##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527597_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1412587
Sequences flagged as poor quality	0
Sequence length	50
%GC	59
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.498454962420013	33.0	31.0	34.0	30.0	34.0
2	31.813101777094083	34.0	31.0	34.0	30.0	34.0
3	31.2405635900656	31.0	31.0	34.0	30.0	34.0
4	35.690089884729225	37.0	35.0	37.0	35.0	37.0
5	35.92883978119578	37.0	35.0	37.0	35.0	37.0
6	35.91947257053902	37.0	36.0	37.0	35.0	37.0
7	36.401190156783265	37.0	37.0	37.0	35.0	37.0
8	36.489577633094456	37.0	37.0	37.0	35.0	37.0
9	38.29020867387283	39.0	39.0	39.0	37.0	39.0
10	37.291397981150894	39.0	37.0	39.0	34.0	39.0
11	35.9854302779227	37.0	35.0	39.0	32.0	39.0
12	35.98488588667459	37.0	35.0	39.0	32.0	39.0
13	36.00080419825469	38.0	35.0	39.0	32.0	39.0
14	36.77773121230763	39.0	35.0	40.0	33.0	40.0
15	36.85935945892182	39.0	35.0	40.0	33.0	40.0
16	36.97674975063483	39.0	35.0	40.0	33.0	40.0
17	36.875093711042226	38.0	35.0	40.0	33.0	40.0
18	36.745801143575584	38.0	35.0	40.0	33.0	40.0
19	36.67004439372584	38.0	35.0	40.0	32.0	40.0
20	36.49781783352105	38.0	35.0	40.0	32.0	40.0
21	36.380483467566954	38.0	35.0	40.0	32.0	40.0
22	36.21658984543961	38.0	35.0	40.0	32.0	40.0
23	36.04285045806028	38.0	35.0	40.0	31.0	40.0
24	35.895902340882365	37.0	34.0	40.0	31.0	40.0
25	35.66778966534451	37.0	34.0	39.0	31.0	40.0
26	35.23441104866461	36.0	34.0	39.0	30.0	40.0
27	35.045981592638185	36.0	34.0	38.0	30.0	40.0
28	34.869393531159496	36.0	34.0	38.0	30.0	40.0
29	34.51012857969102	35.0	33.0	38.0	29.0	40.0
30	34.195963859217166	35.0	33.0	38.0	29.0	39.0
31	33.97073454590762	35.0	33.0	38.0	27.0	39.0
32	33.885169550618826	35.0	33.0	38.0	27.0	39.0
33	33.25521472305777	35.0	32.0	38.0	25.0	39.0
34	32.674551726725504	34.0	31.0	37.0	23.0	39.0
35	32.59222830169045	34.0	31.0	37.0	24.0	38.0
36	32.35725303998975	34.0	31.0	36.0	24.0	38.0
37	32.165060983854445	34.0	31.0	36.0	24.0	38.0
38	31.624717628011584	33.0	30.0	36.0	23.0	38.0
39	30.847413999987257	33.0	29.0	35.0	21.0	38.0
40	30.569239275173846	32.0	29.0	35.0	21.0	38.0
41	30.282369156731587	32.0	28.0	35.0	21.0	37.0
42	29.8942224443521	32.0	27.0	34.0	21.0	37.0
43	29.27626900148451	31.0	27.0	34.0	19.0	37.0
44	28.795594182871568	31.0	26.0	34.0	18.0	36.0
45	28.447213516760385	30.0	24.0	33.0	20.0	36.0
46	28.325406505935565	30.0	24.0	33.0	20.0	35.0
47	27.889186294366294	30.0	24.0	33.0	18.0	35.0
48	27.368738350275063	29.0	23.0	33.0	18.0	35.0
49	26.798483208467868	29.0	23.0	32.0	17.0	34.0
50	30.363489116068603	32.0	28.0	34.0	22.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	7.0
11	16.0
12	46.0
13	76.0
14	184.0
15	320.0
16	565.0
17	866.0
18	1463.0
19	2125.0
20	3128.0
21	4311.0
22	5916.0
23	7776.0
24	10283.0
25	13477.0
26	17437.0
27	22648.0
28	29319.0
29	39117.0
30	55605.0
31	86759.0
32	144920.0
33	179874.0
34	174028.0
35	291181.0
36	278085.0
37	42143.0
38	910.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	0.0	100.0
2	0.0	0.0	100.0	0.0
3	0.0	0.0	100.0	0.0
4	0.0	0.0	0.0	100.0
5	0.0	0.0	100.0	0.0
6	100.0	0.0	0.0	0.0
7	99.14936212778399	0.5013496513843041	0.21775649924571017	0.13153172158599788
8	98.86555659934574	0.6929838657725153	0.2852921625358296	0.15616737234591568
9	94.70659152321238	2.5547453006434298	1.5944504657058292	1.144212710438366
10	64.31157868506506	22.20649064447004	5.264596092134502	8.217334578330398
11	39.26745750881185	23.733547031085518	16.657451894998328	20.34154356510431
