SRR1527585_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR1527585_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2781506
Sequences flagged as poor quality	0
Sequence length	50
%GC	46

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CAAAGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	36749	1.321190750622145	No Hit
CAAAGTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCAAAGTGGGA	26623	0.9571433604673153	No Hit
CAAAGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	14785	0.5315465794429349	No Hit
CAAAGTGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	11263	0.4049245264975161	No Hit
CAAAGTGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC	8924	0.32083339025693275	No Hit
CAAAGTGGGGAGTGCAGTGCTCAAAGTGGGAAAAAAAAAAAAAAAAAAAA	7552	0.27150759336848457	No Hit
CAAAGTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT	6058	0.2177956833456408	No Hit
CAAAGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	5659	0.20345093629134722	No Hit
CAAAGTGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAG	5515	0.19827388472288035	No Hit
CAAAGTGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA	5398	0.194067530323501	Illumina Single End Adapter 2 (97% over 34bp)
CAAAGTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC	5361	0.19273731568438104	No Hit
CAAAGTGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTG	5165	0.1856907732717456	No Hit
CAAAGTGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCC	5149	0.1851155453196937	No Hit
CAAAGTGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAA	5102	0.18342581321054136	No Hit
CAAAGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG	4812	0.17299980657960112	No Hit
CAAAGTGGGGTGCAGTGCTCAAAGTGGGAAAAAAAAAAAAAAAAAAAAAA	4770	0.17148983320546496	No Hit
CAAAGTGGGCAGAGTGCAGTGCTCAAAGTGGGAAAAAAAAAAAAAAAAAA	4437	0.15951790145338532	No Hit
CAAAGTGGGGCAGAGTGCAGTGCTCAAAGTGGGAAAAAAAAAAAAAAAAA	4425	0.15908648048934643	No Hit
CAAAGTGGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAA	4390	0.15782816934423294	Illumina Single End Adapter 2 (97% over 34bp)
CAAAGTGGGAGTGCAGTGCTCAAAGTGGGAAAAAAAAAAAAAAAAAAAAA	4127	0.14837285988238028	No Hit
CAAAGTGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGA	4093	0.14715050048427003	No Hit
CAAAGTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3840	0.13805470849244977	No Hit
CAAAGTGGGGAGTGCAGTGCTCAAAGTGGGGAAAAAAAAAAAAAAAAAAA	3497	0.12572325927033773	No Hit
CAAAGTGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGA	3168	0.11389513450627106	No Hit
CAAAGTGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCA	3060	0.11001234582992091	No Hit
CAAAGTGGGGAGTGCAGTGCTCAAAGTGGGTAAAAAAAAAAAAAAAAAAA	3023	0.10868213119080096	No Hit
CAAAGTGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2991	0.10753167528669721	No Hit
CAAAGTGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCC	2982	0.10720810956366804	No Hit
CAAAGTGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAG	2972	0.10684859209363562	No Hit
CAAAGTGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2927	0.10523076347848971	No Hit
CAAAGTGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATC	2907	0.10451172853842487	No Hit
CAAAGTGGGGGAGTGGCCTTGTTCTCGATACTTCGTTGTGGTTGTGAACT	2832	0.1018153475131817	No Hit
CAAAGTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT	2795	0.10048513287406176	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GATAACG	20	7.858412E-4	44.0	9
TTATCGA	20	7.858412E-4	44.0	28
GTTATCG	20	7.858412E-4	44.0	27
TGGGATT	3360	0.0	41.57738	6
GTGGGAT	11520	0.0	40.77257	5
TGGGGTC	5530	0.0	40.499096	6
TAATTGG	60	3.6379788E-12	40.333332	14
TGGGGCT	9930	0.0	40.300102	6
GTGGGAC	8635	0.0	40.229298	5
TAGTTCG	55	7.8216544E-11	40.0	37
TGGGGTT	4395	0.0	39.895336	6
TGGGGAC	5990	0.0	39.813023	6
TGGGGGC	10155	0.0	39.688824	6
GGAAGCA	16180	0.0	39.64895	8
GCGTAAC	50	1.3496901E-9	39.6	36
TGGGGGT	8880	0.0	39.565315	6
GTGGGGC	27390	0.0	39.421688	5
TAAGGTA	190	0.0	39.368423	33
TGGGACT	2805	0.0	39.294117	6
AGTGGGG	155775	0.0	39.172783	4

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content