##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527587_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	593806
Sequences flagged as poor quality	0
Sequence length	52
%GC	57
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.726060363148907	31.0	31.0	34.0	30.0	34.0
2	32.25042859115604	33.0	31.0	34.0	31.0	34.0
3	32.73997736634524	34.0	31.0	34.0	31.0	34.0
4	35.235858175902564	35.0	35.0	37.0	33.0	37.0
5	36.095049561641346	37.0	35.0	37.0	35.0	37.0
6	36.28281122117325	37.0	37.0	37.0	35.0	37.0
7	36.5370541894154	37.0	37.0	37.0	35.0	37.0
8	36.66030488071862	37.0	37.0	37.0	35.0	37.0
9	38.66604749699397	39.0	39.0	39.0	39.0	39.0
10	37.62867333775677	39.0	37.0	39.0	35.0	39.0
11	37.76968572227293	39.0	37.0	39.0	35.0	39.0
12	37.6238670542231	39.0	37.0	39.0	35.0	39.0
13	37.48289003479251	39.0	37.0	39.0	35.0	39.0
14	38.212234298744036	40.0	38.0	40.0	35.0	40.0
15	38.38578593008491	40.0	38.0	40.0	35.0	40.0
16	38.354467620738085	40.0	38.0	40.0	35.0	40.0
17	38.43756883561298	40.0	38.0	40.0	35.0	40.0
18	38.32986362549385	40.0	38.0	40.0	35.0	40.0
19	38.08803548633729	40.0	38.0	40.0	34.0	40.0
20	37.9894426799325	40.0	38.0	40.0	33.0	40.0
21	38.111090154023366	40.0	38.0	40.0	34.0	40.0
22	38.1041585972523	40.0	38.0	40.0	34.0	40.0
23	37.93548061151285	40.0	37.0	40.0	34.0	40.0
24	37.87920634011782	40.0	37.0	40.0	34.0	40.0
25	37.907656709430356	40.0	37.0	40.0	34.0	40.0
26	37.80494976473798	40.0	37.0	40.0	34.0	40.0
27	37.7765364445627	40.0	37.0	40.0	34.0	40.0
28	37.591656197478635	40.0	37.0	40.0	34.0	40.0
29	37.54691262803003	40.0	37.0	40.0	33.0	40.0
30	37.22698659158041	39.0	36.0	40.0	33.0	40.0
31	37.253943207040685	39.0	36.0	40.0	33.0	40.0
32	36.92720686554195	39.0	35.0	40.0	32.0	40.0
33	36.810374095243226	39.0	35.0	40.0	31.0	40.0
34	36.43142036287946	39.0	35.0	40.0	31.0	40.0
35	36.37018655924662	39.0	35.0	40.0	30.0	40.0
36	36.26189193103472	38.0	35.0	40.0	30.0	40.0
37	35.84499988211638	38.0	35.0	40.0	29.0	40.0
38	36.062616410073325	39.0	35.0	40.0	30.0	40.0
39	35.96141837569846	38.0	35.0	40.0	30.0	40.0
40	35.63037759807075	38.0	35.0	40.0	28.0	40.0
41	35.47850476418224	38.0	35.0	40.0	28.0	40.0
42	35.41210597400498	38.0	34.0	40.0	28.0	40.0
43	35.422218704425354	38.0	34.0	40.0	28.0	40.0
44	35.1816081346433	38.0	34.0	40.0	27.0	40.0
45	35.106585315742855	37.0	34.0	40.0	27.0	40.0
46	35.00698544642526	37.0	34.0	40.0	28.0	40.0
47	34.77980518890008	37.0	34.0	40.0	26.0	40.0
48	34.55371451282068	36.0	33.0	40.0	26.0	40.0
49	34.38767712013688	36.0	33.0	40.0	26.0	40.0
50	34.66340521988663	36.0	34.0	40.0	27.0	40.0
51	34.75271048120093	36.0	34.0	40.0	28.0	40.0
52	34.40207913022098	35.0	34.0	39.0	27.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	4.0
12	1.0
13	2.0
14	9.0
15	12.0
16	28.0
17	65.0
18	113.0
19	167.0
20	317.0
21	578.0
22	1034.0
23	1526.0
24	2241.0
25	3091.0
26	4274.0
27	5638.0
28	6906.0
29	8104.0
30	9348.0
31	12585.0
32	17116.0
33	20670.0
34	37073.0
35	40045.0
36	65221.0
37	107952.0
38	203092.0
39	46594.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	100.0	0.0	0.0
2	100.0	0.0	0.0	0.0
3	100.0	0.0	0.0	0.0
4	0.0	100.0	0.0	0.0
5	0.0	0.0	0.0	100.0
6	0.0	0.0	100.0	0.0
7	99.39340457994699	0.4738921465933318	0.11114741178095203	0.02155586167873009
8	99.27653139240762	0.5289606369757126	0.11552594618444408	0.07898202443222196
9	97.75920081642826	1.4391905773939637	0.43482214730063357	0.3667864588771417
10	52.93614412788015	27.747951351114676	4.473851729352684	14.842052791652494
11	30.766614011983712	26.81161860944484	18.783407375472798	23.638360003098654
