.6114581948690682No Hit
CCTAGAGGGGAGTTTAGTCTTGACCTTCAGGCCTGTTCTCAGTCCCTGGACT450.5981656254153928No Hit
CCTAGAGGGATTTCAGTGGCTGCTACTCGGCGCTTCAGTCGCGGTCGCTTCA450.5981656254153928No Hit
CCTAGAGGGACTTCCGGTTGGCGTCCCACGCGTGGTGCGCGGTGTTGTCTCT440.5848730559617173No Hit
CCTAGAGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT430.571580486508042No Hit
CCTAGAGGGGATCCGAATTCAGTCTTCCTGAGGCTCTGGCTCACCTCCAGGC430.571580486508042No Hit
CCTAGACCTTTAGCAGCAAGGTCCATATCTGACTTTTTGTTAACGTATTTAG430.571580486508042No Hit
CCTAGAGGGTTATGGCCAGATCATGCGCCACCTGGATAAGGTCGAGACAAAA420.5582879170543665No Hit
CCTAGAGGGCGATGGATAAATAAATCCCTGCACACCTCAAATGTGGGAGCGG420.5582879170543665No Hit
CCTAGAGGGGATAGGGGGAAAACCCAGCAGGCACTGACTGACCTGGCATATC410.5449953476006912No Hit
CCTAGAGGGGAATGCGAATCGGGAAGAGGGGATGAGCTGCGCTGCCGTGCGT400.5317027781470158No Hit
CCTAGAGGGACACGAAGGGTGCCCTTCCCTTGGACACAGTCACTTTCTACAA380.505117639239665No Hit
CCTAGAGGGATGAGTGTTCTTTCTGCATGTATACCTGCATGTCAGAAGAGAG370.49182506978598967No Hit
CCTAGAGGGGAGAGCATGAGGAGGTTGACTGAGTTCTGGCCTGTTTGAAAGA370.49182506978598967No Hit
CCTAGACACCCACCTGGCCCAAGGCTAGCTCCTATCAGAGTTATAGTCAGCG360.4785325003323142No Hit
CCTAGAGGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAG360.4785325003323142No Hit
CCTAGAGGGGAGATCAGAGGAATTCATGGAACCAAAGAAACAGACACTGCAA350.46523993087863885No Hit
CCTAGAGGGAGGTTGACAGTAAACAGCTTGAGATTTATTGCAGAACAAAATC350.46523993087863885No Hit
CCTAGAGGGGTCTGACTCGGGGGCGGACCGGCACATCGACATCCGGGAGTCT350.46523993087863885No Hit
CCTAGAGGGGGCCCTCTCCCGTGGCCTCCCTGGGGTGGATCCTGCCTTGCGA350.46523993087863885No Hit
CCTAGAGGGGGCCTTTGAGAGCCATAATAGACATACTAAGTACTAATAATAA340.45194736142496345No Hit
CCTAGAGGGGTACCATGTTGAATTGAGACAGCCTGACTGGGGGACTCAAGTG340.45194736142496345No Hit
CCTAGAGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCA330.4386547919712881No Hit
CCTAGAGGGGGTAGGCAGATAAACAGCGGTGACAGGATTGTTTTGGTGCGCA330.4386547919712881No Hit
CCTAGAGGGAAGTGACATTTGGAGATGACAGTCACACAGAGTGGTGGAATCG330.4386547919712881No Hit
CCTAGAGGGGATGGGTGGATGGGACCGACCTTGACAAAGGTTTCAAGAATTG320.42536222251761263No Hit
CCTAGAGGGGCTTCCGGCGCGAAGGTCACGGACAAAATGGTGCCCCCGGTTC300.3987770836102618No Hit
CCTAGAGGGGGTCCGGCAGCCGAGGAGGCCCCGCGCAGTGCTGCCAACGCCC280.37219194470291106No Hit
CCTAGAGGGGGTGGAGGTTCCAGTGAGCCGAGATCACGCCATTGCACTCCAG280.37219194470291106No Hit
CCTAGAGGGTCTGGCAAGCCAGGAACTGGCGCCAGATCAGCTCGTAGAGGCG280.37219194470291106No Hit
CCTAGAGGGACTCGTACAGGAGCTTAGGCATGGGTCGTGGCACCTTCTTCCC280.37219194470291106No Hit
CCTAGACAAATTAGAGCCAATACCATCAGCTTTACCGTCTTTCCAGAAATTG260.34560680579556025No Hit
CCTAGAGGGGCTGAGGACAGCAGCAGGCTCTGGGCTCTGGGAATCCACTGCT260.34560680579556025No Hit
