FastQCFastQC Report
Fri 17 Jun 2016
SRR1527593_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527593_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2151598
Sequences flagged as poor quality0
Sequence length52
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGCCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC269311.2516743369346877No Hit
GTTGCCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT159430.7409841429486363No Hit
GTTGCCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT124390.5781284422090001No Hit
GTTGCCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC105420.4899614147252414No Hit
GTTGCCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC82400.38297116840599404No Hit
GTTGCCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCT46530.21625786973217118No Hit
GTTGCCGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC46050.21402696972203913No Hit
GTTGCCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA41230.19162501545363028No Hit
GTTGCCGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTC39630.18418868208652361No Hit
GTTGCCGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG38670.17972688206625959No Hit
GTTGCCGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC37970.17647348621815043No Hit
GTTGCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGTTGCCGGGAAG31580.1467746298332681No Hit
GTTGCCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCT29900.13896647979780608No Hit
GTTGCCGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT27390.1273007318281575No Hit
GTTGCCGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAG27040.12567403390410292No Hit
GTTGCCGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCT26310.12228120680536049No Hit
GTTGCCGGGGGCAGTGGTGGCGCACGCCTTTCGTCCCAGCACTTGGGAGGCA24010.11159147759014464No Hit
GTTGCCGGGGGGGCTGGCGAGATGGCTCAGCGGGTAAGAGCACCCGATTGCT23630.1098253484154568No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGATAA351.02012564E-746.00000441
TCGGTAC351.02012564E-746.00000432
ATCACGC253.4181572E-546.028
CGCTATA301.8621649E-646.031
ATGCGTT206.312688E-446.030
ATACGTC206.312688E-446.031
TAGGACG206.312688E-446.036
CGACTAT206.312688E-446.016
TATACGC405.6152203E-946.039
AATCGTG301.8621649E-646.035
TGCCGGA94950.044.2801483
CGGGATA33000.044.0484856
GTTGCCG2224550.043.7667431
TGCCGGT27450.043.7377053
TTGCCGG2196650.043.6860242
GCCGGAT27550.043.662434
CCGGGAT252050.043.563585
TGCCGGG2052200.043.48843
CGGGATT86200.043.46526
GCCGGGA782450.043.2515834
Produced by FastQC (version 0.11.5)