12	28.751477755361176	17.58570981094836	28.419551166542607	25.243261267147854
13	22.831025621162468	18.92251004536835	29.346453218728	28.900011114741176
14	26.47817637410198	18.973368406516606	30.13846273025197	24.409992489129444
15	29.95052256124054	16.454363883153757	27.704166007079756	25.89094754852595
16	29.815798425748476	20.057055671380887	26.0445330629869	24.082612839883733
17	34.89927686820275	17.765229721491533	19.95230765603581	27.383185754269913
18	32.790170527074494	19.614823696628193	23.001114842221195	24.593890934076114
19	34.06247158162767	19.284917969842002	22.62809739207755	24.024513056452783
20	30.826061036769588	24.91082946282119	21.316221122723583	22.946888377685642
21	33.38144107671529	18.493582078995495	26.221695301158967	21.903281543130248
22	31.551550506394342	18.212345446155815	23.0145872557704	27.22151679167944
23	29.58272567134721	24.491163780763415	21.081295911459296	24.84481463643008
24	29.578683947282446	23.88625241240405	21.051319791312313	25.48374384900119
25	28.60597568902975	19.949781578495333	21.37718379403374	30.06705893844118
26	29.31816115027467	20.659272557030413	20.928384017675807	29.094182275019115
27	27.097233776688007	18.071558724566607	22.604857478705167	32.226350020040215
28	28.117095482362924	23.880863446984367	20.59123686860692	27.410804202045785
29	32.807179449180374	21.809985079301995	20.44270350922692	24.940131962290714
30	29.703303772612603	23.542200651391195	21.151184056745805	25.603311519250394
31	34.36627450716227	20.44640842295295	19.918289811824064	25.26902725806071
32	34.38210459308259	20.1409214457247	22.567639936275484	22.90933402491723
33	36.37315891048591	19.178822713142	21.1729083235939	23.27511005277818
34	29.428634941378164	22.57909148779231	23.450251428917863	24.54202214191167
35	30.816798752454506	26.32930620438325	23.080096866653417	19.773798176508826
36	33.46328598902672	23.094242900880086	19.42452585524565	24.017945254847543
37	30.73815353836101	26.255881550540078	20.670050487869776	22.335914423229138
38	34.28577683620577	22.063434859196438	19.956686190439303	23.694102114158497
39	30.45422242281149	22.477711575834533	19.004523362849145	28.063542638504828
40	29.6792218333934	21.414401336463424	22.289771406823103	26.616605423320074
41	26.893295116586895	22.455987308986437	22.83978268996945	27.81093488445721
42	27.918377382512134	20.688575056499932	24.01845047035564	27.374597090632296
43	29.902021872463397	19.045614224174226	24.01946090137183	27.032903001990547
44	28.438580950680866	20.55839786058073	23.385583843881673	27.61743734485674
45	27.24576713606801	19.783565676332003	22.245986062788184	30.72468112481181
46	26.87780184100531	20.795007123538664	25.557505313183093	26.76968572227293
47	29.403205760804035	20.77833501177152	23.48208000592786	26.336379221496582
48	31.45589637019498	21.170719056392155	22.938973334725482	24.434411238687385
49	31.366978440770215	20.974190223743108	22.692933382283105	24.965897953203573
50	27.156175585965787	22.239418261182944	22.34955524194771	28.254850910903563
51	25.3626605322277	25.34750406698484	22.13382821999104	27.156007180796422
52	29.43318188095102	22.019144299653423	22.760632260367863	25.787041559027696
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	11.5
6	23.0
7	12.5
8	2.0
9	5.0
10	8.0
11	87.0
12	166.0
13	280.5
14	529.0
15	663.0
16	596.5
17	530.0
18	624.5
19	719.0
20	688.0
21	657.0
22	801.0
23	945.0
24	1086.0
25	1227.0
26	1490.5
27	1754.0
28	2289.0
29	2824.0
30	2851.5
31	2879.0
32	3605.5
