FastQCFastQC Report
Fri 17 Jun 2016
SRR1527596_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527596_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1346143
Sequences flagged as poor quality0
Sequence length52
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC204701.5206408234489204No Hit
CTTCTGGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT79000.5868618712870772No Hit
CTTCTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT70630.5246842274557755No Hit
CTTCTGGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG61290.45530081128082234No Hit
CTTCTGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC57960.4305634691113797No Hit
CTTCTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC42450.31534539792577754No Hit
CTTCTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT40430.30033956273590545No Hit
CTTCTGGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG33910.2519048867765163No Hit
CTTCTGGGGGCTCTCAGCTCCTGAAGGCAAATCCCAATTCCAGAGCAAGGAA33180.2464819859405724No Hit
CTTCTGGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC32150.23883049571999407No Hit
CTTCTGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGA31270.23229330019173297No Hit
CTTCTGGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAG30070.22337894265319508No Hit
CTTCTGGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAA29580.21973891332495876No Hit
CTTCTGGGGGGAGTGGCCTTGTTCTCGATACTTCGTTGTGGTTGTGAACTCT29150.21654460187364938No Hit
CTTCTGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA26920.19997875411453314No Hit
CTTCTGGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCG26170.194407280652947No Hit
CTTCTGGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG26120.19403584908884122No Hit
CTTCTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC25570.18995010188367803No Hit
CTTCTGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGG25410.18876152087853965No Hit
CTTCTGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC23900.17754428764254615No Hit
CTTCTGGGGGGGCTGGTGAGATGGCTCAGCGGGTAAGAGCACTGACTGCTCT23900.17754428764254615No Hit
CTTCTGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC21900.16268702507831634No Hit
CTTCTGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCAT18150.13482965777038547No Hit
CTTCTGGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT18120.134606798831922No Hit
CTTCTGGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTAC18000.13371536307806822No Hit
CTTCTGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTACT17840.13252678207292984No Hit
CTTCTGGGGGGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTACTC17560.13044676531393767No Hit
CTTCTGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAG17260.1282181759293032No Hit
CTTCTGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTCT17230.12799531699083977No Hit
CTTCTGGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTG17040.12658387704723792No Hit
CTTCTGGGGGAGTCCGGAGTAGGCGAGCGAGCGAGGCAGAGGTTTTTGGTCG15600.11588664800099248No Hit
CTTCTGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCT14380.10682371783681228No Hit
CTTCTGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCGA14350.10660085889834883No Hit
CTTCTGGGGCTCTCAGCTCCTGAAGGCAAATCCCAATTCCAGAGCAAGGAAA14230.10570942314449504No Hit
CTTCTGGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCG13820.10266368431882794No Hit
CTTCTGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTC13560.10073224018547808No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATATTGC351.01974365E-746.00000424
CGAAATA206.3118414E-446.033
TAACGTA253.4174693E-546.028
CGATTCA206.3118414E-446.027
CGCGAAT206.3118414E-446.017
GTAATAC405.6134013E-946.043
CGAATAT206.3118414E-446.029
TTACGCA253.4174693E-546.020
ACGTATA206.3118414E-446.015
TCAAACG206.3118414E-446.028
CTTCTGG1368250.044.8384441
TTCTGGG1357800.044.83122
TCTGGGG1292350.044.7723
TGGGGGG359850.044.734475
CTGGGGG849450.044.7247054
CTGGGGA281500.043.9737134
TGGGGGC180600.043.8859335
TGGGGGA228050.043.7408455
CTGGGGC106700.043.4133074
TGGGGGT92150.043.3293535
Produced by FastQC (version 0.11.5)