##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527595_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1657559
Sequences flagged as poor quality	0
Sequence length	52
%GC	59
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.26598449889265	31.0	31.0	34.0	30.0	34.0
2	31.806734481246217	33.0	31.0	34.0	30.0	34.0
3	31.168876643305005	31.0	31.0	34.0	30.0	34.0
4	35.79264448505302	37.0	35.0	37.0	35.0	37.0
5	35.94762418713301	37.0	35.0	37.0	35.0	37.0
6	35.87311039908685	37.0	35.0	37.0	35.0	37.0
7	36.40893747975185	37.0	37.0	37.0	35.0	37.0
8	36.48063869823035	37.0	37.0	37.0	35.0	37.0
9	38.395429664947066	39.0	39.0	39.0	37.0	39.0
10	37.191024874529354	39.0	37.0	39.0	34.0	39.0
11	35.8395604621012	37.0	35.0	39.0	31.0	39.0
12	35.7339032879071	37.0	35.0	39.0	31.0	39.0
13	35.800245421128295	37.0	35.0	39.0	31.0	39.0
14	36.57357837639565	39.0	35.0	40.0	32.0	40.0
15	36.73711101686275	39.0	35.0	40.0	32.0	40.0
16	36.84768566307444	39.0	35.0	40.0	33.0	40.0
17	36.702185563228824	39.0	35.0	40.0	32.0	40.0
18	36.64584307406252	38.0	35.0	40.0	32.0	40.0
19	36.45256971245066	38.0	35.0	40.0	31.0	40.0
20	36.348443102176155	38.0	35.0	40.0	31.0	40.0
21	36.39376878892396	38.0	35.0	40.0	31.0	40.0
22	36.43162324840323	38.0	35.0	40.0	31.0	40.0
23	36.441692271587314	38.0	35.0	40.0	31.0	40.0
24	36.45344690596232	38.0	35.0	40.0	31.0	40.0
25	36.44129590560577	38.0	35.0	40.0	31.0	40.0
26	36.29656199266512	38.0	35.0	40.0	31.0	40.0
27	36.25027827063773	38.0	35.0	40.0	31.0	40.0
28	36.03546359435773	38.0	35.0	40.0	31.0	40.0
29	35.98918650859487	38.0	35.0	40.0	31.0	40.0
30	35.91966982774067	38.0	34.0	40.0	31.0	40.0
31	35.84374794502036	38.0	34.0	40.0	30.0	40.0
32	35.764662374009006	38.0	34.0	40.0	30.0	40.0
33	35.561960087091926	37.0	34.0	40.0	30.0	40.0
34	35.1019396594631	37.0	34.0	40.0	27.0	40.0
35	35.01957577377336	37.0	33.0	40.0	28.0	40.0
36	35.096992022606734	36.0	34.0	40.0	29.0	40.0
37	35.08698936206795	36.0	34.0	40.0	29.0	40.0
38	34.947044418931696	36.0	34.0	40.0	29.0	40.0
39	34.8227453743728	35.0	34.0	39.0	29.0	40.0
40	35.010047907796945	35.0	34.0	40.0	29.0	40.0
41	35.11585107981073	35.0	34.0	40.0	29.0	40.0
42	35.150722840031634	35.0	34.0	40.0	30.0	40.0
43	35.122223703650974	35.0	34.0	40.0	30.0	40.0
44	35.024845571107875	35.0	34.0	39.0	30.0	40.0
45	34.93994180599303	35.0	34.0	39.0	30.0	40.0
46	34.65130894284909	35.0	34.0	39.0	29.0	40.0
47	34.58737758354303	35.0	34.0	39.0	29.0	40.0
48	34.46860956382246	35.0	33.0	39.0	29.0	40.0
49	34.33007392195391	35.0	33.0	38.0	28.0	40.0
50	34.21537031261029	35.0	33.0	38.0	28.0	40.0
51	34.098219128248225	35.0	33.0	38.0	28.0	40.0
52	33.62455695393045	35.0	33.0	37.0	27.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	8.0
13	15.0
14	57.0
15	111.0
16	210.0
17	438.0
18	743.0
19	1322.0
20	1949.0
21	3001.0
22	4184.0
23	5910.0
24	8039.0
25	10917.0
26	14451.0
27	18358.0
28	23434.0
29	29892.0
30	38722.0
31	50093.0
32	69841.0
33	111603.0
34	235935.0
35	145027.0
36	178829.0
37	272050.0
38	380986.0
39	51432.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	0.0	100.0
2	0.0	0.0	100.0	0.0
3	0.0	0.0	100.0	0.0
4	0.0	0.0	0.0	100.0
5	0.0	0.0	100.0	0.0
6	100.0	0.0	0.0	0.0
7	99.26325397768646	0.4690632429976851	0.17984276879435362	0.08784001052149576
8	98.96588899701307	0.6627215079523564	0.24517980958747168	0.1262096854470942
9	94.88639620067822	2.5066981024506516	1.491228969828525	1.115676727042597
10	64.50762838607857	22.096408031327993	5.146724792300002	8.249238790293438
