##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527594_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2411012
Sequences flagged as poor quality	0
Sequence length	50
%GC	57
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.990630905196657	31.0	31.0	33.0	28.0	34.0
2	30.752834079631292	31.0	31.0	33.0	28.0	34.0
3	30.684694227983933	31.0	31.0	33.0	28.0	34.0
4	35.39148623067824	37.0	35.0	37.0	33.0	37.0
5	35.755133528991145	37.0	35.0	37.0	35.0	37.0
6	36.22066335630018	37.0	36.0	37.0	35.0	37.0
7	36.13095206494202	37.0	36.0	37.0	35.0	37.0
8	36.359020195668876	37.0	37.0	37.0	35.0	37.0
9	38.132450191040114	39.0	39.0	39.0	37.0	39.0
10	36.5604298112162	39.0	37.0	39.0	31.0	39.0
11	36.968378008902484	39.0	37.0	39.0	34.0	39.0
12	36.718482529327936	38.0	35.0	39.0	33.0	39.0
13	36.83012403090486	39.0	35.0	39.0	33.0	39.0
14	37.5770315535551	40.0	37.0	40.0	33.0	40.0
15	37.60312764930245	40.0	37.0	40.0	33.0	40.0
16	37.673218963655096	40.0	37.0	40.0	33.0	40.0
17	37.573836214834266	40.0	37.0	40.0	33.0	40.0
18	37.43999324764871	39.0	36.0	40.0	33.0	40.0
19	37.34942173659857	39.0	36.0	40.0	33.0	40.0
20	37.1344373234144	39.0	36.0	40.0	33.0	40.0
21	37.076869795753815	39.0	36.0	40.0	33.0	40.0
22	36.90552888164804	38.0	35.0	40.0	32.0	40.0
23	36.695105208933015	38.0	35.0	40.0	32.0	40.0
24	36.581968484603145	38.0	35.0	40.0	32.0	40.0
25	36.367964157789345	38.0	35.0	40.0	32.0	40.0
26	35.898763257918255	38.0	35.0	39.0	31.0	40.0
27	35.68821308230735	37.0	34.0	39.0	30.0	40.0
28	35.53413006654467	37.0	34.0	39.0	30.0	40.0
29	35.2096733653752	36.0	34.0	38.0	30.0	40.0
30	34.9105777988662	36.0	34.0	38.0	30.0	40.0
31	34.78704626936739	36.0	33.0	38.0	29.0	40.0
32	34.614218842544126	36.0	33.0	38.0	29.0	39.0
33	34.03798736796001	35.0	33.0	38.0	27.0	39.0
34	33.52843909528447	35.0	32.0	38.0	26.0	39.0
35	33.33114974127047	35.0	32.0	38.0	26.0	39.0
36	33.113607066244384	34.0	31.0	37.0	26.0	39.0
37	32.92949972874461	34.0	31.0	37.0	26.0	38.0
38	32.542473036218816	34.0	31.0	37.0	25.0	38.0
39	31.85616247451278	34.0	30.0	36.0	23.0	38.0
40	31.61012471111716	33.0	30.0	36.0	23.0	38.0
41	31.322136928393554	33.0	29.0	36.0	23.0	38.0
42	30.815135718942916	33.0	29.0	35.0	23.0	38.0
43	30.28301352295219	32.0	28.0	35.0	22.0	37.0
44	29.774826089625435	31.0	27.0	34.0	21.0	37.0
45	29.254428430882964	31.0	26.0	34.0	20.0	37.0
46	29.03455022206443	31.0	26.0	34.0	20.0	36.0
47	28.596709182700046	31.0	26.0	34.0	19.0	36.0
48	28.0855802459714	30.0	24.0	33.0	18.0	35.0
49	27.665904192928114	29.0	24.0	33.0	18.0	35.0
50	31.1074557073959	33.0	29.0	35.0	23.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	8.0
11	24.0
12	85.0
13	185.0
14	353.0
15	579.0
16	930.0
17	1374.0
18	1936.0
19	2654.0
20	3806.0
21	5336.0
22	7478.0
23	10089.0
24	14110.0
25	18398.0
26	24721.0
27	32499.0
28	41675.0
29	54286.0
30	72363.0
31	99505.0
32	159156.0
33	249757.0
34	324518.0
35	563466.0
36	610596.0
37	109343.0
38	1779.0
39	2.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	100.0	0.0	0.0	0.0
2	0.0	0.0	100.0	0.0
3	0.0	0.0	100.0	0.0
