SRR1527588_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR1527588_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	765828
Sequences flagged as poor quality	0
Sequence length	50
%GC	57

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
AGGACTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC	8778	1.146210376220248	No Hit
AGGACTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTAGGACTGGGA	7768	1.0143269768146372	No Hit
AGGACTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT	5639	0.7363272170774638	No Hit
AGGACTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	4721	0.6164569590038494	No Hit
AGGACTGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCA	3897	0.5088609975085789	No Hit
AGGACTGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC	2576	0.33636795729589414	No Hit
AGGACTGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	2533	0.3307531194994176	No Hit
AGGACTGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCC	2326	0.30372355150242614	No Hit
AGGACTGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTT	2075	0.27094856808578427	No Hit
AGGACTGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGA	2040	0.2663783512746988	No Hit
AGGACTGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGC	1858	0.24261322385705406	No Hit
AGGACTGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAG	1842	0.2405239818862721	No Hit
AGGACTGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTG	1803	0.2354314545824911	No Hit
AGGACTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG	1553	0.2027870487890231	No Hit
AGGACTGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTC	1509	0.19704163336937275	No Hit
AGGACTGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCAT	1415	0.1847673367910288	No Hit
AGGACTGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCG	1373	0.17928307661772616	No Hit
AGGACTGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGC	1311	0.1711872639809461	No Hit
AGGACTGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCA	1224	0.15982701076481926	No Hit
AGGACTGGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTG	1157	0.15107831001216984	No Hit
AGGACTGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCC	1096	0.14311307499856363	No Hit
AGGACTGGGCTCTTTTTCGTGACGCCTCCCAGGCGCTCGGCTGTGTCAAG	1064	0.13893459105699973	No Hit
AGGACTGGGCTCTTTCTTGACTCCATCTTCGCGGTAGCAGCAGCGGCGTC	1060	0.13841228056430424	No Hit
AGGACTGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGC	1045	0.13645361621669616	No Hit
AGGACTGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTT	1033	0.1348866847386097	No Hit
AGGACTGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCG	969	0.1265297168554819	No Hit
AGGACTGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGC	940	0.12274296578343963	No Hit
AGGACTGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTT	935	0.12209007766757027	No Hit
AGGACTGGGGCCTCTCCTTCCTGCGGCGCCTTAGGGACCATGGCCGATCC	915	0.11947852520409283	No Hit
AGGACTGGGCTCTCAGCTCCTGAAGGCAAATCCCAATTCCAGAGCAAGGA	863	0.11268848879905148	No Hit
AGGACTGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATC	838	0.10942404821970468	No Hit
AGGACTGGGGCTCTCAGCTCCTGAAGGCAAATCCCAATTCCAGAGCAAGG	799	0.10433152091592368	No Hit
AGGACTGGGACCTACTGTATGTAGTTCTGCCAAGATGGTCAAGAACCCCC	769	0.10041419222070753	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACAACTC	20	7.8548334E-4	44.000004	27
TGATACG	20	7.8548334E-4	44.000004	18
GATACGG	20	7.8548334E-4	44.000004	17
ACACGTA	20	7.8548334E-4	44.000004	39
ATAATCG	40	8.305506E-9	44.000004	11
CACATCG	20	7.8548334E-4	44.000004	32
AACTTAC	40	8.305506E-9	44.000004	16
GTCTACC	20	7.8548334E-4	44.000004	11
TACGGGG	20	7.8548334E-4	44.000004	19
TTATCGA	40	8.305506E-9	44.000004	34
GTAATCT	20	7.8548334E-4	44.000004	18
TAGTTAG	20	7.8548334E-4	44.000004	26
AACGGGC	40	8.305506E-9	44.000004	25
CCCAATG	20	7.8548334E-4	44.000004	38
ACTAGGC	20	7.8548334E-4	44.000004	4
CCCCGTA	40	8.305506E-9	44.000004	27
CTTATTA	20	7.8548334E-4	44.000004	30
TACGACA	40	8.305506E-9	44.000004	39
AATCTAC	20	7.8548334E-4	44.000004	30
AACGATC	20	7.8548334E-4	44.000004	23

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content