FastQCFastQC Report
Fri 17 Jun 2016
SRR1527592_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527592_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2355878
Sequences flagged as poor quality0
Sequence length52
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGCCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC287431.2200546887402488No Hit
GTTGCCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT173070.7346305708529899No Hit
GTTGCCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT132160.5609798130463463No Hit
GTTGCCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC110980.47107702521098294No Hit
GTTGCCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC89070.37807560493370196No Hit
GTTGCCGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC49940.21198041664296707No Hit
GTTGCCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCT47540.20179313190241602No Hit
GTTGCCGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG44830.19028998954954374No Hit
GTTGCCGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTC44460.18871944981870878No Hit
GTTGCCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA40860.17343852270788215No Hit
GTTGCCGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC40530.1720377710560564No Hit
GTTGCCGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT33120.14058452941960492No Hit
GTTGCCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCT32560.13820749631347634No Hit
GTTGCCGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCT30390.12899649302722807No Hit
GTTGCCGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAG27130.11515876458797951No Hit
GTTGCCGGGGGGGCTGGCGAGATGGCTCAGCGGGTAAGAGCACCCGATTGCT26630.11303641360036469No Hit
GTTGCCGGGGGCAGTGGTGGCGCACGCCTTTCGTCCCAGCACTTGGGAGGCA24950.10590531428197895No Hit
GTTGCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGTTGCCGGGAAG24930.10582042024247434No Hit
GTTGCCGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCGCC24350.10335849309684117No Hit
GTTGCCGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCT23580.10009007257591436No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGTAC206.3128094E-446.019
TGCCGGA105700.044.672663
CGGGATA35650.043.9354826
GTTGCCG2430500.043.8452571
TGCCGGT31800.043.830193
TTGCCGG2398500.043.7848662
CGGGATT92500.043.762166
TGCCGGG2238300.043.563643
CCGGGAT277200.043.5440145
GCCGGAT30400.043.427634
CCGGGGG479700.043.39655
GCCGGGA858100.043.3518264
GCCGGGG1093300.043.3408974
CGGGGGG265950.042.9817666
CCGGGGC240350.042.8229645
CGGGAGT70850.042.7537046
CCGGGAC133750.042.5435525
GCCGGGT108500.042.417514
CGGGATC87150.042.4107866
CGGGGCT146000.042.2506876
Produced by FastQC (version 0.11.5)