##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527596_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1346143
Sequences flagged as poor quality	0
Sequence length	52
%GC	59
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.23289353359933	33.0	31.0	34.0	31.0	34.0
2	32.61660833953005	34.0	31.0	34.0	31.0	34.0
3	32.284393262825716	34.0	31.0	34.0	31.0	34.0
4	36.28811129278242	37.0	37.0	37.0	35.0	37.0
5	36.37548908251203	37.0	37.0	37.0	35.0	37.0
6	36.248692003747	37.0	37.0	37.0	35.0	37.0
7	36.57659401712894	37.0	37.0	37.0	35.0	37.0
8	36.62544098212449	37.0	37.0	37.0	35.0	37.0
9	38.500668948246954	39.0	39.0	39.0	38.0	39.0
10	37.67387565808387	39.0	37.0	39.0	35.0	39.0
11	36.52928180735628	37.0	35.0	39.0	33.0	39.0
12	36.43841998955534	39.0	35.0	39.0	33.0	39.0
13	36.56965938982708	39.0	35.0	39.0	33.0	39.0
14	37.30189883244202	40.0	35.0	40.0	33.0	40.0
15	37.43856261927596	40.0	35.0	40.0	33.0	40.0
16	37.5701838511956	40.0	35.0	40.0	34.0	40.0
17	37.42989934947476	40.0	35.0	40.0	33.0	40.0
18	37.33544504558579	40.0	35.0	40.0	33.0	40.0
19	37.19386870488499	39.0	35.0	40.0	33.0	40.0
20	37.133191644572676	39.0	35.0	40.0	33.0	40.0
21	37.11391360353246	39.0	35.0	40.0	33.0	40.0
22	37.139682039723866	39.0	35.0	40.0	33.0	40.0
23	37.04877490727211	39.0	35.0	40.0	33.0	40.0
24	37.03101304987657	39.0	35.0	40.0	33.0	40.0
25	37.0496514857634	39.0	35.0	40.0	33.0	40.0
26	36.95873543895411	39.0	35.0	40.0	33.0	40.0
27	36.92224748782262	39.0	35.0	40.0	33.0	40.0
28	36.766732063384055	39.0	35.0	40.0	33.0	40.0
29	36.72340085711548	39.0	35.0	40.0	33.0	40.0
30	36.58675341327036	38.0	35.0	40.0	32.0	40.0
31	36.53880902697559	38.0	35.0	40.0	32.0	40.0
32	36.483867612876196	38.0	35.0	40.0	32.0	40.0
33	36.28675036753153	38.0	35.0	40.0	32.0	40.0
34	35.74380953583683	38.0	35.0	40.0	30.0	40.0
35	35.63687661712017	38.0	34.0	40.0	30.0	40.0
36	35.66002423219524	37.0	34.0	40.0	30.0	40.0
37	35.56974333336057	37.0	34.0	40.0	30.0	40.0
38	35.64285220812351	37.0	34.0	40.0	30.0	40.0
39	35.626997280378085	37.0	34.0	40.0	31.0	40.0
40	35.385525906237305	36.0	34.0	40.0	30.0	40.0
41	35.27259956780223	36.0	34.0	40.0	30.0	40.0
42	35.14276343597968	35.0	34.0	40.0	30.0	40.0
43	35.12387836953429	35.0	34.0	40.0	30.0	40.0
44	35.01538172393275	35.0	34.0	40.0	30.0	40.0
45	34.88804086935786	35.0	34.0	39.0	29.0	40.0
46	34.67078311888113	35.0	34.0	39.0	29.0	40.0
47	34.55946062193987	35.0	34.0	39.0	29.0	40.0
48	34.361819658089814	35.0	33.0	39.0	28.0	40.0
49	34.21100210007406	35.0	33.0	39.0	27.0	40.0
50	34.60561916527442	35.0	34.0	39.0	29.0	40.0
51	34.71392638077827	35.0	34.0	39.0	30.0	40.0
52	34.36523757134272	35.0	34.0	38.0	29.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	3.0
11	11.0
12	25.0
13	23.0
14	56.0
15	97.0
16	193.0
17	318.0
18	528.0
19	842.0
20	1206.0
21	1939.0
22	2545.0
23	3542.0
24	4715.0
25	6166.0
26	8163.0
27	10515.0
28	13294.0
29	17122.0
30	22430.0
31	30154.0
32	43088.0
33	75383.0
34	190699.0
35	123526.0
36	129563.0
37	209975.0
38	362788.0
39	87233.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	0.0	100.0
2	0.0	0.0	100.0	0.0
3	0.0	0.0	100.0	0.0
4	0.0	0.0	0.0	100.0
5	0.0	0.0	100.0	0.0
6	100.0	0.0	0.0	0.0
7	99.19837639834698	0.48806107523494907	0.2051787960120136	0.10838373040605641
8	98.92492848085233	0.6592167399748764	0.27679080157160124	0.13906397760119096
9	94.88479307176132	2.4814599934776616	1.546418173997859	1.087328760763158
10	64.64870374098443	22.003308712373055	5.224259235460125	8.123728311182393
11	39.28587081758773	23.662122077669313	16.542298997951928	20.509708106791035
