FastQCFastQC Report
Fri 17 Jun 2016
SRR1527589_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527589_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences789185
Sequences flagged as poor quality0
Sequence length52
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC82921.050704207505211No Hit
CACAAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT38790.49151973238214103No Hit
CACAAGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA34730.44007425381881304No Hit
CACAAGGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG34690.43956740181326304No Hit
CACAAGGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCT28830.36531358300018374No Hit
CACAAGGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG28710.36379302698353366No Hit
CACAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC27870.353149134866983No Hit
CACAAGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC26150.3313544986283318No Hit
CACAAGGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT24820.3145016694437933No Hit
CACAAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC21190.2685048499401281No Hit
CACAAGGGGGAAGATACTTTATGAACTTTACAGTTAAAAGATTAAAAATCAT19460.24658350070008933No Hit
CACAAGGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC19100.24202183265013907No Hit
CACAAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCT17300.21921349240038773No Hit
CACAAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC16660.2111038603115873No Hit
CACAAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC14420.18272014800078562No Hit
CACAAGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTCT14070.17828519295222287No Hit
CACAAGGGGGGGGGCTGGTGAGATGGCTCAGTGGTTAAGAGCTCTGACTACT12530.15877139073854674No Hit
CACAAGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGG11830.14990148064142123No Hit
CACAAGGGGCTCTTCATCTCTAACCATGCCTACTAACCAGAGCTGAACTAAG11830.14990148064142123No Hit
CACAAGGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCG11430.1448329605859209No Hit
CACAAGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTCT11340.14369254357343336No Hit
CACAAGGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG11000.1393843015262581No Hit
CACAAGGGGGGCCTCTCCTTCCTGCGGCGCCTTAGGGACCATGGCCGATCCT10160.1287404094097075No Hit
CACAAGGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCAACGACTGCTC9550.12101091632506954No Hit
CACAAGGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTG9370.1187300823000944No Hit
CACAAGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGA8650.10960674620019387No Hit
CACAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAACTGCTC8370.10605878216134365No Hit
CACAAGGGGGAGACCGACCCTCGGCTCCGGAGCGGCAGTGCCGTCCCTCTCG8130.10301767012804348No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGGTA700.046.00000429
TCGTTTG206.3102454E-446.035
TCGTTTA206.3102454E-446.028
AATTTCG206.3102454E-446.022
CGTATCA301.860657E-646.043
ATAGGTG206.3102454E-446.031
ATAGGCC253.416176E-546.011
GCGAACC206.3102454E-446.026
GACGTTA253.416176E-546.010
GCTATAA206.3102454E-446.022
ATAGCCG301.860657E-646.039
TGTATCG206.3102454E-446.026
ATATGTC750.046.046
TACGGTG206.3102454E-446.035
CGATTAC301.860657E-646.010
GCGTTAG206.3102454E-446.025
TTATCGC206.3102454E-446.034
CGTTCAC206.3102454E-446.044
TATGATC206.3102454E-446.041
CGTTAGT301.860657E-646.019
Produced by FastQC (version 0.11.5)