FastQCFastQC Report
Fri 17 Jun 2016
SRR1527594_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527594_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2411012
Sequences flagged as poor quality0
Sequence length50
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGCCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC314171.303062780276498No Hit
GTTGCCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG189310.7851889579977204No Hit
GTTGCCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT144880.600909493606834No Hit
GTTGCCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT127260.5278281485119112No Hit
GTTGCCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGT91960.38141660016623724No Hit
GTTGCCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTT52440.21750202819397No Hit
GTTGCCGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCC47870.19854733199171137No Hit
GTTGCCGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGC44140.1830766499710495No Hit
GTTGCCGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT42410.17590123981133235No Hit
GTTGCCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC38760.16076236866510826No Hit
GTTGCCGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTC37600.15595111098576034No Hit
GTTGCCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTG33380.13844808735916703No Hit
GTTGCCGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC32080.13305616064955297No Hit
GTTGCCGGGGGGGCTGGCGAGATGGCTCAGTGGGTAAGAGCACCCGACTG30250.12546598689678856No Hit
GTTGCCGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCT29670.12306035805711461No Hit
GTTGCCGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCA27000.11198617012275344No Hit
GTTGCCGGGGGGGCTGGCGAGATGGCTCAGCGGGTAAGAGCACCCGATTG25280.104852236322341No Hit
GTTGCCGGGGGCAGTGGTGGCGCACGCCTTTCGTCCCAGCACTTGGGAGG25220.10460337816651265No Hit
GTTGCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGTTGCCGGGA25000.10369089826180874No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAACGC207.8582024E-444.00000417
GCGTAAC207.8582024E-444.00000433
CGTACCG254.4442997E-544.032
TATACGC254.4442997E-544.039
TGCCGGA110350.042.624383
GTTGCCG2479150.042.0423931
TTGCCGG2446600.041.9902762
CGGGATA36750.041.9047626
TGCCGGG2274950.041.7970473
CCGGGAT284900.041.7220085
CGGGATT97050.041.710466
GCCGGGA883450.041.6591764
GCCGGGG1093100.041.554664
GCCGGAT33050.041.5370674
CCGGGGG483500.041.4564635
CGGGGGG272650.041.434076
CGGGATC85650.041.3543476
TGCCGGT32850.041.3211563
CCGGGAC135250.041.2835465
CCGGGAA167600.041.1252945
Produced by FastQC (version 0.11.5)