FastQCFastQC Report
Fri 17 Jun 2016
SRR1527595_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527595_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1657559
Sequences flagged as poor quality0
Sequence length52
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC250161.5092072137402048No Hit
CTTCTGGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT99150.5981687529674661No Hit
CTTCTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT85380.515094786972892No Hit
CTTCTGGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG79230.47799203527596906No Hit
CTTCTGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC73090.44094961325660204No Hit
CTTCTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC54330.32777113816159786No Hit
CTTCTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT48520.29271959550157794No Hit
CTTCTGGGGGCTCTCAGCTCCTGAAGGCAAATCCCAATTCCAGAGCAAGGAA42200.25459123928620336No Hit
CTTCTGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGA41330.2493425573388338No Hit
CTTCTGGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC39740.2397501386074342No Hit
CTTCTGGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAA39260.2368543140847475No Hit
CTTCTGGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG38730.23365684117428098No Hit
CTTCTGGGGGGAGTGGCCTTGTTCTCGATACTTCGTTGTGGTTGTGAACTCT37440.22587431276956055No Hit
CTTCTGGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAG36440.21984134501396332No Hit
CTTCTGGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCG32630.19685573786513783No Hit
CTTCTGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC32100.19365826495467128No Hit
CTTCTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC31070.18744430816640614No Hit
CTTCTGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA30920.18653936300306656No Hit
CTTCTGGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG30750.18551375848461502No Hit
CTTCTGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGG30500.18400551654571573No Hit
CTTCTGGGGGGGCTGGTGAGATGGCTCAGCGGGTAAGAGCACTGACTGCTCT30020.18110969202302904No Hit
CTTCTGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC24430.1473854022692405No Hit
CTTCTGGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT22740.13718968676228116No Hit
CTTCTGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAG21630.13049309255356822No Hit
CTTCTGGGGGGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTACTC21600.13031210352090034No Hit
CTTCTGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCAT21280.12838155383910918No Hit
CTTCTGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTCT21130.12747660867576963No Hit
CTTCTGGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTAC20840.12572704802664642No Hit
CTTCTGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTACT20030.12084034414461264No Hit
CTTCTGGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTG19950.12035770672416486No Hit
CTTCTGGGGGAGTCCGGAGTAGGCGAGCGAGCGAGGCAGAGGTTTTTGGTCG19620.11836682736481778No Hit
CTTCTGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCT18250.11010166153964956No Hit
CTTCTGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCGA17720.10690418862918302No Hit
CTTCTGGGGCTCTCAGCTCCTGAAGGCAAATCCCAATTCCAGAGCAAGGAAA17580.10605957314339942No Hit
CTTCTGGGGGCTCTTTTTCGTGACGCCTCCCAGGCGCTCGGCTGTGTCAAGA16740.10099188022869773No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCATA253.417814E-546.029
ATTAGCG206.312266E-446.013
CCGATAT206.312266E-446.042
TAACGAT206.312266E-446.028
CGTTAGG206.312266E-446.024
CTACGTT253.417814E-546.035
TACGACT206.312266E-446.012
TATTCGG301.8619048E-646.035
CCGGTTA453.110472E-1046.026
ATACGTA253.417814E-546.010
TATCGCG206.312266E-446.040
CCGTTAT206.312266E-446.011
TTCTGGG1672300.044.8557052
CTTCTGG1687300.044.8290751
TCTGGGG1599500.044.773433
CTGGGGG1042250.044.7553864
TGGGGGG441150.044.514115
TGGGGGC218450.044.09435
TGGGGGA282400.044.037185
CTGGGGA361000.044.0058144
Produced by FastQC (version 0.11.5)