##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527589_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	789185
Sequences flagged as poor quality	0
Sequence length	52
%GC	56
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.133337557100045	31.0	31.0	33.0	30.0	34.0
2	30.643423278445486	31.0	31.0	33.0	28.0	34.0
3	31.898343227506857	31.0	31.0	34.0	30.0	34.0
4	35.263347630783656	35.0	35.0	37.0	33.0	37.0
5	35.58611605643797	37.0	35.0	37.0	35.0	37.0
6	35.8325297617162	37.0	35.0	37.0	35.0	37.0
7	36.46553976570766	37.0	37.0	37.0	35.0	37.0
8	36.60635085562954	37.0	37.0	37.0	35.0	37.0
9	38.64591699031279	39.0	39.0	39.0	38.0	39.0
10	37.10797975126238	39.0	37.0	39.0	34.0	39.0
11	35.988622439605415	37.0	35.0	39.0	32.0	39.0
12	35.82381444148077	38.0	35.0	39.0	31.0	39.0
13	35.99331082065675	38.0	35.0	39.0	32.0	39.0
14	36.75178570297205	39.0	35.0	40.0	32.0	40.0
15	36.95933272933469	39.0	35.0	40.0	33.0	40.0
16	37.04880731387444	39.0	35.0	40.0	33.0	40.0
17	36.96105475902355	39.0	35.0	40.0	33.0	40.0
18	36.93057394653979	39.0	35.0	40.0	33.0	40.0
19	36.744759467045114	39.0	35.0	40.0	32.0	40.0
20	36.62757401623193	39.0	35.0	40.0	31.0	40.0
21	36.64887954028523	39.0	35.0	40.0	31.0	40.0
22	36.700212244277324	39.0	35.0	40.0	32.0	40.0
23	36.683593834145356	38.0	35.0	40.0	32.0	40.0
24	36.68806553596432	38.0	35.0	40.0	32.0	40.0
25	36.703542261953785	38.0	35.0	40.0	32.0	40.0
26	36.615929091404425	38.0	35.0	40.0	32.0	40.0
27	36.57730190006146	38.0	35.0	40.0	32.0	40.0
28	36.3888771327382	38.0	35.0	40.0	31.0	40.0
29	36.37637055950126	38.0	35.0	40.0	31.0	40.0
30	36.2520727079202	38.0	35.0	40.0	31.0	40.0
31	36.2014977476764	38.0	35.0	40.0	31.0	40.0
32	36.118382888676294	38.0	35.0	40.0	31.0	40.0
33	35.99153810576734	38.0	35.0	40.0	31.0	40.0
34	35.582046034833404	38.0	34.0	40.0	30.0	40.0
35	35.523766924105246	37.0	34.0	40.0	30.0	40.0
36	35.580208696313285	37.0	34.0	40.0	30.0	40.0
37	35.62990680258748	37.0	34.0	40.0	30.0	40.0
38	35.5749957234362	37.0	34.0	40.0	30.0	40.0
39	35.48217084713977	37.0	34.0	40.0	30.0	40.0
40	35.652033426889766	37.0	34.0	40.0	30.0	40.0
41	35.75272971483239	37.0	34.0	40.0	31.0	40.0
42	35.77940533588449	37.0	35.0	40.0	31.0	40.0
43	35.76479279256448	36.0	35.0	40.0	31.0	40.0
44	35.661483682533245	36.0	34.0	40.0	31.0	40.0
45	35.60695654377617	36.0	34.0	40.0	31.0	40.0
46	35.37593973529654	35.0	34.0	40.0	31.0	40.0
47	35.29603578375159	35.0	34.0	40.0	30.0	40.0
48	35.230327489752085	35.0	34.0	40.0	30.0	40.0
49	35.07617098652407	35.0	34.0	39.0	30.0	40.0
50	34.986108453657884	35.0	34.0	39.0	30.0	40.0
51	34.88181098221583	35.0	34.0	39.0	30.0	40.0
52	34.517729049589136	35.0	34.0	39.0	29.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	2.0
13	1.0
14	10.0
15	21.0
16	36.0
17	123.0
18	195.0
19	399.0
20	567.0
21	878.0
22	1431.0
23	2153.0
24	3007.0
25	3962.0
26	5405.0
27	6904.0
28	8949.0
29	11904.0
30	16033.0
31	22057.0
32	31459.0
33	52086.0
34	107169.0
35	61724.0
36	79610.0
37	127838.0
38	214766.0
39	30496.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	0.0	100.0
2	0.0	100.0	0.0	0.0
3	0.0	0.0	0.0	100.0
4	0.0	100.0	0.0	0.0
5	0.0	100.0	0.0	0.0
6	100.0	0.0	0.0	0.0
7	98.83930890729043	1.0804817628312753	0.02660973029137655	0.05359959958691562
8	99.47236706222242	0.4222077206231746	0.07881548686302958	0.02660973029137655
