FastQCFastQC Report
Fri 17 Jun 2016
SRR1527586_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527586_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences786676
Sequences flagged as poor quality0
Sequence length52
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGACTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA91451.162486207790755No Hit
AGGACTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTAGGACTGGGAAG72720.924395812253075No Hit
AGGACTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG61870.7864737198033245No Hit
AGGACTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC47410.6026623412942559No Hit
AGGACTGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAG42430.5393580076168588No Hit
AGGACTGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT26810.3408010413435773No Hit
AGGACTGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT26500.33686041013072726No Hit
AGGACTGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC25390.32275040804600624No Hit
AGGACTGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCT23040.2928778811098851No Hit
AGGACTGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGG20890.2655476968917318No Hit
AGGACTGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC20370.2589376058250156No Hit
AGGACTGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCGA19940.25347156898138495No Hit
AGGACTGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG19410.24673436077877042No Hit
AGGACTGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC17370.22080246505549933No Hit
AGGACTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT15860.2016077775348428No Hit
AGGACTGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG14410.1831757928295766No Hit
AGGACTGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCA14180.18025209870391368No Hit
AGGACTGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC12910.16410822244481846No Hit
AGGACTGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTG12790.1625828168140378No Hit
AGGACTGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCAT12710.1615658797268507No Hit
AGGACTGGGCTCTTTCTTGACTCCATCTTCGCGGTAGCAGCAGCGGCGTCCG11560.1469474090985361No Hit
AGGACTGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTAC11400.14491353492416192No Hit
AGGACTGGGCTCTTTTTCGTGACGCCTCCCAGGCGCTCGGCTGTGTCAAGAT11000.1398288494882264No Hit
AGGACTGGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCT10740.13652380395486832No Hit
AGGACTGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCG10450.1328374070138151No Hit
AGGACTGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTC10230.13004083002405056No Hit
AGGACTGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCGT9920.12610019881120055No Hit
AGGACTGGGGCCTCTCCTTCCTGCGGCGCCTTAGGGACCATGGCCGATCCTC9850.12521037885991185No Hit
AGGACTGGGCTCTCAGCTCCTGAAGGCAAATCCCAATTCCAGAGCAAGGAAA9770.12419344177272472No Hit
AGGACTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTAGGACTGGGGAG9640.12254091900604569No Hit
AGGACTGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCT9580.12177821619065536No Hit
AGGACTGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCAT8570.1089393854649182No Hit
AGGACTGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTC8410.10690551129054401No Hit
AGGACTGGGGCTCTCAGCTCCTGAAGGCAAATCCCAATTCCAGAGCAAGGAA8390.10665127701874723No Hit
AGGACTGGGAGCCGGGCGTGGTGGCGCACGCCTTTGATCCCAGCACTCGGGA8350.10614280847515369No Hit
AGGACTGGGACCTACTGTATGTAGTTCTGCCAAGATGGTCAAGAACCCCCCA8250.1048716371161698No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTTAG551.8189894E-1246.00000426
GCGTACA551.8189894E-1246.00000442
TCACGCC301.8606479E-646.031
ACACGTA301.8606479E-646.039
GATCGTT253.4161654E-546.09
ATCTAAG301.8606479E-646.038
CCGATTC253.4161654E-546.029
ACGCATT253.4161654E-546.035
ACGTTAA453.092282E-1046.015
GTTTACG206.3102343E-446.046
GGCAATT253.4161654E-546.08
CTAAGTC206.3102343E-446.028
ATAGACG301.8606479E-646.038
TGACGTT206.3102343E-446.017
CGACAAA206.3102343E-446.011
ACTTAGC206.3102343E-446.041
ACTTACG253.4161654E-546.046
TTATCGA301.8606479E-646.034
GTTACTA206.3102343E-446.019
GCATTAC301.8606479E-646.019
Produced by FastQC (version 0.11.5)