12	33.356188755577975	15.968065799844444	25.694224164891843	24.98152127968574
13	25.69518988695852	15.526433670122714	28.08943774918415	30.68893869373462
14	24.905080663792777	16.00780897720376	31.48424795879784	27.602862400205623
15	28.864318278221557	15.934859818013392	27.739400643170896	27.461421260594154
16	34.45102043393607	16.52632744069538	23.17012382785484	25.85252829751371
17	33.25931940365919	17.455129209898203	23.52610383889379	25.759447547548813
18	32.91604235211267	17.873509723706917	22.286562423160095	26.923885501020322
19	31.225880162805886	21.627419969498042	21.211193758761144	25.935506108934938
20	34.1118291295947	20.262408971409425	20.33974102305624	25.28602087593963
21	33.39214333098341	20.493142259031917	22.12276110338946	23.991953306595214
22	32.061081177854064	17.081543342720646	25.749270322692315	25.10810515673298
23	33.3991262443886	17.743360103644264	23.006099649145746	25.851414002821393
24	33.91578755005969	17.63586780899206	21.907033650956844	26.541310989991405
25	30.55923479154889	17.9240244164253	21.03825522251351	30.478485569512305
26	26.66574056396683	18.329256252864663	25.09911651288162	29.905886670286886
27	28.875758370396014	17.22142446976287	21.921370909331326	31.981446250509787
28	29.841777582322234	21.797832771109757	19.256126577934143	29.104263068633866
29	32.029955212782	19.854502827708497	20.28075769067625	27.83478426883325
30	30.876288774669558	18.375610912065063	22.677605573850624	28.07049473941476
31	34.33305376917608	18.816054460781658	20.382529939241227	26.468361830801037
32	36.85997698610029	18.309718952592704	19.952635046945236	24.87766901436177
33	35.90762645573316	17.816383549147453	21.630242849385244	24.645747145734145
34	30.11485406825278	20.982466201584824	25.82979668579044	23.07288304437196
35	29.723662344936606	24.94155524338796	23.870718044071097	21.464064367604333
36	30.661527044303615	26.195582490121776	19.93406346873995	23.20882699683466
37	32.52789636762216	24.400825172362815	18.433108518188632	24.638169941826387
38	33.49666417312277	24.112965710180863	19.590712130880597	22.79965798581577
39	33.85316418835146	21.486127402512214	18.61392140359531	26.046787005541017
40	29.892292275040617	20.92199714294841	19.869805808149653	29.315904773861323
41	28.40054882727912	22.429415002715164	20.344941064953723	28.82509510505199
42	27.028777774723782	19.410939253853417	21.795975613289226	31.76430735813357
43	27.044897904605975	16.96082808438628	25.203563068708153	30.790710942299594
44	27.593056606913237	17.746108697218645	23.901249718640592	30.759584977227533
45	30.37567331256783	18.642595920344274	22.982996605858368	27.998734161229528
46	28.904952891334723	21.610482690174816	21.20398798641749	28.28057643207297
47	27.82839564593063	20.24866600353751	24.17484620876088	27.748092141770968
48	29.496494800329536	18.95860989508544	25.53220571662892	26.012689587956107
49	30.460359709183944	18.624915777892838	24.121137204591193	26.79358730833203
50	26.943274228666642	19.998469701955884	23.137214991275073	29.9210410781024
51	25.699498493102148	20.329563798199747	24.821731420807446	29.14920628789066
52	26.945057100174353	20.3525925551743	24.200029268807253	28.502321075844094
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	2.5
8	5.0
9	19.5
10	34.0
11	123.0
12	212.0
13	236.5
14	683.0
15	1105.0
16	1045.0
17	985.0
18	963.5
19	942.0
20	1155.0
21	1368.0
22	1283.0
23	1198.0
24	1364.5
25	1531.0
26	1841.5
27	2152.0
28	2626.0
29	3100.0
30	4077.5
31	5055.0
32	5517.5
33	5980.0
34	7175.0
35	8370.0
36	9068.5
37	9767.0
38	10497.5
39	13607.5
40	15987.0
