##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527591_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	743964
Sequences flagged as poor quality	0
Sequence length	50
%GC	56
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.58204966907001	31.0	31.0	33.0	30.0	34.0
2	30.866727422294627	31.0	31.0	33.0	28.0	34.0
3	32.136434021001016	33.0	31.0	34.0	30.0	34.0
4	35.43868386104704	35.0	35.0	37.0	33.0	37.0
5	35.78091009780043	37.0	35.0	37.0	35.0	37.0
6	35.975216542735936	37.0	35.0	37.0	35.0	37.0
7	36.54473469146357	37.0	37.0	37.0	35.0	37.0
8	36.70448704507207	37.0	37.0	37.0	35.0	37.0
9	38.66271083009393	39.0	39.0	39.0	39.0	39.0
10	37.30506449236791	39.0	37.0	39.0	34.0	39.0
11	36.20265631132689	37.0	35.0	39.0	33.0	39.0
12	36.117125291008705	37.0	35.0	39.0	33.0	39.0
13	36.20878429601432	38.0	35.0	39.0	33.0	39.0
14	36.93794457796345	39.0	35.0	40.0	33.0	40.0
15	37.058025657155454	39.0	35.0	40.0	33.0	40.0
16	37.18935996903076	39.0	35.0	40.0	33.0	40.0
17	37.10690839879349	39.0	35.0	40.0	33.0	40.0
18	36.983582538940055	38.0	35.0	40.0	33.0	40.0
19	36.88308305240576	38.0	35.0	40.0	33.0	40.0
20	36.72036550155653	38.0	35.0	40.0	33.0	40.0
21	36.596331274093906	38.0	35.0	40.0	33.0	40.0
22	36.43723083375002	38.0	35.0	40.0	32.0	40.0
23	36.23130957949578	38.0	35.0	40.0	32.0	40.0
24	36.089246253850995	38.0	34.0	40.0	32.0	40.0
25	35.87584883139507	37.0	34.0	39.0	31.0	40.0
26	35.343422531197746	36.0	34.0	39.0	30.0	40.0
27	35.24813700662936	36.0	34.0	38.0	30.0	40.0
28	35.0600889290342	36.0	34.0	38.0	30.0	40.0
29	34.7456731777344	36.0	33.0	38.0	30.0	40.0
30	34.42404471184089	35.0	33.0	38.0	29.0	39.0
31	34.2453142894011	35.0	33.0	38.0	29.0	39.0
32	34.16665994591136	35.0	33.0	38.0	29.0	39.0
33	33.54010274690711	35.0	32.0	38.0	27.0	39.0
34	33.05238828760531	34.0	31.0	37.0	25.0	38.0
35	32.925418434225314	34.0	31.0	37.0	25.0	38.0
36	32.67405008844514	34.0	31.0	37.0	25.0	38.0
37	32.52364227301321	34.0	31.0	36.0	25.0	38.0
38	32.06270195869693	33.0	30.0	36.0	24.0	38.0
39	31.334629094956206	33.0	29.0	35.0	23.0	38.0
40	31.027198896720808	33.0	29.0	35.0	23.0	38.0
41	30.809317386325144	32.0	29.0	35.0	23.0	37.0
42	30.39113720556371	32.0	28.0	34.0	22.0	37.0
43	29.849543795129872	31.0	27.0	34.0	21.0	37.0
44	29.28086170836223	31.0	26.0	34.0	20.0	36.0
45	28.90425477576872	31.0	26.0	34.0	20.0	36.0
46	28.72069481856649	30.0	26.0	33.0	20.0	36.0
47	28.279165389723158	30.0	24.0	33.0	20.0	35.0
48	27.890232591899608	30.0	24.0	33.0	18.0	35.0
49	27.386784844427957	29.0	24.0	32.0	18.0	34.0
50	31.16183713190423	33.0	29.0	35.0	24.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	2.0
13	3.0
14	14.0
15	49.0
16	118.0
17	196.0
18	333.0
19	539.0
20	856.0
21	1304.0
22	2002.0
23	2868.0
24	3829.0
25	5152.0
26	6934.0
27	9482.0
28	13102.0
29	18622.0
30	27560.0
31	46288.0
32	76738.0
33	89926.0
34	93761.0
35	174470.0
36	152454.0
37	16983.0
38	378.0
39	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	0.0	100.0
2	0.0	100.0	0.0	0.0
3	0.0	0.0	0.0	100.0
4	0.0	100.0	0.0	0.0
5	0.0	100.0	0.0	0.0
6	100.0	0.0	0.0	0.0
7	98.82292691581851	1.0694065841895575	0.035082342694001324	0.07258415729793377
8	99.44849481964181	0.41346086638600793	0.09140227215295363	0.04664204181922781
