FastQCFastQC Report
Fri 17 Jun 2016
SRR1527587_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527587_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences593806
Sequences flagged as poor quality0
Sequence length52
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGACTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA77001.296719804111107No Hit
AGGACTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTAGGACTGGGAAG45300.762875417223807No Hit
AGGACTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG44950.7569812362960293No Hit
AGGACTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC34990.5892496876084109No Hit
AGGACTGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAG33480.563820507034284No Hit
AGGACTGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT20250.34102046796428465No Hit
AGGACTGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT20020.3371471490688878No Hit
AGGACTGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC17950.3022872790103165No Hit
AGGACTGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGG17400.2930249946952372No Hit
AGGACTGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCT16210.2729847795407928No Hit
AGGACTGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG15680.2640593055644436No Hit
AGGACTGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCGA15330.25816512463666585No Hit
AGGACTGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC13650.22987305618333262No Hit
AGGACTGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC12030.20259141874618983No Hit
AGGACTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT11630.19585521197158667No Hit
AGGACTGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG10850.18271960876111054No Hit
AGGACTGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCA10810.18204598808365022No Hit
AGGACTGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTG10520.17716223817206292No Hit
AGGACTGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTAC9300.1566168075095233No Hit
AGGACTGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCAT9180.15459594547714237No Hit
AGGACTGGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCT9170.15442754030777728No Hit
AGGACTGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTC8930.1503858162430154No Hit
AGGACTGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC8610.14499685082333288No Hit
AGGACTGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCG8600.1448284456539678No Hit
AGGACTGGGCTCTTTCTTGACTCCATCTTCGCGGTAGCAGCAGCGGCGTCCG8470.14263917845222177No Hit
AGGACTGGGCTCTTTTTCGTGACGCCTCCCAGGCGCTCGGCTGTGTCAAGAT7950.13388210964523767No Hit
AGGACTGGGGCCTCTCCTTCCTGCGGCGCCTTAGGGACCATGGCCGATCCTC7290.12276736846714247No Hit
AGGACTGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCT7260.12226215295904723No Hit
AGGACTGGGCTCTCAGCTCCTGAAGGCAAATCCCAATTCCAGAGCAAGGAAA7190.12108331677349168No Hit
AGGACTGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCGT7090.11939926507984089No Hit
AGGACTGGGAGTCCGGAGTAGGCGAGCGAGCGAGGCAGAGGTTTTTGGTCGG6860.11552594618444408No Hit
AGGACTGGGGCTCTCAGCTCCTGAAGGCAAATCCCAATTCCAGAGCAAGGAA6710.11299986864396788No Hit
AGGACTGGGAGCCGGGCGTGGTGGCGCACGCCTTTGATCCCAGCACTCGGGA6600.11114741178095203No Hit
AGGACTGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCAT6540.11013698076476156No Hit
AGGACTGGGGCTCTATCTGCGGCGTGTGGCGGCGAGATGTCTCACAGGAAAT6380.10744249805492029No Hit
AGGACTGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTGC6180.10407439466761871No Hit
AGGACTGGGACCTACTGTATGTAGTTCTGCCAAGATGGTCAAGAACCCCCCA6080.10239034297396793No Hit
AGGACTGGGGCTCTTTTTCGTGACGCCTCCCAGGCGCTCGGCTGTGTCAAGA6060.10205353263523778No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATAAC351.0184522E-746.00000411
CGTTGAT850.046.00000415
TTCGTGC551.8189894E-1246.00000411
AATTCGG351.0184522E-746.00000445
GGCATAT351.0184522E-746.0000048
CGACTCA351.0184522E-746.00000412
GATTCGT551.8189894E-1246.0000049
CATTTCG351.0184522E-746.00000420
AACGTGT206.308979E-446.013
TAATACC501.6370905E-1146.032
TCGCATA501.6370905E-1146.015
ACACGTA206.308979E-446.039
GATCGTT206.308979E-446.09
TACCACG253.4151464E-546.035
ACGTTGC301.859873E-646.017
GCGAAAC206.308979E-446.015
TTAGATC206.308979E-446.032
CTATAGC206.308979E-446.038
GAATCGT206.308979E-446.045
GTCTAGA253.4151464E-546.030
Produced by FastQC (version 0.11.5)