4	100.0	0.0	0.0	0.0
5	0.0	0.0	0.0	100.0
6	0.0	0.0	0.0	100.0
7	98.20009191161222	0.7282419166723352	0.38270236730468365	0.688963804410762
8	97.8258507216057	1.1128107201457313	0.4738674050564659	0.5874711531921035
9	92.02998574872294	4.68239063098815	1.5239658699334553	1.7636577503554525
10	46.6816838738256	37.75381458076525	6.407475367190209	9.157026178218938
11	37.02324998797186	19.61653446768411	24.38469820971443	18.975517334629608
12	31.130703621549788	16.628411637934608	26.662082146418186	25.578802594097418
13	27.912926190330033	16.736872317516465	28.303052825950264	27.047148666203235
14	26.080417683528744	18.587671898771138	31.434186142582448	23.897724275117668
15	29.336892557979805	18.147358868392192	29.762357051727655	22.753391521900348
16	34.577264650694396	19.802016746494832	24.44811556309135	21.17260303971942
17	31.859609159970997	20.749378269374024	22.671600141351433	24.719412429303546
18	31.049161099156702	21.378906450901116	22.549784074073457	25.022148375868724
19	31.629664223985614	23.55239210754654	22.48586900438488	22.332074664082967
20	32.006435471909725	24.832767319283356	22.550281790385114	20.61051541842181
21	30.84787632745088	23.457867484691075	25.307256869729393	20.386999318128655
22	29.35987046103462	21.234112480568328	26.53707239947375	22.868944658923308
23	29.240252640799795	23.050611112677995	25.837117359847234	21.872018886674972
24	29.924363711171907	21.583550807710623	23.226056112536977	25.266029368580494
25	27.24768686344158	21.19657637539755	23.62588821623451	27.92984854492636
26	25.704641868227945	22.417515964250697	24.960307124145377	26.917535043375977
27	23.28868541508711	22.125066154793092	24.370969534784564	30.215278895335235
28	25.50281790385116	25.924591001620893	23.11850791285983	25.454083181668114
29	28.669952700359847	23.6187542824341	23.29959369758425	24.411699319621803
30	28.203924327211976	22.055800634754203	24.596103213090604	25.144171824943218
31	31.497479066881457	21.583758189507144	24.207386773686736	22.711375969924664
32	32.762300643879	20.71503584386971	23.667032764664796	22.855630747586492
33	32.3030329172978	20.904665758610907	24.58706136676217	22.20523995732912
34	27.437192349104855	23.77006004117773	27.37195003591853	21.420797573798886
35	25.716670012426317	28.080573634639727	25.892032059566688	20.310724293367265
36	26.838149291666735	27.619646853686337	23.526801193855526	22.015402660791402
37	28.092726207916012	28.64730660817947	21.813329838258788	21.446637345645726
38	29.05667827451709	26.799327419357517	21.351863864634435	22.79213044149096
39	28.487290813981847	24.424432561928352	21.58450476397463	25.503771860115172
40	25.84226042840102	24.964911000028202	22.014821991761135	27.178006579809637
41	24.33828616365244	25.496679402674065	22.263058002199905	27.901976431473592
42	22.64215192624508	23.972423198225474	23.852390614397606	29.533034261131842
43	23.854588861440757	22.114821494044826	24.286399238162232	29.74419040635219
44	25.875565944922712	21.445849294818938	23.331489017889584	29.34709574236877