12	33.52664296075215	15.958096740236178	25.589786682165418	24.925473616846254
13	25.672825815330313	15.548847610802024	28.223960718879614	30.554365854988045
14	24.881724099117434	16.053028946181723	31.393960159622026	27.671286795078814
15	28.868876748830335	16.038799734104874	27.738539289969395	27.353784227095396
16	34.47497393080921	16.615472179766627	23.13669883695659	25.772855052467563
17	33.30124091471888	17.562104139426456	23.467439527618474	25.669215418236185
18	32.814757604310394	18.006253774103826	22.394231293364587	26.784757328221197
19	31.174858610478505	21.707193963982398	21.17703192794497	25.94091549759413
20	33.94651090516903	20.513285199424885	20.32264207443506	25.217561820971028
21	33.24489040321056	20.672355047866077	22.1031341786382	23.979620370285158
22	31.88398307502476	17.255291178525642	25.796358029629324	25.064367716820275
23	33.25303149469732	17.842157686570808	23.13875180785325	25.76605901087862
24	33.885346530868546	17.834653723983017	21.975779190945406	26.304220554203035
25	30.45369948895183	17.980202281346212	21.05180070324872	30.514297526453237
26	26.51801269585519	18.549653932819712	25.025290477683853	29.90704289364124
27	28.782651971170626	17.327428328308276	22.01103365668805	31.878886043833056
28	29.802695338411016	21.877378172105505	19.298705141701006	29.021221347782472
29	31.947342004421674	20.097240028401792	20.301404444469615	27.654013522706922
30	30.984427861788337	18.510010356884212	22.612483337309488	27.89307844401796
31	34.2269184128128	18.99713079619167	20.39442526371827	26.381525527277255
32	36.688147349508384	18.463924699859195	20.05363209487274	24.794295855759678
33	35.8937891967008	17.992307730426514	21.685885541917063	24.428017530955614
34	29.981020638020876	21.146024988195418	25.850938738640522	23.02201563514318
35	29.507492281891313	25.21048261098254	23.99745997945613	21.284565127670014
36	30.548985655396798	26.38464037967219	19.93526770386532	23.13110626106569
37	32.35807776795341	24.581494803505908	18.498825205102413	24.56160222343827
38	33.45323155317159	24.172316466171644	19.551220561990164	22.823231418666605
39	33.8389069133441	21.66585137764966	18.552060864215797	25.943180844790444
40	29.75179581859383	21.04004921466784	19.888544917941335	29.319610048796996
41	28.32809589781019	22.586077884052454	20.175748467174056	28.910077750963303
42	26.72791127201369	19.660594356312213	21.696504356899787	31.914990014774315
43	26.892361320046128	17.118804009947706	25.19044844671514	30.798386223291025
44	27.488360008976436	17.93411662432119	23.921287679980065	30.656235686722304
45	30.144408804555045	18.916923347022166	23.033413163224637	27.905254685198148
46	28.6815608525351	21.83100934668095	21.26148690310756	28.225942897676394
47	27.63185559544297	20.502170839742966	24.129911998340635	27.73606156647343
48	29.436417013606945	19.14706846374772	25.436309409615127	25.98020511303021
49	30.302912316197162	19.01581991056126	24.016432262225266	26.66483551101631
50	26.921032120499483	19.96231028602132	23.162325577114895	29.954332016364305
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	1.0
6	2.0
7	3.5
8	5.0
9	20.0
10	35.0
11	112.5
12	190.0
13	213.0
14	236.0
15	557.5
16	879.0
17	990.5
18	1102.0
19	1104.5
20	1107.0
21	1420.0
22	1733.0
23	1790.5
24	1848.0
25	2132.5
26	2417.0
27	2769.0
28	3121.0
29	3584.5
30	4048.0
31	5685.0
32	7322.0
33	7193.5
34	7065.0
35	8905.0
36	10745.0
37	11019.0
38	11293.0
39	13123.0
40	14953.0
41	16992.5
42	19032.0
43	20105.0
