##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527592_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2355878
Sequences flagged as poor quality	0
Sequence length	52
%GC	57
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.44814035361763	31.0	31.0	33.0	27.0	34.0
2	30.521830502258606	31.0	31.0	33.0	28.0	34.0
3	30.597213013577104	31.0	31.0	31.0	28.0	34.0
4	35.37386570951467	37.0	35.0	37.0	33.0	37.0
5	35.77415893352712	37.0	35.0	37.0	35.0	37.0
6	36.212628582634586	37.0	36.0	37.0	35.0	37.0
7	36.122613310196876	37.0	35.0	37.0	35.0	37.0
8	36.31387448755835	37.0	37.0	37.0	35.0	37.0
9	38.2190015781802	39.0	39.0	39.0	37.0	39.0
10	36.45641115541637	39.0	35.0	39.0	31.0	39.0
11	36.92971834704514	39.0	37.0	39.0	34.0	39.0
12	36.73721432094531	39.0	35.0	39.0	33.0	39.0
13	36.764599440208706	39.0	35.0	39.0	33.0	39.0
14	37.48342528772712	40.0	36.0	40.0	33.0	40.0
15	37.518225052400844	40.0	36.0	40.0	33.0	40.0
16	37.47112032117113	39.0	36.0	40.0	33.0	40.0
17	37.42491504228997	39.0	36.0	40.0	33.0	40.0
18	37.33414633525165	39.0	36.0	40.0	33.0	40.0
19	37.119323666166075	39.0	36.0	40.0	32.0	40.0
20	36.96792490952418	39.0	36.0	40.0	31.0	40.0
21	37.03648406241749	39.0	36.0	40.0	31.0	40.0
22	37.02773488270615	39.0	35.0	40.0	32.0	40.0
23	36.99103518942832	39.0	35.0	40.0	32.0	40.0
24	37.07605996575374	39.0	35.0	40.0	32.0	40.0
25	37.075487355457284	39.0	35.0	40.0	33.0	40.0
26	37.00374976972492	39.0	35.0	40.0	33.0	40.0
27	36.878400324634804	39.0	35.0	40.0	32.0	40.0
28	36.694118286261	38.0	35.0	40.0	31.0	40.0
29	36.658486560000135	38.0	35.0	40.0	31.0	40.0
30	36.56671652776587	38.0	35.0	40.0	31.0	40.0
31	36.46916308909035	38.0	35.0	40.0	31.0	40.0
32	36.368569594860176	38.0	35.0	40.0	31.0	40.0
33	36.14742698900368	38.0	35.0	40.0	31.0	40.0
34	35.718617432651435	38.0	34.0	40.0	29.0	40.0
35	35.59604317371273	38.0	34.0	40.0	29.0	40.0
36	35.655351847591426	38.0	34.0	40.0	29.0	40.0
37	35.66475768269834	38.0	34.0	40.0	30.0	40.0
38	35.65492440610252	38.0	34.0	40.0	30.0	40.0
39	35.53968923687899	37.0	34.0	40.0	30.0	40.0
40	35.81512370334966	38.0	35.0	40.0	30.0	40.0
41	35.931503244225716	38.0	35.0	40.0	31.0	40.0
42	35.90027072709198	38.0	35.0	40.0	31.0	40.0
43	35.897204354385075	37.0	35.0	40.0	31.0	40.0
44	35.762364180148545	37.0	35.0	40.0	31.0	40.0
45	35.64077172077671	37.0	34.0	40.0	30.0	40.0
46	35.284504545651345	37.0	34.0	40.0	30.0	40.0
47	35.266336372256966	36.0	34.0	40.0	30.0	40.0
48	35.160214153704054	36.0	34.0	40.0	29.0	40.0
49	35.06279187631957	36.0	34.0	40.0	29.0	40.0
50	34.89312052661471	35.0	34.0	39.0	29.0	40.0
51	34.78528005270222	35.0	34.0	39.0	29.0	40.0
52	34.298749341010016	35.0	33.0	39.0	28.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	3.0
11	13.0
12	36.0
13	59.0
14	91.0
15	175.0
16	283.0
17	500.0
18	770.0
19	1201.0
20	1970.0
21	2903.0
22	4476.0
23	6484.0
24	9092.0
25	12650.0
26	17040.0
27	21918.0
28	27522.0
29	34702.0
30	44369.0
31	58668.0
32	80940.0
33	122661.0
34	222237.0
35	200675.0
36	308278.0
37	463878.0
38	653591.0
39	58693.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	100.0	0.0	0.0	0.0
2	0.0	0.0	100.0	0.0
3	0.0	0.0	100.0	0.0
4	100.0	0.0	0.0	0.0