45	26.49696476002608	23.69992351759344	22.59694269460293	27.20616902777755
46	25.233263044729764	24.812402426864736	23.33795932994112	26.616375198464382
47	25.007507221034153	23.81958281418757	25.04496037348632	26.127949591291955
48	26.54623867488009	22.534852584723758	25.8561135324088	25.062795207987353
49	27.0836478623914	22.174962215036672	25.28220514870934	25.459184773862592
50	25.150268849761016	22.048708177313095	26.540929700889087	26.260093272036805
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	6.0
8	12.0
9	55.0
10	98.0
11	170.0
12	242.0
13	594.0
14	946.0
15	2029.5
16	3113.0
17	3386.5
18	3660.0
19	3640.5
20	3621.0
21	3711.0
22	3801.0
23	4288.5
24	4776.0
25	4962.5
26	5149.0
27	7170.0
28	9191.0
29	10871.5
30	12552.0
31	15068.0
32	17584.0
33	22797.0
34	28010.0
35	29195.5
36	30381.0
37	34219.5
38	38058.0
39	43486.0
40	48914.0
41	55526.5
42	62139.0
43	65319.5
44	68500.0
45	74777.5
46	81055.0
47	86558.0
48	92061.0
49	97529.0
50	102997.0
51	109318.5
52	115640.0
53	122968.0
54	130296.0
55	137482.5
56	144669.0
57	154244.5
58	163820.0
59	183096.5
60	202373.0
61	208873.0
62	215373.0
63	216480.0
64	217587.0
65	223396.0
66	229205.0
67	186025.5
68	142846.0
69	113826.0
70	84806.0
71	67418.5
72	50031.0
73	43023.5
74	36016.0
75	30133.5
76	24251.0
77	23969.5
78	23688.0
79	16707.0
80	9726.0
81	5913.0
82	2100.0
83	1644.0
84	1188.0
85	805.0
86	422.0
87	251.5
88	81.0
89	51.0
90	21.0
91	14.5
92	8.0
93	5.5
94	3.0
95	2.5
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2411012.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	11.502260270012167
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.25869990835578	8.311383731186126
2	8.807780855179645	2.0261877559501325
3	3.1552820286971635	1.0887862535810033
4	1.3744792246804869	0.6323847111199796
5	0.7931479269127103	0.456149694398529
6	0.5133090933460026	0.35425288743778155
7	0.40263987714320376	0.3241888063390797
8	0.3458489372078055	0.31824355918970193
9	0.3174803466337577	0.32865674198356454
>10	8.165768869354197	25.481660093700743
>50	2.4518674448024944	19.23287553074222
>100	1.3384445134068528	29.336812006955594
>500	0.05406380676423797	4.1031990131005145
>1k	0.01899539156581334	4.139543973894451
>5k	7.30591983300513E-4	0.6067297501323171
>10k+	0.001461183966601026	3.258945490288281
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTTGCCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	31417	1.303062780276498	No Hit
GTTGCCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG	18931	0.7851889579977204	No Hit
GTTGCCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	14488	0.600909493606834	No Hit
GTTGCCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT	12726	0.5278281485119112	No Hit
GTTGCCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGT	9196	0.38141660016623724	No Hit
GTTGCCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTT	5244	0.21750202819397	No Hit
GTTGCCGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCC	4787	0.19854733199171137	No Hit
GTTGCCGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGC	4414	0.1830766499710495	No Hit
GTTGCCGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT	4241	0.17590123981133235	No Hit
GTTGCCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC	3876	0.16076236866510826	No Hit
GTTGCCGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTC	3760	0.15595111098576034	No Hit
GTTGCCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTG	3338	0.13844808735916703	No Hit
GTTGCCGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC	3208	0.13305616064955297	No Hit
GTTGCCGGGGGGGCTGGCGAGATGGCTCAGTGGGTAAGAGCACCCGACTG	3025	0.12546598689678856	No Hit
GTTGCCGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCT	2967	0.12306035805711461	No Hit
GTTGCCGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCA	2700	0.11198617012275344	No Hit
GTTGCCGGGGGGGCTGGCGAGATGGCTCAGCGGGTAAGAGCACCCGATTG	2528	0.104852236322341	No Hit
GTTGCCGGGGGCAGTGGTGGCGCACGCCTTTCGTCCCAGCACTTGGGAGG	2522	0.10460337816651265	No Hit
GTTGCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGTTGCCGGGA	2500	0.10369089826180874	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.004064683211862903	0.0	0.0	0.0	0.0
8	0.006470312051536865	0.0	0.0	0.0	0.0
9	0.006843599285279376	0.0	0.0	0.0	0.0
10	0.013604245851949305	0.0	0.0	0.0	0.0
11	0.02160918319776094	0.0	0.0	0.0	0.0
12	0.0330151820065599	0.0	0.0	0.0	0.0
13	0.04321836639552188	0.0	0.0	0.0	0.0
14	0.05644932501372867	0.0	0.0	0.0	0.0
15	0.06681841483990955	0.0	0.0	0.0	0.0
16	0.07631650112069123	0.0	0.0	0.0	0.0
17	0.08519244201190206	0.0	0.0	0.0	0.0
18	0.09456609921476956	0.0	0.0	0.0	0.0
19	0.10825329778532831	0.0	0.0	0.0	0.0
20	0.11928600936038476	0.0	0.0	0.0	0.0
21	0.12828637932950976	0.0	0.0	0.0	0.0
22	0.13707936750211114	0.0	0.0	0.0	0.0
23	0.14748993368759675	0.0	0.0	0.0	0.0
24	0.1588959324963957	0.0	0.0	0.0	0.0
25	0.1690991168853577	0.0	0.0	0.0	0.0
26	0.17926082491501494	0.0	0.0	0.0	0.0
27	0.19460707785776263	0.0	0.0	0.0	0.0
28	0.23405109555655468	0.0	0.0	0.0	0.0
29	0.2450423307723064	0.0	0.0	0.0	0.0
30	0.26055448915227297	0.0	0.0	0.0	0.0
31	0.26947190640278856	0.0	0.0	0.0	0.0
32	0.2788040872463513	0.0	0.0	0.0	0.0
33	0.2873482172631244	0.0	0.0	0.0	0.0
34	0.3015331321453398	0.0	0.0	0.0	0.0
35	0.33301368885762495	0.0	0.0	0.0	0.0
36	0.3485258472375915	0.0	0.0	0.0	0.0
37	0.36076137323248497	0.0	0.0	0.0	0.0
38	0.3715452266517131	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATAACGC	20	7.8582024E-4	44.000004	17
GCGTAAC	20	7.8582024E-4	44.000004	33
CGTACCG	25	4.4442997E-5	44.0	32
TATACGC	25	4.4442997E-5	44.0	39
TGCCGGA	11035	0.0	42.62438	3
GTTGCCG	247915	0.0	42.042393	1
TTGCCGG	244660	0.0	41.990276	2
CGGGATA	3675	0.0	41.904762	6
TGCCGGG	227495	0.0	41.797047	3
CCGGGAT	28490	0.0	41.722008	5
CGGGATT	9705	0.0	41.71046	6
GCCGGGA	88345	0.0	41.659176	4
GCCGGGG	109310	0.0	41.55466	4
GCCGGAT	3305	0.0	41.537067	4
CCGGGGG	48350	0.0	41.456463	5
CGGGGGG	27265	0.0	41.43407	6
CGGGATC	8565	0.0	41.354347	6
TGCCGGT	3285	0.0	41.321156	3
CCGGGAC	13525	0.0	41.283546	5
CCGGGAA	16760	0.0	41.125294	5
>>END_MODULE