##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527590_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	667916
Sequences flagged as poor quality	0
Sequence length	52
%GC	56
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.163099850879455	33.0	31.0	34.0	31.0	34.0
2	31.811221770402266	31.0	31.0	34.0	30.0	34.0
3	32.6834302517083	34.0	31.0	34.0	31.0	34.0
4	36.02578917109337	37.0	35.0	37.0	35.0	37.0
5	36.21375142982052	37.0	35.0	37.0	35.0	37.0
6	36.37052413776583	37.0	37.0	37.0	35.0	37.0
7	36.6813611292438	37.0	37.0	37.0	35.0	37.0
8	36.75499164565604	37.0	37.0	37.0	35.0	37.0
9	38.74830218171147	39.0	39.0	39.0	39.0	39.0
10	37.713142071757524	39.0	37.0	39.0	35.0	39.0
11	36.687060947783856	37.0	35.0	39.0	34.0	39.0
12	36.563314847974894	39.0	35.0	39.0	33.0	39.0
13	36.74092700279676	39.0	35.0	39.0	33.0	39.0
14	37.423593685433495	40.0	35.0	40.0	33.0	40.0
15	37.58880607741093	40.0	35.0	40.0	34.0	40.0
16	37.71656465783123	40.0	35.0	40.0	34.0	40.0
17	37.608444774492604	40.0	35.0	40.0	34.0	40.0
18	37.53158481006594	40.0	35.0	40.0	34.0	40.0
19	37.378734751076486	40.0	35.0	40.0	33.0	40.0
20	37.316134064762636	40.0	35.0	40.0	33.0	40.0
21	37.277596284562726	40.0	35.0	40.0	33.0	40.0
22	37.331366519143124	39.0	35.0	40.0	33.0	40.0
23	37.25513088472203	39.0	35.0	40.0	33.0	40.0
24	37.235378400876755	39.0	35.0	40.0	33.0	40.0
25	37.26469645883614	39.0	35.0	40.0	34.0	40.0
26	37.22241569299133	39.0	35.0	40.0	34.0	40.0
27	37.22022529779194	39.0	35.0	40.0	33.0	40.0
28	37.08845723114883	39.0	35.0	40.0	33.0	40.0
29	37.05006168440343	39.0	35.0	40.0	33.0	40.0
30	36.872398624976796	39.0	35.0	40.0	33.0	40.0
31	36.82244174417142	39.0	35.0	40.0	33.0	40.0
32	36.74265925655322	39.0	35.0	40.0	33.0	40.0
33	36.61755669874655	38.0	35.0	40.0	33.0	40.0
34	36.177469921367354	38.0	35.0	40.0	31.0	40.0
35	36.141761838315	38.0	35.0	40.0	31.0	40.0
36	36.125827199827526	38.0	35.0	40.0	31.0	40.0
37	36.09534282754119	38.0	35.0	40.0	31.0	40.0
38	36.21611849394235	38.0	35.0	40.0	31.0	40.0
39	36.185292761365204	38.0	35.0	40.0	32.0	40.0
40	35.970563663694236	37.0	35.0	40.0	31.0	40.0
41	35.87914498230317	37.0	35.0	40.0	31.0	40.0
42	35.748959449990714	37.0	35.0	40.0	31.0	40.0
43	35.75689907114068	37.0	35.0	40.0	31.0	40.0
44	35.63901298965738	36.0	35.0	40.0	31.0	40.0
45	35.55399181933058	36.0	34.0	40.0	31.0	40.0
46	35.37832152546129	36.0	34.0	40.0	30.0	40.0
47	35.239673851202845	35.0	34.0	40.0	30.0	40.0
48	35.091998095568904	35.0	34.0	40.0	30.0	40.0
49	34.94472658238461	35.0	34.0	39.0	29.0	40.0
50	35.336724977392365	35.0	34.0	40.0	31.0	40.0
51	35.43874828571258	35.0	35.0	40.0	31.0	40.0
52	35.19239545092497	35.0	34.0	39.0	31.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	5.0
14	6.0
15	14.0
16	29.0
17	72.0
18	135.0
19	248.0
20	337.0
21	571.0
22	783.0
23	1320.0
24	1742.0
25	2277.0
26	3003.0
27	3980.0
28	5097.0
29	7093.0
30	9603.0
31	13670.0
32	19837.0
33	36415.0
34	90029.0
35	54051.0
36	58223.0
37	100486.0
38	204165.0
39	54724.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	0.0	100.0
2	0.0	100.0	0.0	0.0
3	0.0	0.0	0.0	100.0
4	0.0	100.0	0.0	0.0
5	0.0	100.0	0.0	0.0
6	100.0	0.0	0.0	0.0
7	98.79146479497422	1.103881326394337	0.036830978745830315	0.06782289988561435
8	99.46130950598578	0.39675647835955413	0.0988148210253984	0.04311919462926476