5	0.0	0.0	0.0	100.0
6	0.0	0.0	0.0	100.0
7	98.2963039682021	0.7036442464338136	0.3535412275168748	0.646510557847223
8	97.87663028391114	1.0871955169155618	0.4376712206659258	0.5985029785073761
9	92.11096669691725	4.618575325207842	1.5000776780461467	1.7703802998287685
10	46.84699292577969	37.69032182481436	6.28636117829531	9.176324071110644
11	36.74596052936528	19.644565635402174	24.487600801060157	19.1218730341724
12	30.863567638052565	16.68800336859549	26.777193046499015	25.67123594685293
13	27.894568394458457	16.76181873594473	28.413355869871022	26.930256999725792
14	25.966879439427675	18.473961724673348	31.487793510529833	24.07136532536914
15	29.135549463936588	18.17475268243941	29.7976380780329	22.892059775591093
16	34.600221233866954	19.757559601982784	24.331650450490223	21.310568713660043
17	31.72634576153774	20.824253208357987	22.597222776391646	24.85217825371263
18	30.80401446934009	21.286501253460493	22.6296522994824	25.27983197771701
19	31.81442332752375	23.414243012583842	22.361726710805907	22.4096069490865
20	32.06524276724007	24.547408651891143	22.55961471689111	20.827733863977677
21	31.03225209454819	23.277351373882688	25.146972805892325	20.543423725676796
22	29.583323075303557	21.12227373403886	26.404508213073854	22.88989497758373
23	29.2004085101181	22.830087126752744	25.876000370138012	22.09350399299115
24	29.89170067380399	21.466009699992956	23.335079320745812	25.307210305457247
25	27.368946948865773	21.166418634581248	23.543961104946863	27.92067331160612
26	25.48833173874029	22.47140980984584	24.943099770022048	27.097158681391818
27	23.40830042981852	22.062729903670732	24.43012753631555	30.098842130195198
28	25.61936568871563	25.63850929462392	23.10544094388589	25.636684072774568
29	28.667740859246532	23.515394260653565	23.245813238206733	24.57105164189317
30	28.28945301921407	21.864926791625034	24.590747059058238	25.254873130102663
31	31.386175345242833	21.565165938134317	24.199767560119838	22.848891156503008
32	32.75606801370869	20.734350420522624	23.5780036147882	22.931577950980483
33	32.169619988810965	20.845137142076116	24.558572218086	22.42667065102692
34	27.532367974912113	23.743929014999928	27.28367088618341	21.440032123904547
35	25.80226989682827	27.77109001399903	25.909703303821335	20.516936785351366
36	26.836788662231235	27.44963873341489	23.50919699576973	22.204375608584144
37	28.272771340451413	28.428933926120116	21.710462086746425	21.587832646682042
38	28.957059745878183	26.586648374830958	21.355987024795002	23.100304854495864
39	28.373837694481637	24.37363055302524	21.59407235858563	25.658459393907496
40	25.928252651453086	24.91593367738058	21.93212042389292	27.223693247273417
41	24.21169517267023	25.321430057074263	22.4124508994099	28.054423870845604
42	22.693110594012083	23.846693249820238	24.03286587845381	29.42733027771387
43	23.97781209383508	21.883858162434557	24.370574367603076	29.767755376127287
44	25.81330612196387	21.319397693768522	23.474730015730866	29.392566168536742
45	26.509946610138556	23.50936678384874	22.673415176847016	27.307271429165684
46	25.43820180841283	24.647753406585572	23.36725416171805	26.54679062328355
