##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527586_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	786676
Sequences flagged as poor quality	0
Sequence length	52
%GC	57
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.91090487062018	31.0	30.0	33.0	26.0	33.0
2	31.06046072334735	31.0	31.0	33.0	28.0	34.0
3	31.73825691898571	31.0	31.0	34.0	30.0	34.0
4	34.16880520061626	35.0	35.0	37.0	30.0	37.0
5	35.452580223624466	35.0	35.0	37.0	35.0	37.0
6	35.83464602962338	37.0	35.0	37.0	35.0	37.0
7	36.27814754740198	37.0	35.0	37.0	35.0	37.0
8	36.47859220314335	37.0	37.0	37.0	35.0	37.0
9	38.54753418179784	39.0	39.0	39.0	37.0	39.0
10	37.013980342606104	39.0	37.0	39.0	33.0	39.0
11	37.1053356146622	39.0	37.0	39.0	34.0	39.0
12	36.984433235537885	39.0	37.0	39.0	33.0	39.0
13	36.83785192379074	39.0	37.0	39.0	33.0	39.0
14	37.51624938348189	40.0	37.0	40.0	33.0	40.0
15	37.70337851923791	40.0	37.0	40.0	33.0	40.0
16	37.584752808017534	40.0	37.0	40.0	33.0	40.0
17	37.748464424998346	40.0	37.0	40.0	33.0	40.0
18	37.673906411279866	40.0	37.0	40.0	33.0	40.0
19	37.35062338243445	39.0	36.0	40.0	32.0	40.0
20	37.21246739445464	39.0	36.0	40.0	32.0	40.0
21	37.41599845425563	39.0	37.0	40.0	32.0	40.0
22	37.428084751536844	39.0	36.0	40.0	33.0	40.0
23	37.283822056348484	39.0	36.0	40.0	32.0	40.0
24	37.296289705037395	39.0	36.0	40.0	32.0	40.0
25	37.30447986210333	39.0	36.0	40.0	33.0	40.0
26	37.18137072949982	39.0	36.0	40.0	32.0	40.0
27	37.11913417976397	39.0	36.0	40.0	32.0	40.0
28	36.825343597618335	39.0	35.0	40.0	31.0	40.0
29	36.86015589645547	39.0	35.0	40.0	31.0	40.0
30	36.60042380853108	39.0	35.0	40.0	31.0	40.0
31	36.535835591781115	38.0	35.0	40.0	31.0	40.0
32	36.22263676532651	38.0	35.0	40.0	30.0	40.0
33	36.12119983322232	38.0	35.0	40.0	30.0	40.0
34	35.78207038221581	38.0	35.0	40.0	29.0	40.0
35	35.73344807773467	38.0	34.0	40.0	29.0	40.0
36	35.656868901555406	38.0	34.0	40.0	29.0	40.0
37	35.33714515251514	38.0	34.0	40.0	28.0	40.0
38	35.28401654556641	38.0	34.0	40.0	28.0	40.0
39	35.10472545240989	38.0	34.0	40.0	27.0	40.0
40	35.237009899882544	38.0	34.0	40.0	27.0	40.0
41	35.261455796287166	38.0	34.0	40.0	27.0	40.0
42	35.341767131576404	38.0	34.0	40.0	28.0	40.0
43	35.34444040494435	38.0	34.0	40.0	28.0	40.0
44	35.15308843793379	37.0	34.0	40.0	28.0	40.0
45	35.108151259222346	37.0	34.0	40.0	28.0	40.0
46	34.910308691252816	37.0	34.0	40.0	27.0	40.0
47	34.7923567008527	37.0	34.0	40.0	27.0	40.0
48	34.61133427230524	36.0	34.0	40.0	26.0	40.0
49	34.4381460728432	36.0	33.0	40.0	27.0	40.0
50	34.16832469784257	35.0	33.0	39.0	26.0	40.0
51	34.010244369982054	35.0	33.0	39.0	26.0	40.0
52	33.58027447131983	35.0	33.0	39.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	0.0
13	5.0
14	8.0
15	24.0
16	44.0
17	73.0
18	175.0
19	313.0
20	556.0
21	967.0
22	1640.0
23	2524.0
24	3753.0
25	5226.0
26	7302.0
27	10191.0
28	12280.0
29	14297.0
30	17064.0
31	21416.0
32	28835.0
33	38414.0
34	57553.0
35	61288.0
36	99718.0
37	153846.0
38	224118.0
39	25045.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	100.0	0.0	0.0
2	100.0	0.0	0.0	0.0
3	100.0	0.0	0.0	0.0
4	0.0	100.0	0.0	0.0
5	0.0	0.0	0.0	100.0
6	0.0	0.0	100.0	0.0
7	99.41805775185718	0.4681724115137617	0.09190568925453427	0.021864147374522674
8	99.32221143138979	0.5163498060192506	0.10245641153410046	0.05898235105685187
9	97.77354336473975	1.4337541757979142	0.4085544747774179	0.3841479846849275
10	52.582130381503944	28.21288560983175	4.384651368543085	14.82033264012122