CCTAGAGGGCGGTGATGTAAGACAGTTGTGAAATCCCCGGGCTCAACCTGGG260.34560680579556025No Hit
CCTAGAGGGGGGGTGTTAGATCCCATTACAGATGGTTGTGAGCCACCATGTG260.34560680579556025No Hit
CCTAGAGGGAGGGCTCTTGAGTCCGTGCAGGCACAATTCTAGTGCACGCGAC250.3323142363418849No Hit
CCTAGAGGGGCAGTTCTGTCAGCGCAGCGCGATCACCAGCTCCCAGCGCCGC250.3323142363418849No Hit
CCTAGAGGGGCTTTTCGACGCGGCGGAAGAGAAAATTGGGAAAGGCCGCGAA240.3190216668882095No Hit
CCTAGAGGGATCTTAAATAACAATCAAGATAAATACCTCACAGACATGCTCT240.3190216668882095No Hit
CCTAGAGGGGACAATGGTGTCTTTGAAGTGGTGGCCACTAATGGAGATACTC240.3190216668882095No Hit
CCTAGAGGGGCCCACTCCACCCAGGCTCGGTCGTCGGAAACGCTTTCTGAGT240.3190216668882095No Hit
CCTAGAGGGAGCTCCTCTGACTTCAGTACCTCTGCTTTATAGTCAACCACAG230.3057290974345341No Hit
CCTAGAGGGACCAAAATAAAGATATGGAATTACGATTTTAAAGATAACTGAC230.3057290974345341No Hit
CCTAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA220.2924365279808587No Hit
CCTAGAGGGATTTGGGGGAAGTGACTGCACCGGCAATTTCTGTTTGTTCAAG220.2924365279808587No Hit
CCTAGAGGGACTTTACAGAAAAAGGAAAAAGTTTAGGAAACAACCAAGATAT220.2924365279808587No Hit
CCTAGAGGGGTAATATCAGTATCGTATGCCGTCTTCTGCTTGAAAAAAAAAA220.2924365279808587TruSeq Adapter, Index 15 (96% over 27bp)
CCTAGAGGGGAACGGCTGCAGGAACAGAAAGACAGGAAGCAGCAGGAGTATG220.2924365279808587No Hit
CCTAGAGGGGCATTCAGCCTTTGGTGATCCTCCAGGACCTAAGGTTTCGGTA210.27914395852718327No Hit
CCTAGAGGGGGTCTCAACCCTGTTATTTGGCTGTGTTGGGGCTTGTCCTGCT210.27914395852718327No Hit
CCTAGAGGGGAGTTCCGTTGTGGTCCTCTTCTTCACCACATGCCAACAAAGT210.27914395852718327No Hit
CCTAGAGGGATCGAGGGTTTCCTCCAGGCCCTATCTCTGGCAGTGGACAAAC210.27914395852718327No Hit
CCTAGAGGGAGACGTTCTCGCCCAGAGTCGCCGCGGTTTCCTGCTTCAACAG210.27914395852718327No Hit
CCTAGAGGGATAGTTCATCCTTCTCAGATCTCTGATGACATTGACGGCTGTC200.2658513890735079No Hit
CCTAGAGGGCTTTCTCTGTGATAACCCCAGCTGTGTCAAGTTGACACAAAAC200.2658513890735079No Hit
CCTAGAGGGGCCCCACTGGCTGCTCCGAAAAGCCATCTTTGCATTGTTCCTG200.2658513890735079No Hit
CCTAGAGGGAGGAGAGAGGAAGACCCTAGGCAGATGGAGGACTTCGGCCACA200.2658513890735079No Hit
CCTAGAGGGAAGGTGACATTCCGTTGTTTCAGCACCAATCCAACTTAAAAGA180.2392662501661571No Hit
CCTAGAGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCCTAGAGGGGAG180.2392662501661571No Hit
CCTAGAGGGGTGATTTCCTGGATTCTCAGGACTCGGGCTCGCTGTGCTTCTG170.22597368071248172No Hit
CCTAGAGGGGAGCAGCAGGCTCTGGGCTCTGGGAATCCACTGCTGTCCGGCC170.22597368071248172No Hit
CCTAGAGGGGCAGAGAAGGATGAATTGTAAATTATACTCTCGCTATGAATCC170.22597368071248172No Hit
CCTAGAGGGGATTTTTCAGTGGCTGCTACTCGGCGCTTCAGTCGCGGTCGCT170.22597368071248172No Hit
CCTAGAGGGAGGCGCGCGCGCGCGCGCACACACACACACACACACACACACA170.22597368071248172No Hit