9	97.86185890715143	1.180567876940282	0.4169556591447974	0.5406175567634993
10	58.65969321096182	31.041152528885807	3.555279556537682	6.743874703614691
11	39.541563839110495	24.718669182917452	20.46147394228753	15.278293035684523
12	32.749434112403286	17.57087708545037	25.21882779274266	24.460861009403683
13	25.835793129775098	16.877563968148994	28.310912893634637	28.975730008441268
14	24.30682129780473	18.877526331919288	29.180981875467094	27.634670494808887
15	26.174519197165452	19.7080772725562	27.133839809453146	26.9835637208252
16	32.57845809743482	22.050260496475634	22.780403352850406	22.590878053239134
17	31.964046647418424	22.02687226801297	22.592356619406313	23.416724465162293
18	32.52052518670258	21.703603937824948	21.900253238059904	23.875617637412564
19	30.076724142566036	23.855052126178148	20.939050814286713	25.1291729169691
20	31.455151055696245	22.6510960207752	22.492889440886923	23.400863482641636
21	30.691270007688544	23.263087998881666	24.035840443892447	22.009801549537343
22	30.69624336661451	20.295874531563353	25.563736955013955	23.44414514680818
23	30.302541520826274	20.709335397949364	24.261523407046578	24.72659967417778
24	29.951718093886264	20.913108698808006	24.270663634261876	24.864509573043858
25	28.816985768128568	22.31384851955202	22.05469619497718	26.81446951734224
26	25.880015699684396	23.156227989526375	24.271873370216838	26.69188294057239
27	25.693447532407482	20.820631105806196	24.12939335774312	29.35652800404321
28	27.88132221451576	23.46887752633192	22.44167728546005	26.208122973692277
29	30.438031947782417	21.697555258050123	21.911812937185132	25.952599856982328
30	27.673919705792215	21.49942739164798	23.80935099010167	27.01730191245813
31	31.836621126828717	22.398933281717934	21.886408482130857	23.878037109322495
32	33.44758617352453	21.189331741858478	21.9628906775059	23.400191407111095
33	33.05912651687447	19.96548220075165	23.284863246071048	23.690528036302833
34	29.024388276852104	22.091391518944466	27.492593727653485	21.39162647654994
35	27.047948556650592	26.452489636595317	24.629014307143894	21.870547499610197
36	27.986703657703867	27.420412815673878	22.20994564253109	22.382937884091167
37	31.19680522175804	26.033920996177233	20.81740514325962	21.951868638805102
38	30.558467882854544	26.59550730949347	20.30111672070154	22.544908086950443
39	31.68997424606567	22.538321746751187	21.42980036668441	24.34190364049873
40	28.541434800608634	23.213219994515864	21.36675968192009	26.87858552295541
41	26.14239398680581	24.93561516417461	21.41501470501261	27.506976144006966
42	24.65253695071267	21.75266545155411	23.91997462242797	29.67482297530526
43	25.433623132302102	20.80463570817943	25.942921969342603	27.81881919017587
44	26.153550440612715	20.06508379437715	24.58425407681017	29.197111688199968
45	28.075955288159104	20.636347995333107	24.231414423278547	27.056282293229245
46	27.44151598733272	23.59200176352619	22.706206214279185	26.260276034861903
47	25.42421407487459	22.576898882203977	25.46023732331134	26.538649719610092
48	28.256609190767296	21.82524960885204	24.62430977843014	25.293831421950525
49	27.74999865584894	21.3595012661903	25.49693264727863	25.39356743068213