9	97.86844453494152	1.191167751633439	0.39406152869522515	0.5463261847298163
10	58.911899101084565	30.90583246995131	3.476035908707083	6.706232520257038
11	39.64121236802233	24.69472209080184	20.342378382910425	15.32168715826541
12	32.951448984602855	17.40054138544368	25.284766347864103	24.363243282089364
13	25.885141245306297	16.913803532180694	28.1487492439169	29.05230597859611
14	24.445588966277196	18.818534067158147	29.15336659100845	27.582510375556208
15	26.229945082914618	19.82375029195288	27.079003946604065	26.867300678528437
16	32.7141436947161	22.031812383593145	22.77307326070943	22.48097066098132
17	32.07888417106343	21.784625611603854	22.64236820198947	23.494122015343248
18	32.68869139233077	21.539984069853098	21.803490259254158	23.967834278561977
19	30.11890716796723	23.78308050712964	20.87927224381509	25.218740081088043
20	31.620892447553285	22.544301978093053	22.47108917887878	23.363716395474878
21	30.85268207379371	23.108744213344192	24.045987818827516	21.99258589403458
22	30.667628863509783	20.199695770126784	25.519077249234932	23.6135981171285
23	30.38256307679409	20.52683271549117	24.306200180861065	24.784404026853675
24	30.021739260625584	20.830463711005574	24.257541367477348	24.890255660891487
25	28.95513807125447	22.278699716730845	22.03585480808964	26.730307403925046
26	25.942184346534596	22.99974847136466	24.43181477910396	26.626252402996787
27	25.893525533150875	20.628043047329307	24.05257547356254	29.425855945957274
28	27.87491241413591	23.336168021128405	22.50986651015996	26.279053054575723
29	30.471945573994336	21.46871762317417	21.872960072823528	26.186376730007964
30	27.70198647734146	21.22811850591991	23.935195443738433	27.134699573000198
31	32.037112451266324	22.116553578593713	21.940782972709144	23.905550997430815
32	33.604674839351055	21.00084441756149	21.99019038322184	23.404290359865612
33	33.201031267404886	19.90355074590218	23.167434228256248	23.72798375843669
34	29.11997915905593	22.08481306032495	27.25058240856635	21.54462537205277
35	27.21494918522688	26.24087460099773	24.673162493487204	21.87101372028818
36	28.28903634588781	27.03768138508435	22.141706442127454	22.531575826900387
37	31.369962689919088	25.804891633079606	20.881817474053623	21.943328202947676
38	30.559681157510827	26.46395654543386	20.334742692194826	22.64161960486049
39	31.821067319842616	22.3032537025614	21.427245342228662	24.448433635367323
40	28.743734242030435	22.982530737398115	21.31420717575264	26.95952784481881
41	26.223956305882773	24.887111552949772	21.447008306433744	27.44192383473371
42	25.069320094143578	21.589391480365794	23.96813371741357	29.373154708077067
43	25.587948185101123	20.596003090208946	25.950418914953378	27.865629809736554
44	26.350169781828853	19.836177004293955	24.60474071589841	29.20891249797879
45	28.247713784368095	20.412596793608778	24.32132184286647	27.01836757915666
46	27.553315087525977	23.523317303373478	22.711388857281452	26.211978751819093
47	25.57701866701801	22.39667862425814	25.484192623024455	26.5421100856994
48	28.402972828918603	21.627120775666402	24.813000437180722	25.156905958234272
49	27.944981105408463	21.0992100803095	25.503805867803734	25.4520029464783
50	25.268746369303923	21.798998676480277	25.180561627510045	27.75169332670575