CCTAGAGGGAAGGACCAATTTGAAAGGTAAAATAAAAAAAAAAAAAAAAAAA170.22597368071248172No Hit
CCTAGAGGGGCATTATTTTTATTTACTTTTTTGTAAAGTGATTTCCAGTCTT170.22597368071248172No Hit
CCTAGAGGGGGGCTCCACCCAAGAGGAAAAGAAAGAGAGTTAAACAGAGAAT170.22597368071248172No Hit
CCTAGAGGGGGTGGTGCTCCTGATGTCCCTCACCCACCCCTGAAGATCCCAG160.21268111125880632No Hit
CCTAGAGGGGATGAAACCTACCTTCCTGTATCTGATGTTAATATTTTTCTAA160.21268111125880632No Hit
CCTAGAGGGGGAAAAAAAATTACATCATTTGTTAAGTATAGCAGCTCACACC150.1993885418051309No Hit
CCTAGAGGGGATCTTGTTAAATTTTTTATATTTATTGATAAGATAATATATT150.1993885418051309No Hit
CCTAGAGGGGCTGTATCCTTGGTTATCAATAAAATATTTCCTGGCAAAAAAA150.1993885418051309No Hit
CCTAGAGGGGATTCTGTAGTAACTCAATTAACCATGGGCGATGGTGACTCGC150.1993885418051309No Hit
CCTAGAGGGGGCTGGCCTGCACTTAAGAGCCCTAGACCCATTTCCTCAAGGT150.1993885418051309No Hit
CCTAGAGGGGCGATCTCCGAGGCCGCATACATATTACCCACAATTCCCTTTC150.1993885418051309No Hit
CCTAGAGGGGCACTCGGAGTAAACGCGGCTGGCGGTAGTACGTGTGTCTTTT150.1993885418051309No Hit
CCTAGAGGGGTGCTCCTAGAGGGAGAAACATCCAAGGCCAGACTGGCACCCA150.1993885418051309No Hit
CCTAGAGGGCCCTCCCGGTCAAACGGGAGGGTAGCCCTGAAGGGCTATGAAA150.1993885418051309No Hit
CCTAGAGGGCATTTCCCTTCTTCAGAGGTGTAGTGTCCTGGCTCTTGGCAAG140.18609597235145553No Hit
CCTAGAGGGGCCGATGTGGGTGAAGAACTGCTCGATACGTTCCAGAGTCCGG140.18609597235145553No Hit
CCTAGAGGGCGAGCGACTAAAGTGCGCCGAATCCGTAAACCCCCATTTATAC130.17280340289778012No Hit
CCTAGAGGGATTCGCTCCTTGTTGCGTGCACCCAAGCAAGGTTTTATTTTCT130.17280340289778012No Hit
CCTAGAGGGGATGGAGGTGGGGGGGCCTGTGTGATGTGTGCTCCTTCCCTGA130.17280340289778012No Hit
CCTAGAGGGGGGGGACAGAGCCAATTTCAAGTCATGGCCAAAATTTTGTAAG130.17280340289778012No Hit
CCTAGAGGGATTCATGCTGATTATAATACAGAGGACTTATTTCTAAATGGAG130.17280340289778012No Hit
CCTAGAGGGGGAGCCACACGAAACGCCAACACCGCCACCGGACAACAACAAG130.17280340289778012No Hit
CCTAGAGGGTCTCTGGTTTTTAACTCTTTGCTGTGGAGTTTAAAGTTGGTTA120.15951083344410474No Hit
CCTAGAGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAAC120.15951083344410474No Hit
CCTAGAGGGAGCAGTGAGAGAAGTACTGTCACTTGTTTTATTTTAACAATTA120.15951083344410474No Hit
CCTAGAGGGGACGCTCAGAGCTTGAGGTCCTTGTTGCTTGTGGGTCGGGTCC120.15951083344410474No Hit
CCTAGAGGGGCCCCCTTCTCCTCTTTCCCTTTCCTCTGCTCATTGTTGACAG120.15951083344410474No Hit
CCTAGAGGGGATTCAGCCTTTGGTGATCCTCCAGGACCTAAGGTTTCGGTAT120.15951083344410474No Hit
CCTAGAGGGGCCTTTCCGGCGGTGACGACCTCCCTACGAGAACATGCCTCTC110.14621826399042934No Hit
CCTAGAGGGAAGCAGTGGTATCAACGCAGAGTGCAGATCGGAAGAGCTCGTA110.14621826399042934No Hit
CCTAGACGGGAGCATTTCAATATACTCCTACCTCCCGGCATTTAAAGCGTTT110.14621826399042934No Hit
CCTAGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC110.14621826399042934No Hit