47	24.97951931296952	23.747027647441847	25.094720524577248	26.178732515011387
48	26.669420063347932	22.362618098220704	25.882706999258875	25.08525483917249
49	27.156542062025284	21.95656990727024	25.397749798588894	25.489138232115582
50	25.315147898150926	21.934242774880534	26.417030083900777	26.333579243067767
51	24.011727262617164	22.288420707693692	26.563005384828926	27.136846644860217
52	23.512974780527685	22.469924164154513	27.710305881713737	26.306795173604065
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	7.5
8	15.0
9	60.0
10	105.0
11	175.0
12	245.0
13	572.0
14	1890.5
15	2882.0
16	3126.5
17	3371.0
18	3242.5
19	3114.0
20	3213.5
21	3313.0
22	3691.5
23	4070.0
24	3931.5
25	3793.0
26	5266.5
27	6740.0
28	8251.5
29	9763.0
30	11446.5
31	13130.0
32	17421.5
33	21713.0
34	24220.0
35	26727.0
36	28741.0
37	30755.0
38	34570.0
39	45314.5
40	52244.0
41	56019.5
42	59795.0
43	63151.0
44	66507.0
45	72567.0
46	78627.0
47	83635.0
48	88643.0
49	94142.5
50	99642.0
51	104570.5
52	109499.0
53	113666.5
54	117834.0
55	128282.0
56	138730.0
57	143945.5
58	149161.0
59	164117.0
60	179073.0
61	195249.0
62	211425.0
63	210782.5
64	210673.0
65	211206.0
66	180083.5
67	148961.0
68	121448.5
69	93936.0
70	74972.0
71	56008.0
72	47808.0
73	39608.0
74	34314.0
75	29020.0
76	27861.5
77	26703.0
78	19711.5
79	12720.0
80	8868.5
81	5017.0
82	3090.0
83	1163.0
84	889.5
85	616.0
86	472.0
87	328.0
88	239.5
89	88.0
90	25.0
91	24.0
92	23.0
93	24.5
94	26.0
95	26.5
96	27.0
97	13.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	2355878.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	10.054693632347119
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.555573268539	6.993599796374326
2	9.171160839445799	1.8442642498721387
3	3.310797287164504	0.9986715720373515
4	1.5176365227034523	0.610374811241753
5	0.8614733211200151	0.43309251581511704
6	0.5571475937045519	0.3361169017619206
7	0.4420031464538503	0.31109443554888433
8	0.37217730573417723	0.2993703028855631
9	0.3470851011095442	0.3140850920407821
>10	9.308413788869895	25.55195188300065
>50	2.873408241958949	19.776734191958553
>100	1.5931305946369503	30.434523905910503
>500	0.06537226527420469	4.314564478733531
>1k	0.02249824713982369	4.398135237083865
>5k	4.244952290532772E-4	0.38016585527396757
>10k+	0.0016979809162131088	3.003254770461149
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTTGCCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	28743	1.2200546887402488	No Hit
GTTGCCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	17307	0.7346305708529899	No Hit
GTTGCCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT	13216	0.5609798130463463	No Hit
GTTGCCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	11098	0.47107702521098294	No Hit
GTTGCCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC	8907	0.37807560493370196	No Hit
GTTGCCGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC	4994	0.21198041664296707	No Hit
GTTGCCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCT	4754	0.20179313190241602	No Hit
GTTGCCGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG	4483	0.19028998954954374	No Hit
GTTGCCGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTC	4446	0.18871944981870878	No Hit
GTTGCCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA	4086	0.17343852270788215	No Hit
GTTGCCGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC	4053	0.1720377710560564	No Hit
GTTGCCGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT	3312	0.14058452941960492	No Hit
GTTGCCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCT	3256	0.13820749631347634	No Hit
GTTGCCGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCT	3039	0.12899649302722807	No Hit
GTTGCCGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAG	2713	0.11515876458797951	No Hit
GTTGCCGGGGGGGCTGGCGAGATGGCTCAGCGGGTAAGAGCACCCGATTGCT	2663	0.11303641360036469	No Hit
GTTGCCGGGGGCAGTGGTGGCGCACGCCTTTCGTCCCAGCACTTGGGAGGCA	2495	0.10590531428197895	No Hit
GTTGCCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGTTGCCGGGAAG	2493	0.10582042024247434	No Hit
GTTGCCGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCGCC	2435	0.10335849309684117	No Hit
GTTGCCGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCT	2358	0.10009007257591436	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0032684205209268053	0.0	0.0	0.0	0.0
8	0.006069923824578352	0.0	0.0	0.0	0.0
9	0.006918864219624276	0.0	0.0	0.0	0.0
10	0.011885165530642929	0.0	0.0	0.0	0.0
11	0.01961052312556083	0.0	0.0	0.0	0.0
12	0.0288215264118091	0.0	0.0	0.0	0.0
13	0.03726848334251604	0.0	0.0	0.0	0.0
14	0.050087483307709485	0.0	0.0	0.0	0.0
15	0.06052945016677434	0.0	0.0	0.0	0.0
16	0.06918864219624275	0.0	0.0	0.0	0.0
17	0.07814496336397725	0.0	0.0	0.0	0.0
18	0.08790777790700537	0.0	0.0	0.0	0.0
19	0.09958070833888683	0.0	0.0	0.0	0.0
20	0.10947086394117182	0.0	0.0	0.0	0.0
21	0.11804516193113565	0.0	0.0	0.0	0.0
22	0.1242424268149709	0.0	0.0	0.0	0.0
23	0.13362321818022835	0.0	0.0	0.0	0.0
24	0.1442774201380547	0.0	0.0	0.0	0.0
25	0.1528517181280185	0.0	0.0	0.0	0.0
26	0.16112888697971628	0.0	0.0	0.0	0.0
27	0.1729291584708546	0.0	0.0	0.0	0.0
28	0.2073536914899668	0.0	0.0	0.0	0.0
29	0.21584309544042604	0.0	0.0	0.0	0.0
30	0.23095423447224347	0.0	0.0	0.0	0.0
31	0.2396558735214642	0.0	0.0	0.0	0.0
32	0.24691431389910684	0.0	0.0	0.0	0.0
33	0.25336626090145586	0.0	0.0	0.0	0.0
34	0.26457227411606205	0.0	0.0	0.0	0.0
35	0.29785073760186226	0.0	0.0	0.0	0.0
36	0.3117733600806154	0.0	0.0	0.0	0.0
37	0.32217287991992793	0.0	0.0	0.0	0.0
38	0.33108675406791016	0.0	0.0	0.0	0.0
39	0.3378782772282775	0.0	0.0	0.0	0.0
40	0.3453065056849294	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTCGTAC	20	6.3128094E-4	46.0	19
TGCCGGA	10570	0.0	44.67266	3
CGGGATA	3565	0.0	43.935482	6
GTTGCCG	243050	0.0	43.845257	1
TGCCGGT	3180	0.0	43.83019	3
TTGCCGG	239850	0.0	43.784866	2
CGGGATT	9250	0.0	43.76216	6
TGCCGGG	223830	0.0	43.56364	3
CCGGGAT	27720	0.0	43.544014	5
GCCGGAT	3040	0.0	43.42763	4
CCGGGGG	47970	0.0	43.3965	5
GCCGGGA	85810	0.0	43.351826	4
GCCGGGG	109330	0.0	43.340897	4
CGGGGGG	26595	0.0	42.981766	6
CCGGGGC	24035	0.0	42.822964	5
CGGGAGT	7085	0.0	42.753704	6
CCGGGAC	13375	0.0	42.543552	5
GCCGGGT	10850	0.0	42.41751	4
CGGGATC	8715	0.0	42.410786	6
CGGGGCT	14600	0.0	42.250687	6
>>END_MODULE