51	24.385701195958774	22.587271453296523	24.569856089687924	28.45717126105678
52	25.245240419453946	21.81771360470478	25.29434839111505	27.64269758472622
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.5
6	1.0
7	2.0
8	3.0
9	3.0
10	3.0
11	36.0
12	69.0
13	163.5
14	441.5
15	625.0
16	619.0
17	613.0
18	558.5
19	504.0
20	496.5
21	489.0
22	579.0
23	669.0
24	890.0
25	1111.0
26	1614.5
27	2118.0
28	3183.0
29	4248.0
30	4843.5
31	5439.0
32	6256.0
33	7073.0
34	6903.0
35	6733.0
36	8868.0
37	11003.0
38	10990.5
39	12602.0
40	14226.0
41	16385.0
42	18544.0
43	19164.5
44	19785.0
45	20718.5
46	21652.0
47	22198.5
48	22745.0
49	25221.5
50	27698.0
51	28667.5
52	29637.0
53	32513.5
54	35390.0
55	38213.0
56	41036.0
57	46830.0
58	52624.0
59	58395.5
60	64167.0
61	61613.0
62	59059.0
63	59899.5
64	53922.0
65	47104.0
66	40866.5
67	34629.0
68	26825.0
69	19021.0
70	17370.0
71	15719.0
72	13919.5
73	12120.0
74	11308.5
75	10497.0
76	8502.5
77	6508.0
78	3961.0
79	1414.0
80	1099.5
81	785.0
82	678.0
83	571.0
84	408.0
85	245.0
86	150.0
87	55.0
88	31.5
89	4.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	667916.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	9.640882985285575
#Duplication Level	Percentage of deduplicated	Percentage of total
1	65.60651002438154	6.325046862180274
2	9.538303852903265	1.8391534264787786
3	3.233270697125464	0.9351475335221795
4	1.4597860015840232	0.5629450409931788
5	0.931778298883419	0.44915827738817454
6	0.6662214837016446	0.3853778019990538
7	0.569937726150358	0.38462920487007346
8	0.4457006196325688	0.3437558016277496
9	0.43482987281226215	0.37729295300606663
>10	12.478064385880453	32.322777115685206
>50	3.0515739288431973	19.91942100503656
>100	1.5141397356855557	27.341462100024554
>500	0.04037705961828149	2.5667898358476213
>1k	0.02795334896650257	5.115763060025512
>5k	0.0015529638314723648	1.1312799813150156
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CACAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	7556	1.1312799813150156	No Hit
CACAAGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA	3426	0.5129387527772954	No Hit
CACAAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	3359	0.5029075512489595	No Hit
CACAAGGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG	2875	0.43044334916366733	No Hit
CACAAGGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG	2742	0.41053066553279155	No Hit
CACAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC	2365	0.35408644200767764	No Hit
CACAAGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	2333	0.34929542038220374	No Hit
CACAAGGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCT	2216	0.33177824756406493	No Hit
CACAAGGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT	1889	0.2828199953287539	No Hit
CACAAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC	1718	0.257217973517628	No Hit
CACAAGGGGGAAGATACTTTATGAACTTTACAGTTAAAAGATTAAAAATCAT	1540	0.2305679157259296	No Hit
CACAAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCT	1513	0.22652549122943602	No Hit
CACAAGGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC	1484	0.22218362788135035	No Hit
CACAAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC	1306	0.195533570089652	No Hit
CACAAGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTCT	1159	0.17352481449763144	No Hit