33	4332.0
34	4582.0
35	4832.0
36	5730.0
37	6628.0
38	7240.5
39	8589.5
40	9326.0
41	10292.5
42	11259.0
43	12301.5
44	13344.0
45	13674.5
46	14005.0
47	14735.5
48	15466.0
49	16807.5
50	18149.0
51	20984.0
52	23819.0
53	27109.0
54	30399.0
55	34809.0
56	39219.0
57	46318.0
58	53417.0
59	59453.0
60	65489.0
61	60897.0
62	56305.0
63	56183.0
64	49974.5
65	43888.0
66	39339.5
67	34791.0
68	28382.5
69	21974.0
70	20059.5
71	18145.0
72	15533.5
73	12922.0
74	11671.5
75	10421.0
76	7977.0
77	5533.0
78	3823.0
79	2113.0
80	1496.0
81	879.0
82	586.0
83	293.0
84	199.0
85	105.0
86	64.0
87	23.0
88	23.0
89	12.0
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	593806.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	11.855555518132183
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.5933464963991	8.487788941169338
2	9.180528132501882	2.176805219213009
3	3.139249137061606	1.1165262728904728
4	1.4815551357263597	0.7025863665911088
5	0.8380800863648631	0.49679524962698257
6	0.5667694143382719	0.40316197545999866
7	0.43466526513160697	0.36072387277999884
8	0.3252887114873791	0.30851827027682444
9	0.29829969175698523	0.318285770099999
>10	8.332504723078452	27.315318471015786
>50	2.5156607338172416	20.4480924746466
>100	1.2244492109262917	27.138493043182454
>500	0.044034716402221626	3.518320798375227
>1k	0.024148070285089276	5.911863470561092
>5k	0.0014204747226523103	1.296719804111107
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AGGACTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA	7700	1.296719804111107	No Hit
AGGACTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTAGGACTGGGAAG	4530	0.762875417223807	No Hit
AGGACTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG	4495	0.7569812362960293	No Hit
AGGACTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	3499	0.5892496876084109	No Hit
AGGACTGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAG	3348	0.563820507034284	No Hit
AGGACTGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT	2025	0.34102046796428465	No Hit
AGGACTGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT	2002	0.3371471490688878	No Hit
AGGACTGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC	1795	0.3022872790103165	No Hit
AGGACTGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGG	1740	0.2930249946952372	No Hit
AGGACTGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCT	1621	0.2729847795407928	No Hit
AGGACTGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG	1568	0.2640593055644436	No Hit
AGGACTGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCGA	1533	0.25816512463666585	No Hit
AGGACTGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC	1365	0.22987305618333262	No Hit
AGGACTGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC	1203	0.20259141874618983	No Hit
AGGACTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	1163	0.19585521197158667	No Hit
AGGACTGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG	1085	0.18271960876111054	No Hit
AGGACTGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCA	1081	0.18204598808365022	No Hit
AGGACTGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTG	1052	0.17716223817206292	No Hit
AGGACTGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTAC	930	0.1566168075095233	No Hit
AGGACTGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCAT	918	0.15459594547714237	No Hit
AGGACTGGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCT	917	0.15442754030777728	No Hit
AGGACTGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTC	893	0.1503858162430154	No Hit
AGGACTGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC	861	0.14499685082333288	No Hit
AGGACTGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCG	860	0.1448284456539678	No Hit
AGGACTGGGCTCTTTCTTGACTCCATCTTCGCGGTAGCAGCAGCGGCGTCCG	847	0.14263917845222177	No Hit
AGGACTGGGCTCTTTTTCGTGACGCCTCCCAGGCGCTCGGCTGTGTCAAGAT	795	0.13388210964523767	No Hit
AGGACTGGGGCCTCTCCTTCCTGCGGCGCCTTAGGGACCATGGCCGATCCTC	729	0.12276736846714247	No Hit
AGGACTGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCT	726	0.12226215295904723	No Hit
AGGACTGGGCTCTCAGCTCCTGAAGGCAAATCCCAATTCCAGAGCAAGGAAA	719	0.12108331677349168	No Hit
AGGACTGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCGT	709	0.11939926507984089	No Hit
AGGACTGGGAGTCCGGAGTAGGCGAGCGAGCGAGGCAGAGGTTTTTGGTCGG	686	0.11552594618444408	No Hit
AGGACTGGGGCTCTCAGCTCCTGAAGGCAAATCCCAATTCCAGAGCAAGGAA	671	0.11299986864396788	No Hit
AGGACTGGGAGCCGGGCGTGGTGGCGCACGCCTTTGATCCCAGCACTCGGGA	660	0.11114741178095203	No Hit
AGGACTGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCAT	654	0.11013698076476156	No Hit
AGGACTGGGGCTCTATCTGCGGCGTGTGGCGGCGAGATGTCTCACAGGAAAT	638	0.10744249805492029	No Hit
AGGACTGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTGC	618	0.10407439466761871	No Hit
AGGACTGGGACCTACTGTATGTAGTTCTGCCAAGATGGTCAAGAACCCCCCA	608	0.10239034297396793	No Hit
AGGACTGGGGCTCTTTTTCGTGACGCCTCCCAGGCGCTCGGCTGTGTCAAGA	606	0.10205353263523778	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0011788361855555518	0.0	0.0	0.0	0.0
8	0.001852456863015867	0.0	0.0	0.0	0.0
9	0.002694482709841261	0.0	0.0	0.0	0.0
10	0.018356163460793593	0.0	0.0	0.0	0.0
11	0.04715344742222207	0.0	0.0	0.0	0.0
12	0.0739298693512696	0.0	0.0	0.0	0.0
13	0.0922860328120632	0.0	0.0	0.0	0.0
14	0.11434710999888853	0.0	0.0	0.0	0.0
15	0.12529344600761866	0.0	0.0	0.0	0.0
16	0.1352293510001583	0.0	0.0	0.0	0.0
17	0.14499685082333288	0.0	0.0	0.0	0.0
18	0.1534171092915868	0.0	0.0	0.0	0.0
19	0.16284779877603123	0.0	0.0	0.0	0.0
20	0.17850947952698357	0.0	0.0	0.0	0.0
21	0.19029784138253908	0.0	0.0	0.0	0.0
22	0.20292822908492	0.0	0.0	0.0	0.0
23	0.22178960805380882	0.0	0.0	0.0	0.0
24	0.24536633176491984	0.0	0.0	0.0	0.0
25	0.2610280125158722	0.0	0.0	0.0	0.0
26	0.27820533979111023	0.0	0.0	0.0	0.0
27	0.2992559859617451	0.0	0.0	0.0	0.0
28	0.4095613718958717	0.0	0.0	0.0	0.0
29	0.4407163282284113	0.0	0.0	0.0	0.0
30	0.4841648619246016	0.0	0.0	0.0	0.0
31	0.5030262408934905	0.0	0.0	0.0	0.0
32	0.5166670596120618	0.0	0.0	0.0	0.0
33	0.5274449904514269	0.0	0.0	0.0	0.0
34	0.549169257299522	0.0	0.0	0.0	0.0
35	0.6352242988450774	0.0	0.0	0.0	0.0
36	0.6657056345001566	0.0	0.0	0.0	0.0
37	0.6817041255898391	0.0	0.0	0.0	0.0
38	0.6894507633806327	0.0	0.0	0.0	0.0
39	0.7037652027766644	0.0	0.0	0.0	0.0
40	0.719763693866347	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATAAC	35	1.0184522E-7	46.000004	11
CGTTGAT	85	0.0	46.000004	15
TTCGTGC	55	1.8189894E-12	46.000004	11
AATTCGG	35	1.0184522E-7	46.000004	45
GGCATAT	35	1.0184522E-7	46.000004	8
CGACTCA	35	1.0184522E-7	46.000004	12
GATTCGT	55	1.8189894E-12	46.000004	9
CATTTCG	35	1.0184522E-7	46.000004	20
AACGTGT	20	6.308979E-4	46.0	13
TAATACC	50	1.6370905E-11	46.0	32
TCGCATA	50	1.6370905E-11	46.0	15
ACACGTA	20	6.308979E-4	46.0	39
GATCGTT	20	6.308979E-4	46.0	9
TACCACG	25	3.4151464E-5	46.0	35
ACGTTGC	30	1.859873E-6	46.0	17
GCGAAAC	20	6.308979E-4	46.0	15
TTAGATC	20	6.308979E-4	46.0	32
CTATAGC	20	6.308979E-4	46.0	38
GAATCGT	20	6.308979E-4	46.0	45
GTCTAGA	25	3.4151464E-5	46.0	30
>>END_MODULE