44	21178.0
45	24672.0
46	28166.0
47	32710.5
48	37255.0
49	40421.5
50	43588.0
51	51242.5
52	58897.0
53	64848.0
54	70799.0
55	82457.5
56	94116.0
57	112535.5
58	130955.0
59	137622.5
60	144290.0
61	155537.5
62	166785.0
63	162595.0
64	158405.0
65	135871.5
66	113338.0
67	96322.5
68	79307.0
69	68216.5
70	57126.0
71	50059.5
72	42993.0
73	35835.5
74	28678.0
75	29876.5
76	31075.0
77	21697.0
78	12319.0
79	8301.5
80	4284.0
81	2656.5
82	1029.0
83	809.0
84	589.0
85	331.0
86	73.0
87	138.5
88	204.0
89	104.0
90	4.0
91	2.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1412587.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	11.88207584219315
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.06065141589598	8.56230125361519
2	9.254014549103426	2.199138054344115
3	3.12098004751052	1.112511648794747
4	1.382633582368706	0.6571422835067269
5	0.7976445798929612	0.47388366967012296
6	0.5507919074738489	0.39267307307223026
7	0.4414029036000071	0.36713479442776886
8	0.3849659099642935	0.36593553110837074
9	0.3249105393671715	0.34745505036197527
>10	8.378488376959176	26.727642005073584
>50	2.094898009338471	16.93182199629789
>100	1.1080004139124098	25.735707945588942
>500	0.06507017112699306	5.232347644370773
>1k	0.03253508556349653	7.248528230902269
>5k	0.00241000633803678	2.0617832223877826
>10k+	6.02501584509195E-4	1.5839935964775027
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTCTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	22126	1.5663460020515552	No Hit
CTTCTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG	8369	0.592459083936069	No Hit
CTTCTGGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC	8177	0.5788670007581833	No Hit
CTTCTGGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT	6448	0.45646746005732747	No Hit
CTTCTGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCC	5806	0.41101893193127226	No Hit
CTTCTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	4293	0.3039104848055376	No Hit
CTTCTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTC	4283	0.3032025638066894	No Hit
CTTCTGGGGGCTCTCAGCTCCTGAAGGCAAATCCCAATTCCAGAGCAAGG	3445	0.2438787841032092	No Hit
CTTCTGGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCA	3378	0.2391357134109262	No Hit
CTTCTGGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTG	3305	0.23396789011933425	No Hit
CTTCTGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAA	3296	0.23333076122037086	No Hit
CTTCTGGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAG	3232	0.22880006682774232	No Hit
CTTCTGGGGGGAGTGGCCTTGTTCTCGATACTTCGTTGTGGTTGTGAACT	2972	0.21039412085768877	No Hit
CTTCTGGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTA	2912	0.2061465948645995	No Hit
CTTCTGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAG	2659	0.18823619359373972	No Hit
CTTCTGGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCG	2645	0.1872451041953522	No Hit
CTTCTGGGGGGGCTGGTGAGATGGCTCAGCGGGTAAGAGCACTGACTGCT	2630	0.1861832226970799	No Hit
CTTCTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGT	2593	0.1835639150013415	No Hit
CTTCTGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC	2592	0.18349312290145667	No Hit
CTTCTGGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCG	2534	0.17938718110813706	No Hit
CTTCTGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGA	2450	0.17344064471781206	No Hit
CTTCTGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT	2181	0.15439756984879516	No Hit
CTTCTGGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCG	1840	0.1302574637880711	No Hit
CTTCTGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGA	1816	0.12855845339083538	No Hit
CTTCTGGGGGGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTAC	1813	0.12834607709118093	No Hit
CTTCTGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCC	1782	0.1261515219947515	No Hit
CTTCTGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTA	1745	0.1235322142990131	No Hit
CTTCTGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCT	1688	0.11949706460557827	No Hit
CTTCTGGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTT	1681	0.11900151990638452	No Hit
CTTCTGGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGC	1610	0.11397528081456222	No Hit
CTTCTGGGGGAGTCCGGAGTAGGCGAGCGAGCGAGGCAGAGGTTTTTGGT	1566	0.11086042841963008	No Hit
CTTCTGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGC	1555	0.11008171532089706	No Hit
CTTCTGGGGCTCTCAGCTCCTGAAGGCAAATCCCAATTCCAGAGCAAGGA	1500	0.10618814982723188	No Hit
CTTCTGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGC	1449	0.102577752733106	No Hit
CTTCTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGC	1418	0.10038319763667654	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	2.1237629965446377E-4	0.0	0.0	0.0	0.0
8	2.1237629965446377E-4	0.0	0.0	0.0	0.0
9	0.0012034656980419614	0.0	0.0	0.0	0.0
10	0.011609904381110685	0.0	0.0	0.0	0.0
11	0.02647624535692315	0.0	0.0	0.0	0.0
12	0.03482971314333206	0.0	0.0	0.0	0.0
13	0.04261684413066239	0.0	0.0	0.0	0.0
14	0.05217377761511326	0.0	0.0	0.0	0.0
15	0.06073962170117664	0.0	0.0	0.0	0.0
16	0.07001338678608822	0.0	0.0	0.0	0.0
17	0.0773049730742248	0.0	0.0	0.0	0.0
18	0.08410101466316765	0.0	0.0	0.0	0.0
19	0.09542775064473905	0.0	0.0	0.0	0.0
20	0.10243616853333636	0.0	0.0	0.0	0.0
21	0.11001092322101223	0.0	0.0	0.0	0.0
22	0.1169485490097247	0.0	0.0	7.079209988482125E-5	0.0
23	0.12402775899820684	0.0	0.0	7.079209988482125E-5	0.0
24	0.1302574637880711	0.0	0.0	7.079209988482125E-5	0.0
25	0.1382569710750559	0.0	0.0	7.079209988482125E-5	0.0
26	0.14710598356065857	0.0	0.0	7.079209988482125E-5	0.0
27	0.15368964884994693	0.0	0.0	7.079209988482125E-5	0.0
28	0.16621985052956031	0.0	0.0	7.079209988482125E-5	0.0
29	0.1741485657166603	0.0	0.0	7.079209988482125E-5	0.0
30	0.18179411250422098	0.0	0.0	7.079209988482125E-5	0.0
31	0.19071391708970845	0.0	0.0	7.079209988482125E-5	0.0
32	0.20062481107358343	0.0	0.0	7.079209988482125E-5	0.0
33	0.20777481316195037	0.0	0.0	7.079209988482125E-5	0.0
34	0.21726095454651642	0.0	0.0	7.079209988482125E-5	0.0
35	0.23035749302520836	0.0	0.0	7.079209988482125E-5	0.0
36	0.2461441312995235	0.0	0.0	7.079209988482125E-5	0.0
37	0.2564797778827074	0.0	0.0	7.079209988482125E-5	0.0
38	0.2656827508677342	0.0	0.0	7.079209988482125E-5	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGTCC	20	7.8570953E-4	44.0	17
GCGAATA	20	7.8570953E-4	44.0	28
GACGTTA	20	7.8570953E-4	44.0	27
CTATACG	20	7.8570953E-4	44.0	13
TCGATTC	30	2.5281315E-6	44.0	20
ATCCGAT	20	7.8570953E-4	44.0	26
CCGTAAA	30	2.5281315E-6	44.0	23
CGCTATA	20	7.8570953E-4	44.0	12
ATAAGCT	25	4.4433582E-5	44.0	23
CGAATAT	20	7.8570953E-4	44.0	29
AGTTACG	25	4.4433582E-5	44.0	23
ATAACGC	20	7.8570953E-4	44.0	36
ATACGTA	30	2.5281315E-6	44.0	10
TATACGA	20	7.8570953E-4	44.0	14
TTTTACG	40	8.314601E-9	44.0	31
ATATTGC	40	8.314601E-9	44.0	24
TACGTAA	20	7.8570953E-4	44.0	11
ACGGTTA	30	2.5281315E-6	44.0	37
ACGCGTA	25	4.4433582E-5	44.0	20
TTCTGGG	142285	0.0	42.945496	2
>>END_MODULE