11	30.215107617367252	27.224676995357683	18.82363260096914	23.736582786305927
12	28.909360397419015	17.484707808551427	28.44906924833095	25.156862545698612
13	22.589350634822978	18.784226288840642	29.140077999074588	29.486345077261795
14	26.20773990817058	19.324855467816484	29.899984237475145	24.567420386537787
15	30.198455272564562	16.344721333814682	27.40696296823597	26.049860425384786
16	29.624012935439747	19.890908073972003	26.295832083348163	24.189246907240083
17	35.19339601055581	17.669154772739983	19.71243561517067	27.425013601533543
18	32.9148213495772	19.658792183821546	22.921634828061364	24.504751638539883
19	33.678159750647026	19.150704991635692	22.9818883504772	24.189246907240083
20	30.600781007682958	25.088854877992972	21.32389954695453	22.986464567369538
21	33.229436260925716	18.362832983337483	26.567608519898915	21.84012223583788
22	31.39704274695046	18.066014471014753	22.883626804427745	27.653315977607047
23	29.38109208873793	24.98588999791528	20.883311553930717	24.749706359416074
24	29.438676151299898	24.133696718852487	20.988183191046886	25.439443938800725
25	28.194834976534178	19.805866710055984	21.292628731523525	30.706669581886313
26	29.45685390173337	20.65373800649823	20.933268588338784	28.95613950342962
27	26.96243434399931	18.012879508209227	22.457021696352754	32.56766445143871
28	27.975685034245355	24.370515943031183	20.52433276215367	27.12946626056979
29	33.14592030264048	21.637243286944052	20.335309581072767	24.881526829342704
30	29.53579364312627	23.89789443176098	20.973946071826266	25.592365853286488
31	34.658868454103086	20.35247039441905	19.78285850845837	25.20580264301949
32	33.977901957095426	20.094803959953015	22.9976508753286	22.92964320762296
33	36.59206585684577	19.27222897355455	21.135003483009523	23.00070168659016
34	29.41579506683819	22.46858935571951	23.220232980286674	24.895382597155628
35	30.58552695137515	26.634472133381472	22.950236183638502	19.82976473160488
36	33.36290925361903	23.153877835347718	19.404303677752978	24.07890923328028
37	30.392308904809607	26.164901433372822	21.022886169147146	22.419903492670425
38	34.520183658837944	22.042492716188114	19.86306942121026	23.574254203763683
39	30.223497348336547	22.55350360249963	18.907784144933874	28.31521490422995
40	29.40473587601503	21.480507858381344	22.669053079031265	26.445703186572363
41	26.639048350273807	22.88438950724313	22.724476150282964	27.752085992200094
42	27.79644987262863	20.698101886926764	23.9750545332513	27.53039370719331
43	30.022270922209398	19.062485699322213	23.733531975044365	27.181711403424025
44	28.14030172523377	20.970768143428806	23.351163630262015	27.53776650107541
45	27.17192338395985	19.770909497683924	22.071475423172945	30.985691695183277
46	26.727140525451393	20.635687373200657	26.013250690246046	26.623921411101904
47	29.441981196833254	20.665941251544474	23.52328023226843	26.36879731935384
48	31.556066283959343	20.98144598284427	22.998922046687582	24.463565686508804
49	31.262171465762272	21.033436891426714	22.681637675485206	25.022753967325812
50	27.260778261952822	22.078721099919154	22.439479531598778	28.221021106529243
51	25.25575967742756	25.250547874855723	22.167830212183922	27.3258622355328
52	29.50795499036452	21.936604141984756	22.594181085987117	25.96125978166361
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	12.5
6	25.0
7	20.5
8	16.0
9	15.5
10	15.0
11	114.0
12	213.0
13	377.0
14	684.5
15	828.0
16	769.0
17	710.0
18	816.5
19	923.0
20	873.5
21	824.0
22	1140.5
23	1457.0
24	1554.5
25	1652.0