11	39.00265390251569	23.84391747141429	16.64550100479078	20.507927621279244
12	33.23453343138917	16.037317525349025	25.726565389225964	25.00158365403584
13	25.500570417101294	15.584965603034343	28.221619863908316	30.692844115956053
14	24.94221925132077	16.01861532530667	31.27605110888964	27.763114314482923
15	28.76072586254848	15.978073781989059	27.76510519384227	27.496095161620186
16	34.265205642755404	16.59385880080287	23.20520717512921	25.935728381312522
17	33.139152211173176	17.55213539910193	23.4207651130367	25.887947276688188
18	32.75961820966856	17.853301149461345	22.25585936910843	27.131221271761667
19	31.05029745547519	21.56846302303568	21.21185429900233	26.169385222486802
20	34.00741693055873	20.183293626350554	20.365006615149145	25.44428282794157
21	33.28424508569529	20.442107943065675	22.105517812638947	24.168129158600085
22	32.05201142161456	17.09845622388102	25.699296374970665	25.150235979533758
23	33.2616214566118	17.807691913229032	23.06156221286844	25.869124417290728
24	33.789023497806106	17.74271685050125	21.963501751672187	26.504757900020454
25	30.467874748349832	17.935892478035473	21.059823511561277	30.536409262053414
26	26.640740993231617	18.39397571971797	24.982700464960825	29.982582822089594
27	28.928502695831643	17.285719543014757	21.85545129916944	31.93032646198416
28	29.97015490851306	21.665292155513015	19.321665171496157	29.042887764477765
29	32.0070054821578	19.930753596101255	20.216716267716563	27.845524654024384
30	30.887889963494512	18.498165072857134	22.593886552454542	28.02005841119381
31	34.32354444095203	19.034194257941948	20.31879408214127	26.32346721896475
32	36.71790868379346	18.399465720375563	19.94070799289799	24.941917602932985
33	35.780626813283874	17.974624131026406	21.53878082167814	24.70596823401158
34	30.022521068631647	21.09915846132777	25.655135050999693	23.223185419040888
35	29.62024277868842	25.120191800110884	23.838789448822034	21.42077597237866
36	30.626783119032265	26.186579180590254	19.89021205278364	23.29642564759384
37	32.28946903247486	24.40739665978707	18.55758980524977	24.745544502488297
38	33.532924016581006	24.009522436305435	19.58464223596264	22.872911311150915
39	33.817559435290086	21.518268731309114	18.58588442402352	26.078287409377282
40	29.791639392624937	20.891986348600565	20.00857888014846	29.30779537862604
41	28.444115714734743	22.391058176511365	20.26111891039776	28.903707198356138
42	26.72303067341796	19.50723925965833	21.80513634808776	31.96459371883595
43	26.96024696556804	16.960844229375844	25.25931203655496	30.81959676850115
44	27.565051983066667	17.791040922223583	23.93411033936047	30.709796755349284
45	30.16025372249193	18.59113310597089	23.157184751794656	28.091428419742527
46	28.930252256480767	21.558568955916503	21.31399244310459	28.197186344498142
47	27.737594860876747	20.354690240287074	24.05977705770956	27.84793784112662
48	29.44540737313121	18.98743875783607	25.523133716507225	26.044020152525494
49	30.25672087690393	18.707267735266136	24.154675640505104	26.88133574732483
50	26.850024644673283	19.947404587106703	23.24297355327925	29.959597214940764
51	25.713775497584095	20.29255067240442	24.818422752975913	29.17525107703557
52	26.95053388748153	20.52669015099915	24.022674306012636	28.500101655506683
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	1.0
6	2.0
7	3.0
8	4.0
9	19.5
10	35.0
11	125.0
12	215.0
13	248.0
14	752.5
15	1224.0
16	1164.0
17	1104.0
18	1188.0
19	1272.0
20	1473.5
21	1675.0
22	1651.0
23	1627.0
