FastQCFastQC Report
Fri 17 Jun 2016
SRR1527590_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527590_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences667916
Sequences flagged as poor quality0
Sequence length52
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC75561.1312799813150156No Hit
CACAAGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA34260.5129387527772954No Hit
CACAAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT33590.5029075512489595No Hit
CACAAGGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG28750.43044334916366733No Hit
CACAAGGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG27420.41053066553279155No Hit
CACAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC23650.35408644200767764No Hit
CACAAGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC23330.34929542038220374No Hit
CACAAGGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCT22160.33177824756406493No Hit
CACAAGGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT18890.2828199953287539No Hit
CACAAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC17180.257217973517628No Hit
CACAAGGGGGAAGATACTTTATGAACTTTACAGTTAAAAGATTAAAAATCAT15400.2305679157259296No Hit
CACAAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCT15130.22652549122943602No Hit
CACAAGGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC14840.22218362788135035No Hit
CACAAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC13060.195533570089652No Hit
CACAAGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTCT11590.17352481449763144No Hit
CACAAGGGGGGGGGCTGGTGAGATGGCTCAGTGGTTAAGAGCTCTGACTACT11260.16858407344636153No Hit
CACAAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC10500.1572053970858611No Hit
CACAAGGGGCTCTTCATCTCTAACCATGCCTACTAACCAGAGCTGAACTAAG10450.1564567999568808No Hit
CACAAGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGG10230.15316297258936754No Hit
CACAAGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTCT9770.14627587900274885No Hit
CACAAGGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCG9730.14567700129956462No Hit
CACAAGGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG9220.13804131058396565No Hit
CACAAGGGGGGCCTCTCCTTCCTGCGGCGCCTTAGGGACCATGGCCGATCCT8600.12875870618461005No Hit
CACAAGGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCAACGACTGCTC8360.12516543996550467No Hit
CACAAGGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTG7720.11558339671455692No Hit
CACAAGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGA7620.11408620245659633No Hit
CACAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAACTGCTC6690.10016229585756292No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGACC206.309546E-446.013
AACGTAA301.8602241E-646.043
GATACGC301.8602241E-646.032
CGAACGC206.309546E-446.045
CATATCG206.309546E-446.033
AATCACG206.309546E-446.014
ACGCATC253.4156074E-546.023
ACGTTGC206.309546E-446.017
ATAGCCG253.4156074E-546.039
GTAGGTA453.092282E-1046.029
TACGGTG253.4156074E-546.035
TAAGTCC253.4156074E-546.030
TCGATAT206.309546E-446.038
TTATCCG206.309546E-446.025
CGTTAGT253.4156074E-546.019
TTATACG253.4156074E-546.022
ACGATTA253.4156074E-546.032
CTAACTT206.309546E-446.022
TACGCTC301.8602241E-646.034
ATATCGC206.309546E-446.034
Produced by FastQC (version 0.11.5)