9	97.96790359674854	1.1527081736221545	0.38216641218472225	0.4972218174445789
10	58.66153056634376	31.087007482402733	3.4393709966611126	6.812090954592397
11	39.11909121435405	24.997180635719126	20.48898547235439	15.394742677572431
12	32.44055576322409	17.49830521360644	25.413686271279868	24.647452751889606
13	25.506820327299685	16.956353706672072	28.343797715364584	29.193028250663662
14	24.243491703466233	18.87162072264425	29.081394096441265	27.803493477448253
15	26.083111057610065	19.78737558367176	27.023448240906756	27.10606511781141
16	32.38302806059416	22.07302470269962	22.76753866330455	22.776408573401675
17	31.8662924409359	21.992435233817165	22.464694589988408	23.676577735258526
18	32.30877424178108	21.527651944727786	21.825300784987043	24.33827302850409
19	29.85725780393697	23.70090663152493	20.935522089243968	25.506313475294135
20	31.387697434695287	22.361423493857586	22.652229832041918	23.59864923940521
21	30.677217635915532	23.0195708230643	24.059884564455736	22.243326976564433
22	30.67747106191831	20.11454855325431	25.531149223566086	23.6768311612613
23	30.349791240330216	20.6288766258862	24.222710771238685	24.798621362544903
24	29.788959496189104	20.785113756596996	24.31533797525295	25.110588771960945
25	28.65817267180699	22.3856256771226	22.01004834101003	26.946153310060378
26	25.91724373879382	23.011334477974113	24.327375710384764	26.744046072847304
27	25.783941661334158	20.96124482852563	23.828126484917984	29.426687025222225
28	28.041333781052607	23.314178551290254	22.468242554027256	26.176245113629882
29	30.382863333692356	21.51688133960985	21.9160272939805	26.1842280327173
30	27.65219815379157	21.361531199908768	23.852455381184388	27.13381526511528
31	31.835627894600126	22.380177018062938	21.697574079588435	24.086621007748498
32	33.38380734555269	21.190468648035633	21.87991408858506	23.545809917826617
33	32.975411342080754	19.95717100553102	23.29213048904883	23.775287163339396
34	29.169079493401423	22.080374056780098	27.196284774799317	21.554261675019166
35	27.168027775489907	26.36111938265426	24.607411443451156	21.863441398404685
36	28.179957804570538	27.22048695806433	22.060986967567807	22.538568269797324
37	31.107281562624735	25.774691612232875	20.955035891457644	22.16299093368475
38	30.564443064680653	26.38633526993037	20.390782896279074	22.658438769109903
39	31.647459087539676	22.268036011834994	21.612042803651867	24.47246209697346
40	28.53665490347637	23.09433149388293	21.461887897007674	26.907125705633028
41	26.159012145441185	24.79431312049773	21.464548870036808	27.582125864024277
42	24.71486406862776	21.737488675025503	23.981575929598257	29.56607132674848
43	25.532162927577183	20.554242668068955	26.053840354289555	27.859754050064307
44	26.192844516811647	19.986695134854312	24.690915311365522	29.12954503696852
45	28.037278965008205	20.630523894904236	24.248560223521736	27.083636916565823
46	27.655365978826257	23.58344367923871	22.562390314058174	26.19880002787686
47	25.578920025089175	22.430862218617943	25.45486799673081	26.53534975956208
48	28.298054321863695	21.73089959895335	24.694970127409924	25.27607595177303
49	27.726958824610197	21.16575961276507	25.611992118451315	25.49528944417342
50	25.1952330568878	21.960376844466126	25.040516482193652	27.803873616452417