50	25.029033662919176	21.970955583872335	25.222994661031983	27.777016092176503
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.5
6	1.0
7	1.0
8	1.0
9	1.5
10	2.0
11	52.0
12	102.0
13	291.5
14	481.0
15	626.5
16	772.0
17	637.5
18	503.0
19	539.0
20	575.0
21	568.5
22	562.0
23	860.5
24	1159.0
25	1393.0
26	1627.0
27	2351.0
28	3075.0
29	4492.0
30	5909.0
31	6735.0
32	7561.0
33	7521.0
34	7481.0
35	10591.5
36	13702.0
37	12622.0
38	11542.0
39	13277.0
40	15012.0
41	18481.5
42	21951.0
43	22722.0
44	23493.0
45	23830.5
46	24168.0
47	24431.5
48	24695.0
49	28965.5
50	33236.0
51	35191.0
52	37146.0
53	38633.5
54	40121.0
55	43609.5
56	47098.0
57	55744.0
58	64390.0
59	67680.5
60	70971.0
61	70192.5
62	69414.0
63	68836.5
64	68259.0
65	59015.0
66	49771.0
67	41159.5
68	32548.0
69	26575.5
70	20603.0
71	18296.5
72	15990.0
73	13556.5
74	11123.0
75	11365.5
76	11608.0
77	8218.0
78	4828.0
79	3087.0
80	1346.0
81	1023.5
82	701.0
83	545.5
84	390.0
85	209.5
86	29.0
87	23.5
88	18.0
89	9.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	743964.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	11.203633509148291
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.15752660435987	7.860192159835691
2	9.430000839821957	2.1130054680065165
3	3.127736919773008	1.051260544865074
4	1.4300968194742714	0.6408912259195337
5	0.7618384902400691	0.42676796189062915
6	0.4858969898381543	0.32662870784070197
7	0.4199109788724791	0.3293170099628477
8	0.35752420486856784	0.32044561295976687
9	0.34912598529111827	0.35203316289497877
>10	9.368813811471968	28.806501389852198
>50	2.733020599632878	20.632584372362103
>100	1.317320728005663	28.062917022866696
>500	0.03479262396371969	2.5112774274024012
>1k	0.025194658732348746	5.4612857611389805
>5k	0.0011997456539213686	1.1048921722018807
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CACAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	8220	1.1048921722018807	No Hit
CACAAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG	3722	0.5002930249313139	No Hit
CACAAGGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT	3197	0.42972509422498933	No Hit
CACAAGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC	3161	0.42488615040512717	No Hit
CACAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGT	2765	0.37165776838664233	No Hit
CACAAGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT	2709	0.36413052244463445	No Hit
CACAAGGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTG	2690	0.361576635428596	No Hit
CACAAGGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTAT	2593	0.3485383701361894	No Hit
CACAAGGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC	2353	0.31627874467044104	No Hit
CACAAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCC	1758	0.23630175653660662	No Hit
CACAAGGGGGAAGATACTTTATGAACTTTACAGTTAAAAGATTAAAAATC	1715	0.23052190697399338	No Hit
CACAAGGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCA	1701	0.22864009548849137	No Hit
CACAAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTG	1695	0.22783360485184767	No Hit
CACAAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTC	1424	0.1914071110967735	No Hit
CACAAGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCT	1334	0.17930975154711787	No Hit
CACAAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAG	1288	0.17312665666618277	No Hit
CACAAGGGGGGGGGCTGGTGAGATGGCTCAGTGGTTAAGAGCTCTGACTA	1202	0.1615669575409563	No Hit
CACAAGGGGCTCTTCATCTCTAACCATGCCTACTAACCAGAGCTGAACTA	1146	0.15403971159894833	No Hit
CACAAGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCT	1079	0.14503389948976025	No Hit
CACAAGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGA	1073	0.14422740885311655	No Hit
CACAAGGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCG	1019	0.13696899312332317	No Hit
CACAAGGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCG	1006	0.13522159674392847	No Hit
CACAAGGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCAACGACTGC	968	0.13011382271185165	No Hit
CACAAGGGGGGCCTCTCCTTCCTGCGGCGCCTTAGGGACCATGGCCGATC	942	0.12661902995306223	No Hit
CACAAGGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGC	861	0.11573140635837219	No Hit
CACAAGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAA	850	0.11425284019119204	No Hit
CACAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAACTGC	800	0.1075320848858278	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.05080891010855364	0.0	0.0	0.0	0.0
6	0.05080891010855364	0.0	0.0	0.0	0.0
7	0.05161540074519735	0.0	0.0	0.0	0.0
8	0.05242189138184106	0.0	0.0	0.0	0.0
9	0.05322838201848477	0.0	0.0	0.0	0.0
10	0.06720755305364239	0.0	0.0	0.0	0.0
11	0.0767510255872596	0.0	0.0	0.0	0.0
12	0.08333736578651656	0.0	0.0	0.0	0.0
13	0.09005812109188079	0.0	0.0	0.0	0.0
14	0.09691329150335232	0.0	0.0	0.0	0.0
15	0.10484378276368211	0.0	0.0	0.0	0.0
16	0.11398400997897748	0.0	0.0	0.0	0.0
17	0.12231774655762913	0.0	0.0	0.0	0.0
18	0.1278287659080278	0.0	0.0	0.0	0.0
19	0.1367001629111086	0.0	0.0	0.0	0.0
20	0.14395857864090197	0.0	0.0	0.0	0.0
21	0.15054491884015894	0.0	0.0	0.0	0.0
22	0.15659359861498676	0.0	0.0	0.0	0.0
23	0.16277669349592186	0.0	0.0	0.0	0.0
24	0.1677500524218914	0.0	0.0	0.0	0.0
25	0.1756805436822212	0.0	0.0	0.0	0.0
26	0.18280454430590728	0.0	0.0	0.0	0.0
27	0.19073503556623708	0.0	0.0	0.0	0.0
28	0.19732137576549402	0.0	0.0	0.0	0.0
29	0.20337005554032184	0.0	0.0	0.0	0.0
30	0.20928432020904236	0.0	0.0	0.0	0.0
31	0.22003752869762516	0.0	0.0	0.0	0.0
32	0.2275647746396331	0.0	0.0	0.0	0.0
33	0.2345543601572119	0.0	0.0	0.0	0.0
34	0.24342575716029272	0.0	0.0	0.0	0.0
35	0.2543133807549828	0.0	0.0	0.0	0.0
36	0.2654698345618874	0.0	0.0	0.0	0.0
37	0.2758197977321483	0.0	0.0	0.0	0.0
38	0.2849600249474437	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTATCA	60	0.0	44.0	43
GCGGTAT	55	1.8189894E-12	44.0	13
TTAGACG	20	7.8546896E-4	44.0	20
ATAGCGT	40	8.305506E-9	44.0	15
TACGGTG	45	4.783942E-10	44.0	35
CCTAATA	20	7.8546896E-4	44.0	17
TCGATTG	20	7.8546896E-4	44.0	13
ACTTACG	20	7.8546896E-4	44.0	18
TCGGCAT	20	7.8546896E-4	44.0	21
CGTTAGT	20	7.8546896E-4	44.0	19
AAACGTA	20	7.8546896E-4	44.0	42
GTTCGAT	20	7.8546896E-4	44.0	11
GCGATTA	30	2.526509E-6	44.0	9
CATCGAA	20	7.8546896E-4	44.0	25
CACGTTA	20	7.8546896E-4	44.0	17
GATTCGT	45	4.783942E-10	44.0	9
TATACGT	50	2.7284841E-11	44.0	23
TATCGTA	20	7.8546896E-4	44.0	13
AGTCGAC	20	7.8546896E-4	44.0	11
GTACGAC	30	2.526509E-6	44.0	31
>>END_MODULE