CCTAGAGGGAGGGGTGAAGCTTCCTGGGGGTTTGGAACCCAAGGGGGAGCTG110.14621826399042934No Hit
CCTAGAGGGACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA110.14621826399042934No Hit
CCTAGAGGGAGGCTGCAATGAGCCGAGATCATGCCACTGCACTCCAGCCTGG100.13292569453675396No Hit
CCTAGAGGGATCCAGTGCATGCATGAGGTGCACACGTTCGTGTCCACGTGCC100.13292569453675396No Hit
CCTAGAGGGGAGCATCCACTTCTGTGTTTGCTGGGCCCCGGCATAGTGAGAG100.13292569453675396No Hit
CCTAGAGGGGCTCTTTCCAGCCAGCGCCGAGCGATGGGCATCTCTCGGGACA100.13292569453675396No Hit
CCTAGAGGGATGTATGTTTTTCCATAATATTTCCAGTTATGTGTATTTAAGA100.13292569453675396No Hit
CCTAGAGGGCCACGTGTCATTGCTTTCCTAGAGTGACTCACATGCAGAGCAG90.11963312508307855No Hit
CCTAGAGGGGAAGAACAGAGTGGAAGCCTCCATCACCAAGGCAAACTCATTC90.11963312508307855No Hit
CCTAGAGGGGAAGGAATCAAATTTAGAACCAAAGCCTAATTTACCACCAGCA90.11963312508307855No Hit
CCTAGAGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACAG90.11963312508307855No Hit
CCTAGAGGGGAGTCGTCGTCGCCGCTGCCACTGCCCGTGTGTGCCGCGTCAG80.10634055562940316No Hit
CCTAGAGGGAGTAATGAAGTCCAACCCCAAGTTTTGATTTTTCTCGAAAAGT80.10634055562940316No Hit
CCTAGAGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA80.10634055562940316No Hit
CCTAGAGGGGATAACTGAGGCGGAAGCATAGCTTGTGGAGCTGGGCCGCGTT80.10634055562940316No Hit
CCTAGAGGGGCGACGAAAAAATGCCTTGCAAAAAAACAAGATGATACGAAAA80.10634055562940316No Hit
CCTAGAGGGAGCTGTAGCGGCTGGGGTCCGACCTTCCATTGTGGTGCCGTGG80.10634055562940316No Hit
CCTAGAGGGGGCGAGCTTGGTAGAAAGACGAAACAAAAAAAAAAAAAAAAAA80.10634055562940316No Hit
CCTAGAGGGAGATTTAGATTCACTATATTAGGACCTTGTCCCAAAGGAAAGG80.10634055562940316No Hit
CCTAGAGGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA80.10634055562940316No Hit
CCTAGAGGGGGTTGCACGGAGTTGTGAAGAGTACTGCCTTGTCCTTTGTCGT80.10634055562940316No Hit
CCTAGAGGGGATTTCAGTGGCTGCTACTCGGCGCTTCAGTCGCGGTCGCTTC80.10634055562940316No Hit
CCTAGAGGGGCTTCCTAGATTGCAGAGCTTTCATATCCACGATGCGTTTTCT80.10634055562940316No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGCCCG205.917033E-446.00000434
TGAGACG358.503048E-846.00000443
AGCCCGT205.917033E-446.00000435
ACAAATT205.917033E-446.00000434
ATCCTGC205.917033E-446.00000439
TGCACTG205.917033E-446.00000422
AGGGGGC358.503048E-846.0000046
AGGGGAG850.046.0000046
AACGCAG358.503048E-846.00000423
GTTGACG205.917033E-446.00000440
ATCTCTA205.917033E-446.00000411
GGGCATC205.917033E-446.0000047
GGGTCCT358.503048E-846.00000429
AACAAAT205.917033E-446.00000433
CCGTGGC205.917033E-446.00000419
GCCTCCC205.917033E-446.00000424
GGCATCT205.917033E-446.0000048
GTGCACT205.917033E-446.00000421
AAATTTA205.917033E-446.00000436
CTCCCTG205.917033E-446.00000426
Produced by FastQC (version 0.11.5)