51	24.404670641231142	22.55453410797215	24.41240013431578	28.628395116480927
52	25.13479095522596	21.935667809195564	25.244904553431706	27.684636682146774
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	1.5
6	3.0
7	4.0
8	5.0
9	3.5
10	2.0
11	50.0
12	98.0
13	207.5
14	512.0
15	707.0
16	721.0
17	735.0
18	633.0
19	531.0
20	512.5
21	494.0
22	640.0
23	786.0
24	971.0
25	1156.0
26	1835.0
27	2514.0
28	3838.5
29	5163.0
30	5793.0
31	6423.0
32	7267.5
33	8112.0
34	8040.0
35	7968.0
36	10708.0
37	13448.0
38	13116.5
39	15130.5
40	17476.0
41	19889.5
42	22303.0
43	23131.5
44	23960.0
45	24753.5
46	25547.0
47	26328.0
48	27109.0
49	29846.5
50	32584.0
51	33957.0
52	35330.0
53	38993.0
54	42656.0
55	45887.0
56	49118.0
57	55112.5
58	61107.0
59	68183.0
60	75259.0
61	72185.5
62	69112.0
63	70171.0
64	63474.0
65	55718.0
66	48087.5
67	40457.0
68	31231.5
69	22006.0
70	20099.0
71	18192.0
72	16346.5
73	14501.0
74	13656.0
75	12811.0
76	10225.0
77	7639.0
78	4676.5
79	1714.0
80	1383.0
81	1052.0
82	902.0
83	752.0
84	490.5
85	229.0
86	138.5
87	48.0
88	37.0
89	13.0
90	0.0
91	0.0
92	0.0
93	1.0
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	789185.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	9.326330328123317
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.5321594521888	6.205008964944849
2	9.980707046004184	1.861667416385258
3	3.7268009021493977	1.042721288417798
4	1.627673161055406	0.6072087026489352
5	0.904866715578381	0.4219542946203995
6	0.586940572267058	0.32844009959641907
7	0.43205347680769546	0.2820631410885914
8	0.35732724654221354	0.2666041549193155
9	0.3940110323089046	0.3307209336213942
>10	10.176353903426536	26.614925524433435
>50	3.5053395288171516	22.201511686106553
>100	1.69424743892829	30.154146366187902
>500	0.05027037308768783	2.969392474514848
>1k	0.029890492106192764	5.662930745009092
>5k	0.0013586587320996713	1.050704207505211
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CACAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	8292	1.050704207505211	No Hit
CACAAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	3879	0.49151973238214103	No Hit
CACAAGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA	3473	0.44007425381881304	No Hit
CACAAGGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG	3469	0.43956740181326304	No Hit
CACAAGGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCT	2883	0.36531358300018374	No Hit
CACAAGGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG	2871	0.36379302698353366	No Hit
CACAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC	2787	0.353149134866983	No Hit
CACAAGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	2615	0.3313544986283318	No Hit
CACAAGGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT	2482	0.3145016694437933	No Hit
CACAAGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC	2119	0.2685048499401281	No Hit
CACAAGGGGGAAGATACTTTATGAACTTTACAGTTAAAAGATTAAAAATCAT	1946	0.24658350070008933	No Hit
CACAAGGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC	1910	0.24202183265013907	No Hit
CACAAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCT	1730	0.21921349240038773	No Hit
CACAAGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC	1666	0.2111038603115873	No Hit
CACAAGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC	1442	0.18272014800078562	No Hit
CACAAGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTCT	1407	0.17828519295222287	No Hit
CACAAGGGGGGGGGCTGGTGAGATGGCTCAGTGGTTAAGAGCTCTGACTACT	1253	0.15877139073854674	No Hit
CACAAGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGG	1183	0.14990148064142123	No Hit
CACAAGGGGCTCTTCATCTCTAACCATGCCTACTAACCAGAGCTGAACTAAG	1183	0.14990148064142123	No Hit
CACAAGGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCG	1143	0.1448329605859209	No Hit
CACAAGGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTCT	1134	0.14369254357343336	No Hit
CACAAGGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG	1100	0.1393843015262581	No Hit
CACAAGGGGGGCCTCTCCTTCCTGCGGCGCCTTAGGGACCATGGCCGATCCT	1016	0.1287404094097075	No Hit
CACAAGGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCAACGACTGCTC	955	0.12101091632506954	No Hit
CACAAGGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTG	937	0.1187300823000944	No Hit
CACAAGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGA	865	0.10960674620019387	No Hit
CACAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAACTGCTC	837	0.10605878216134365	No Hit
CACAAGGGGGAGACCGACCCTCGGCTCCGGAGCGGCAGTGCCGTCCCTCTCG	813	0.10301767012804348	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.04637695850782769	0.0	0.0	0.0	0.0
6	0.04637695850782769	0.0	0.0	0.0	0.0
7	0.04675709751199022	0.0	0.0	0.0	0.0
8	0.04713723651615274	0.0	0.0	0.0	0.0
9	0.04789751452447778	0.0	0.0	0.0	0.0
10	0.060188675659066	0.0	0.0	0.0	0.0
11	0.068931872754804	0.0	0.0	0.0	0.0
12	0.07336682780336676	0.0	0.0	0.0	0.0
13	0.07995590387551715	0.0	0.0	0.0	0.0
14	0.08641826694628002	0.0	0.0	0.0	0.0
15	0.09364090802536794	0.0	0.0	0.0	0.0
16	0.10124368810861839	0.0	0.0	0.0	0.0
17	0.10884646819186883	0.0	0.0	0.0	0.0
18	0.11505540525985669	0.0	0.0	0.0	0.0
19	0.12443216736253224	0.0	0.0	0.0	0.0
20	0.13165480844162014	0.0	0.0	0.0	0.0
21	0.13811717151238304	0.0	0.0	0.0	0.0
22	0.1425521265609458	0.0	0.0	0.0	0.0
23	0.14800078562060862	0.0	0.0	0.0	0.0
24	0.15294259267472138	0.0	0.0	0.0	0.0
25	0.15927824274409677	0.0	0.0	0.0	0.0
26	0.16776801383705975	0.0	0.0	0.0	0.0
27	0.17461051591198515	0.0	0.0	0.0	0.0
28	0.18157973098829805	0.0	0.0	0.0	0.0
29	0.18905579807016099	0.0	0.0	0.0	0.0
30	0.19501130913537384	0.0	0.0	0.0	0.0
31	0.2043880712380494	0.0	0.0	0.0	0.0
32	0.2131312683337874	0.0	0.0	0.0	0.0
33	0.22060733541565034	0.0	0.0	0.0	0.0
34	0.2270696984864132	0.0	0.0	0.0	0.0
35	0.2374601646001888	0.0	0.0	0.0	0.0
36	0.2496246127333895	0.0	0.0	0.0	0.0
37	0.2578609578235775	0.0	0.0	0.0	0.0
38	0.266984293923478	0.0	0.0	0.0	0.0
39	0.27319323099146586	0.0	0.0	0.0	0.0
40	0.28244328009275393	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTAGGTA	70	0.0	46.000004	29
TCGTTTG	20	6.3102454E-4	46.0	35
TCGTTTA	20	6.3102454E-4	46.0	28
AATTTCG	20	6.3102454E-4	46.0	22
CGTATCA	30	1.860657E-6	46.0	43
ATAGGTG	20	6.3102454E-4	46.0	31
ATAGGCC	25	3.416176E-5	46.0	11
GCGAACC	20	6.3102454E-4	46.0	26
GACGTTA	25	3.416176E-5	46.0	10
GCTATAA	20	6.3102454E-4	46.0	22
ATAGCCG	30	1.860657E-6	46.0	39
TGTATCG	20	6.3102454E-4	46.0	26
ATATGTC	75	0.0	46.0	46
TACGGTG	20	6.3102454E-4	46.0	35
CGATTAC	30	1.860657E-6	46.0	10
GCGTTAG	20	6.3102454E-4	46.0	25
TTATCGC	20	6.3102454E-4	46.0	34
CGTTCAC	20	6.3102454E-4	46.0	44
TATGATC	20	6.3102454E-4	46.0	41
CGTTAGT	30	1.860657E-6	46.0	19
>>END_MODULE