26	1995.0
27	2338.0
28	3055.5
29	3773.0
30	3817.5
31	3862.0
32	4793.0
33	5724.0
34	5918.0
35	6112.0
36	7370.5
37	8629.0
38	9517.0
39	11189.5
40	11974.0
41	13501.0
42	15028.0
43	16223.0
44	17418.0
45	17977.0
46	18536.0
47	19616.5
48	20697.0
49	22242.5
50	23788.0
51	27680.0
52	31572.0
53	36211.0
54	40850.0
55	47778.0
56	54706.0
57	63219.5
58	71733.0
59	79160.5
60	86588.0
61	80163.0
62	73738.0
63	73434.5
64	65232.5
65	57334.0
66	51261.0
67	45188.0
68	37210.0
69	29232.0
70	26644.0
71	24056.0
72	20581.0
73	17106.0
74	15676.5
75	14247.0
76	10805.5
77	7364.0
78	5067.0
79	2770.0
80	1936.0
81	1102.0
82	695.5
83	289.0
84	208.5
85	128.0
86	80.5
87	33.0
88	25.5
89	10.5
90	3.0
91	1.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	786676.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	11.491134850942448
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.92749839598221	8.380197184101206
2	9.653974645456758	2.218702489970458
3	3.589681187636894	1.2374853179708036
4	1.5542379256178234	0.7143983037489385
5	0.8650633863581053	0.4970280013626957
6	0.5199230071461758	0.3584703232334532
7	0.40819487156795503	0.32834356202553533
8	0.27655479103519987	0.25423427179677527
9	0.2345184627978495	0.24253949529412364
>10	5.614062258014558	18.476221468558848
>50	2.810902896081772	22.505199090858245
>100	1.4469346666961658	31.152215143210167
>500	0.06969180734087037	5.196421398390188
>1k	0.025443040775238394	5.565188209631411
>5k	0.003318657492422399	2.8733557398471543
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AGGACTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA	9145	1.162486207790755	No Hit
AGGACTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTAGGACTGGGAAG	7272	0.924395812253075	No Hit
AGGACTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG	6187	0.7864737198033245	No Hit
AGGACTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	4741	0.6026623412942559	No Hit
AGGACTGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAG	4243	0.5393580076168588	No Hit
AGGACTGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT	2681	0.3408010413435773	No Hit
AGGACTGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT	2650	0.33686041013072726	No Hit
AGGACTGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC	2539	0.32275040804600624	No Hit
AGGACTGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCT	2304	0.2928778811098851	No Hit
AGGACTGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGG	2089	0.2655476968917318	No Hit
AGGACTGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC	2037	0.2589376058250156	No Hit
AGGACTGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCGA	1994	0.25347156898138495	No Hit
AGGACTGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG	1941	0.24673436077877042	No Hit
AGGACTGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC	1737	0.22080246505549933	No Hit
AGGACTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	1586	0.2016077775348428	No Hit
AGGACTGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG	1441	0.1831757928295766	No Hit
AGGACTGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCA	1418	0.18025209870391368	No Hit
AGGACTGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC	1291	0.16410822244481846	No Hit
AGGACTGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTG	1279	0.1625828168140378	No Hit
AGGACTGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCAT	1271	0.1615658797268507	No Hit
AGGACTGGGCTCTTTCTTGACTCCATCTTCGCGGTAGCAGCAGCGGCGTCCG	1156	0.1469474090985361	No Hit
AGGACTGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTAC	1140	0.14491353492416192	No Hit
AGGACTGGGCTCTTTTTCGTGACGCCTCCCAGGCGCTCGGCTGTGTCAAGAT	1100	0.1398288494882264	No Hit
AGGACTGGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCT	1074	0.13652380395486832	No Hit
AGGACTGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCG	1045	0.1328374070138151	No Hit
AGGACTGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTC	1023	0.13004083002405056	No Hit
AGGACTGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCGT	992	0.12610019881120055	No Hit
AGGACTGGGGCCTCTCCTTCCTGCGGCGCCTTAGGGACCATGGCCGATCCTC	985	0.12521037885991185	No Hit
AGGACTGGGCTCTCAGCTCCTGAAGGCAAATCCCAATTCCAGAGCAAGGAAA	977	0.12419344177272472	No Hit
AGGACTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTAGGACTGGGGAG	964	0.12254091900604569	No Hit
AGGACTGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCT	958	0.12177821619065536	No Hit
AGGACTGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCAT	857	0.1089393854649182	No Hit
AGGACTGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTC	841	0.10690551129054401	No Hit
AGGACTGGGGCTCTCAGCTCCTGAAGGCAAATCCCAATTCCAGAGCAAGGAA	839	0.10665127701874723	No Hit
AGGACTGGGAGCCGGGCGTGGTGGCGCACGCCTTTGATCCCAGCACTCGGGA	835	0.10614280847515369	No Hit
AGGACTGGGACCTACTGTATGTAGTTCTGCCAAGATGGTCAAGAACCCCCCA	825	0.1048716371161698	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	3.8135140769516294E-4	0.0	0.0	0.0	0.0
8	6.355856794919382E-4	0.0	0.0	0.0	0.0
9	0.0019067570384758148	0.0	0.0	0.0	0.0
10	0.018177750433469433	0.0	0.0	0.0	0.0
11	0.0422028891182647	0.0	0.0	0.0	0.0
12	0.06292298226970189	0.0	0.0	0.0	0.0
13	0.07728721862621969	0.0	0.0	0.0	0.0
14	0.09965983454433591	0.0	0.0	0.0	0.0
15	0.11071902536749564	0.0	0.0	0.0	0.0
16	0.11961722488038277	0.0	0.0	0.0	0.0
17	0.12889677580096506	0.0	0.0	0.0	0.0
18	0.13804920958564898	0.0	0.0	0.0	0.0
19	0.14859993186521517	0.0	0.0	0.0	0.0
20	0.16182011399864749	0.0	0.0	0.0	0.0
21	0.17211660200641687	0.0	0.0	0.0	0.0
22	0.18457408132445885	0.0	0.0	0.0	0.0
23	0.20008237190406217	0.0	0.0	0.0	0.0
24	0.22003976224010902	0.0	0.0	0.0	0.0
25	0.23643787277100103	0.0	0.0	0.0	0.0
26	0.25359868611728337	0.0	0.0	0.0	0.0
27	0.2736831935892286	0.0	0.0	0.0	0.0
28	0.3821141105105533	0.0	0.0	0.0	0.0
29	0.4076646548261292	0.0	0.0	0.0	0.0
30	0.4506302467597842	0.0	0.0	0.0	0.0
31	0.4660114202034891	0.0	0.0	0.0	0.0
32	0.4802485394241085	0.0	0.0	0.0	0.0
33	0.49016367602418276	0.0	0.0	0.0	0.0
34	0.5092312464089409	0.0	0.0	0.0	0.0
35	0.6005013499839832	0.0	0.0	0.0	0.0
36	0.6251620743482704	0.0	0.0	0.0	0.0
37	0.6359670308996334	0.0	0.0	0.0	0.0
38	0.6453736989561141	0.0	0.0	0.0	0.0
39	0.6574498268664609	0.0	0.0	0.0	0.0
40	0.675500460164032	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGTTAG	55	1.8189894E-12	46.000004	26
GCGTACA	55	1.8189894E-12	46.000004	42
TCACGCC	30	1.8606479E-6	46.0	31
ACACGTA	30	1.8606479E-6	46.0	39
GATCGTT	25	3.4161654E-5	46.0	9
ATCTAAG	30	1.8606479E-6	46.0	38
CCGATTC	25	3.4161654E-5	46.0	29
ACGCATT	25	3.4161654E-5	46.0	35
ACGTTAA	45	3.092282E-10	46.0	15
GTTTACG	20	6.3102343E-4	46.0	46
GGCAATT	25	3.4161654E-5	46.0	8
CTAAGTC	20	6.3102343E-4	46.0	28
ATAGACG	30	1.8606479E-6	46.0	38
TGACGTT	20	6.3102343E-4	46.0	17
CGACAAA	20	6.3102343E-4	46.0	11
ACTTAGC	20	6.3102343E-4	46.0	41
ACTTACG	25	3.4161654E-5	46.0	46
TTATCGA	30	1.8606479E-6	46.0	34
GTTACTA	20	6.3102343E-4	46.0	19
GCATTAC	30	1.8606479E-6	46.0	19
>>END_MODULE