41	15968.5
42	15950.0
43	17795.5
44	19641.0
45	23434.5
46	27228.0
47	31212.5
48	35197.0
49	36963.0
50	38729.0
51	47114.0
52	55499.0
53	61931.5
54	68364.0
55	73188.0
56	78012.0
57	94472.0
58	110932.0
59	125886.0
60	140840.0
61	143265.0
62	145690.0
63	150709.0
64	130571.5
65	105415.0
66	94709.0
67	84003.0
68	71485.5
69	58968.0
70	51295.5
71	43623.0
72	37757.0
73	31891.0
74	33760.5
75	35630.0
76	25634.0
77	15638.0
78	11001.0
79	6364.0
80	4344.0
81	2324.0
82	1465.5
83	607.0
84	422.5
85	238.0
86	265.0
87	292.0
88	168.5
89	28.5
90	12.0
91	6.5
92	1.0
93	1.5
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1346143.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	10.118583546814502
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.40258688439562	6.92137290207995
2	9.646237198762627	1.9521251401613908
3	3.3462304981038478	1.0157733858588747
4	1.5298876937146384	0.619211857843797
5	0.898301873630312	0.45447712792941575
6	0.6274381254331335	0.3809271055591103
7	0.5012098994162212	0.3550073969213468
8	0.3757057770405186	0.3041288235204279
9	0.3910996980477627	0.35616374728471895
>10	10.341283490007076	27.954306963465992
>50	2.445021050468801	16.765400952872962
>100	1.3720103063323421	26.528519816260875
>500	0.07880037722896437	5.340519017390409
>1k	0.04050486679993495	7.523017531090304
>5k	0.002945808494540724	2.003654057510337
>10k+	7.36452123635181E-4	1.5253941742500965
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTCTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	20470	1.5206408234489204	No Hit
CTTCTGGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT	7900	0.5868618712870772	No Hit
CTTCTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	7063	0.5246842274557755	No Hit
CTTCTGGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG	6129	0.45530081128082234	No Hit
CTTCTGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC	5796	0.4305634691113797	No Hit
CTTCTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC	4245	0.31534539792577754	No Hit
CTTCTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT	4043	0.30033956273590545	No Hit
CTTCTGGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG	3391	0.2519048867765163	No Hit
CTTCTGGGGGCTCTCAGCTCCTGAAGGCAAATCCCAATTCCAGAGCAAGGAA	3318	0.2464819859405724	No Hit
CTTCTGGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC	3215	0.23883049571999407	No Hit
CTTCTGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGA	3127	0.23229330019173297	No Hit
CTTCTGGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAG	3007	0.22337894265319508	No Hit
CTTCTGGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAA	2958	0.21973891332495876	No Hit
CTTCTGGGGGGAGTGGCCTTGTTCTCGATACTTCGTTGTGGTTGTGAACTCT	2915	0.21654460187364938	No Hit
CTTCTGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA	2692	0.19997875411453314	No Hit
CTTCTGGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCG	2617	0.194407280652947	No Hit
CTTCTGGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG	2612	0.19403584908884122	No Hit
CTTCTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC	2557	0.18995010188367803	No Hit
CTTCTGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGG	2541	0.18876152087853965	No Hit
CTTCTGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC	2390	0.17754428764254615	No Hit
CTTCTGGGGGGGCTGGTGAGATGGCTCAGCGGGTAAGAGCACTGACTGCTCT	2390	0.17754428764254615	No Hit
CTTCTGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	2190	0.16268702507831634	No Hit
CTTCTGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCAT	1815	0.13482965777038547	No Hit
CTTCTGGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT	1812	0.134606798831922	No Hit
CTTCTGGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTAC	1800	0.13371536307806822	No Hit
CTTCTGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTACT	1784	0.13252678207292984	No Hit
CTTCTGGGGGGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTACTC	1756	0.13044676531393767	No Hit
CTTCTGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAG	1726	0.1282181759293032	No Hit
CTTCTGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTCT	1723	0.12799531699083977	No Hit
CTTCTGGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTG	1704	0.12658387704723792	No Hit
CTTCTGGGGGAGTCCGGAGTAGGCGAGCGAGCGAGGCAGAGGTTTTTGGTCG	1560	0.11588664800099248	No Hit
CTTCTGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCT	1438	0.10682371783681228	No Hit
CTTCTGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCGA	1435	0.10660085889834883	No Hit
CTTCTGGGGCTCTCAGCTCCTGAAGGCAAATCCCAATTCCAGAGCAAGGAAA	1423	0.10570942314449504	No Hit
CTTCTGGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCG	1382	0.10266368431882794	No Hit
CTTCTGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTC	1356	0.10073224018547808	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	2.9714525128459607E-4	0.0	0.0	0.0	0.0
8	6.685768153903411E-4	0.0	0.0	0.0	0.0
9	0.0022285893846344705	0.0	0.0	0.0	0.0
10	0.012405814241131886	0.0	0.0	0.0	0.0
11	0.02867451674896352	0.0	0.0	0.0	0.0
12	0.03937174579520898	0.0	0.0	0.0	0.0
13	0.04739466757989307	0.0	0.0	0.0	0.0
14	0.05816618293895968	0.0	0.0	0.0	0.0
15	0.06670910891339181	0.0	0.0	0.0	0.0
16	0.07614347064167774	0.0	0.0	0.0	0.0
17	0.08528068711867907	0.0	0.0	0.0	0.0
18	0.09218931421104593	0.0	0.0	0.0	0.0
19	0.10340654744703943	0.0	0.0	0.0	0.0
20	0.1127666228625042	0.0	0.0	0.0	0.0
21	0.12012096783179796	0.0	0.0	0.0	0.0
22	0.12591530023184758	0.0	0.0	0.0	0.0
23	0.13319535888832018	0.0	0.0	0.0	0.0
24	0.13921255022683326	0.0	0.0	0.0	0.0
25	0.14508116893970402	0.0	0.0	0.0	0.0
26	0.15132121921668054	0.0	0.0	0.0	0.0
27	0.1591212820629012	0.0	0.0	0.0	7.428631282114902E-5
28	0.17174995524249653	0.0	0.0	0.0	7.428631282114902E-5
29	0.1783614370835788	0.0	0.0	0.0	7.428631282114902E-5
30	0.18616149992979944	0.0	0.0	0.0	7.428631282114902E-5
31	0.19544728903244307	0.0	0.0	0.0	7.428631282114902E-5
32	0.2045845055094444	0.0	0.0	0.0	7.428631282114902E-5
33	0.21134455997616894	0.0	0.0	0.0	7.428631282114902E-5
34	0.21907033650956845	0.0	0.0	0.0	7.428631282114902E-5
35	0.2326647317558387	0.0	0.0	0.0	7.428631282114902E-5
36	0.24781913957135313	0.0	0.0	0.0	7.428631282114902E-5
37	0.2593335180586312	0.0	0.0	0.0	7.428631282114902E-5
38	0.2673564398433153	0.0	0.0	0.0	7.428631282114902E-5
39	0.27485935743825135	0.0	0.0	0.0	7.428631282114902E-5
40	0.2828079929101143	0.0	0.0	0.0	7.428631282114902E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATATTGC	35	1.01974365E-7	46.000004	24
CGAAATA	20	6.3118414E-4	46.0	33
TAACGTA	25	3.4174693E-5	46.0	28
CGATTCA	20	6.3118414E-4	46.0	27
CGCGAAT	20	6.3118414E-4	46.0	17
GTAATAC	40	5.6134013E-9	46.0	43
CGAATAT	20	6.3118414E-4	46.0	29
TTACGCA	25	3.4174693E-5	46.0	20
ACGTATA	20	6.3118414E-4	46.0	15
TCAAACG	20	6.3118414E-4	46.0	28
CTTCTGG	136825	0.0	44.838444	1
TTCTGGG	135780	0.0	44.8312	2
TCTGGGG	129235	0.0	44.772	3
TGGGGGG	35985	0.0	44.73447	5
CTGGGGG	84945	0.0	44.724705	4
CTGGGGA	28150	0.0	43.973713	4
TGGGGGC	18060	0.0	43.885933	5
TGGGGGA	22805	0.0	43.740845	5
CTGGGGC	10670	0.0	43.413307	4
TGGGGGT	9215	0.0	43.329353	5
>>END_MODULE