24	1836.5
25	2046.0
26	2380.5
27	2715.0
28	3384.0
29	4053.0
30	5220.5
31	6388.0
32	6831.0
33	7274.0
34	8833.0
35	10392.0
36	11230.5
37	12069.0
38	12799.0
39	16579.5
40	19630.0
41	19892.0
42	20154.0
43	22402.5
44	24651.0
45	28927.5
46	33204.0
47	38214.0
48	43224.0
49	45525.5
50	47827.0
51	58975.5
52	70124.0
53	77186.5
54	84249.0
55	90237.0
56	96225.0
57	116089.5
58	135954.0
59	154074.5
60	172195.0
61	175454.5
62	178714.0
63	184835.0
64	160775.5
65	130595.0
66	117165.5
67	103736.0
68	87932.5
69	72129.0
70	62967.5
71	53806.0
72	46459.5
73	39113.0
74	41965.0
75	44817.0
76	31914.5
77	19012.0
78	13150.5
79	7289.0
80	4994.5
81	2700.0
82	1707.5
83	715.0
84	477.0
85	239.0
86	284.0
87	329.0
88	185.0
89	28.0
90	15.0
91	11.5
92	8.0
93	5.5
94	3.0
95	1.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1657559.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	9.734769708246093
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.68974784049583	6.784136462529673
2	9.874516570413771	1.9225228958647618
3	3.6459876845775443	1.0647855140539138
4	1.6722346657904275	0.6511527747846272
5	0.9161514055336689	0.44592614753781207
6	0.6087367793503727	0.3555547415949176
7	0.4448519537876665	0.30313719270703865
8	0.3763486530443452	0.2930933973916249
9	0.30897045579526144	0.27069806104368294
>10	7.918219696631573	21.62994427865616
>50	2.905350507397744	19.41056730499405
>100	1.5008594318121453	28.71324455630114
>500	0.08828584893012618	5.7717610097227
>1k	0.046008118456544636	8.501822185079618
>5k	0.003108656652469232	2.3675822612368798
>10k+	6.217313304938464E-4	1.5140712165014005
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTCTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	25016	1.5092072137402048	No Hit
CTTCTGGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT	9915	0.5981687529674661	No Hit
CTTCTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	8538	0.515094786972892	No Hit
CTTCTGGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG	7923	0.47799203527596906	No Hit
CTTCTGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC	7309	0.44094961325660204	No Hit
CTTCTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC	5433	0.32777113816159786	No Hit
CTTCTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT	4852	0.29271959550157794	No Hit
CTTCTGGGGGCTCTCAGCTCCTGAAGGCAAATCCCAATTCCAGAGCAAGGAA	4220	0.25459123928620336	No Hit
CTTCTGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGA	4133	0.2493425573388338	No Hit
CTTCTGGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC	3974	0.2397501386074342	No Hit
CTTCTGGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAA	3926	0.2368543140847475	No Hit
CTTCTGGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG	3873	0.23365684117428098	No Hit
CTTCTGGGGGGAGTGGCCTTGTTCTCGATACTTCGTTGTGGTTGTGAACTCT	3744	0.22587431276956055	No Hit
CTTCTGGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAG	3644	0.21984134501396332	No Hit
CTTCTGGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCG	3263	0.19685573786513783	No Hit
CTTCTGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC	3210	0.19365826495467128	No Hit
CTTCTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC	3107	0.18744430816640614	No Hit
CTTCTGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA	3092	0.18653936300306656	No Hit
CTTCTGGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG	3075	0.18551375848461502	No Hit
CTTCTGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGG	3050	0.18400551654571573	No Hit
