FastQCFastQC Report
Fri 17 Jun 2016
SRR1527597_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527597_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1412587
Sequences flagged as poor quality0
Sequence length50
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC221261.5663460020515552No Hit
CTTCTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG83690.592459083936069No Hit
CTTCTGGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC81770.5788670007581833No Hit
CTTCTGGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT64480.45646746005732747No Hit
CTTCTGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCC58060.41101893193127226No Hit
CTTCTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT42930.3039104848055376No Hit
CTTCTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTC42830.3032025638066894No Hit
CTTCTGGGGGCTCTCAGCTCCTGAAGGCAAATCCCAATTCCAGAGCAAGG34450.2438787841032092No Hit
CTTCTGGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCA33780.2391357134109262No Hit
CTTCTGGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTG33050.23396789011933425No Hit
CTTCTGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAA32960.23333076122037086No Hit
CTTCTGGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAG32320.22880006682774232No Hit
CTTCTGGGGGGAGTGGCCTTGTTCTCGATACTTCGTTGTGGTTGTGAACT29720.21039412085768877No Hit
CTTCTGGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTA29120.2061465948645995No Hit
CTTCTGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAG26590.18823619359373972No Hit
CTTCTGGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCG26450.1872451041953522No Hit
CTTCTGGGGGGGCTGGTGAGATGGCTCAGCGGGTAAGAGCACTGACTGCT26300.1861832226970799No Hit
CTTCTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGT25930.1835639150013415No Hit
CTTCTGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC25920.18349312290145667No Hit
CTTCTGGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCG25340.17938718110813706No Hit
CTTCTGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGA24500.17344064471781206No Hit
CTTCTGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT21810.15439756984879516No Hit
CTTCTGGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCG18400.1302574637880711No Hit
CTTCTGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGA18160.12855845339083538No Hit
CTTCTGGGGGGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTAC18130.12834607709118093No Hit
CTTCTGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCC17820.1261515219947515No Hit
CTTCTGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTA17450.1235322142990131No Hit
CTTCTGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCT16880.11949706460557827No Hit
CTTCTGGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTT16810.11900151990638452No Hit
CTTCTGGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGC16100.11397528081456222No Hit
CTTCTGGGGGAGTCCGGAGTAGGCGAGCGAGCGAGGCAGAGGTTTTTGGT15660.11086042841963008No Hit
CTTCTGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGC15550.11008171532089706No Hit
CTTCTGGGGCTCTCAGCTCCTGAAGGCAAATCCCAATTCCAGAGCAAGGA15000.10618814982723188No Hit
CTTCTGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGC14490.102577752733106No Hit
CTTCTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGC14180.10038319763667654No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGTCC207.8570953E-444.017
GCGAATA207.8570953E-444.028
GACGTTA207.8570953E-444.027
CTATACG207.8570953E-444.013
TCGATTC302.5281315E-644.020
ATCCGAT207.8570953E-444.026
CCGTAAA302.5281315E-644.023
CGCTATA207.8570953E-444.012
ATAAGCT254.4433582E-544.023
CGAATAT207.8570953E-444.029
AGTTACG254.4433582E-544.023
ATAACGC207.8570953E-444.036
ATACGTA302.5281315E-644.010
TATACGA207.8570953E-444.014
TTTTACG408.314601E-944.031
ATATTGC408.314601E-944.024
TACGTAA207.8570953E-444.011
ACGGTTA302.5281315E-644.037
ACGCGTA254.4433582E-544.020
TTCTGGG1422850.042.9454962
Produced by FastQC (version 0.11.5)