FastQCFastQC Report
Fri 17 Jun 2016
SRR1527591_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527591_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences743964
Sequences flagged as poor quality0
Sequence length50
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC82201.1048921722018807No Hit
CACAAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG37220.5002930249313139No Hit
CACAAGGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT31970.42972509422498933No Hit
CACAAGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC31610.42488615040512717No Hit
CACAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGT27650.37165776838664233No Hit
CACAAGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT27090.36413052244463445No Hit
CACAAGGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTG26900.361576635428596No Hit
CACAAGGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTAT25930.3485383701361894No Hit
CACAAGGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC23530.31627874467044104No Hit
CACAAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCC17580.23630175653660662No Hit
CACAAGGGGGAAGATACTTTATGAACTTTACAGTTAAAAGATTAAAAATC17150.23052190697399338No Hit
CACAAGGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCA17010.22864009548849137No Hit
CACAAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTG16950.22783360485184767No Hit
CACAAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTC14240.1914071110967735No Hit
CACAAGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCT13340.17930975154711787No Hit
CACAAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAG12880.17312665666618277No Hit
CACAAGGGGGGGGGCTGGTGAGATGGCTCAGTGGTTAAGAGCTCTGACTA12020.1615669575409563No Hit
CACAAGGGGCTCTTCATCTCTAACCATGCCTACTAACCAGAGCTGAACTA11460.15403971159894833No Hit
CACAAGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCT10790.14503389948976025No Hit
CACAAGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGA10730.14422740885311655No Hit
CACAAGGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCG10190.13696899312332317No Hit
CACAAGGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCG10060.13522159674392847No Hit
CACAAGGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCAACGACTGC9680.13011382271185165No Hit
CACAAGGGGGGCCTCTCCTTCCTGCGGCGCCTTAGGGACCATGGCCGATC9420.12661902995306223No Hit
CACAAGGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGC8610.11573140635837219No Hit
CACAAGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAA8500.11425284019119204No Hit
CACAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAACTGC8000.1075320848858278No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA600.044.043
GCGGTAT551.8189894E-1244.013
TTAGACG207.8546896E-444.020
ATAGCGT408.305506E-944.015
TACGGTG454.783942E-1044.035
CCTAATA207.8546896E-444.017
TCGATTG207.8546896E-444.013
ACTTACG207.8546896E-444.018
TCGGCAT207.8546896E-444.021
CGTTAGT207.8546896E-444.019
AAACGTA207.8546896E-444.042
GTTCGAT207.8546896E-444.011
GCGATTA302.526509E-644.09
CATCGAA207.8546896E-444.025
CACGTTA207.8546896E-444.017
GATTCGT454.783942E-1044.09
TATACGT502.7284841E-1144.023
TATCGTA207.8546896E-444.013
AGTCGAC207.8546896E-444.011
GTACGAC302.526509E-644.031
Produced by FastQC (version 0.11.5)