CTTCTGGGGGGGCTGGTGAGATGGCTCAGCGGGTAAGAGCACTGACTGCTCT	3002	0.18110969202302904	No Hit
CTTCTGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	2443	0.1473854022692405	No Hit
CTTCTGGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT	2274	0.13718968676228116	No Hit
CTTCTGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAG	2163	0.13049309255356822	No Hit
CTTCTGGGGGGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTACTC	2160	0.13031210352090034	No Hit
CTTCTGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCAT	2128	0.12838155383910918	No Hit
CTTCTGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTCT	2113	0.12747660867576963	No Hit
CTTCTGGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTAC	2084	0.12572704802664642	No Hit
CTTCTGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTACT	2003	0.12084034414461264	No Hit
CTTCTGGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTG	1995	0.12035770672416486	No Hit
CTTCTGGGGGAGTCCGGAGTAGGCGAGCGAGCGAGGCAGAGGTTTTTGGTCG	1962	0.11836682736481778	No Hit
CTTCTGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCT	1825	0.11010166153964956	No Hit
CTTCTGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCGA	1772	0.10690418862918302	No Hit
CTTCTGGGGCTCTCAGCTCCTGAAGGCAAATCCCAATTCCAGAGCAAGGAAA	1758	0.10605957314339942	No Hit
CTTCTGGGGGCTCTTTTTCGTGACGCCTCCCAGGCGCTCGGCTGTGTCAAGA	1674	0.10099188022869773	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	3.0164838777986183E-4	0.0	0.0	0.0	0.0
8	4.826374204477789E-4	0.0	0.0	0.0	0.0
9	0.0015685716164552815	0.0	0.0	0.0	0.0
10	0.010678352927407108	0.0	0.0	0.0	0.0
11	0.02551945360617631	0.0	0.0	0.0	0.0
12	0.034327586529348274	0.0	0.0	0.0	0.0
13	0.04102418073806121	0.0	0.0	0.0	0.0
14	0.04965132462856526	0.0	0.0	0.0	0.0
15	0.05827846851906931	0.0	0.0	0.0	0.0
16	0.0652767111155621	0.0	0.0	0.0	0.0
17	0.07257660209983476	0.0	0.0	0.0	0.0
18	0.07909220727587978	0.0	0.0	0.0	0.0
19	0.09031352730129064	0.0	0.0	0.0	0.0
20	0.09791506667334315	0.0	0.0	0.0	0.0
21	0.10412902346160831	0.0	0.0	0.0	0.0
22	0.11022232089476151	0.0	0.0	0.0	0.0
23	0.1161346292952468	0.0	0.0	0.0	0.0
24	0.12367583898974335	0.0	0.0	0.0	0.0
25	0.1298897957780085	0.0	0.0	0.0	0.0
26	0.13767232418272893	0.0	0.0	0.0	0.0
27	0.1449118854894456	0.0	0.0	0.0	0.0
28	0.1569778210006401	0.0	0.0	0.0	0.0
29	0.16361408553179707	0.0	0.0	0.0	0.0
30	0.17055199845073388	0.0	0.0	0.0	0.0
31	0.17899815330857002	0.0	0.0	0.0	0.0
32	0.18768562687663004	0.0	0.0	0.0	0.0
33	0.194321891407787	0.0	0.0	0.0	0.0
34	0.20210441981250743	0.0	0.0	0.0	0.0
35	0.21477365209926164	0.0	0.0	0.0	0.0
36	0.22913211535758304	0.0	0.0	0.0	0.0
37	0.23830222634609086	0.0	0.0	0.0	0.0
38	0.24590376571814337	0.0	0.0	0.0	0.0
39	0.253324316057528	0.0	0.0	0.0	0.0
40	0.2615894818826962	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATCATA	25	3.417814E-5	46.0	29
ATTAGCG	20	6.312266E-4	46.0	13
CCGATAT	20	6.312266E-4	46.0	42
TAACGAT	20	6.312266E-4	46.0	28
CGTTAGG	20	6.312266E-4	46.0	24
CTACGTT	25	3.417814E-5	46.0	35
TACGACT	20	6.312266E-4	46.0	12
TATTCGG	30	1.8619048E-6	46.0	35
CCGGTTA	45	3.110472E-10	46.0	26
ATACGTA	25	3.417814E-5	46.0	10
TATCGCG	20	6.312266E-4	46.0	40
CCGTTAT	20	6.312266E-4	46.0	11
TTCTGGG	167230	0.0	44.855705	2
CTTCTGG	168730	0.0	44.829075	1
TCTGGGG	159950	0.0	44.77343	3
CTGGGGG	104225	0.0	44.755386	4
TGGGGGG	44115	0.0	44.51411	5
TGGGGGC	21845	0.0	44.0943	5
TGGGGGA	28240	0.0	44.03718	5
CTGGGGA	36100	0.0	44.005814	4
>>END_MODULE