CACAAGGGGGGGGGCTGGTGAGATGGCTCAGTGGTTAAGAGCTCTGACTACT	1126	0.16858407344636153	No Hit
CACAAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC	1050	0.1572053970858611	No Hit
CACAAGGGGCTCTTCATCTCTAACCATGCCTACTAACCAGAGCTGAACTAAG	1045	0.1564567999568808	No Hit
CACAAGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGG	1023	0.15316297258936754	No Hit
CACAAGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTCT	977	0.14627587900274885	No Hit
CACAAGGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCG	973	0.14567700129956462	No Hit
CACAAGGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG	922	0.13804131058396565	No Hit
CACAAGGGGGGCCTCTCCTTCCTGCGGCGCCTTAGGGACCATGGCCGATCCT	860	0.12875870618461005	No Hit
CACAAGGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCAACGACTGCTC	836	0.12516543996550467	No Hit
CACAAGGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTG	772	0.11558339671455692	No Hit
CACAAGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGA	762	0.11408620245659633	No Hit
CACAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAACTGCTC	669	0.10016229585756292	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.06003748974421933	0.0	0.0	0.0	0.0
6	0.06003748974421933	0.0	0.0	0.0	0.0
7	0.06063636744740357	0.0	0.0	0.0	0.0
8	0.060935806298995684	0.0	0.0	0.0	0.0
9	0.06168440342797597	0.0	0.0	0.0	0.0
10	0.07695578485917391	0.0	0.0	0.0	0.0
11	0.08923277777445068	0.0	0.0	0.0	0.0
12	0.09611987136106935	0.0	0.0	0.0	0.0
13	0.10330640379928015	0.0	0.0	0.0	0.0
14	0.11109181394067517	0.0	0.0	0.0	0.0
15	0.11857778523047809	0.0	0.0	0.0	0.0
16	0.12801010905562976	0.0	0.0	0.0	0.0
17	0.13639439690020902	0.0	0.0	0.0	0.0
18	0.14298205163523556	0.0	0.0	0.0	0.0
19	0.15271381431197936	0.0	0.0	0.0	0.0
20	0.16229585756292708	0.0	0.0	0.0	0.0
21	0.16708687918840093	0.0	0.0	0.0	0.0
22	0.17217733966546692	0.0	0.0	0.0	0.0
23	0.17756723899412502	0.0	0.0	0.0	0.0
24	0.18445433258074367	0.0	0.0	0.0	0.0
25	0.1911917067415663	0.0	0.0	0.0	0.0
26	0.19747992262500075	0.0	0.0	0.0	0.0
27	0.2040675773600273	0.0	0.0	0.0	0.0
28	0.210954670946646	0.0	0.0	0.0	0.0
29	0.21679372855269224	0.0	0.0	0.0	0.0
30	0.2233813832877188	0.0	0.0	0.0	0.0
31	0.23206510998389018	0.0	0.0	0.0	0.0
32	0.24224603093802213	0.0	0.0	0.0	0.0
33	0.2501811605052132	0.0	0.0	0.0	0.0
34	0.2567688152402398	0.0	0.0	0.0	0.0
35	0.2656022613622072	0.0	0.0	0.0	0.0
36	0.2754837434647471	0.0	0.0	0.0	0.0
37	0.2867127003994514	0.0	0.0	0.0	0.0
38	0.29374951341186617	0.0	0.0	0.0	0.0
39	0.3018343624048533	0.0	0.0	0.0	0.0
40	0.31036836967522863	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTTGACC	20	6.309546E-4	46.0	13
AACGTAA	30	1.8602241E-6	46.0	43
GATACGC	30	1.8602241E-6	46.0	32
CGAACGC	20	6.309546E-4	46.0	45
CATATCG	20	6.309546E-4	46.0	33
AATCACG	20	6.309546E-4	46.0	14
ACGCATC	25	3.4156074E-5	46.0	23
ACGTTGC	20	6.309546E-4	46.0	17
ATAGCCG	25	3.4156074E-5	46.0	39
GTAGGTA	45	3.092282E-10	46.0	29
TACGGTG	25	3.4156074E-5	46.0	35
TAAGTCC	25	3.4156074E-5	46.0	30
TCGATAT	20	6.309546E-4	46.0	38
TTATCCG	20	6.309546E-4	46.0	25
CGTTAGT	25	3.4156074E-5	46.0	19
TTATACG	25	3.4156074E-5	46.0	22
ACGATTA	25	3.4156074E-5	46.0	32
CTAACTT	20	6.309546E-4	46.0	22
TACGCTC	30	1.8602241E-6	46.0	34
ATATCGC	20	6.309546E-4	46.0	34
>>END_MODULE